Badger associated gemykibivirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus badas1

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3JHI0|A0A0E3JHI0_9VIRU Capsid OS=Badger associated gemykibivirus 1 OX=1634484 PE=4 SV=1
MM1 pKa = 7.49PAFYY5 pKa = 11.09VNSRR9 pKa = 11.84YY10 pKa = 10.25VLLTYY15 pKa = 10.05AQSGSLDD22 pKa = 3.52PFDD25 pKa = 5.26VVDD28 pKa = 4.55HH29 pKa = 6.55LASIHH34 pKa = 5.87GEE36 pKa = 4.06CIVGRR41 pKa = 11.84EE42 pKa = 3.96HH43 pKa = 7.09HH44 pKa = 7.01RR45 pKa = 11.84DD46 pKa = 3.52GGVHH50 pKa = 5.78LHH52 pKa = 6.47AFVHH56 pKa = 6.57FGRR59 pKa = 11.84KK60 pKa = 8.2FRR62 pKa = 11.84STSATVFDD70 pKa = 4.03VAGFHH75 pKa = 6.72PNISPSFGNPGRR87 pKa = 11.84GFDD90 pKa = 3.79YY91 pKa = 10.7AIKK94 pKa = 10.9DD95 pKa = 3.52GDD97 pKa = 3.5ICAGGLEE104 pKa = 4.21RR105 pKa = 11.84PGDD108 pKa = 3.78AGAPPTKK115 pKa = 10.17DD116 pKa = 2.73VWSQIVMAKK125 pKa = 10.43DD126 pKa = 3.44RR127 pKa = 11.84DD128 pKa = 4.24EE129 pKa = 4.36FWSLLRR135 pKa = 11.84QHH137 pKa = 6.17QPRR140 pKa = 11.84ALATSFVSLQKK151 pKa = 10.37YY152 pKa = 9.71ADD154 pKa = 2.82WHH156 pKa = 5.94FRR158 pKa = 11.84VDD160 pKa = 3.72PEE162 pKa = 4.77PYY164 pKa = 9.87QHH166 pKa = 7.18DD167 pKa = 4.08PSLGFYY173 pKa = 10.59LDD175 pKa = 5.14DD176 pKa = 4.17YY177 pKa = 10.79PEE179 pKa = 4.63LGSWVDD185 pKa = 3.91GYY187 pKa = 11.67LRR189 pKa = 11.84TNNTGMCAQSALSGLSGVGMSLVIYY214 pKa = 10.09GPSRR218 pKa = 11.84VGKK221 pKa = 6.67TVWARR226 pKa = 11.84SLGNHH231 pKa = 7.08AYY233 pKa = 10.43FGGLYY238 pKa = 10.49SLDD241 pKa = 3.48EE242 pKa = 4.37GVEE245 pKa = 4.12NVDD248 pKa = 3.31YY249 pKa = 11.29AVFDD253 pKa = 5.21DD254 pKa = 3.97IQGGLEE260 pKa = 3.85FFHH263 pKa = 7.45AYY265 pKa = 9.59KK266 pKa = 10.43FWLGHH271 pKa = 4.83QSQFYY276 pKa = 9.09ATDD279 pKa = 3.33KK280 pKa = 10.95YY281 pKa = 10.99KK282 pKa = 10.75SKK284 pKa = 11.11KK285 pKa = 8.89LIHH288 pKa = 6.74WGRR291 pKa = 11.84PAIWLANTDD300 pKa = 3.29PRR302 pKa = 11.84LEE304 pKa = 4.43KK305 pKa = 10.81GADD308 pKa = 3.83SDD310 pKa = 3.83WLEE313 pKa = 4.08ANCIFVCVTTPLYY326 pKa = 10.81SCQQ329 pKa = 3.41

Molecular weight: 36.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3JHI0|A0A0E3JHI0_9VIRU Capsid OS=Badger associated gemykibivirus 1 OX=1634484 PE=4 SV=1
MM1 pKa = 7.65ALRR4 pKa = 11.84YY5 pKa = 9.44KK6 pKa = 10.58SRR8 pKa = 11.84SNYY11 pKa = 9.01RR12 pKa = 11.84RR13 pKa = 11.84IPRR16 pKa = 11.84SRR18 pKa = 11.84AKK20 pKa = 9.76YY21 pKa = 8.68RR22 pKa = 11.84ARR24 pKa = 11.84KK25 pKa = 9.17RR26 pKa = 11.84SAPRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84TYY34 pKa = 10.68RR35 pKa = 11.84KK36 pKa = 8.15RR37 pKa = 11.84QMSVRR42 pKa = 11.84RR43 pKa = 11.84IRR45 pKa = 11.84NITSRR50 pKa = 11.84KK51 pKa = 8.61CQDD54 pKa = 2.69NMMPITISDD63 pKa = 3.82DD64 pKa = 3.37GVTEE68 pKa = 4.16GTPGVGVTMVGDD80 pKa = 3.97FVYY83 pKa = 9.36TFCFVPSGRR92 pKa = 11.84MPGPQYY98 pKa = 10.58GASEE102 pKa = 4.18RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84ARR107 pKa = 11.84TFARR111 pKa = 11.84GYY113 pKa = 10.65KK114 pKa = 8.71EE115 pKa = 3.99TGLLEE120 pKa = 4.37TQTDD124 pKa = 3.97AQWIWRR130 pKa = 11.84RR131 pKa = 11.84IVFRR135 pKa = 11.84MRR137 pKa = 11.84LGKK140 pKa = 9.55WIGGFDD146 pKa = 3.64TLLDD150 pKa = 3.77PTQTLILDD158 pKa = 3.68HH159 pKa = 6.26TTNGMMRR166 pKa = 11.84TLWNLGNTTDD176 pKa = 5.11LNAIDD181 pKa = 5.53RR182 pKa = 11.84NDD184 pKa = 3.93ALDD187 pKa = 4.44KK188 pKa = 10.95ILFKK192 pKa = 10.43GTQGQDD198 pKa = 2.57WADD201 pKa = 3.52KK202 pKa = 8.92FTAPVDD208 pKa = 3.63PTSVYY213 pKa = 10.76LIRR216 pKa = 11.84DD217 pKa = 3.34KK218 pKa = 10.91TRR220 pKa = 11.84YY221 pKa = 9.44IGGSMAASGRR231 pKa = 11.84FHH233 pKa = 7.73RR234 pKa = 11.84VRR236 pKa = 11.84DD237 pKa = 3.88WYY239 pKa = 10.01PVNRR243 pKa = 11.84TLVYY247 pKa = 10.53EE248 pKa = 4.32DD249 pKa = 4.69TEE251 pKa = 4.57HH252 pKa = 6.55GQPPDD257 pKa = 3.31TATPWSDD264 pKa = 3.63EE265 pKa = 3.87SRR267 pKa = 11.84TTCGDD272 pKa = 3.54VVVLDD277 pKa = 3.93MFRR280 pKa = 11.84CASGDD285 pKa = 3.49VTDD288 pKa = 4.37TMSFSPEE295 pKa = 3.23GCYY298 pKa = 9.88YY299 pKa = 9.39WHH301 pKa = 7.15EE302 pKa = 4.13

Molecular weight: 34.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

631

302

329

315.5

35.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.498 ± 0.814

1.743 ± 0.059

7.924 ± 0.016

3.17 ± 0.129

4.913 ± 0.862

9.033 ± 0.737

3.011 ± 1.145

3.803 ± 0.34

3.645 ± 0.002

6.815 ± 1.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.976

2.853 ± 0.087

5.071 ± 0.071

3.17 ± 0.129

8.558 ± 2.732

6.656 ± 0.697

6.815 ± 2.342

6.339 ± 0.482

2.694 ± 0.031

4.754 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski