Acinetobacter populi
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3291 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z9YYX0|A0A1Z9YYX0_9GAMM Uncharacterized protein OS=Acinetobacter populi OX=1582270 GN=CAP51_06270 PE=4 SV=1
MM1 pKa = 7.84 DD2 pKa = 4.11 TTPIISGSTGFDD14 pKa = 2.99 ILEE17 pKa = 4.27 GEE19 pKa = 4.34 YY20 pKa = 11.24 LEE22 pKa = 4.26 VTVNSKK28 pKa = 9.26 TYY30 pKa = 10.38 SSQTGAVVIDD40 pKa = 3.98 FDD42 pKa = 4.39 NNTWYY47 pKa = 10.77 VQIPDD52 pKa = 4.03 SDD54 pKa = 4.04 ALPIGTYY61 pKa = 9.95 NVSAVLKK68 pKa = 9.35 NAEE71 pKa = 4.14 GEE73 pKa = 4.2 ITQDD77 pKa = 3.09 DD78 pKa = 4.33 TTNEE82 pKa = 4.18 LVVSPSPTINFTATAATSDD101 pKa = 3.77 DD102 pKa = 3.71 TGTALTISEE111 pKa = 4.14 NGTWRR116 pKa = 11.84 ILSNSTVFTQNATDD130 pKa = 4.08 PSTLGSFTSIAISGADD146 pKa = 3.43 RR147 pKa = 11.84 QQQSSFIDD155 pKa = 3.85 FDD157 pKa = 4.65 RR158 pKa = 11.84 DD159 pKa = 3.11 GLMDD163 pKa = 4.12 ILGADD168 pKa = 3.3 TSFANGQQSFKK179 pKa = 10.96 YY180 pKa = 9.88 NADD183 pKa = 2.79 GTYY186 pKa = 9.78 TVFQIGSFGISGQTNDD202 pKa = 4.22 ANGNTYY208 pKa = 8.78 VWYY211 pKa = 10.42 GGVAGIDD218 pKa = 3.49 INGDD222 pKa = 3.54 GYY224 pKa = 11.75 VDD226 pKa = 3.34 IVYY229 pKa = 10.74 GDD231 pKa = 3.79 EE232 pKa = 4.35 TPNDD236 pKa = 3.38 AEE238 pKa = 4.35 TRR240 pKa = 11.84 GGYY243 pKa = 7.68 DD244 pKa = 2.84 TTFVLNTNGTILGFNKK260 pKa = 10.17 SGAYY264 pKa = 10.1 VYY266 pKa = 9.29 TQTNQDD272 pKa = 2.74 GVAATNSGNPTPDD285 pKa = 3.18 RR286 pKa = 11.84 EE287 pKa = 4.32 IAGVDD292 pKa = 3.94 LNNDD296 pKa = 3.48 GYY298 pKa = 11.86 VDD300 pKa = 3.69 IVYY303 pKa = 10.31 HH304 pKa = 5.67 GTAGTNTTSTGGSSGVSTRR323 pKa = 11.84 LVVVNNGVDD332 pKa = 3.51 ANGNTTLTNTQIVTDD347 pKa = 4.19 VFNGDD352 pKa = 3.26 NGTTNVYY359 pKa = 9.97 TSLTWADD366 pKa = 3.97 FNGDD370 pKa = 3.01 GYY372 pKa = 10.9 MDD374 pKa = 4.62 LFIGGLTGTGTAANSEE390 pKa = 4.24 IYY392 pKa = 11.04 YY393 pKa = 10.62 NDD395 pKa = 3.44 GTGKK399 pKa = 10.67 LVTTTNGVGTGTNVQTLTDD418 pKa = 3.7 ATNSNTSLAVDD429 pKa = 3.48 WNGDD433 pKa = 3.3 GKK435 pKa = 10.66 IDD437 pKa = 3.81 IIEE440 pKa = 4.13 IAGISQGNSAANVSSADD457 pKa = 3.36 NKK459 pKa = 10.8 GILWLNGGTNASGQVNWTSEE479 pKa = 4.37 TILAQANIRR488 pKa = 11.84 PGAASTYY495 pKa = 9.49 RR496 pKa = 11.84 FVSGAQVVDD505 pKa = 4.2 LDD507 pKa = 4.02 YY508 pKa = 11.67 DD509 pKa = 4.08 GDD511 pKa = 3.62 QDD513 pKa = 4.31 LVVFRR518 pKa = 11.84 SEE520 pKa = 4.23 AGATSYY526 pKa = 10.94 IEE528 pKa = 4.15 NKK530 pKa = 10.38 SVIQDD535 pKa = 3.4 GTSIILNIRR544 pKa = 11.84 DD545 pKa = 3.59 KK546 pKa = 11.61 NGINAYY552 pKa = 9.83 YY553 pKa = 10.05 GNTVALIDD561 pKa = 3.88 EE562 pKa = 4.56 ATGQVVATQIINAQSGVNTQNSTGLVYY589 pKa = 9.84 FYY591 pKa = 11.28 GLDD594 pKa = 3.35 ASKK597 pKa = 10.75 SYY599 pKa = 11.09 SAVILSNGNDD609 pKa = 3.36 YY610 pKa = 11.62 GGISSITLGSSVNTIEE626 pKa = 4.93 NVNEE630 pKa = 3.66 TWAGLKK636 pKa = 10.24 AVEE639 pKa = 4.42 KK640 pKa = 9.59 NHH642 pKa = 6.82 AYY644 pKa = 10.75 VLTTEE649 pKa = 4.29 NGTAASSTATAATDD663 pKa = 3.44 GTNTVGILGTGYY675 pKa = 11.0 NDD677 pKa = 3.4 TLYY680 pKa = 9.94 ATAGTHH686 pKa = 5.74 VYY688 pKa = 10.29 NGAGGSEE695 pKa = 4.26 LVSGVNTWSDD705 pKa = 3.26 TGGMDD710 pKa = 3.62 IVDD713 pKa = 3.79 YY714 pKa = 10.95 KK715 pKa = 11.13 LAGSTAITVDD725 pKa = 3.97 LSITTAQNTGFGTATFVNIEE745 pKa = 4.34 GIAGSSSNDD754 pKa = 3.12 TFTDD758 pKa = 3.31 NAGNNQFEE766 pKa = 4.43 GRR768 pKa = 11.84 GGNDD772 pKa = 2.48 IFNLINGGNDD782 pKa = 2.89 TLLYY786 pKa = 10.35 KK787 pKa = 10.79 VLDD790 pKa = 4.0 AANATGGNGSDD801 pKa = 3.6 VVNGFHH807 pKa = 6.7 VGTWEE812 pKa = 3.86 ATPNADD818 pKa = 5.61 RR819 pKa = 11.84 IDD821 pKa = 4.0 LSEE824 pKa = 4.37 LLIGYY829 pKa = 6.79 TGSVTPASYY838 pKa = 11.05 INGTPTLAADD848 pKa = 3.62 AEE850 pKa = 4.2 IRR852 pKa = 11.84 NYY854 pKa = 11.05 LSVQSDD860 pKa = 3.75 GTNTTISIDD869 pKa = 3.43 RR870 pKa = 11.84 DD871 pKa = 3.84 GAGGAYY877 pKa = 10.3 SSTTLVTLNDD887 pKa = 3.02 VDD889 pKa = 3.98 TTLEE893 pKa = 3.95 KK894 pKa = 10.97 LLANHH899 pKa = 6.45 QIIIGG904 pKa = 3.69
Molecular weight: 93.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.528
Grimsley 3.376
Solomon 3.719
Lehninger 3.668
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.834
Patrickios 0.642
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.743
Protein with the highest isoelectric point:
>tr|A0A1Z9YVZ0|A0A1Z9YVZ0_9GAMM GNAT family N-acetyltransferase OS=Acinetobacter populi OX=1582270 GN=CAP51_14005 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3291
0
3291
1064362
33
3790
323.4
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.668 ± 0.047
0.924 ± 0.014
5.368 ± 0.031
5.514 ± 0.039
4.211 ± 0.035
6.574 ± 0.05
2.418 ± 0.032
7.145 ± 0.037
5.292 ± 0.036
10.442 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.297 ± 0.024
4.505 ± 0.047
3.889 ± 0.029
5.952 ± 0.053
4.253 ± 0.034
6.096 ± 0.042
5.417 ± 0.048
6.379 ± 0.036
1.27 ± 0.018
3.387 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here