Acidianus bottle-shaped virus 2

Taxonomy: Viruses; Ampullaviridae; Bottigliavirus; Bottigliavirus ABV2

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9P4E9|A0A0N9P4E9_9VIRU DNA-directed DNA polymerase OS=Acidianus bottle-shaped virus 2 OX=1732173 PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 3.72KK3 pKa = 10.33TLINHH8 pKa = 6.73LEE10 pKa = 4.09VSTSNLNSVSYY21 pKa = 10.98SVFSTIRR28 pKa = 11.84SLIEE32 pKa = 3.64SGRR35 pKa = 11.84TLNGEE40 pKa = 4.26EE41 pKa = 4.24LTLLLKK47 pKa = 10.59SFKK50 pKa = 10.82SQFTVTLDD58 pKa = 3.27DD59 pKa = 3.73LQYY62 pKa = 11.05VYY64 pKa = 10.65HH65 pKa = 6.61EE66 pKa = 5.24CEE68 pKa = 4.22VSDD71 pKa = 5.21PIYY74 pKa = 10.34PYY76 pKa = 11.04LNYY79 pKa = 10.34YY80 pKa = 10.92DD81 pKa = 5.62DD82 pKa = 4.68VLNSAYY88 pKa = 10.73SSLDD92 pKa = 3.12EE93 pKa = 5.26LIYY96 pKa = 10.76LIEE99 pKa = 4.99KK100 pKa = 9.81EE101 pKa = 3.61FDD103 pKa = 3.37YY104 pKa = 11.03NYY106 pKa = 10.17PSLEE110 pKa = 3.95MLIKK114 pKa = 9.87STLSSIQDD122 pKa = 3.1VIYY125 pKa = 9.89FVHH128 pKa = 6.35EE129 pKa = 4.38WFAKK133 pKa = 10.43LRR135 pKa = 11.84DD136 pKa = 4.01NNEE139 pKa = 3.8LTNTPEE145 pKa = 4.44EE146 pKa = 4.41PNDD149 pKa = 3.68NNNEE153 pKa = 3.99NNNEE157 pKa = 3.98KK158 pKa = 10.51EE159 pKa = 4.43SNDD162 pKa = 3.63EE163 pKa = 3.74NDD165 pKa = 3.47QEE167 pKa = 4.27NTEE170 pKa = 4.53DD171 pKa = 3.56EE172 pKa = 4.58STNNEE177 pKa = 4.05DD178 pKa = 3.12NDD180 pKa = 4.01YY181 pKa = 11.2PKK183 pKa = 10.8DD184 pKa = 3.79EE185 pKa = 5.07PDD187 pKa = 3.11EE188 pKa = 4.84DD189 pKa = 5.2FYY191 pKa = 11.23PQEE194 pKa = 4.12KK195 pKa = 10.38

Molecular weight: 22.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9NJE8|A0A0N9NJE8_9VIRU Uncharacterized protein OS=Acidianus bottle-shaped virus 2 OX=1732173 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 4.27VVYY5 pKa = 10.51LILIVSGVVIGNEE18 pKa = 3.63LVIRR22 pKa = 11.84ILRR25 pKa = 11.84SHH27 pKa = 6.55SLSIHH32 pKa = 5.7KK33 pKa = 8.86MVNRR37 pKa = 11.84NHH39 pKa = 5.86KK40 pKa = 9.87QKK42 pKa = 10.88NKK44 pKa = 9.56QNHH47 pKa = 5.02KK48 pKa = 10.56VITSNKK54 pKa = 7.98EE55 pKa = 3.67RR56 pKa = 11.84NN57 pKa = 3.41

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

6787

37

655

128.1

14.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.7 ± 0.354

1.017 ± 0.275

5.334 ± 0.337

6.689 ± 0.461

5.569 ± 0.327

4.744 ± 0.369

1.09 ± 0.138

9.238 ± 0.486

7.514 ± 0.585

10.314 ± 0.411

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.215

5.835 ± 0.401

3.831 ± 0.357

3.256 ± 0.255

3.3 ± 0.319

7.146 ± 0.564

5.378 ± 0.278

6.483 ± 0.333

0.619 ± 0.081

5.525 ± 0.337
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski