Capybara microvirus Cap1_SP_131
Average proteome isoelectric point is 4.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7U5|A0A4P8W7U5_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_131 OX=2585382 PE=4 SV=1
MM1 pKa = 7.76 FDD3 pKa = 4.1 VDD5 pKa = 4.45 DD6 pKa = 5.07 LNIFRR11 pKa = 11.84 PFFHH15 pKa = 7.56 DD16 pKa = 5.89 AILEE20 pKa = 4.26 SSGDD24 pKa = 3.67 VYY26 pKa = 11.31 SWSDD30 pKa = 3.47 LSLGAPLLPDD40 pKa = 4.36 TILNAVCFKK49 pKa = 10.67 VSEE52 pKa = 4.2 RR53 pKa = 11.84 LVSRR57 pKa = 11.84 FLMLLRR63 pKa = 11.84 SGFNCYY69 pKa = 9.88 FDD71 pKa = 4.54 LSYY74 pKa = 11.36 DD75 pKa = 3.98 FSPARR80 pKa = 11.84 DD81 pKa = 3.63 DD82 pKa = 4.23 FDD84 pKa = 4.59 FFFMVEE90 pKa = 4.17 PLCADD95 pKa = 3.44 DD96 pKa = 5.66 CDD98 pKa = 5.24 IVLSWLIKK106 pKa = 9.8 YY107 pKa = 9.57 CFLKK111 pKa = 10.98 VKK113 pKa = 9.97 EE114 pKa = 4.12
Molecular weight: 13.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.996
IPC2_protein 4.05
IPC_protein 4.037
Toseland 3.795
ProMoST 4.164
Dawson 4.037
Bjellqvist 4.253
Wikipedia 4.012
Rodwell 3.859
Grimsley 3.706
Solomon 4.024
Lehninger 3.986
Nozaki 4.151
DTASelect 4.444
Thurlkill 3.872
EMBOSS 4.012
Sillero 4.151
Patrickios 2.931
IPC_peptide 4.024
IPC2_peptide 4.126
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|A0A4P8W481|A0A4P8W481_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_131 OX=2585382 PE=3 SV=1
MM1 pKa = 7.04 FTTFKK6 pKa = 10.61 KK7 pKa = 10.52 DD8 pKa = 3.01 EE9 pKa = 4.22 YY10 pKa = 11.02 DD11 pKa = 3.67 FLVPEE16 pKa = 4.1 YY17 pKa = 10.68 AFNRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 QLFPRR28 pKa = 11.84 ADD30 pKa = 3.65 LPGLVNPIDD39 pKa = 4.08 YY40 pKa = 10.35 EE41 pKa = 4.09 NSQLDD46 pKa = 3.44 LSIPCRR52 pKa = 11.84 TCIGCRR58 pKa = 11.84 LDD60 pKa = 5.38 QSRR63 pKa = 11.84 QWADD67 pKa = 2.63 RR68 pKa = 11.84 MMIEE72 pKa = 5.16 LDD74 pKa = 3.3 HH75 pKa = 6.63 SKK77 pKa = 10.95 CGIFVTLTYY86 pKa = 10.75 DD87 pKa = 3.19 PAFAHH92 pKa = 6.76 PCSIDD97 pKa = 3.27 SDD99 pKa = 3.8 DD100 pKa = 3.59 RR101 pKa = 11.84 VYY103 pKa = 11.56 YY104 pKa = 10.75 SLDD107 pKa = 3.31 VRR109 pKa = 11.84 DD110 pKa = 3.63 TQLFNKK116 pKa = 9.26 SLRR119 pKa = 11.84 KK120 pKa = 9.82 AFPDD124 pKa = 3.07 RR125 pKa = 11.84 KK126 pKa = 9.97 IRR128 pKa = 11.84 FFLAGEE134 pKa = 4.22 YY135 pKa = 10.81 GPSTWRR141 pKa = 11.84 PHH143 pKa = 3.84 YY144 pKa = 9.6 HH145 pKa = 6.07 AVYY148 pKa = 10.06 FGLSIDD154 pKa = 4.72 DD155 pKa = 5.26 FPDD158 pKa = 3.76 LMPLKK163 pKa = 9.2 RR164 pKa = 11.84 TSAGSLIYY172 pKa = 10.23 TSEE175 pKa = 4.15 KK176 pKa = 10.18 LQKK179 pKa = 8.78 LWKK182 pKa = 9.88 RR183 pKa = 11.84 GFISLSPVSWQTCAYY198 pKa = 8.04 VARR201 pKa = 11.84 YY202 pKa = 5.27 TTKK205 pKa = 10.96 KK206 pKa = 10.74 LIDD209 pKa = 4.27 SNPLMSDD216 pKa = 3.0 YY217 pKa = 11.48 DD218 pKa = 4.4 LFNMHH223 pKa = 7.43 PEE225 pKa = 3.91 FMTCSRR231 pKa = 11.84 KK232 pKa = 9.69 PGLSGYY238 pKa = 10.31 FYY240 pKa = 10.56 DD241 pKa = 4.79 DD242 pKa = 4.41 HH243 pKa = 7.5 PDD245 pKa = 3.63 LVPDD249 pKa = 4.94 SIDD252 pKa = 3.3 DD253 pKa = 3.7 FKK255 pKa = 11.36 IYY257 pKa = 10.27 IRR259 pKa = 11.84 DD260 pKa = 3.68 PDD262 pKa = 3.56 GLKK265 pKa = 10.08 PVKK268 pKa = 10.09 SVKK271 pKa = 8.75 TPKK274 pKa = 10.5 YY275 pKa = 10.33 LLKK278 pKa = 10.37 QLQAVNPLLYY288 pKa = 10.51 NEE290 pKa = 4.3 YY291 pKa = 10.63 VDD293 pKa = 3.7 MQRR296 pKa = 11.84 VSSEE300 pKa = 3.99 DD301 pKa = 3.39 SLLLKK306 pKa = 10.2 LQRR309 pKa = 11.84 TSLPEE314 pKa = 3.59 AEE316 pKa = 4.78 YY317 pKa = 10.51 ILSEE321 pKa = 4.33 EE322 pKa = 4.17 FTKK325 pKa = 10.91 QNSAKK330 pKa = 10.45 KK331 pKa = 10.45 LIRR334 pKa = 11.84 SDD336 pKa = 3.5 LL337 pKa = 3.76
Molecular weight: 39.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.369
IPC2_protein 6.44
IPC_protein 6.59
Toseland 6.561
ProMoST 7.015
Dawson 7.088
Bjellqvist 6.985
Wikipedia 7.059
Rodwell 7.073
Grimsley 6.634
Solomon 7.117
Lehninger 7.132
Nozaki 7.307
DTASelect 7.366
Thurlkill 7.395
EMBOSS 7.424
Sillero 7.497
Patrickios 4.05
IPC_peptide 7.117
IPC2_peptide 6.942
IPC2.peptide.svr19 6.915
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1493
82
570
248.8
27.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.167 ± 1.321
1.273 ± 0.45
7.77 ± 1.217
3.885 ± 0.381
5.894 ± 0.789
5.894 ± 1.034
1.741 ± 0.428
4.287 ± 0.362
3.952 ± 0.9
9.109 ± 0.987
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.626
5.023 ± 0.861
6.095 ± 0.422
3.215 ± 0.589
4.756 ± 0.627
10.181 ± 1.775
5.291 ± 0.92
5.76 ± 0.584
1.407 ± 0.395
5.023 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here