Cyanophage P-SSP2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Tritonvirus; Synechococcus virus PSSP2

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3SQM6|E3SQM6_9CAUD Hgh-light inducible protein OS=Cyanophage P-SSP2 OX=444876 GN=CYLG_00044 PE=4 SV=1
MM1 pKa = 7.98ADD3 pKa = 3.37TLTIKK8 pKa = 10.44QDD10 pKa = 3.62DD11 pKa = 3.67QSTDD15 pKa = 3.3VEE17 pKa = 4.31NLTTEE22 pKa = 4.34EE23 pKa = 3.9QDD25 pKa = 3.43SLQVGEE31 pKa = 4.92EE32 pKa = 3.88MAKK35 pKa = 10.22EE36 pKa = 3.9QGEE39 pKa = 4.28LLAGKK44 pKa = 9.56YY45 pKa = 10.12KK46 pKa = 10.6NAEE49 pKa = 4.36DD50 pKa = 3.86LEE52 pKa = 4.31KK53 pKa = 10.72AYY55 pKa = 11.21VEE57 pKa = 4.11LQKK60 pKa = 11.41KK61 pKa = 10.28LGDD64 pKa = 3.75KK65 pKa = 10.56EE66 pKa = 4.25EE67 pKa = 4.55PEE69 pKa = 3.91ATKK72 pKa = 10.8DD73 pKa = 3.53EE74 pKa = 4.53EE75 pKa = 4.52EE76 pKa = 4.31VTDD79 pKa = 3.83TKK81 pKa = 11.33DD82 pKa = 3.23EE83 pKa = 4.4PEE85 pKa = 4.42EE86 pKa = 4.28KK87 pKa = 10.89GEE89 pKa = 4.11AYY91 pKa = 10.7SLIEE95 pKa = 4.24SASDD99 pKa = 3.24EE100 pKa = 4.41YY101 pKa = 10.67YY102 pKa = 11.02QNGEE106 pKa = 4.2SLSPEE111 pKa = 3.9TLEE114 pKa = 4.32KK115 pKa = 10.4FKK117 pKa = 11.03GMSSQDD123 pKa = 3.41LVEE126 pKa = 6.49GYY128 pKa = 8.33MQMVKK133 pKa = 10.72DD134 pKa = 4.24NPQTNQPEE142 pKa = 4.26IDD144 pKa = 3.66VTTAEE149 pKa = 4.13INKK152 pKa = 8.9IQNSVGGEE160 pKa = 3.94TQYY163 pKa = 11.98NNLVSWAGQNLPEE176 pKa = 4.15NEE178 pKa = 3.64IKK180 pKa = 10.95AFDD183 pKa = 4.34DD184 pKa = 4.68LVGTGNAAAIQLGVDD199 pKa = 3.58ALKK202 pKa = 10.79SRR204 pKa = 11.84YY205 pKa = 8.49EE206 pKa = 3.85AVNGYY211 pKa = 8.25EE212 pKa = 4.1GRR214 pKa = 11.84RR215 pKa = 11.84LTGKK219 pKa = 10.26AADD222 pKa = 3.61TSGDD226 pKa = 3.52VFRR229 pKa = 11.84SQAQLVEE236 pKa = 4.33AMSDD240 pKa = 3.21PRR242 pKa = 11.84YY243 pKa = 10.61DD244 pKa = 3.27RR245 pKa = 11.84DD246 pKa = 2.99PAYY249 pKa = 10.68RR250 pKa = 11.84QDD252 pKa = 3.44VVAKK256 pKa = 9.85LEE258 pKa = 4.06RR259 pKa = 11.84SDD261 pKa = 3.91IDD263 pKa = 3.57FF264 pKa = 4.63

Molecular weight: 29.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3SQJ3|E3SQJ3_9CAUD HNHc domain-containing protein OS=Cyanophage P-SSP2 OX=444876 GN=CYLG_00008 PE=4 SV=1
MM1 pKa = 7.5PSKK4 pKa = 10.31GAKK7 pKa = 8.26YY8 pKa = 10.91ANGNYY13 pKa = 9.57KK14 pKa = 10.17AQQKK18 pKa = 10.49AYY20 pKa = 9.74NKK22 pKa = 8.26TKK24 pKa = 10.06KK25 pKa = 10.09GLKK28 pKa = 9.5IRR30 pKa = 11.84VNANRR35 pKa = 11.84LNRR38 pKa = 11.84QLGTYY43 pKa = 10.5GNGDD47 pKa = 3.71GKK49 pKa = 10.59DD50 pKa = 3.15AAHH53 pKa = 6.38YY54 pKa = 9.83KK55 pKa = 10.61GSSTKK60 pKa = 10.25GRR62 pKa = 11.84LQSPSKK68 pKa = 10.3NRR70 pKa = 11.84KK71 pKa = 8.58SRR73 pKa = 11.84LKK75 pKa = 10.06IRR77 pKa = 11.84KK78 pKa = 8.67

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13209

46

1354

235.9

26.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.085 ± 0.317

0.946 ± 0.167

6.745 ± 0.343

6.215 ± 0.401

3.778 ± 0.21

6.889 ± 0.356

1.628 ± 0.183

5.928 ± 0.275

6.798 ± 0.435

8.085 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.24

5.504 ± 0.352

3.876 ± 0.311

4.164 ± 0.309

4.436 ± 0.312

6.761 ± 0.369

7.116 ± 0.501

6.094 ± 0.248

1.166 ± 0.136

3.535 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski