Flagellimonas maritima
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z4LUC1|A0A2Z4LUC1_9FLAO Acyl-CoA 6-desaturase OS=Flagellimonas maritima OX=1383885 GN=HME9304_02346 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.23 KK3 pKa = 10.32 FYY5 pKa = 11.08 ALFPILGLFFIACSDD20 pKa = 3.85 DD21 pKa = 5.28 DD22 pKa = 4.14 EE23 pKa = 5.17 TPSEE27 pKa = 4.39 TTPTPDD33 pKa = 4.62 PIVYY37 pKa = 9.36 TSGSADD43 pKa = 3.32 FSNYY47 pKa = 7.91 VAVGNSLTAGFSDD60 pKa = 3.33 NALFRR65 pKa = 11.84 AGQEE69 pKa = 3.63 ASFPNMLAANFALAGGGTFEE89 pKa = 4.9 IPFMADD95 pKa = 2.77 NLGGATINGQTDD107 pKa = 3.5 ANMDD111 pKa = 3.77 GAPDD115 pKa = 3.19 IGNRR119 pKa = 11.84 LILLFTPDD127 pKa = 4.09 GPAPTPKK134 pKa = 9.68 TGVGTTEE141 pKa = 3.81 ITDD144 pKa = 3.81 KK145 pKa = 11.37 LSGPFNNMGVPGSKK159 pKa = 9.74 SYY161 pKa = 11.06 EE162 pKa = 3.98 LLAPGYY168 pKa = 10.64 GSLAGLAVGAANPYY182 pKa = 9.37 FVRR185 pKa = 11.84 FSSGEE190 pKa = 3.76 AATVIGDD197 pKa = 4.28 AAAQNPTFFSLWTGSNDD214 pKa = 2.65 ILLYY218 pKa = 9.5 ATSGGTGTFQSDD230 pKa = 3.93 PMVSPADD237 pKa = 3.62 YY238 pKa = 11.01 ARR240 pKa = 11.84 NDD242 pKa = 3.12 ITNPTVFAGALDD254 pKa = 4.0 AALQALTAGDD264 pKa = 3.88 TDD266 pKa = 4.1 GVIANLPNVTDD277 pKa = 3.53 IPFFTTVPHH286 pKa = 6.28 NPVPLDD292 pKa = 3.49 EE293 pKa = 4.6 ATAAQLNAAYY303 pKa = 10.31 ALYY306 pKa = 10.83 NGGLLQAQGFNLISEE321 pKa = 4.49 EE322 pKa = 4.08 EE323 pKa = 4.24 VAKK326 pKa = 9.79 RR327 pKa = 11.84 TISFTAGEE335 pKa = 4.25 GNAVVLLDD343 pKa = 4.76 EE344 pKa = 5.72 DD345 pKa = 4.17 LTDD348 pKa = 3.73 LTGVNPALINMRR360 pKa = 11.84 QATEE364 pKa = 3.78 EE365 pKa = 4.13 DD366 pKa = 4.36 LLVLTARR373 pKa = 11.84 TFIGTEE379 pKa = 3.95 AVVGDD384 pKa = 3.93 PTTINGIAVPLADD397 pKa = 3.3 QWVLTPEE404 pKa = 3.87 EE405 pKa = 3.86 QANIEE410 pKa = 4.34 TALTAYY416 pKa = 9.87 NQTITALATQYY427 pKa = 11.6 DD428 pKa = 4.4 LALVDD433 pKa = 4.23 VNSLLSEE440 pKa = 3.95 LRR442 pKa = 11.84 EE443 pKa = 4.05 NGIPLADD450 pKa = 4.01 GSTVTATFATGGGFSLDD467 pKa = 3.48 GVHH470 pKa = 7.01 PSPRR474 pKa = 11.84 GYY476 pKa = 10.95 AIIANAFVAAINAKK490 pKa = 10.13 YY491 pKa = 10.42 GSNLPGVNPLDD502 pKa = 3.79 YY503 pKa = 10.61 TGLYY507 pKa = 9.52 IDD509 pKa = 4.39
Molecular weight: 52.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.516
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.414
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.567
EMBOSS 3.681
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.783
Protein with the highest isoelectric point:
>tr|A0A2Z4LSW2|A0A2Z4LSW2_9FLAO Uncharacterized protein OS=Flagellimonas maritima OX=1383885 GN=HME9304_01677 PE=4 SV=1
MM1 pKa = 8.08 RR2 pKa = 11.84 GQLQVASNNPKK13 pKa = 9.33 FKK15 pKa = 10.75 FFILHH20 pKa = 6.37 PSPFIHH26 pKa = 6.62 HH27 pKa = 7.13 PSSITLHH34 pKa = 6.19 PSPFIHH40 pKa = 6.61 HH41 pKa = 7.03 PSSVTHH47 pKa = 6.43 HH48 pKa = 6.81 PSPFTRR54 pKa = 11.84 HH55 pKa = 5.94 PSPIIRR61 pKa = 11.84 HH62 pKa = 5.71 PSPVTRR68 pKa = 11.84 HH69 pKa = 5.8 PSPVTRR75 pKa = 11.84 HH76 pKa = 5.82 PSPFTRR82 pKa = 11.84 HH83 pKa = 5.94 LSPFARR89 pKa = 11.84 HH90 pKa = 6.24 LSPIPRR96 pKa = 11.84 HH97 pKa = 5.56 PSPMILHH104 pKa = 6.74 PSPFTRR110 pKa = 11.84 HH111 pKa = 5.92 RR112 pKa = 11.84 SPFTRR117 pKa = 11.84 HH118 pKa = 5.94 PSPFTRR124 pKa = 11.84 HH125 pKa = 5.12 PP126 pKa = 3.52
Molecular weight: 14.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.618
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.34
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.224
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3376
0
3376
1140652
29
4759
337.9
38.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.15 ± 0.037
0.733 ± 0.013
5.793 ± 0.048
6.713 ± 0.042
5.339 ± 0.032
6.729 ± 0.045
1.751 ± 0.021
7.786 ± 0.037
7.531 ± 0.069
9.436 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.022
5.992 ± 0.036
3.509 ± 0.024
3.268 ± 0.02
3.709 ± 0.025
6.549 ± 0.034
5.596 ± 0.05
6.146 ± 0.031
1.096 ± 0.017
3.953 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here