Acetobacter sp. KACC 21233
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2638 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C1YQA3|A0A5C1YQA3_9PROT LptF/LptG family permease OS=Acetobacter sp. KACC 21233 OX=2592655 GN=FLP30_09230 PE=4 SV=1
MM1 pKa = 7.75 AGHH4 pKa = 6.93 IPISQIVQVTPGVLAAGNGLNNLSGLIATTASAALAAGAVQSFASADD51 pKa = 3.49 AVGTAFGDD59 pKa = 3.53 TSMLYY64 pKa = 10.75 QMAKK68 pKa = 10.26 VYY70 pKa = 10.27 FSGYY74 pKa = 7.35 STAVMTPGTLYY85 pKa = 10.69 VGAIAASASGATATASLGTVTEE107 pKa = 4.33 HH108 pKa = 5.71 TVATVAVDD116 pKa = 3.44 AAGTGYY122 pKa = 11.22 AVGDD126 pKa = 3.65 TASFSGGKK134 pKa = 8.15 ATISTVGTDD143 pKa = 3.81 GAVTALTLQSATAQSDD159 pKa = 3.84 DD160 pKa = 3.7 PAGTGLATTTNGSGTGLTVTTTSTAATVSSGAVSSITVNAGGAGYY205 pKa = 6.63 TTAPTVTLTGGGGTGAEE222 pKa = 4.08 ASATVSGGAVTAITVTAGGSGYY244 pKa = 9.24 TSAPAVTITAAASEE258 pKa = 4.36 DD259 pKa = 3.52 MAAQLSALRR268 pKa = 11.84 SANGGWNGLAFDD280 pKa = 5.13 AEE282 pKa = 4.39 LSVADD287 pKa = 4.39 KK288 pKa = 10.69 QAAAQWVSQQNGQIFAPLMDD308 pKa = 5.0 SDD310 pKa = 4.58 SGAATSGSDD319 pKa = 3.1 TAFGVWLGAQSISGVTAIYY338 pKa = 7.68 DD339 pKa = 3.86 TSPLSCALALGWMASLSFGTTDD361 pKa = 3.9 GRR363 pKa = 11.84 QTLAFVQDD371 pKa = 3.68 ASGLITPAVTDD382 pKa = 3.55 GTTAANLIANGYY394 pKa = 8.71 SFYY397 pKa = 11.22 GSYY400 pKa = 10.82 AGGADD405 pKa = 3.18 TFQFMRR411 pKa = 11.84 PGQVSGKK418 pKa = 9.05 FLWADD423 pKa = 3.37 SFVNQIWLNANLTSDD438 pKa = 5.15 LVNLLLNTGNIPYY451 pKa = 8.52 NTEE454 pKa = 3.42 GDD456 pKa = 4.07 TLVNAAVQDD465 pKa = 4.3 TITEE469 pKa = 4.09 ALAFGAIQAGVALTTLQKK487 pKa = 10.49 QQINNAAGVTTAADD501 pKa = 3.44 NVVNRR506 pKa = 11.84 GWYY509 pKa = 7.38 FQPNVSTAAASYY521 pKa = 9.83 RR522 pKa = 11.84 VARR525 pKa = 11.84 TTPPARR531 pKa = 11.84 LWYY534 pKa = 10.27 ADD536 pKa = 3.66 GEE538 pKa = 4.88 SVQSIQLNSVEE549 pKa = 4.35 VQQ551 pKa = 2.97
Molecular weight: 54.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.656
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.859
Rodwell 3.706
Grimsley 3.567
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.291
Thurlkill 3.719
EMBOSS 3.859
Sillero 4.012
Patrickios 0.693
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|A0A5C1YNM1|A0A5C1YNM1_9PROT ABC transporter permease subunit OS=Acetobacter sp. KACC 21233 OX=2592655 GN=FLP30_02880 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 ILGNRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.79 GRR39 pKa = 11.84 KK40 pKa = 8.87 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2638
0
2638
885515
26
1708
335.7
36.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.067 ± 0.069
1.063 ± 0.016
5.419 ± 0.039
4.985 ± 0.048
3.407 ± 0.031
8.307 ± 0.044
2.434 ± 0.021
4.608 ± 0.036
2.752 ± 0.038
10.572 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.509 ± 0.024
2.613 ± 0.031
5.59 ± 0.044
3.983 ± 0.031
6.936 ± 0.054
5.741 ± 0.038
5.984 ± 0.039
7.398 ± 0.047
1.394 ± 0.021
2.239 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here