Amino acid dipepetide frequency for Streptococcus phage SW9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.313AlaAla: 2.313 ± 0.874
0.193AlaCys: 0.193 ± 0.121
3.662AlaAsp: 3.662 ± 0.545
3.18AlaGlu: 3.18 ± 0.484
2.409AlaPhe: 2.409 ± 0.601
3.854AlaGly: 3.854 ± 0.622
0.964AlaHis: 0.964 ± 0.377
5.396AlaIle: 5.396 ± 0.867
6.938AlaLys: 6.938 ± 1.142
5.974AlaLeu: 5.974 ± 0.707
1.445AlaMet: 1.445 ± 0.382
4.722AlaAsn: 4.722 ± 0.736
1.542AlaPro: 1.542 ± 0.314
2.698AlaGln: 2.698 ± 0.464
2.698AlaArg: 2.698 ± 0.478
5.011AlaSer: 5.011 ± 0.726
4.722AlaThr: 4.722 ± 0.866
3.951AlaVal: 3.951 ± 0.633
0.964AlaTrp: 0.964 ± 0.294
1.831AlaTyr: 1.831 ± 0.421
0.0AlaXaa: 0.0 ± 0.0
Cys
0.193CysAla: 0.193 ± 0.112
0.0CysCys: 0.0 ± 0.0
0.675CysAsp: 0.675 ± 0.247
0.193CysGlu: 0.193 ± 0.14
0.193CysPhe: 0.193 ± 0.111
0.096CysGly: 0.096 ± 0.097
0.096CysHis: 0.096 ± 0.094
0.289CysIle: 0.289 ± 0.157
0.385CysLys: 0.385 ± 0.186
0.289CysLeu: 0.289 ± 0.199
0.0CysMet: 0.0 ± 0.0
0.193CysAsn: 0.193 ± 0.116
0.096CysPro: 0.096 ± 0.097
0.289CysGln: 0.289 ± 0.157
0.385CysArg: 0.385 ± 0.207
0.193CysSer: 0.193 ± 0.137
0.289CysThr: 0.289 ± 0.138
0.193CysVal: 0.193 ± 0.113
0.096CysTrp: 0.096 ± 0.093
0.289CysTyr: 0.289 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
3.951AspAla: 3.951 ± 0.546
0.289AspCys: 0.289 ± 0.185
4.336AspAsp: 4.336 ± 0.57
4.24AspGlu: 4.24 ± 0.568
3.951AspPhe: 3.951 ± 0.787
5.685AspGly: 5.685 ± 0.773
0.964AspHis: 0.964 ± 0.278
4.336AspIle: 4.336 ± 0.701
5.589AspLys: 5.589 ± 0.703
4.336AspLeu: 4.336 ± 0.758
2.216AspMet: 2.216 ± 0.404
4.432AspAsn: 4.432 ± 0.624
2.216AspPro: 2.216 ± 0.388
1.253AspGln: 1.253 ± 0.258
2.698AspArg: 2.698 ± 0.409
2.987AspSer: 2.987 ± 0.586
3.662AspThr: 3.662 ± 0.653
4.336AspVal: 4.336 ± 0.791
0.867AspTrp: 0.867 ± 0.255
2.794AspTyr: 2.794 ± 0.585
0.0AspXaa: 0.0 ± 0.0
Glu
4.529GluAla: 4.529 ± 0.517
0.385GluCys: 0.385 ± 0.163
3.469GluAsp: 3.469 ± 0.526
4.336GluGlu: 4.336 ± 0.953
2.698GluPhe: 2.698 ± 0.577
3.662GluGly: 3.662 ± 0.485
0.964GluHis: 0.964 ± 0.322
6.552GluIle: 6.552 ± 0.898
3.469GluLys: 3.469 ± 0.847
6.263GluLeu: 6.263 ± 0.938
1.638GluMet: 1.638 ± 0.414
4.336GluAsn: 4.336 ± 0.631
2.505GluPro: 2.505 ± 0.577
3.18GluGln: 3.18 ± 0.464
3.373GluArg: 3.373 ± 0.566
3.565GluSer: 3.565 ± 0.41
3.18GluThr: 3.18 ± 0.487
4.24GluVal: 4.24 ± 0.755
1.156GluTrp: 1.156 ± 0.256
3.18GluTyr: 3.18 ± 0.713
0.0GluXaa: 0.0 ± 0.0
Phe
3.469PheAla: 3.469 ± 0.54
0.193PheCys: 0.193 ± 0.127
3.951PheAsp: 3.951 ± 0.651
2.12PheGlu: 2.12 ± 0.568
2.12PhePhe: 2.12 ± 0.337
3.469PheGly: 3.469 ± 0.654
0.675PheHis: 0.675 ± 0.253
2.698PheIle: 2.698 ± 0.529
4.143PheLys: 4.143 ± 0.735
3.18PheLeu: 3.18 ± 0.654
0.867PheMet: 0.867 ± 0.299
3.469PheAsn: 3.469 ± 0.746
0.578PhePro: 0.578 ± 0.222
1.349PheGln: 1.349 ± 0.325
1.734PheArg: 1.734 ± 0.286
2.698PheSer: 2.698 ± 0.517
2.987PheThr: 2.987 ± 0.572
2.602PheVal: 2.602 ± 0.375
0.675PheTrp: 0.675 ± 0.228
1.542PheTyr: 1.542 ± 0.423
0.0PheXaa: 0.0 ± 0.0
Gly
3.083GlyAla: 3.083 ± 0.647
0.289GlyCys: 0.289 ± 0.154
4.722GlyAsp: 4.722 ± 0.595
4.24GlyGlu: 4.24 ± 0.677
3.565GlyPhe: 3.565 ± 0.617
5.011GlyGly: 5.011 ± 0.841
0.771GlyHis: 0.771 ± 0.21
5.3GlyIle: 5.3 ± 0.764
6.263GlyLys: 6.263 ± 0.656
5.396GlyLeu: 5.396 ± 0.836
1.349GlyMet: 1.349 ± 0.393
3.469GlyAsn: 3.469 ± 0.547
0.964GlyPro: 0.964 ± 0.262
2.794GlyGln: 2.794 ± 0.66
2.698GlyArg: 2.698 ± 0.435
4.625GlySer: 4.625 ± 0.831
4.432GlyThr: 4.432 ± 0.752
3.469GlyVal: 3.469 ± 0.633
1.253GlyTrp: 1.253 ± 0.305
2.698GlyTyr: 2.698 ± 0.37
0.0GlyXaa: 0.0 ± 0.0
His
0.289HisAla: 0.289 ± 0.148
0.0HisCys: 0.0 ± 0.0
0.771HisAsp: 0.771 ± 0.231
0.385HisGlu: 0.385 ± 0.184
0.675HisPhe: 0.675 ± 0.204
0.964HisGly: 0.964 ± 0.316
0.578HisHis: 0.578 ± 0.203
0.771HisIle: 0.771 ± 0.252
0.964HisLys: 0.964 ± 0.278
1.638HisLeu: 1.638 ± 0.3
0.289HisMet: 0.289 ± 0.168
0.771HisAsn: 0.771 ± 0.305
0.867HisPro: 0.867 ± 0.249
0.578HisGln: 0.578 ± 0.287
0.675HisArg: 0.675 ± 0.222
0.867HisSer: 0.867 ± 0.203
0.964HisThr: 0.964 ± 0.218
1.542HisVal: 1.542 ± 0.311
0.096HisTrp: 0.096 ± 0.086
0.964HisTyr: 0.964 ± 0.357
0.0HisXaa: 0.0 ± 0.0
Ile
5.396IleAla: 5.396 ± 1.039
0.578IleCys: 0.578 ± 0.202
5.878IleAsp: 5.878 ± 0.724
4.722IleGlu: 4.722 ± 0.845
1.927IlePhe: 1.927 ± 0.424
4.818IleGly: 4.818 ± 0.533
0.675IleHis: 0.675 ± 0.24
4.143IleIle: 4.143 ± 0.883
6.841IleLys: 6.841 ± 0.699
3.854IleLeu: 3.854 ± 0.614
1.927IleMet: 1.927 ± 0.552
3.854IleAsn: 3.854 ± 0.58
3.18IlePro: 3.18 ± 0.632
2.794IleGln: 2.794 ± 0.531
2.891IleArg: 2.891 ± 0.57
4.24IleSer: 4.24 ± 0.498
3.758IleThr: 3.758 ± 0.532
2.698IleVal: 2.698 ± 0.545
0.867IleTrp: 0.867 ± 0.165
2.505IleTyr: 2.505 ± 0.479
0.0IleXaa: 0.0 ± 0.0
Lys
4.914LysAla: 4.914 ± 0.613
0.193LysCys: 0.193 ± 0.141
4.529LysAsp: 4.529 ± 0.857
6.552LysGlu: 6.552 ± 0.957
3.276LysPhe: 3.276 ± 0.89
4.914LysGly: 4.914 ± 0.624
1.349LysHis: 1.349 ± 0.409
4.914LysIle: 4.914 ± 0.624
7.42LysLys: 7.42 ± 1.184
6.649LysLeu: 6.649 ± 0.874
2.216LysMet: 2.216 ± 0.431
5.781LysAsn: 5.781 ± 0.594
3.662LysPro: 3.662 ± 0.592
3.854LysGln: 3.854 ± 0.591
3.083LysArg: 3.083 ± 0.418
4.336LysSer: 4.336 ± 0.517
5.396LysThr: 5.396 ± 0.786
4.336LysVal: 4.336 ± 0.568
1.06LysTrp: 1.06 ± 0.263
3.565LysTyr: 3.565 ± 0.742
0.0LysXaa: 0.0 ± 0.0
Leu
7.516LeuAla: 7.516 ± 0.797
0.289LeuCys: 0.289 ± 0.148
5.685LeuAsp: 5.685 ± 0.639
5.781LeuGlu: 5.781 ± 0.818
2.891LeuPhe: 2.891 ± 0.327
5.107LeuGly: 5.107 ± 1.038
0.867LeuHis: 0.867 ± 0.361
4.529LeuIle: 4.529 ± 0.66
5.974LeuLys: 5.974 ± 0.801
4.914LeuLeu: 4.914 ± 0.773
2.024LeuMet: 2.024 ± 0.345
5.685LeuAsn: 5.685 ± 0.721
2.987LeuPro: 2.987 ± 0.51
2.505LeuGln: 2.505 ± 0.52
3.854LeuArg: 3.854 ± 0.692
5.203LeuSer: 5.203 ± 0.779
5.3LeuThr: 5.3 ± 1.012
4.143LeuVal: 4.143 ± 0.553
1.06LeuTrp: 1.06 ± 0.318
2.12LeuTyr: 2.12 ± 0.391
0.0LeuXaa: 0.0 ± 0.0
Met
1.638MetAla: 1.638 ± 0.332
0.0MetCys: 0.0 ± 0.0
1.06MetAsp: 1.06 ± 0.313
1.734MetGlu: 1.734 ± 0.568
1.734MetPhe: 1.734 ± 0.297
1.06MetGly: 1.06 ± 0.363
0.385MetHis: 0.385 ± 0.186
1.445MetIle: 1.445 ± 0.357
2.794MetLys: 2.794 ± 0.42
1.831MetLeu: 1.831 ± 0.303
0.289MetMet: 0.289 ± 0.159
0.964MetAsn: 0.964 ± 0.245
0.867MetPro: 0.867 ± 0.185
0.867MetGln: 0.867 ± 0.298
0.867MetArg: 0.867 ± 0.221
1.445MetSer: 1.445 ± 0.305
1.542MetThr: 1.542 ± 0.35
1.638MetVal: 1.638 ± 0.404
0.289MetTrp: 0.289 ± 0.121
0.578MetTyr: 0.578 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
4.529AsnAla: 4.529 ± 1.141
0.289AsnCys: 0.289 ± 0.161
3.469AsnAsp: 3.469 ± 0.425
4.143AsnGlu: 4.143 ± 0.871
2.891AsnPhe: 2.891 ± 0.562
7.034AsnGly: 7.034 ± 1.254
1.06AsnHis: 1.06 ± 0.312
3.469AsnIle: 3.469 ± 0.665
3.373AsnLys: 3.373 ± 0.515
5.589AsnLeu: 5.589 ± 0.726
1.156AsnMet: 1.156 ± 0.286
4.529AsnAsn: 4.529 ± 0.86
2.794AsnPro: 2.794 ± 0.46
2.698AsnGln: 2.698 ± 0.382
2.313AsnArg: 2.313 ± 0.477
4.047AsnSer: 4.047 ± 0.585
3.951AsnThr: 3.951 ± 0.504
3.469AsnVal: 3.469 ± 0.55
1.06AsnTrp: 1.06 ± 0.263
2.12AsnTyr: 2.12 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
1.831ProAla: 1.831 ± 0.385
0.193ProCys: 0.193 ± 0.134
1.927ProAsp: 1.927 ± 0.427
2.891ProGlu: 2.891 ± 0.507
1.156ProPhe: 1.156 ± 0.285
1.253ProGly: 1.253 ± 0.387
0.482ProHis: 0.482 ± 0.194
1.734ProIle: 1.734 ± 0.339
3.662ProLys: 3.662 ± 0.663
2.505ProLeu: 2.505 ± 0.404
0.289ProMet: 0.289 ± 0.161
2.698ProAsn: 2.698 ± 0.478
0.867ProPro: 0.867 ± 0.315
1.06ProGln: 1.06 ± 0.284
1.06ProArg: 1.06 ± 0.357
3.18ProSer: 3.18 ± 0.632
2.313ProThr: 2.313 ± 0.369
1.927ProVal: 1.927 ± 0.464
0.289ProTrp: 0.289 ± 0.133
1.349ProTyr: 1.349 ± 0.453
0.0ProXaa: 0.0 ± 0.0
Gln
3.662GlnAla: 3.662 ± 0.508
0.193GlnCys: 0.193 ± 0.128
1.638GlnAsp: 1.638 ± 0.472
3.373GlnGlu: 3.373 ± 0.539
1.445GlnPhe: 1.445 ± 0.343
2.891GlnGly: 2.891 ± 0.579
0.385GlnHis: 0.385 ± 0.157
2.794GlnIle: 2.794 ± 0.572
2.698GlnLys: 2.698 ± 0.568
3.373GlnLeu: 3.373 ± 0.555
1.06GlnMet: 1.06 ± 0.327
2.794GlnAsn: 2.794 ± 0.511
0.675GlnPro: 0.675 ± 0.252
2.505GlnGln: 2.505 ± 0.478
1.927GlnArg: 1.927 ± 0.345
2.891GlnSer: 2.891 ± 0.475
2.794GlnThr: 2.794 ± 0.567
2.12GlnVal: 2.12 ± 0.505
0.578GlnTrp: 0.578 ± 0.237
1.445GlnTyr: 1.445 ± 0.364
0.0GlnXaa: 0.0 ± 0.0
Arg
2.024ArgAla: 2.024 ± 0.385
0.096ArgCys: 0.096 ± 0.083
2.505ArgAsp: 2.505 ± 0.535
2.698ArgGlu: 2.698 ± 0.547
2.698ArgPhe: 2.698 ± 0.501
2.409ArgGly: 2.409 ± 0.398
1.06ArgHis: 1.06 ± 0.327
3.083ArgIle: 3.083 ± 0.595
2.891ArgLys: 2.891 ± 0.526
3.565ArgLeu: 3.565 ± 0.514
1.349ArgMet: 1.349 ± 0.381
2.024ArgAsn: 2.024 ± 0.382
1.06ArgPro: 1.06 ± 0.257
2.12ArgGln: 2.12 ± 0.39
1.253ArgArg: 1.253 ± 0.355
2.216ArgSer: 2.216 ± 0.354
2.602ArgThr: 2.602 ± 0.624
2.698ArgVal: 2.698 ± 0.524
1.253ArgTrp: 1.253 ± 0.254
2.024ArgTyr: 2.024 ± 0.508
0.0ArgXaa: 0.0 ± 0.0
Ser
4.143SerAla: 4.143 ± 0.67
0.193SerCys: 0.193 ± 0.121
4.143SerAsp: 4.143 ± 0.539
4.047SerGlu: 4.047 ± 0.44
2.794SerPhe: 2.794 ± 0.42
4.529SerGly: 4.529 ± 0.676
0.385SerHis: 0.385 ± 0.173
4.24SerIle: 4.24 ± 0.527
5.203SerLys: 5.203 ± 0.884
4.722SerLeu: 4.722 ± 0.591
1.734SerMet: 1.734 ± 0.375
4.914SerAsn: 4.914 ± 0.639
2.313SerPro: 2.313 ± 0.417
3.276SerGln: 3.276 ± 0.625
2.698SerArg: 2.698 ± 0.63
4.625SerSer: 4.625 ± 1.123
4.432SerThr: 4.432 ± 0.757
4.914SerVal: 4.914 ± 0.826
0.867SerTrp: 0.867 ± 0.369
1.831SerTyr: 1.831 ± 0.372
0.0SerXaa: 0.0 ± 0.0
Thr
4.625ThrAla: 4.625 ± 0.725
0.289ThrCys: 0.289 ± 0.149
3.951ThrAsp: 3.951 ± 0.719
3.662ThrGlu: 3.662 ± 0.392
3.469ThrPhe: 3.469 ± 0.499
3.565ThrGly: 3.565 ± 0.538
1.253ThrHis: 1.253 ± 0.317
4.24ThrIle: 4.24 ± 0.612
5.492ThrLys: 5.492 ± 0.774
5.781ThrLeu: 5.781 ± 0.918
1.445ThrMet: 1.445 ± 0.345
3.276ThrAsn: 3.276 ± 0.493
1.734ThrPro: 1.734 ± 0.383
2.216ThrGln: 2.216 ± 0.471
1.349ThrArg: 1.349 ± 0.257
4.625ThrSer: 4.625 ± 0.772
3.373ThrThr: 3.373 ± 0.593
4.336ThrVal: 4.336 ± 0.534
1.06ThrTrp: 1.06 ± 0.277
3.276ThrTyr: 3.276 ± 0.708
0.0ThrXaa: 0.0 ± 0.0
Val
3.854ValAla: 3.854 ± 0.458
0.385ValCys: 0.385 ± 0.23
5.396ValAsp: 5.396 ± 0.553
4.336ValGlu: 4.336 ± 0.578
1.927ValPhe: 1.927 ± 0.398
3.758ValGly: 3.758 ± 0.432
0.385ValHis: 0.385 ± 0.183
4.24ValIle: 4.24 ± 0.56
4.529ValLys: 4.529 ± 0.566
4.047ValLeu: 4.047 ± 0.699
0.867ValMet: 0.867 ± 0.274
3.565ValAsn: 3.565 ± 0.659
1.927ValPro: 1.927 ± 0.514
1.831ValGln: 1.831 ± 0.395
3.083ValArg: 3.083 ± 0.595
5.011ValSer: 5.011 ± 0.629
4.625ValThr: 4.625 ± 0.884
3.758ValVal: 3.758 ± 0.692
0.964ValTrp: 0.964 ± 0.259
2.313ValTyr: 2.313 ± 0.467
0.0ValXaa: 0.0 ± 0.0
Trp
0.482TrpAla: 0.482 ± 0.202
0.096TrpCys: 0.096 ± 0.094
1.445TrpAsp: 1.445 ± 0.433
0.867TrpGlu: 0.867 ± 0.258
0.771TrpPhe: 0.771 ± 0.26
0.482TrpGly: 0.482 ± 0.207
0.578TrpHis: 0.578 ± 0.195
1.156TrpIle: 1.156 ± 0.376
0.771TrpLys: 0.771 ± 0.289
1.253TrpLeu: 1.253 ± 0.342
0.0TrpMet: 0.0 ± 0.0
0.771TrpAsn: 0.771 ± 0.275
0.193TrpPro: 0.193 ± 0.14
0.867TrpGln: 0.867 ± 0.283
0.675TrpArg: 0.675 ± 0.179
1.831TrpSer: 1.831 ± 0.638
0.578TrpThr: 0.578 ± 0.195
1.542TrpVal: 1.542 ± 0.299
0.289TrpTrp: 0.289 ± 0.109
0.289TrpTyr: 0.289 ± 0.189
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.024TyrAla: 2.024 ± 0.254
0.289TyrCys: 0.289 ± 0.19
2.024TyrAsp: 2.024 ± 0.373
3.18TyrGlu: 3.18 ± 0.582
1.927TyrPhe: 1.927 ± 0.469
1.831TyrGly: 1.831 ± 0.431
0.771TyrHis: 0.771 ± 0.218
2.216TyrIle: 2.216 ± 0.516
2.602TyrLys: 2.602 ± 0.474
3.276TyrLeu: 3.276 ± 0.494
0.771TyrMet: 0.771 ± 0.26
1.734TyrAsn: 1.734 ± 0.39
1.638TyrPro: 1.638 ± 0.494
2.505TyrGln: 2.505 ± 0.359
2.313TyrArg: 2.313 ± 0.651
2.409TyrSer: 2.409 ± 0.638
2.12TyrThr: 2.12 ± 0.505
2.891TyrVal: 2.891 ± 0.477
0.193TyrTrp: 0.193 ± 0.118
1.734TyrTyr: 1.734 ± 0.35
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (10379 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski