Halovenus sp. WSH3

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halovenus; unclassified Halovenus

Average proteome isoelectric point is 4.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B0T6E9|A0A6B0T6E9_9EURY Winged helix-turn-helix transcriptional regulator OS=Halovenus sp. WSH3 OX=2692199 GN=GRX03_09135 PE=4 SV=1
MM1 pKa = 6.48TAEE4 pKa = 4.91RR5 pKa = 11.84SPEE8 pKa = 3.93PDD10 pKa = 3.22PQVEE14 pKa = 4.24QFLQLYY20 pKa = 10.62DD21 pKa = 3.89SMDD24 pKa = 3.44VPEE27 pKa = 4.63LHH29 pKa = 6.74EE30 pKa = 4.69LSPPEE35 pKa = 3.78ARR37 pKa = 11.84EE38 pKa = 3.86VQEE41 pKa = 4.53QLSQADD47 pKa = 3.94PDD49 pKa = 3.62IDD51 pKa = 4.08LPTVEE56 pKa = 5.73DD57 pKa = 3.83RR58 pKa = 11.84TIDD61 pKa = 3.94GPDD64 pKa = 3.18GEE66 pKa = 4.56IPIRR70 pKa = 11.84LYY72 pKa = 10.83DD73 pKa = 3.69PRR75 pKa = 11.84TEE77 pKa = 4.08EE78 pKa = 4.21TEE80 pKa = 4.04TPLILFYY87 pKa = 10.87HH88 pKa = 6.6GGGFVVGSLDD98 pKa = 3.58THH100 pKa = 7.33DD101 pKa = 4.41GPCRR105 pKa = 11.84KK106 pKa = 9.21LAAEE110 pKa = 3.91TGYY113 pKa = 10.07PVVSVDD119 pKa = 3.24YY120 pKa = 10.49RR121 pKa = 11.84LAPEE125 pKa = 4.58HH126 pKa = 6.76PFPAGLNDD134 pKa = 4.41CYY136 pKa = 11.2AALEE140 pKa = 4.37WASEE144 pKa = 3.95TDD146 pKa = 3.19EE147 pKa = 5.37LGIDD151 pKa = 3.37SGQIVVAGDD160 pKa = 3.48SAGGNFAAAVSLLSRR175 pKa = 11.84DD176 pKa = 3.22RR177 pKa = 11.84DD178 pKa = 3.85GPEE181 pKa = 3.12IAAQVLVYY189 pKa = 10.07PVTGDD194 pKa = 3.31AAEE197 pKa = 4.24TDD199 pKa = 4.17SYY201 pKa = 11.21DD202 pKa = 3.82QNGEE206 pKa = 4.25GYY208 pKa = 10.43FLTTEE213 pKa = 4.01TMEE216 pKa = 4.3WFHH219 pKa = 6.98DD220 pKa = 3.84QYY222 pKa = 11.51LDD224 pKa = 4.52DD225 pKa = 6.43PIDD228 pKa = 3.66EE229 pKa = 4.54GNIYY233 pKa = 9.7VAPRR237 pKa = 11.84LAADD241 pKa = 4.89LSDD244 pKa = 4.79LPPATVVTAGYY255 pKa = 10.56DD256 pKa = 3.49PLRR259 pKa = 11.84DD260 pKa = 3.96DD261 pKa = 3.97GALYY265 pKa = 10.82AEE267 pKa = 4.74RR268 pKa = 11.84LRR270 pKa = 11.84DD271 pKa = 3.48AGVEE275 pKa = 4.24VSHH278 pKa = 7.0HH279 pKa = 5.94NFGGMIHH286 pKa = 6.93GFFNMIADD294 pKa = 4.2PVDD297 pKa = 2.91IDD299 pKa = 4.18AAHH302 pKa = 6.64EE303 pKa = 4.4AYY305 pKa = 10.38DD306 pKa = 4.51AVVADD311 pKa = 5.05LDD313 pKa = 4.89GMLDD317 pKa = 3.65DD318 pKa = 5.06

Molecular weight: 34.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B0T7Z4|A0A6B0T7Z4_9EURY Uncharacterized protein OS=Halovenus sp. WSH3 OX=2692199 GN=GRX03_12020 PE=4 SV=1
MM1 pKa = 7.26TPRR4 pKa = 11.84SRR6 pKa = 11.84ALGVDD11 pKa = 4.57FGLGAGAVGLGALAGGTPQSLDD33 pKa = 3.0GFLIGGIGLFAFVATLYY50 pKa = 10.52VAEE53 pKa = 4.09QTKK56 pKa = 10.5VLVLIEE62 pKa = 4.1RR63 pKa = 11.84TRR65 pKa = 11.84PTSLVVACVTLAAVGVALVVGQVVVEE91 pKa = 4.85TPAATLLVGMGTGLIGYY108 pKa = 7.19RR109 pKa = 11.84LWFGLVAPVPPKK121 pKa = 10.63RR122 pKa = 11.84LDD124 pKa = 3.33QASVWGSRR132 pKa = 11.84RR133 pKa = 11.84NRR135 pKa = 11.84KK136 pKa = 8.63

Molecular weight: 13.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3185

0

3185

956425

41

2990

300.3

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.178 ± 0.054

0.734 ± 0.013

8.085 ± 0.043

9.47 ± 0.064

3.232 ± 0.029

8.492 ± 0.045

1.905 ± 0.022

4.527 ± 0.032

1.808 ± 0.027

8.892 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.722 ± 0.019

2.37 ± 0.026

4.631 ± 0.028

2.977 ± 0.023

6.561 ± 0.046

5.657 ± 0.035

6.481 ± 0.04

8.512 ± 0.043

1.06 ± 0.018

2.707 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski