Allium virus X
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C0L9E3|C0L9E3_9VIRU Tgb2 OS=Allium virus X OX=317027 PE=4 SV=1
MM1 pKa = 6.83 NTLVDD6 pKa = 3.93 TLVEE10 pKa = 4.03 AGFIRR15 pKa = 11.84 THH17 pKa = 6.4 EE18 pKa = 4.37 PLSNPLVVHH27 pKa = 6.45 AVAGAGKK34 pKa = 10.02 SSLIRR39 pKa = 11.84 RR40 pKa = 11.84 LLEE43 pKa = 3.58 TDD45 pKa = 2.75 ATFRR49 pKa = 11.84 AFTRR53 pKa = 11.84 GPPDD57 pKa = 4.25 PPSLDD62 pKa = 3.08 ATAIQAFQPNPPSHH76 pKa = 7.03 LFNILDD82 pKa = 4.06 EE83 pKa = 4.54 YY84 pKa = 10.53 PAGEE88 pKa = 4.58 VKK90 pKa = 10.82 GPWAALFADD99 pKa = 4.81 PLQHH103 pKa = 6.62 RR104 pKa = 11.84 AHH106 pKa = 7.18 PRR108 pKa = 11.84 RR109 pKa = 11.84 PHH111 pKa = 5.97 FIKK114 pKa = 10.47 RR115 pKa = 11.84 CSHH118 pKa = 6.45 RR119 pKa = 11.84 LSASTAALLTSLGIPITGTGQGNFTSAHH147 pKa = 5.57 GVFEE151 pKa = 4.59 GPLIGKK157 pKa = 9.52 IISLDD162 pKa = 3.43 NHH164 pKa = 6.32 ISLLLSNHH172 pKa = 5.55 SVPYY176 pKa = 8.87 ATPDD180 pKa = 3.36 DD181 pKa = 3.9 VLGQEE186 pKa = 4.51 FPITTVISALPLHH199 pKa = 6.47 AVCDD203 pKa = 4.02 KK204 pKa = 10.6 VGLYY208 pKa = 9.8 IALSRR213 pKa = 11.84 HH214 pKa = 4.35 TSEE217 pKa = 5.54 LHH219 pKa = 5.31 VLCPNPPHH227 pKa = 6.82 PTTT230 pKa = 4.01
Molecular weight: 24.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.42
IPC2_protein 6.224
IPC_protein 6.389
Toseland 6.825
ProMoST 6.766
Dawson 6.751
Bjellqvist 6.693
Wikipedia 6.781
Rodwell 6.751
Grimsley 7.0
Solomon 6.766
Lehninger 6.766
Nozaki 7.015
DTASelect 7.22
Thurlkill 7.249
EMBOSS 7.234
Sillero 7.146
Patrickios 4.342
IPC_peptide 6.781
IPC2_peptide 7.102
IPC2.peptide.svr19 6.938
Protein with the highest isoelectric point:
>tr|C0L9E4|C0L9E4_9VIRU Movement protein TGBp3 OS=Allium virus X OX=317027 PE=3 SV=1
MM1 pKa = 7.42 SSAPIHH7 pKa = 5.85 LTPPPDD13 pKa = 3.29 HH14 pKa = 7.08 SKK16 pKa = 10.46 VFISVVVGVSIALCVFLLNKK36 pKa = 10.32 NYY38 pKa = 10.27 LPHH41 pKa = 8.18 VGDD44 pKa = 4.23 NLHH47 pKa = 6.43 SLPHH51 pKa = 6.52 GGTYY55 pKa = 10.35 CDD57 pKa = 3.47 GTKK60 pKa = 10.3 SINYY64 pKa = 8.48 RR65 pKa = 11.84 GPSHH69 pKa = 6.34 HH70 pKa = 7.04 TSSTTPLWAVIATLTLPLAIFLLNARR96 pKa = 11.84 RR97 pKa = 11.84 TSHH100 pKa = 6.98 HH101 pKa = 5.23 STLRR105 pKa = 11.84 CGHH108 pKa = 7.07 AACASGLDD116 pKa = 3.76 STNPP120 pKa = 3.38
Molecular weight: 12.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.09
IPC2_protein 7.863
IPC_protein 7.907
Toseland 7.614
ProMoST 8.404
Dawson 8.434
Bjellqvist 8.843
Wikipedia 8.361
Rodwell 8.434
Grimsley 7.6
Solomon 8.653
Lehninger 8.668
Nozaki 9.107
DTASelect 8.551
Thurlkill 8.595
EMBOSS 8.668
Sillero 8.931
Patrickios 3.605
IPC_peptide 8.653
IPC2_peptide 8.317
IPC2.peptide.svr19 8.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2359
83
1701
471.8
52.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.351 ± 0.688
1.78 ± 0.454
4.621 ± 0.6
5.087 ± 1.187
4.833 ± 0.681
4.833 ± 0.522
4.451 ± 0.927
4.663 ± 0.435
5.256 ± 1.068
10.725 ± 0.641
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.865 ± 0.423
4.154 ± 0.293
7.164 ± 1.107
4.621 ± 0.907
4.409 ± 0.53
6.825 ± 0.694
7.885 ± 1.028
4.663 ± 0.717
1.145 ± 0.179
2.671 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here