Hubei toti-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFE7|A0A1L3KFE7_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 21 OX=1923310 PE=4 SV=1
MM1 pKa = 7.4IKK3 pKa = 10.25KK4 pKa = 8.6ATVAMRR10 pKa = 11.84YY11 pKa = 9.59DD12 pKa = 3.56KK13 pKa = 10.84SLSEE17 pKa = 4.52TPPEE21 pKa = 4.59IPTTSQNSEE30 pKa = 3.84SRR32 pKa = 11.84QTSSSIQTEE41 pKa = 4.35TEE43 pKa = 3.72DD44 pKa = 3.7ALISEE49 pKa = 4.87KK50 pKa = 10.63EE51 pKa = 4.18LNSLLGSIPATNQDD65 pKa = 4.48KK66 pKa = 9.91MADD69 pKa = 3.79TNKK72 pKa = 10.65GLIFSGDD79 pKa = 3.91IEE81 pKa = 4.44QCWQGIYY88 pKa = 10.77DD89 pKa = 4.2KK90 pKa = 11.51ASVDD94 pKa = 3.79PKK96 pKa = 10.74HH97 pKa = 6.17LSNYY101 pKa = 7.43EE102 pKa = 3.79LEE104 pKa = 4.28IRR106 pKa = 11.84SLQWGWVHH114 pKa = 6.74CVTDD118 pKa = 4.45APSSLINRR126 pKa = 11.84RR127 pKa = 11.84IDD129 pKa = 3.06SSTEE133 pKa = 3.66ANCRR137 pKa = 11.84AIVAQGKK144 pKa = 7.88GLRR147 pKa = 11.84DD148 pKa = 3.46NMGLKK153 pKa = 9.85EE154 pKa = 4.12ISVPIPAGGKK164 pKa = 9.85GVVPVNNTAKK174 pKa = 10.33IKK176 pKa = 10.35RR177 pKa = 11.84RR178 pKa = 11.84EE179 pKa = 4.21DD180 pKa = 3.37GLSARR185 pKa = 11.84ARR187 pKa = 11.84KK188 pKa = 9.22IADD191 pKa = 3.52DD192 pKa = 4.04LRR194 pKa = 11.84NNPARR199 pKa = 11.84IRR201 pKa = 11.84GFLAHH206 pKa = 6.28GHH208 pKa = 6.49ANRR211 pKa = 11.84KK212 pKa = 5.31WTTAVCRR219 pKa = 11.84YY220 pKa = 9.31LRR222 pKa = 11.84AGKK225 pKa = 10.37GIAPEE230 pKa = 4.25TNEE233 pKa = 3.52ITSTINSGDD242 pKa = 3.05KK243 pKa = 10.0TAGKK247 pKa = 10.05KK248 pKa = 10.31LSSTLNSATKK258 pKa = 10.67LLVDD262 pKa = 3.61AWATKK267 pKa = 10.44DD268 pKa = 3.36VGPIMEE274 pKa = 4.95KK275 pKa = 10.26KK276 pKa = 10.2DD277 pKa = 3.51SKK279 pKa = 11.04EE280 pKa = 3.69DD281 pKa = 3.71AGILDD286 pKa = 4.67DD287 pKa = 6.8DD288 pKa = 5.17IVDD291 pKa = 3.77EE292 pKa = 5.25ANNEE296 pKa = 3.9YY297 pKa = 10.48HH298 pKa = 7.03AGAVYY303 pKa = 10.43ASARR307 pKa = 11.84TAGILSAVHH316 pKa = 5.62NAKK319 pKa = 10.02VCIHH323 pKa = 6.72CLLANTAAPLPTTEE337 pKa = 5.03GRR339 pKa = 11.84FSHH342 pKa = 6.91LNLDD346 pKa = 3.74EE347 pKa = 4.55LRR349 pKa = 11.84NLNLQRR355 pKa = 11.84MLKK358 pKa = 9.69SDD360 pKa = 3.29AVGYY364 pKa = 9.25LQACAAIHH372 pKa = 5.91NRR374 pKa = 11.84EE375 pKa = 3.8QHH377 pKa = 5.76ASNGNIHH384 pKa = 5.78FTAHH388 pKa = 6.08AGMVRR393 pKa = 11.84FDD395 pKa = 3.77VGLMARR401 pKa = 11.84QKK403 pKa = 11.5ADD405 pKa = 3.3TLKK408 pKa = 11.07LVMDD412 pKa = 4.37TEE414 pKa = 4.71RR415 pKa = 11.84PTPAALLNVCEE426 pKa = 4.12NNIAKK431 pKa = 10.68ANFYY435 pKa = 10.05TEE437 pKa = 4.07NSKK440 pKa = 11.03HH441 pKa = 6.02SVLPSAQGTLYY452 pKa = 10.49QYY454 pKa = 10.79TYY456 pKa = 11.46DD457 pKa = 3.69PVIWDD462 pKa = 4.48DD463 pKa = 3.9VLALAKK469 pKa = 8.97NTNVTDD475 pKa = 3.61NNLLDD480 pKa = 4.0GFAEE484 pKa = 3.9IEE486 pKa = 4.1GWKK489 pKa = 10.37KK490 pKa = 10.45VVALGARR497 pKa = 11.84SNCDD501 pKa = 2.8SSDD504 pKa = 3.61EE505 pKa = 4.13YY506 pKa = 11.94SSITLDD512 pKa = 4.92AIIEE516 pKa = 4.2AANEE520 pKa = 4.05QTSLEE525 pKa = 4.35IPHH528 pKa = 6.61HH529 pKa = 6.38LKK531 pKa = 10.07PKK533 pKa = 8.63YY534 pKa = 10.48VSVHH538 pKa = 5.45TLPSHH543 pKa = 7.17RR544 pKa = 11.84ADD546 pKa = 3.78QLGLTAAATEE556 pKa = 5.07RR557 pKa = 11.84IASSSAFASGGIGINDD573 pKa = 3.17QRR575 pKa = 11.84GYY577 pKa = 11.5NIMNYY582 pKa = 9.73VSGANLLLGSALKK595 pKa = 9.78MATTDD600 pKa = 3.4NLDD603 pKa = 3.09WRR605 pKa = 11.84EE606 pKa = 3.97VGIKK610 pKa = 10.19AAILEE615 pKa = 4.37ACRR618 pKa = 11.84EE619 pKa = 4.08PSMTWPTGQDD629 pKa = 2.51WAIPKK634 pKa = 9.91RR635 pKa = 11.84AVPQTRR641 pKa = 11.84YY642 pKa = 8.91WPTVDD647 pKa = 3.22KK648 pKa = 10.73HH649 pKa = 5.97IKK651 pKa = 10.05AVVVSINYY659 pKa = 9.21IDD661 pKa = 3.52ACLRR665 pKa = 11.84EE666 pKa = 4.1NNGLKK671 pKa = 10.53VSVDD675 pKa = 3.35GVIDD679 pKa = 3.45HH680 pKa = 7.27WNLTDD685 pKa = 4.04ANLKK689 pKa = 9.75VVSLSEE695 pKa = 4.11DD696 pKa = 3.64TNTTEE701 pKa = 4.14LTRR704 pKa = 11.84SLRR707 pKa = 11.84TILALPFPVVNVIDD721 pKa = 4.49EE722 pKa = 4.26YY723 pKa = 10.49QIKK726 pKa = 10.53YY727 pKa = 10.57LGDD730 pKa = 3.44KK731 pKa = 10.61SDD733 pKa = 3.52VYY735 pKa = 11.11SRR737 pKa = 11.84KK738 pKa = 9.65CFYY741 pKa = 11.05RR742 pKa = 11.84NASLVAMGDD751 pKa = 3.25KK752 pKa = 10.33VDD754 pKa = 4.77RR755 pKa = 11.84IVFIVPDD762 pKa = 3.86KK763 pKa = 10.75SQTVVKK769 pKa = 10.63LGGVYY774 pKa = 10.17RR775 pKa = 11.84KK776 pKa = 9.05VACPTRR782 pKa = 11.84FNAIEE787 pKa = 4.28SVPDD791 pKa = 2.76VWPAHH796 pKa = 4.64EE797 pKa = 4.79TILRR801 pKa = 11.84MIHH804 pKa = 5.74TLMGCDD810 pKa = 2.76RR811 pKa = 11.84NLRR814 pKa = 11.84VVCNEE819 pKa = 3.33YY820 pKa = 9.18MRR822 pKa = 11.84KK823 pKa = 9.75YY824 pKa = 10.92SEE826 pKa = 6.02APLNWNEE833 pKa = 3.74VQAICGALITRR844 pKa = 11.84FPRR847 pKa = 11.84QPHH850 pKa = 5.46VYY852 pKa = 9.22ISYY855 pKa = 10.59VNEE858 pKa = 4.1DD859 pKa = 3.72DD860 pKa = 4.39EE861 pKa = 6.18APEE864 pKa = 3.96HH865 pKa = 6.1QYY867 pKa = 11.49SYY869 pKa = 11.59GLPKK873 pKa = 10.56KK874 pKa = 9.51MLSAYY879 pKa = 8.82GLRR882 pKa = 11.84GFTPQDD888 pKa = 3.19EE889 pKa = 4.21NDD891 pKa = 3.13ARR893 pKa = 11.84FFGHH897 pKa = 6.64EE898 pKa = 3.96EE899 pKa = 3.75AVTCFSQVLIDD910 pKa = 4.77TLQQCQDD917 pKa = 3.36TCHH920 pKa = 6.93LSLGRR925 pKa = 11.84WSNYY929 pKa = 10.22AEE931 pKa = 4.51LLMAASVVTLIPGTALGSDD950 pKa = 3.43NTKK953 pKa = 10.79LGLCATGLDD962 pKa = 3.93TLNKK966 pKa = 8.98ISLYY970 pKa = 10.42RR971 pKa = 11.84RR972 pKa = 11.84GTEE975 pKa = 3.65EE976 pKa = 3.67WASASGMSEE985 pKa = 3.87YY986 pKa = 11.12VLNPRR991 pKa = 11.84LYY993 pKa = 10.65SRR995 pKa = 11.84SAGNIEE1001 pKa = 4.18AYY1003 pKa = 8.42ITGGGNDD1010 pKa = 3.39SRR1012 pKa = 11.84IVTLQVWASTCIGGASLSWYY1032 pKa = 8.66WSSNYY1037 pKa = 9.43EE1038 pKa = 3.86KK1039 pKa = 10.54SVPLAPNHH1047 pKa = 6.12RR1048 pKa = 11.84RR1049 pKa = 11.84LGSGLYY1055 pKa = 8.06QTDD1058 pKa = 3.42YY1059 pKa = 10.4TLCLRR1064 pKa = 11.84GLDD1067 pKa = 3.63LPPRR1071 pKa = 11.84NFVSNTTIGSRR1082 pKa = 11.84GYY1084 pKa = 10.46GWAHH1088 pKa = 6.02LRR1090 pKa = 11.84GLEE1093 pKa = 4.04PVDD1096 pKa = 4.31DD1097 pKa = 4.15YY1098 pKa = 11.65EE1099 pKa = 4.33IEE1101 pKa = 4.31KK1102 pKa = 10.5FVSRR1106 pKa = 11.84LDD1108 pKa = 3.52FHH1110 pKa = 7.46KK1111 pKa = 11.03VNEE1114 pKa = 4.07WGYY1117 pKa = 9.49LQWPDD1122 pKa = 3.53GNIKK1126 pKa = 10.45RR1127 pKa = 11.84EE1128 pKa = 3.46GSYY1131 pKa = 10.29RR1132 pKa = 11.84VTSTTTAGLASLAGWRR1148 pKa = 11.84GFNDD1152 pKa = 3.18SYY1154 pKa = 11.1KK1155 pKa = 10.76GVWGWGVDD1163 pKa = 3.82YY1164 pKa = 10.17MKK1166 pKa = 10.59TDD1168 pKa = 3.85LKK1170 pKa = 10.84FEE1172 pKa = 4.26TMATLALRR1180 pKa = 11.84PTTLSRR1186 pKa = 11.84VSASVFVPGLWQSIGIGLDD1205 pKa = 3.15ASVEE1209 pKa = 4.59TTGSMIDD1216 pKa = 3.31RR1217 pKa = 11.84MRR1219 pKa = 11.84GFSLAAQPPDD1229 pKa = 3.42GSFLSQLEE1237 pKa = 4.32RR1238 pKa = 11.84VLSLHH1243 pKa = 6.61LPTRR1247 pKa = 11.84SDD1249 pKa = 3.23GVTSTTSMAQLVEE1262 pKa = 3.99QANRR1266 pKa = 11.84TEE1268 pKa = 4.01AASIEE1273 pKa = 4.5SFHH1276 pKa = 6.54GAIPSPLPSSNPEE1289 pKa = 4.13VIPKK1293 pKa = 9.34TGTSQNSTPQSMEE1306 pKa = 3.71DD1307 pKa = 2.89QVMINRR1313 pKa = 11.84AIPPTHH1319 pKa = 7.37KK1320 pKa = 10.28IGTDD1324 pKa = 3.3GAGGVTLDD1332 pKa = 3.26QVARR1336 pKa = 11.84TDD1338 pKa = 3.5VNN1340 pKa = 3.38

Molecular weight:
147.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFE7|A0A1L3KFE7_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 21 OX=1923310 PE=4 SV=1
MM1 pKa = 7.37MSNRR5 pKa = 11.84WPGLCTCKK13 pKa = 10.04IKK15 pKa = 10.69KK16 pKa = 9.23ILKK19 pKa = 9.46KK20 pKa = 9.43QTATAKK26 pKa = 10.82LGGKK30 pKa = 9.93YY31 pKa = 9.18IDD33 pKa = 3.65EE34 pKa = 4.53GWRR37 pKa = 11.84NLCYY41 pKa = 9.87WEE43 pKa = 4.22TFYY46 pKa = 11.27GYY48 pKa = 10.69LPYY51 pKa = 10.61KK52 pKa = 10.34GVEE55 pKa = 3.87ALKK58 pKa = 10.92EE59 pKa = 4.21EE60 pKa = 4.47VEE62 pKa = 4.31TWLVRR67 pKa = 11.84PIRR70 pKa = 11.84LGGPLSEE77 pKa = 4.51EE78 pKa = 5.01EE79 pKa = 4.24YY80 pKa = 11.1LSMLSEE86 pKa = 4.2EE87 pKa = 4.56MVKK90 pKa = 10.75LMDD93 pKa = 4.88EE94 pKa = 4.43EE95 pKa = 4.63WTMPPHH101 pKa = 5.56TLEE104 pKa = 4.47PEE106 pKa = 3.98QWVSTGRR113 pKa = 11.84WMRR116 pKa = 11.84GRR118 pKa = 11.84SGTGPATTVEE128 pKa = 4.24INQKK132 pKa = 7.2ITRR135 pKa = 11.84TRR137 pKa = 11.84RR138 pKa = 11.84MKK140 pKa = 10.9GVDD143 pKa = 3.37ASLLSDD149 pKa = 3.3KK150 pKa = 11.6DD151 pKa = 3.5MVEE154 pKa = 4.08QLSHH158 pKa = 5.75VVQEE162 pKa = 4.63TFHH165 pKa = 8.16IMEE168 pKa = 4.66KK169 pKa = 10.51SEE171 pKa = 3.93GGKK174 pKa = 9.34IRR176 pKa = 11.84PIVKK180 pKa = 8.89TGSAMFRR187 pKa = 11.84KK188 pKa = 9.0MDD190 pKa = 4.7YY191 pKa = 10.67LSQWCEE197 pKa = 3.74DD198 pKa = 3.54GFKK201 pKa = 10.43HH202 pKa = 6.7SKK204 pKa = 10.47LSTLFGSASNQEE216 pKa = 4.21WIDD219 pKa = 3.64RR220 pKa = 11.84DD221 pKa = 3.45ILEE224 pKa = 4.42SAKK227 pKa = 10.74NPNLWKK233 pKa = 10.91VPMDD237 pKa = 3.3QSNFDD242 pKa = 3.27WHH244 pKa = 5.61QSKK247 pKa = 10.92ASIMTIIATMGIYY260 pKa = 9.92IGQRR264 pKa = 11.84TSAGGFHH271 pKa = 5.72QVWNAMWDD279 pKa = 3.7SIFAQQVTVHH289 pKa = 6.63CGRR292 pKa = 11.84YY293 pKa = 9.06KK294 pKa = 10.61YY295 pKa = 10.37SWEE298 pKa = 3.76NGMPSGFRR306 pKa = 11.84WTALIDD312 pKa = 3.4TLLNITSFRR321 pKa = 11.84VAVRR325 pKa = 11.84IASEE329 pKa = 4.12TKK331 pKa = 9.49KK332 pKa = 10.59QYY334 pKa = 10.6IHH336 pKa = 6.92IAHH339 pKa = 6.77HH340 pKa = 6.77RR341 pKa = 11.84SQGDD345 pKa = 3.38DD346 pKa = 3.15VAFATRR352 pKa = 11.84EE353 pKa = 4.01LSDD356 pKa = 3.33VALIMHH362 pKa = 7.42IYY364 pKa = 10.06NRR366 pKa = 11.84IGYY369 pKa = 7.36EE370 pKa = 3.71AHH372 pKa = 6.6PSKK375 pKa = 9.99TFFSRR380 pKa = 11.84YY381 pKa = 5.55RR382 pKa = 11.84TEE384 pKa = 4.33FLRR387 pKa = 11.84KK388 pKa = 9.62SYY390 pKa = 10.46EE391 pKa = 3.83AGLGITGYY399 pKa = 10.09LARR402 pKa = 11.84SLLSIRR408 pKa = 11.84FRR410 pKa = 11.84SPILEE415 pKa = 4.11MPIVRR420 pKa = 11.84ATRR423 pKa = 11.84LYY425 pKa = 10.83SRR427 pKa = 11.84LTGWHH432 pKa = 6.5LMTLRR437 pKa = 11.84GATPKK442 pKa = 10.58AAATCFLWDD451 pKa = 3.77AEE453 pKa = 4.16QLGVDD458 pKa = 3.6RR459 pKa = 11.84TTAANFAMTPSSFGGAGLWPDD480 pKa = 3.74GLMSTYY486 pKa = 10.64LKK488 pKa = 10.55PHH490 pKa = 6.07FTHH493 pKa = 6.5FTTYY497 pKa = 10.52EE498 pKa = 3.83IVTEE502 pKa = 3.88YY503 pKa = 11.1HH504 pKa = 6.93KK505 pKa = 10.59IQPRR509 pKa = 11.84LGHH512 pKa = 5.4WNEE515 pKa = 3.86RR516 pKa = 11.84LKK518 pKa = 11.27SLGSYY523 pKa = 9.87LGPRR527 pKa = 11.84EE528 pKa = 3.75INAFNITLAKK538 pKa = 9.63SWGIRR543 pKa = 11.84DD544 pKa = 3.13VDD546 pKa = 3.66AVKK549 pKa = 10.13EE550 pKa = 4.02ARR552 pKa = 11.84VEE554 pKa = 4.04WKK556 pKa = 9.93EE557 pKa = 3.62CQARR561 pKa = 11.84IRR563 pKa = 11.84RR564 pKa = 11.84PTSWPAPLHH573 pKa = 6.6HH574 pKa = 6.86YY575 pKa = 10.43ADD577 pKa = 3.76YY578 pKa = 10.66WNLEE582 pKa = 4.24GIPVLIRR589 pKa = 11.84PYY591 pKa = 10.62VITGALDD598 pKa = 2.99KK599 pKa = 10.64GTWTKK604 pKa = 10.2LAKK607 pKa = 10.12PEE609 pKa = 4.53MIPQIAAYY617 pKa = 8.8IRR619 pKa = 11.84RR620 pKa = 11.84VSKK623 pKa = 10.8NVARR627 pKa = 11.84AYY629 pKa = 10.87LSGQISLPWPMLDD642 pKa = 4.82GIAMKK647 pKa = 10.62YY648 pKa = 9.91GNTVRR653 pKa = 11.84KK654 pKa = 9.44VLDD657 pKa = 3.11MRR659 pKa = 11.84LRR661 pKa = 11.84QICNIKK667 pKa = 10.64DD668 pKa = 3.18LGLRR672 pKa = 11.84EE673 pKa = 4.05LSEE676 pKa = 3.94FAGWLEE682 pKa = 3.89RR683 pKa = 11.84RR684 pKa = 11.84AGALLYY690 pKa = 10.24QEE692 pKa = 5.05YY693 pKa = 10.8SDD695 pKa = 5.02GVYY698 pKa = 9.23ATT700 pKa = 4.43

Molecular weight:
80.35 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

2040

700

1340

1020.0

113.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.382 ± 0.586

1.471 ± 0.175

5.245 ± 0.742

5.539 ± 0.399

2.353 ± 0.422

7.108 ± 0.095

2.451 ± 0.141

5.882 ± 0.292

5.098 ± 0.406

8.971 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.549 ± 0.623

4.608 ± 1.012

4.363 ± 0.041

3.088 ± 0.029

6.078 ± 0.569

7.745 ± 0.551

7.059 ± 0.108

5.833 ± 0.675

2.549 ± 0.623

3.627 ± 0.352

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski