Beihai sobemo-like virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEE8|A0A1L3KEE8_9VIRU Putative capsid protein OS=Beihai sobemo-like virus 8 OX=1922705 PE=4 SV=1
MM1 pKa = 7.75AFTVTISDD9 pKa = 3.89EE10 pKa = 4.64LIEE13 pKa = 4.14SAIWSFFGGGAMFLALKK30 pKa = 10.33IGVFGMLWEE39 pKa = 4.3AASWLLAWVWFVVQVVARR57 pKa = 11.84RR58 pKa = 11.84LAFQLQLGALPVAACKK74 pKa = 9.92TGFIIVGEE82 pKa = 4.01KK83 pKa = 10.07AYY85 pKa = 11.0YY86 pKa = 9.64RR87 pKa = 11.84DD88 pKa = 3.84PVDD91 pKa = 3.34LRR93 pKa = 11.84MYY95 pKa = 10.68SVVPHH100 pKa = 6.81TATCTDD106 pKa = 2.74GKK108 pKa = 10.15MVLKK112 pKa = 10.38DD113 pKa = 3.59YY114 pKa = 11.11RR115 pKa = 11.84LVPLGGDD122 pKa = 3.28AKK124 pKa = 10.56VAEE127 pKa = 4.4SALFGSDD134 pKa = 3.48PLTKK138 pKa = 10.11RR139 pKa = 11.84SSAPNDD145 pKa = 3.47YY146 pKa = 10.56VSLYY150 pKa = 10.08IEE152 pKa = 3.92QDD154 pKa = 3.12GEE156 pKa = 4.07YY157 pKa = 9.48HH158 pKa = 6.13YY159 pKa = 11.29LGGGYY164 pKa = 9.46RR165 pKa = 11.84EE166 pKa = 4.23GLFLITAAHH175 pKa = 7.06ALTEE179 pKa = 4.61SEE181 pKa = 4.19GHH183 pKa = 5.43TLALSRR189 pKa = 11.84DD190 pKa = 3.3GVKK193 pKa = 10.28FYY195 pKa = 11.17RR196 pKa = 11.84PAGVKK201 pKa = 9.11RR202 pKa = 11.84HH203 pKa = 5.16YY204 pKa = 10.76ARR206 pKa = 11.84EE207 pKa = 4.49GYY209 pKa = 9.86ARR211 pKa = 11.84CTGDD215 pKa = 3.77DD216 pKa = 3.2VGAFEE221 pKa = 6.05LSAADD226 pKa = 3.55WAASGVRR233 pKa = 11.84SVKK236 pKa = 9.91PSVYY240 pKa = 10.09SAVGQTRR247 pKa = 11.84VEE249 pKa = 3.93VFGRR253 pKa = 11.84SRR255 pKa = 11.84QGVLQAGVGALLPPTEE271 pKa = 4.02KK272 pKa = 10.18QKK274 pKa = 11.16RR275 pKa = 11.84LGIVPHH281 pKa = 6.15SASTIRR287 pKa = 11.84GFSGSPVYY295 pKa = 10.08STGEE299 pKa = 3.69SGRR302 pKa = 11.84RR303 pKa = 11.84IVGLHH308 pKa = 5.5IAGGCEE314 pKa = 4.01SDD316 pKa = 3.47NKK318 pKa = 10.95NYY320 pKa = 8.28MASVHH325 pKa = 6.51EE326 pKa = 4.13IHH328 pKa = 7.34FLRR331 pKa = 11.84QKK333 pKa = 10.66LGLVPEE339 pKa = 4.32PQLRR343 pKa = 11.84AEE345 pKa = 4.15VSPNTKK351 pKa = 8.75HH352 pKa = 5.7FQYY355 pKa = 10.5NRR357 pKa = 11.84HH358 pKa = 6.29DD359 pKa = 3.89RR360 pKa = 11.84DD361 pKa = 3.35SDD363 pKa = 3.71EE364 pKa = 5.21EE365 pKa = 4.08EE366 pKa = 5.56LDD368 pKa = 5.36DD369 pKa = 5.8EE370 pKa = 5.43LDD372 pKa = 3.59NANKK376 pKa = 10.34FGGTFGFYY384 pKa = 10.71DD385 pKa = 4.18SFAEE389 pKa = 4.16TAPVSAASAGAVTNPPPGLTPIVEE413 pKa = 4.49GAEE416 pKa = 4.24SEE418 pKa = 4.26PSPEE422 pKa = 3.83EE423 pKa = 3.89SGVGSEE429 pKa = 4.71APGAEE434 pKa = 4.07APSAEE439 pKa = 4.35GDD441 pKa = 3.46RR442 pKa = 11.84DD443 pKa = 4.07DD444 pKa = 6.73DD445 pKa = 3.96VLQVQDD451 pKa = 3.36AFEE454 pKa = 4.22EE455 pKa = 4.34EE456 pKa = 5.05EE457 pKa = 4.72YY458 pKa = 11.34VVLPDD463 pKa = 4.65IEE465 pKa = 5.19APPPPPAGAEE475 pKa = 3.86FVRR478 pKa = 11.84RR479 pKa = 11.84KK480 pKa = 9.97KK481 pKa = 10.51VGNIGRR487 pKa = 11.84FFGCTAFAGAAVAATNKK504 pKa = 9.45VFDD507 pKa = 4.71PAWMVCDD514 pKa = 4.51GPSLRR519 pKa = 11.84AAAGADD525 pKa = 3.51YY526 pKa = 11.23KK527 pKa = 11.86SMFEE531 pKa = 3.97STNFEE536 pKa = 4.25RR537 pKa = 11.84YY538 pKa = 8.99RR539 pKa = 11.84EE540 pKa = 4.04YY541 pKa = 11.22LSAARR546 pKa = 11.84AEE548 pKa = 4.19AAEE551 pKa = 4.67DD552 pKa = 3.37ARR554 pKa = 11.84TMKK557 pKa = 10.7GVDD560 pKa = 3.48GNDD563 pKa = 3.2VARR566 pKa = 11.84LFASGVSNRR575 pKa = 11.84SRR577 pKa = 11.84KK578 pKa = 9.3KK579 pKa = 9.34ALKK582 pKa = 9.82RR583 pKa = 11.84LPEE586 pKa = 4.36RR587 pKa = 11.84FLEE590 pKa = 4.86AIGRR594 pKa = 11.84LGLDD598 pKa = 3.47TKK600 pKa = 11.19AYY602 pKa = 8.51TGWANPPTGVEE613 pKa = 3.92AMEE616 pKa = 4.18QSFKK620 pKa = 10.97VQLGGVKK627 pKa = 8.09PTCWPEE633 pKa = 3.52ATRR636 pKa = 11.84KK637 pKa = 9.75QFVAKK642 pKa = 10.21EE643 pKa = 4.14GPLYY647 pKa = 10.69EE648 pKa = 5.5DD649 pKa = 4.85FLTEE653 pKa = 3.99VAKK656 pKa = 11.18YY657 pKa = 8.35PANRR661 pKa = 11.84YY662 pKa = 8.39NAFTGVHH669 pKa = 5.57ARR671 pKa = 11.84LTRR674 pKa = 11.84FVLGLDD680 pKa = 3.78GDD682 pKa = 4.54KK683 pKa = 11.1SAGWSQHH690 pKa = 4.84FRR692 pKa = 11.84PGTKK696 pKa = 9.79RR697 pKa = 11.84SWQDD701 pKa = 3.11KK702 pKa = 10.31EE703 pKa = 4.18GLEE706 pKa = 4.08LASYY710 pKa = 7.63LTRR713 pKa = 11.84CRR715 pKa = 11.84LLLRR719 pKa = 11.84AAVGPDD725 pKa = 2.96TMAQMTPAQLVGAGLSDD742 pKa = 3.73PRR744 pKa = 11.84TMFVKK749 pKa = 10.64VEE751 pKa = 3.83PHH753 pKa = 6.26NDD755 pKa = 2.88AKK757 pKa = 10.88VAAGRR762 pKa = 11.84WRR764 pKa = 11.84LIWGASLVDD773 pKa = 3.43VCTASVTCRR782 pKa = 11.84KK783 pKa = 9.01QDD785 pKa = 3.32KK786 pKa = 10.86LDD788 pKa = 3.38IEE790 pKa = 4.74QYY792 pKa = 10.13QGGPIPGGHH801 pKa = 5.0QQASGLGHH809 pKa = 7.21HH810 pKa = 7.29DD811 pKa = 3.66LGIDD815 pKa = 3.34RR816 pKa = 11.84LSRR819 pKa = 11.84EE820 pKa = 3.95FDD822 pKa = 3.27RR823 pKa = 11.84LLEE826 pKa = 4.38TGLEE830 pKa = 4.2VFDD833 pKa = 6.08ADD835 pKa = 3.7AQHH838 pKa = 6.71WDD840 pKa = 3.31MLVNRR845 pKa = 11.84DD846 pKa = 4.28SLYY849 pKa = 9.92ADD851 pKa = 3.5AWRR854 pKa = 11.84RR855 pKa = 11.84IILYY859 pKa = 10.27DD860 pKa = 3.79GSNKK864 pKa = 10.28DD865 pKa = 3.54VFEE868 pKa = 4.07MMALCEE874 pKa = 3.83AAANSAHH881 pKa = 5.42VVLVGGNLWEE891 pKa = 4.05ILKK894 pKa = 10.36PGITASGILSTTAQNSFIRR913 pKa = 11.84ALLYY917 pKa = 10.33SFVGIKK923 pKa = 9.98HH924 pKa = 5.89CVVAGDD930 pKa = 4.04DD931 pKa = 3.87AVGARR936 pKa = 11.84QKK938 pKa = 11.18GYY940 pKa = 8.46DD941 pKa = 3.58HH942 pKa = 7.34RR943 pKa = 11.84SALAEE948 pKa = 3.79YY949 pKa = 10.54GPIEE953 pKa = 4.19KK954 pKa = 10.12AVNVYY959 pKa = 9.13TPEE962 pKa = 5.19AGIEE966 pKa = 4.2FTSHH970 pKa = 6.95RR971 pKa = 11.84FTKK974 pKa = 10.55SAGVWTARR982 pKa = 11.84FLNLGKK988 pKa = 10.52ACARR992 pKa = 11.84LALGEE997 pKa = 4.2KK998 pKa = 9.36EE999 pKa = 4.21VRR1001 pKa = 11.84QDD1003 pKa = 3.45QLSGLLFCVRR1013 pKa = 11.84HH1014 pKa = 6.78DD1015 pKa = 3.51EE1016 pKa = 4.24AQIRR1020 pKa = 11.84NLGQVAEE1027 pKa = 4.1QMGWPIAGAEE1037 pKa = 4.13PVFLPCLDD1045 pKa = 3.59

Molecular weight: 113.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEE8|A0A1L3KEE8_9VIRU Putative capsid protein OS=Beihai sobemo-like virus 8 OX=1922705 PE=4 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84NGRR6 pKa = 11.84RR7 pKa = 11.84PDD9 pKa = 3.24RR10 pKa = 11.84PPRR13 pKa = 11.84RR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84PPFRR21 pKa = 11.84RR22 pKa = 11.84GGRR25 pKa = 11.84RR26 pKa = 11.84STAEE30 pKa = 3.52RR31 pKa = 11.84VQAQGVGCVAPVPFGGKK48 pKa = 8.74RR49 pKa = 11.84VASIQGWNAFSPAHH63 pKa = 6.19LPLPRR68 pKa = 11.84SVGPYY73 pKa = 9.69CIVRR77 pKa = 11.84TTTRR81 pKa = 11.84VFSNKK86 pKa = 9.36KK87 pKa = 9.67FVTITCTLSEE97 pKa = 4.86DD98 pKa = 4.11GTWQAVCGFAEE109 pKa = 4.74GDD111 pKa = 3.77LGFGSAIDD119 pKa = 4.05GANNTQVLGSTFPGLTAGAGTSAITVVPSALSVQILNGEE158 pKa = 4.22ALQTTNGILAAGVVKK173 pKa = 9.06TQLALQGRR181 pKa = 11.84TEE183 pKa = 3.73TWEE186 pKa = 3.93AFSNRR191 pKa = 11.84FLAYY195 pKa = 7.33MAPRR199 pKa = 11.84LMSAGKK205 pKa = 9.58LALKK209 pKa = 9.48GVQMNSYY216 pKa = 8.76PLNMNVLSYY225 pKa = 8.71FAPLDD230 pKa = 3.5PTVFSGNITWKK241 pKa = 10.42SGEE244 pKa = 3.97NFQIYY249 pKa = 9.44PEE251 pKa = 4.51GWAPMCILNQDD262 pKa = 3.69AVDD265 pKa = 3.47ITYY268 pKa = 10.72LIAQEE273 pKa = 3.63WRR275 pKa = 11.84VRR277 pKa = 11.84FDD279 pKa = 3.72LANPAASAHH288 pKa = 5.47RR289 pKa = 11.84HH290 pKa = 5.68HH291 pKa = 7.04GVTSDD296 pKa = 3.74GLWDD300 pKa = 4.02KK301 pKa = 9.77MVSMASALGHH311 pKa = 6.08GVQDD315 pKa = 3.74LPEE318 pKa = 5.1FIANVGSAARR328 pKa = 11.84AANQTLRR335 pKa = 11.84MINAGTHH342 pKa = 5.25YY343 pKa = 10.98LPMLTGG349 pKa = 3.68

Molecular weight: 37.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1394

349

1045

697.0

75.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.765 ± 0.144

1.435 ± 0.001

5.093 ± 0.918

5.739 ± 1.359

4.448 ± 0.071

9.828 ± 0.095

2.08 ± 0.171

3.3 ± 0.337

3.802 ± 0.714

8.465 ± 0.209

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.937 ± 0.439

3.156 ± 1.082

5.308 ± 0.471

3.443 ± 0.404

6.6 ± 0.267

6.169 ± 0.064

5.165 ± 0.945

7.604 ± 0.208

1.793 ± 0.1

2.869 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski