Erigeron breviscapus amalgavirus 2
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIK4|A0A2Z6JIK4_9VIRU ORF1p OS=Erigeron breviscapus amalgavirus 2 OX=2069323 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.53 TGGTGPSNQVHH12 pKa = 6.84 LLGLTPDD19 pKa = 3.65 QEE21 pKa = 4.47 QEE23 pKa = 3.9 QLTALSAGLVAEE35 pKa = 5.13 GFPAAIFSRR44 pKa = 11.84 TAAIDD49 pKa = 3.34 CGYY52 pKa = 10.97 DD53 pKa = 3.22 FARR56 pKa = 11.84 FYY58 pKa = 11.15 RR59 pKa = 11.84 VVKK62 pKa = 11.11 SMDD65 pKa = 4.01 DD66 pKa = 3.75 LLKK69 pKa = 9.81 MDD71 pKa = 5.46 LFDD74 pKa = 4.7 EE75 pKa = 4.46 VLTLAVTGLFFVSPLRR91 pKa = 11.84 CTTKK95 pKa = 10.45 KK96 pKa = 9.82 FCEE99 pKa = 4.02 FGAYY103 pKa = 10.23 LKK105 pKa = 7.22 TTKK108 pKa = 10.43 GQEE111 pKa = 4.17 ALHH114 pKa = 6.43 GAQKK118 pKa = 9.25 MKK120 pKa = 10.5 KK121 pKa = 8.41 YY122 pKa = 7.69 QAKK125 pKa = 10.35 VIGEE129 pKa = 4.25 FEE131 pKa = 4.27 PKK133 pKa = 10.26 DD134 pKa = 3.6 VVLEE138 pKa = 4.17 QIFNAQRR145 pKa = 11.84 ADD147 pKa = 3.48 YY148 pKa = 11.29 AEE150 pKa = 4.0 VLKK153 pKa = 10.63 EE154 pKa = 3.68 EE155 pKa = 4.11 RR156 pKa = 11.84 SNYY159 pKa = 9.48 DD160 pKa = 3.28 RR161 pKa = 11.84 EE162 pKa = 4.06 IEE164 pKa = 4.01 ALKK167 pKa = 10.51 KK168 pKa = 10.33 QIRR171 pKa = 11.84 LLEE174 pKa = 4.03 ARR176 pKa = 11.84 KK177 pKa = 9.78 EE178 pKa = 3.96 EE179 pKa = 4.15 RR180 pKa = 11.84 LEE182 pKa = 3.86 QIAAGFAPASYY193 pKa = 10.55 YY194 pKa = 10.6 SEE196 pKa = 5.1 PDD198 pKa = 3.46 PADD201 pKa = 3.31 VAVEE205 pKa = 3.8 AWEE208 pKa = 4.53 MYY210 pKa = 10.72 QNDD213 pKa = 3.23 ARR215 pKa = 11.84 AKK217 pKa = 9.76 GKK219 pKa = 10.42 VAMSRR224 pKa = 11.84 YY225 pKa = 10.17 DD226 pKa = 3.55 GGDD229 pKa = 2.91 KK230 pKa = 10.84 YY231 pKa = 11.49 AVANFGNKK239 pKa = 7.37 VKK241 pKa = 10.59 RR242 pKa = 11.84 LHH244 pKa = 6.02 CLEE247 pKa = 4.15 YY248 pKa = 11.03 CGDD251 pKa = 3.88 DD252 pKa = 3.55 ASRR255 pKa = 11.84 TLLLEE260 pKa = 3.94 YY261 pKa = 10.26 CKK263 pKa = 10.74 KK264 pKa = 10.59 KK265 pKa = 10.37 ILAFGPIRR273 pKa = 11.84 ISDD276 pKa = 3.83 KK277 pKa = 8.96 PHH279 pKa = 6.8 PSRR282 pKa = 11.84 VCWLEE287 pKa = 3.83 QVEE290 pKa = 4.55 TWLLSRR296 pKa = 11.84 PVALRR301 pKa = 11.84 RR302 pKa = 11.84 EE303 pKa = 4.23 ICSLVPVGFPHH314 pKa = 7.84 LPQQRR319 pKa = 11.84 PEE321 pKa = 4.07 CFEE324 pKa = 4.31 LSSLVSRR331 pKa = 11.84 DD332 pKa = 3.11 VLEE335 pKa = 4.74 RR336 pKa = 11.84 KK337 pKa = 8.92 NQLGHH342 pKa = 7.16 RR343 pKa = 11.84 PIQVLARR350 pKa = 11.84 GIRR353 pKa = 11.84 AQEE356 pKa = 3.89 LDD358 pKa = 3.53 SSIMRR363 pKa = 11.84 NPRR366 pKa = 11.84 LKK368 pKa = 10.84 VYY370 pKa = 10.95 VNFQGEE376 pKa = 4.26 PGRR379 pKa = 11.84 VIPHH383 pKa = 5.9 SRR385 pKa = 11.84 SKK387 pKa = 11.42 YY388 pKa = 7.83 EE389 pKa = 3.44 AALRR393 pKa = 11.84 RR394 pKa = 11.84 IIGGGAMRR402 pKa = 11.84 SWGEE406 pKa = 3.58 DD407 pKa = 2.59 SKK409 pKa = 10.85 MYY411 pKa = 10.49 RR412 pKa = 11.84 GGGTSSDD419 pKa = 3.51 ALLLLSQADD428 pKa = 3.64 FRR430 pKa = 11.84 LPGGLLKK437 pKa = 10.65 EE438 pKa = 4.27 HH439 pKa = 6.83 FSLRR443 pKa = 11.84 TARR446 pKa = 11.84 EE447 pKa = 4.02 ALCLPEE453 pKa = 5.63 DD454 pKa = 4.2 LVVPDD459 pKa = 5.38 GKK461 pKa = 10.56 DD462 pKa = 3.14 CCRR465 pKa = 11.84 MKK467 pKa = 10.94 NFNNEE472 pKa = 3.28 ATAGPFLRR480 pKa = 11.84 LFGVKK485 pKa = 9.82 GKK487 pKa = 10.91 YY488 pKa = 8.91 GLKK491 pKa = 10.33 KK492 pKa = 10.52 LLEE495 pKa = 4.51 DD496 pKa = 3.92 EE497 pKa = 3.9 MWRR500 pKa = 11.84 YY501 pKa = 10.32 YY502 pKa = 11.2 DD503 pKa = 5.48 DD504 pKa = 4.08 FAQGRR509 pKa = 11.84 IDD511 pKa = 4.37 EE512 pKa = 4.72 RR513 pKa = 11.84 GLPYY517 pKa = 10.06 FAARR521 pKa = 11.84 LGFRR525 pKa = 11.84 TKK527 pKa = 10.9 LMSEE531 pKa = 4.0 KK532 pKa = 10.48 KK533 pKa = 10.14 AWEE536 pKa = 4.09 KK537 pKa = 8.82 MQKK540 pKa = 9.99 GDD542 pKa = 3.42 PYY544 pKa = 10.87 GRR546 pKa = 11.84 AVMMLDD552 pKa = 3.68 ALEE555 pKa = 4.23 QAASSPLYY563 pKa = 10.51 NVLTGVTFEE572 pKa = 4.15 RR573 pKa = 11.84 RR574 pKa = 11.84 LEE576 pKa = 4.12 KK577 pKa = 10.84 EE578 pKa = 4.19 CGFKK582 pKa = 10.82 NQVIRR587 pKa = 11.84 ASSDD591 pKa = 2.64 WNRR594 pKa = 11.84 VWEE597 pKa = 4.03 YY598 pKa = 11.29 LRR600 pKa = 11.84 DD601 pKa = 3.59 SKK603 pKa = 11.55 VIIEE607 pKa = 4.37 LDD609 pKa = 2.84 WAKK612 pKa = 10.5 FDD614 pKa = 4.29 RR615 pKa = 11.84 EE616 pKa = 4.21 RR617 pKa = 11.84 PSEE620 pKa = 4.05 DD621 pKa = 3.2 LQFVIDD627 pKa = 3.93 VVLSCFAPRR636 pKa = 11.84 TPRR639 pKa = 11.84 EE640 pKa = 3.95 RR641 pKa = 11.84 KK642 pKa = 9.52 LLEE645 pKa = 4.15 AYY647 pKa = 9.99 GIVMRR652 pKa = 11.84 RR653 pKa = 11.84 ALIEE657 pKa = 3.86 RR658 pKa = 11.84 AVVMDD663 pKa = 4.19 RR664 pKa = 11.84 GGVFTIEE671 pKa = 4.0 GMVPSGSLWTGWLDD685 pKa = 3.31 TALNILYY692 pKa = 10.5 LNAACVEE699 pKa = 4.12 AGYY702 pKa = 10.94 GPGFFHH708 pKa = 6.95 PMCAGDD714 pKa = 5.19 DD715 pKa = 3.84 NLTLFDD721 pKa = 5.68 LDD723 pKa = 3.88 LGDD726 pKa = 4.82 RR727 pKa = 11.84 RR728 pKa = 11.84 LLKK731 pKa = 10.23 IRR733 pKa = 11.84 EE734 pKa = 4.06 VLNNWFRR741 pKa = 11.84 AGISEE746 pKa = 4.26 EE747 pKa = 4.21 DD748 pKa = 4.05 FFIHH752 pKa = 6.96 RR753 pKa = 11.84 PPFHH757 pKa = 6.73 VIKK760 pKa = 10.42 KK761 pKa = 7.13 QACFPPGTDD770 pKa = 3.98 LKK772 pKa = 10.74 IGTSKK777 pKa = 10.61 RR778 pKa = 11.84 MHH780 pKa = 5.81 EE781 pKa = 4.28 AFWVEE786 pKa = 3.8 FDD788 pKa = 3.85 GEE790 pKa = 4.34 LVIDD794 pKa = 4.24 EE795 pKa = 4.4 AAGRR799 pKa = 11.84 SHH801 pKa = 6.25 RR802 pKa = 11.84 WEE804 pKa = 4.35 YY805 pKa = 10.47 IFKK808 pKa = 10.43 GRR810 pKa = 11.84 PKK812 pKa = 10.37 FLSNYY817 pKa = 7.05 WLPEE821 pKa = 3.93 GQPIRR826 pKa = 11.84 PTRR829 pKa = 11.84 DD830 pKa = 3.26 NLEE833 pKa = 3.98 KK834 pKa = 10.88 LLWPEE839 pKa = 5.46 GIHH842 pKa = 7.16 KK843 pKa = 10.32 DD844 pKa = 3.53 LNEE847 pKa = 3.93 YY848 pKa = 9.47 QAALMAMVVDD858 pKa = 4.57 NPWNHH863 pKa = 6.42 HH864 pKa = 5.43 AVNHH868 pKa = 5.67 LLMRR872 pKa = 11.84 YY873 pKa = 9.79 VIVQQLRR880 pKa = 11.84 RR881 pKa = 11.84 VNSGLGRR888 pKa = 11.84 EE889 pKa = 4.36 DD890 pKa = 3.25 DD891 pKa = 4.09 TLFLAGLRR899 pKa = 11.84 DD900 pKa = 3.57 MTGGVIPFPMVAPWRR915 pKa = 11.84 RR916 pKa = 11.84 GAQQGRR922 pKa = 11.84 MEE924 pKa = 5.27 DD925 pKa = 3.62 YY926 pKa = 11.18 KK927 pKa = 11.03 EE928 pKa = 4.04 VQNWCLDD935 pKa = 3.52 FQSFVTGVTSLYY947 pKa = 9.86 TRR949 pKa = 11.84 EE950 pKa = 4.17 ATGGMDD956 pKa = 2.91 AWQFMEE962 pKa = 5.32 IIRR965 pKa = 11.84 GDD967 pKa = 3.38 HH968 pKa = 6.31 HH969 pKa = 8.67 VGEE972 pKa = 4.63 GQFGNDD978 pKa = 3.95 LMRR981 pKa = 11.84 WLQWMASHH989 pKa = 7.4 PCSKK993 pKa = 10.15 YY994 pKa = 9.73 LRR996 pKa = 11.84 PVRR999 pKa = 11.84 GLRR1002 pKa = 11.84 PGKK1005 pKa = 10.2 SAVTAEE1011 pKa = 4.46 RR1012 pKa = 11.84 EE1013 pKa = 4.16 DD1014 pKa = 3.73 LDD1016 pKa = 3.98 RR1017 pKa = 11.84 AVNGFNTLRR1026 pKa = 11.84 RR1027 pKa = 11.84 LLSSGRR1033 pKa = 11.84 LCSSLDD1039 pKa = 3.33 YY1040 pKa = 11.04 ALWISHH1046 pKa = 5.78 SVIDD1050 pKa = 4.5 SLPGG1054 pKa = 3.25
Molecular weight: 120.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.946
IPC2_protein 7.366
IPC_protein 7.176
Toseland 6.912
ProMoST 7.79
Dawson 7.892
Bjellqvist 8.302
Wikipedia 7.805
Rodwell 7.907
Grimsley 6.985
Solomon 7.922
Lehninger 7.936
Nozaki 8.521
DTASelect 8.039
Thurlkill 8.097
EMBOSS 8.112
Sillero 8.419
Patrickios 4.482
IPC_peptide 7.922
IPC2_peptide 7.351
IPC2.peptide.svr19 7.338
Protein with the highest isoelectric point:
>tr|A0A2Z6JIK4|A0A2Z6JIK4_9VIRU ORF1p OS=Erigeron breviscapus amalgavirus 2 OX=2069323 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.53 TGGTGPSNQVHH12 pKa = 6.84 LLGLTPDD19 pKa = 3.65 QEE21 pKa = 4.47 QEE23 pKa = 3.9 QLTALSAGLVAEE35 pKa = 5.13 GFPAAIFSRR44 pKa = 11.84 TAAIDD49 pKa = 3.34 CGYY52 pKa = 10.97 DD53 pKa = 3.22 FARR56 pKa = 11.84 FYY58 pKa = 11.15 RR59 pKa = 11.84 VVKK62 pKa = 11.11 SMDD65 pKa = 4.01 DD66 pKa = 3.75 LLKK69 pKa = 9.81 MDD71 pKa = 5.46 LFDD74 pKa = 4.7 EE75 pKa = 4.46 VLTLAVTGLFFVSPLRR91 pKa = 11.84 CTTKK95 pKa = 10.45 KK96 pKa = 9.82 FCEE99 pKa = 4.02 FGAYY103 pKa = 10.23 LKK105 pKa = 7.22 TTKK108 pKa = 10.43 GQEE111 pKa = 4.17 ALHH114 pKa = 6.43 GAQKK118 pKa = 9.25 MKK120 pKa = 10.5 KK121 pKa = 8.41 YY122 pKa = 7.69 QAKK125 pKa = 10.35 VIGEE129 pKa = 4.25 FEE131 pKa = 4.27 PKK133 pKa = 10.26 DD134 pKa = 3.6 VVLEE138 pKa = 4.17 QIFNAQRR145 pKa = 11.84 ADD147 pKa = 3.48 YY148 pKa = 11.29 AEE150 pKa = 4.0 VLKK153 pKa = 10.63 EE154 pKa = 3.68 EE155 pKa = 4.11 RR156 pKa = 11.84 SNYY159 pKa = 9.48 DD160 pKa = 3.28 RR161 pKa = 11.84 EE162 pKa = 4.06 IEE164 pKa = 4.01 ALKK167 pKa = 10.51 KK168 pKa = 10.33 QIRR171 pKa = 11.84 LLEE174 pKa = 4.03 ARR176 pKa = 11.84 KK177 pKa = 9.78 EE178 pKa = 3.96 EE179 pKa = 4.15 RR180 pKa = 11.84 LEE182 pKa = 3.86 QIAAGFAPASYY193 pKa = 10.55 YY194 pKa = 10.6 SEE196 pKa = 5.1 PDD198 pKa = 3.46 PADD201 pKa = 3.31 VAVEE205 pKa = 3.8 AWEE208 pKa = 4.53 MYY210 pKa = 10.72 QNDD213 pKa = 3.23 ARR215 pKa = 11.84 AKK217 pKa = 9.76 GKK219 pKa = 10.42 VAMSRR224 pKa = 11.84 YY225 pKa = 10.17 DD226 pKa = 3.55 GGDD229 pKa = 2.91 KK230 pKa = 10.84 YY231 pKa = 11.49 AVANFGNKK239 pKa = 7.37 VKK241 pKa = 10.59 RR242 pKa = 11.84 LHH244 pKa = 6.02 CLEE247 pKa = 4.15 YY248 pKa = 11.03 CGDD251 pKa = 3.88 DD252 pKa = 3.55 ASRR255 pKa = 11.84 TLLLEE260 pKa = 3.94 YY261 pKa = 10.26 CKK263 pKa = 10.74 KK264 pKa = 10.67 KK265 pKa = 10.43 ILAFRR270 pKa = 11.84 ADD272 pKa = 3.21 KK273 pKa = 10.99 NKK275 pKa = 10.39 RR276 pKa = 11.84 QTSSFEE282 pKa = 3.88 GMLVGAGGDD291 pKa = 3.77 LASVPAGRR299 pKa = 11.84 PAAGDD304 pKa = 3.67 LLPRR308 pKa = 11.84 ARR310 pKa = 11.84 GISPPPAATAGVFRR324 pKa = 11.84 VEE326 pKa = 4.38 QPRR329 pKa = 11.84 QPRR332 pKa = 11.84 RR333 pKa = 11.84 VRR335 pKa = 11.84 EE336 pKa = 3.99 EE337 pKa = 4.04 EE338 pKa = 3.94 PAGAPAHH345 pKa = 6.39 SGTGSRR351 pKa = 11.84 NTRR354 pKa = 11.84 ARR356 pKa = 11.84 TRR358 pKa = 11.84 QQHH361 pKa = 4.95 NEE363 pKa = 3.7 EE364 pKa = 4.67 SEE366 pKa = 4.32 VEE368 pKa = 4.25 SLRR371 pKa = 11.84 EE372 pKa = 3.65 FPGRR376 pKa = 11.84 ARR378 pKa = 11.84 QGNPPQPLL386 pKa = 3.25
Molecular weight: 42.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.038
IPC2_protein 7.483
IPC_protein 7.41
Toseland 7.088
ProMoST 7.922
Dawson 8.112
Bjellqvist 8.375
Wikipedia 8.039
Rodwell 8.126
Grimsley 7.102
Solomon 8.214
Lehninger 8.229
Nozaki 8.565
DTASelect 8.185
Thurlkill 8.244
EMBOSS 8.331
Sillero 8.551
Patrickios 4.571
IPC_peptide 8.214
IPC2_peptide 7.337
IPC2.peptide.svr19 7.592
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1440
386
1054
720.0
81.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.958 ± 1.641
1.806 ± 0.128
5.833 ± 0.332
7.5 ± 0.535
4.653 ± 0.127
7.917 ± 0.058
1.806 ± 0.26
3.472 ± 0.582
5.694 ± 0.399
10.208 ± 0.846
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.569 ± 0.385
2.847 ± 0.131
5.0 ± 0.357
3.889 ± 0.527
8.264 ± 0.119
5.0 ± 0.172
3.611 ± 0.404
6.042 ± 0.174
1.667 ± 0.718
3.264 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here