Erigeron breviscapus amalgavirus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z6JIK4|A0A2Z6JIK4_9VIRU ORF1p OS=Erigeron breviscapus amalgavirus 2 OX=2069323 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.53TGGTGPSNQVHH12 pKa = 6.84LLGLTPDD19 pKa = 3.65QEE21 pKa = 4.47QEE23 pKa = 3.9QLTALSAGLVAEE35 pKa = 5.13GFPAAIFSRR44 pKa = 11.84TAAIDD49 pKa = 3.34CGYY52 pKa = 10.97DD53 pKa = 3.22FARR56 pKa = 11.84FYY58 pKa = 11.15RR59 pKa = 11.84VVKK62 pKa = 11.11SMDD65 pKa = 4.01DD66 pKa = 3.75LLKK69 pKa = 9.81MDD71 pKa = 5.46LFDD74 pKa = 4.7EE75 pKa = 4.46VLTLAVTGLFFVSPLRR91 pKa = 11.84CTTKK95 pKa = 10.45KK96 pKa = 9.82FCEE99 pKa = 4.02FGAYY103 pKa = 10.23LKK105 pKa = 7.22TTKK108 pKa = 10.43GQEE111 pKa = 4.17ALHH114 pKa = 6.43GAQKK118 pKa = 9.25MKK120 pKa = 10.5KK121 pKa = 8.41YY122 pKa = 7.69QAKK125 pKa = 10.35VIGEE129 pKa = 4.25FEE131 pKa = 4.27PKK133 pKa = 10.26DD134 pKa = 3.6VVLEE138 pKa = 4.17QIFNAQRR145 pKa = 11.84ADD147 pKa = 3.48YY148 pKa = 11.29AEE150 pKa = 4.0VLKK153 pKa = 10.63EE154 pKa = 3.68EE155 pKa = 4.11RR156 pKa = 11.84SNYY159 pKa = 9.48DD160 pKa = 3.28RR161 pKa = 11.84EE162 pKa = 4.06IEE164 pKa = 4.01ALKK167 pKa = 10.51KK168 pKa = 10.33QIRR171 pKa = 11.84LLEE174 pKa = 4.03ARR176 pKa = 11.84KK177 pKa = 9.78EE178 pKa = 3.96EE179 pKa = 4.15RR180 pKa = 11.84LEE182 pKa = 3.86QIAAGFAPASYY193 pKa = 10.55YY194 pKa = 10.6SEE196 pKa = 5.1PDD198 pKa = 3.46PADD201 pKa = 3.31VAVEE205 pKa = 3.8AWEE208 pKa = 4.53MYY210 pKa = 10.72QNDD213 pKa = 3.23ARR215 pKa = 11.84AKK217 pKa = 9.76GKK219 pKa = 10.42VAMSRR224 pKa = 11.84YY225 pKa = 10.17DD226 pKa = 3.55GGDD229 pKa = 2.91KK230 pKa = 10.84YY231 pKa = 11.49AVANFGNKK239 pKa = 7.37VKK241 pKa = 10.59RR242 pKa = 11.84LHH244 pKa = 6.02CLEE247 pKa = 4.15YY248 pKa = 11.03CGDD251 pKa = 3.88DD252 pKa = 3.55ASRR255 pKa = 11.84TLLLEE260 pKa = 3.94YY261 pKa = 10.26CKK263 pKa = 10.74KK264 pKa = 10.59KK265 pKa = 10.37ILAFGPIRR273 pKa = 11.84ISDD276 pKa = 3.83KK277 pKa = 8.96PHH279 pKa = 6.8PSRR282 pKa = 11.84VCWLEE287 pKa = 3.83QVEE290 pKa = 4.55TWLLSRR296 pKa = 11.84PVALRR301 pKa = 11.84RR302 pKa = 11.84EE303 pKa = 4.23ICSLVPVGFPHH314 pKa = 7.84LPQQRR319 pKa = 11.84PEE321 pKa = 4.07CFEE324 pKa = 4.31LSSLVSRR331 pKa = 11.84DD332 pKa = 3.11VLEE335 pKa = 4.74RR336 pKa = 11.84KK337 pKa = 8.92NQLGHH342 pKa = 7.16RR343 pKa = 11.84PIQVLARR350 pKa = 11.84GIRR353 pKa = 11.84AQEE356 pKa = 3.89LDD358 pKa = 3.53SSIMRR363 pKa = 11.84NPRR366 pKa = 11.84LKK368 pKa = 10.84VYY370 pKa = 10.95VNFQGEE376 pKa = 4.26PGRR379 pKa = 11.84VIPHH383 pKa = 5.9SRR385 pKa = 11.84SKK387 pKa = 11.42YY388 pKa = 7.83EE389 pKa = 3.44AALRR393 pKa = 11.84RR394 pKa = 11.84IIGGGAMRR402 pKa = 11.84SWGEE406 pKa = 3.58DD407 pKa = 2.59SKK409 pKa = 10.85MYY411 pKa = 10.49RR412 pKa = 11.84GGGTSSDD419 pKa = 3.51ALLLLSQADD428 pKa = 3.64FRR430 pKa = 11.84LPGGLLKK437 pKa = 10.65EE438 pKa = 4.27HH439 pKa = 6.83FSLRR443 pKa = 11.84TARR446 pKa = 11.84EE447 pKa = 4.02ALCLPEE453 pKa = 5.63DD454 pKa = 4.2LVVPDD459 pKa = 5.38GKK461 pKa = 10.56DD462 pKa = 3.14CCRR465 pKa = 11.84MKK467 pKa = 10.94NFNNEE472 pKa = 3.28ATAGPFLRR480 pKa = 11.84LFGVKK485 pKa = 9.82GKK487 pKa = 10.91YY488 pKa = 8.91GLKK491 pKa = 10.33KK492 pKa = 10.52LLEE495 pKa = 4.51DD496 pKa = 3.92EE497 pKa = 3.9MWRR500 pKa = 11.84YY501 pKa = 10.32YY502 pKa = 11.2DD503 pKa = 5.48DD504 pKa = 4.08FAQGRR509 pKa = 11.84IDD511 pKa = 4.37EE512 pKa = 4.72RR513 pKa = 11.84GLPYY517 pKa = 10.06FAARR521 pKa = 11.84LGFRR525 pKa = 11.84TKK527 pKa = 10.9LMSEE531 pKa = 4.0KK532 pKa = 10.48KK533 pKa = 10.14AWEE536 pKa = 4.09KK537 pKa = 8.82MQKK540 pKa = 9.99GDD542 pKa = 3.42PYY544 pKa = 10.87GRR546 pKa = 11.84AVMMLDD552 pKa = 3.68ALEE555 pKa = 4.23QAASSPLYY563 pKa = 10.51NVLTGVTFEE572 pKa = 4.15RR573 pKa = 11.84RR574 pKa = 11.84LEE576 pKa = 4.12KK577 pKa = 10.84EE578 pKa = 4.19CGFKK582 pKa = 10.82NQVIRR587 pKa = 11.84ASSDD591 pKa = 2.64WNRR594 pKa = 11.84VWEE597 pKa = 4.03YY598 pKa = 11.29LRR600 pKa = 11.84DD601 pKa = 3.59SKK603 pKa = 11.55VIIEE607 pKa = 4.37LDD609 pKa = 2.84WAKK612 pKa = 10.5FDD614 pKa = 4.29RR615 pKa = 11.84EE616 pKa = 4.21RR617 pKa = 11.84PSEE620 pKa = 4.05DD621 pKa = 3.2LQFVIDD627 pKa = 3.93VVLSCFAPRR636 pKa = 11.84TPRR639 pKa = 11.84EE640 pKa = 3.95RR641 pKa = 11.84KK642 pKa = 9.52LLEE645 pKa = 4.15AYY647 pKa = 9.99GIVMRR652 pKa = 11.84RR653 pKa = 11.84ALIEE657 pKa = 3.86RR658 pKa = 11.84AVVMDD663 pKa = 4.19RR664 pKa = 11.84GGVFTIEE671 pKa = 4.0GMVPSGSLWTGWLDD685 pKa = 3.31TALNILYY692 pKa = 10.5LNAACVEE699 pKa = 4.12AGYY702 pKa = 10.94GPGFFHH708 pKa = 6.95PMCAGDD714 pKa = 5.19DD715 pKa = 3.84NLTLFDD721 pKa = 5.68LDD723 pKa = 3.88LGDD726 pKa = 4.82RR727 pKa = 11.84RR728 pKa = 11.84LLKK731 pKa = 10.23IRR733 pKa = 11.84EE734 pKa = 4.06VLNNWFRR741 pKa = 11.84AGISEE746 pKa = 4.26EE747 pKa = 4.21DD748 pKa = 4.05FFIHH752 pKa = 6.96RR753 pKa = 11.84PPFHH757 pKa = 6.73VIKK760 pKa = 10.42KK761 pKa = 7.13QACFPPGTDD770 pKa = 3.98LKK772 pKa = 10.74IGTSKK777 pKa = 10.61RR778 pKa = 11.84MHH780 pKa = 5.81EE781 pKa = 4.28AFWVEE786 pKa = 3.8FDD788 pKa = 3.85GEE790 pKa = 4.34LVIDD794 pKa = 4.24EE795 pKa = 4.4AAGRR799 pKa = 11.84SHH801 pKa = 6.25RR802 pKa = 11.84WEE804 pKa = 4.35YY805 pKa = 10.47IFKK808 pKa = 10.43GRR810 pKa = 11.84PKK812 pKa = 10.37FLSNYY817 pKa = 7.05WLPEE821 pKa = 3.93GQPIRR826 pKa = 11.84PTRR829 pKa = 11.84DD830 pKa = 3.26NLEE833 pKa = 3.98KK834 pKa = 10.88LLWPEE839 pKa = 5.46GIHH842 pKa = 7.16KK843 pKa = 10.32DD844 pKa = 3.53LNEE847 pKa = 3.93YY848 pKa = 9.47QAALMAMVVDD858 pKa = 4.57NPWNHH863 pKa = 6.42HH864 pKa = 5.43AVNHH868 pKa = 5.67LLMRR872 pKa = 11.84YY873 pKa = 9.79VIVQQLRR880 pKa = 11.84RR881 pKa = 11.84VNSGLGRR888 pKa = 11.84EE889 pKa = 4.36DD890 pKa = 3.25DD891 pKa = 4.09TLFLAGLRR899 pKa = 11.84DD900 pKa = 3.57MTGGVIPFPMVAPWRR915 pKa = 11.84RR916 pKa = 11.84GAQQGRR922 pKa = 11.84MEE924 pKa = 5.27DD925 pKa = 3.62YY926 pKa = 11.18KK927 pKa = 11.03EE928 pKa = 4.04VQNWCLDD935 pKa = 3.52FQSFVTGVTSLYY947 pKa = 9.86TRR949 pKa = 11.84EE950 pKa = 4.17ATGGMDD956 pKa = 2.91AWQFMEE962 pKa = 5.32IIRR965 pKa = 11.84GDD967 pKa = 3.38HH968 pKa = 6.31HH969 pKa = 8.67VGEE972 pKa = 4.63GQFGNDD978 pKa = 3.95LMRR981 pKa = 11.84WLQWMASHH989 pKa = 7.4PCSKK993 pKa = 10.15YY994 pKa = 9.73LRR996 pKa = 11.84PVRR999 pKa = 11.84GLRR1002 pKa = 11.84PGKK1005 pKa = 10.2SAVTAEE1011 pKa = 4.46RR1012 pKa = 11.84EE1013 pKa = 4.16DD1014 pKa = 3.73LDD1016 pKa = 3.98RR1017 pKa = 11.84AVNGFNTLRR1026 pKa = 11.84RR1027 pKa = 11.84LLSSGRR1033 pKa = 11.84LCSSLDD1039 pKa = 3.33YY1040 pKa = 11.04ALWISHH1046 pKa = 5.78SVIDD1050 pKa = 4.5SLPGG1054 pKa = 3.25

Molecular weight:
120.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z6JIK4|A0A2Z6JIK4_9VIRU ORF1p OS=Erigeron breviscapus amalgavirus 2 OX=2069323 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.53TGGTGPSNQVHH12 pKa = 6.84LLGLTPDD19 pKa = 3.65QEE21 pKa = 4.47QEE23 pKa = 3.9QLTALSAGLVAEE35 pKa = 5.13GFPAAIFSRR44 pKa = 11.84TAAIDD49 pKa = 3.34CGYY52 pKa = 10.97DD53 pKa = 3.22FARR56 pKa = 11.84FYY58 pKa = 11.15RR59 pKa = 11.84VVKK62 pKa = 11.11SMDD65 pKa = 4.01DD66 pKa = 3.75LLKK69 pKa = 9.81MDD71 pKa = 5.46LFDD74 pKa = 4.7EE75 pKa = 4.46VLTLAVTGLFFVSPLRR91 pKa = 11.84CTTKK95 pKa = 10.45KK96 pKa = 9.82FCEE99 pKa = 4.02FGAYY103 pKa = 10.23LKK105 pKa = 7.22TTKK108 pKa = 10.43GQEE111 pKa = 4.17ALHH114 pKa = 6.43GAQKK118 pKa = 9.25MKK120 pKa = 10.5KK121 pKa = 8.41YY122 pKa = 7.69QAKK125 pKa = 10.35VIGEE129 pKa = 4.25FEE131 pKa = 4.27PKK133 pKa = 10.26DD134 pKa = 3.6VVLEE138 pKa = 4.17QIFNAQRR145 pKa = 11.84ADD147 pKa = 3.48YY148 pKa = 11.29AEE150 pKa = 4.0VLKK153 pKa = 10.63EE154 pKa = 3.68EE155 pKa = 4.11RR156 pKa = 11.84SNYY159 pKa = 9.48DD160 pKa = 3.28RR161 pKa = 11.84EE162 pKa = 4.06IEE164 pKa = 4.01ALKK167 pKa = 10.51KK168 pKa = 10.33QIRR171 pKa = 11.84LLEE174 pKa = 4.03ARR176 pKa = 11.84KK177 pKa = 9.78EE178 pKa = 3.96EE179 pKa = 4.15RR180 pKa = 11.84LEE182 pKa = 3.86QIAAGFAPASYY193 pKa = 10.55YY194 pKa = 10.6SEE196 pKa = 5.1PDD198 pKa = 3.46PADD201 pKa = 3.31VAVEE205 pKa = 3.8AWEE208 pKa = 4.53MYY210 pKa = 10.72QNDD213 pKa = 3.23ARR215 pKa = 11.84AKK217 pKa = 9.76GKK219 pKa = 10.42VAMSRR224 pKa = 11.84YY225 pKa = 10.17DD226 pKa = 3.55GGDD229 pKa = 2.91KK230 pKa = 10.84YY231 pKa = 11.49AVANFGNKK239 pKa = 7.37VKK241 pKa = 10.59RR242 pKa = 11.84LHH244 pKa = 6.02CLEE247 pKa = 4.15YY248 pKa = 11.03CGDD251 pKa = 3.88DD252 pKa = 3.55ASRR255 pKa = 11.84TLLLEE260 pKa = 3.94YY261 pKa = 10.26CKK263 pKa = 10.74KK264 pKa = 10.67KK265 pKa = 10.43ILAFRR270 pKa = 11.84ADD272 pKa = 3.21KK273 pKa = 10.99NKK275 pKa = 10.39RR276 pKa = 11.84QTSSFEE282 pKa = 3.88GMLVGAGGDD291 pKa = 3.77LASVPAGRR299 pKa = 11.84PAAGDD304 pKa = 3.67LLPRR308 pKa = 11.84ARR310 pKa = 11.84GISPPPAATAGVFRR324 pKa = 11.84VEE326 pKa = 4.38QPRR329 pKa = 11.84QPRR332 pKa = 11.84RR333 pKa = 11.84VRR335 pKa = 11.84EE336 pKa = 3.99EE337 pKa = 4.04EE338 pKa = 3.94PAGAPAHH345 pKa = 6.39SGTGSRR351 pKa = 11.84NTRR354 pKa = 11.84ARR356 pKa = 11.84TRR358 pKa = 11.84QQHH361 pKa = 4.95NEE363 pKa = 3.7EE364 pKa = 4.67SEE366 pKa = 4.32VEE368 pKa = 4.25SLRR371 pKa = 11.84EE372 pKa = 3.65FPGRR376 pKa = 11.84ARR378 pKa = 11.84QGNPPQPLL386 pKa = 3.25

Molecular weight:
42.7 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1440

386

1054

720.0

81.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.958 ± 1.641

1.806 ± 0.128

5.833 ± 0.332

7.5 ± 0.535

4.653 ± 0.127

7.917 ± 0.058

1.806 ± 0.26

3.472 ± 0.582

5.694 ± 0.399

10.208 ± 0.846

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.385

2.847 ± 0.131

5.0 ± 0.357

3.889 ± 0.527

8.264 ± 0.119

5.0 ± 0.172

3.611 ± 0.404

6.042 ± 0.174

1.667 ± 0.718

3.264 ± 0.053

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski