Arrabida virus
Average proteome isoelectric point is 8.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A125QXV5|A0A125QXV5_9VIRU Nucleocapsid protein OS=Arrabida virus OX=1457322 GN=NP PE=3 SV=1
MM1 pKa = 7.54 FYY3 pKa = 11.2 AKK5 pKa = 10.37 FILLLCFVQGALLKK19 pKa = 8.9 YY20 pKa = 8.87 TIISKK25 pKa = 10.39 VGSSTYY31 pKa = 10.67 KK32 pKa = 10.08 RR33 pKa = 11.84 VCLSHH38 pKa = 6.2 TTDD41 pKa = 5.1 LDD43 pKa = 3.68 TLLDD47 pKa = 3.57 MTSRR51 pKa = 11.84 EE52 pKa = 3.86 MSNIKK57 pKa = 8.91 LTGGSCSLGDD67 pKa = 3.89 LTKK70 pKa = 10.16 PDD72 pKa = 4.14 CSPDD76 pKa = 3.23 FFSNYY81 pKa = 7.52 VANIQSSNTKK91 pKa = 8.5 ITGKK95 pKa = 9.69 FQDD98 pKa = 3.64 IIGEE102 pKa = 4.16 RR103 pKa = 11.84 EE104 pKa = 3.89 MEE106 pKa = 4.23 VTNDD110 pKa = 3.4 VVPDD114 pKa = 3.66 SFGPGPHH121 pKa = 7.13 DD122 pKa = 4.02 CVSFDD127 pKa = 5.38 KK128 pKa = 11.2 KK129 pKa = 10.78 PFWSDD134 pKa = 3.34 LLPTSVSKK142 pKa = 11.09 EE143 pKa = 3.85 EE144 pKa = 4.02 PKK146 pKa = 10.64 PIISTLSVLPAAPPIKK162 pKa = 10.33 DD163 pKa = 3.66 FSHH166 pKa = 6.34 KK167 pKa = 10.33 VVEE170 pKa = 4.18 AEE172 pKa = 4.08 EE173 pKa = 4.09 MVKK176 pKa = 10.39 EE177 pKa = 4.23 IEE179 pKa = 4.21 GEE181 pKa = 4.01 LMKK184 pKa = 10.84 EE185 pKa = 4.14 KK186 pKa = 10.35 EE187 pKa = 4.06 RR188 pKa = 11.84 NSAKK192 pKa = 10.41 EE193 pKa = 3.58 EE194 pKa = 3.81 EE195 pKa = 4.27 ARR197 pKa = 11.84 QRR199 pKa = 11.84 MRR201 pKa = 11.84 EE202 pKa = 4.1 LEE204 pKa = 4.51 DD205 pKa = 4.23 DD206 pKa = 5.18 LRR208 pKa = 11.84 KK209 pKa = 9.6 QWAAEE214 pKa = 3.9 RR215 pKa = 11.84 MAKK218 pKa = 10.28 QNMSSVLEE226 pKa = 4.06 RR227 pKa = 11.84 EE228 pKa = 4.18 RR229 pKa = 11.84 QEE231 pKa = 4.04 KK232 pKa = 10.15 QSLEE236 pKa = 3.95 RR237 pKa = 11.84 RR238 pKa = 11.84 MHH240 pKa = 6.13 EE241 pKa = 3.67 VDD243 pKa = 2.96 KK244 pKa = 10.96 KK245 pKa = 11.0 LRR247 pKa = 11.84 ASEE250 pKa = 4.82 DD251 pKa = 3.21 RR252 pKa = 11.84 AQKK255 pKa = 8.44 YY256 pKa = 7.12 QEE258 pKa = 3.97 RR259 pKa = 11.84 AEE261 pKa = 4.12 QAEE264 pKa = 4.34 KK265 pKa = 10.77 SHH267 pKa = 6.52 SKK269 pKa = 10.45 LLSRR273 pKa = 11.84 SGAATTMATVVFTSLLASGLAVVTEE298 pKa = 4.05 LEE300 pKa = 4.41 KK301 pKa = 10.72 RR302 pKa = 11.84 DD303 pKa = 3.93 TNHH306 pKa = 7.15 LLNRR310 pKa = 11.84 PGNGVYY316 pKa = 8.88 MANADD321 pKa = 3.79 SLIQTEE327 pKa = 4.2 CTLNYY332 pKa = 9.91 GQRR335 pKa = 11.84 CLAWEE340 pKa = 4.04 LQITPVRR347 pKa = 11.84 YY348 pKa = 8.79 PFFTSNVDD356 pKa = 3.13 KK357 pKa = 11.34 YY358 pKa = 11.59 SLLEE362 pKa = 4.72 SITEE366 pKa = 4.12 TPSILEE372 pKa = 4.44 KK373 pKa = 11.15 NNEE376 pKa = 4.06 SCQLATTSGSLKK388 pKa = 9.16 TCAKK392 pKa = 9.09 EE393 pKa = 3.26 ASTIKK398 pKa = 10.23 KK399 pKa = 9.56 HH400 pKa = 5.78 CTDD403 pKa = 3.49 DD404 pKa = 3.19 VRR406 pKa = 11.84 AFFFINSVGKK416 pKa = 8.52 LTIVRR421 pKa = 11.84 CADD424 pKa = 3.2 NHH426 pKa = 6.6 ILSEE430 pKa = 4.33 DD431 pKa = 3.8 CNFCISKK438 pKa = 10.37 ARR440 pKa = 11.84 NSNQNMFMPVQDD452 pKa = 4.98 AFCQRR457 pKa = 11.84 QGSDD461 pKa = 2.95 TAPAIRR467 pKa = 11.84 YY468 pKa = 9.34 SKK470 pKa = 10.12 DD471 pKa = 2.7 ICSIGAIKK479 pKa = 10.29 IKK481 pKa = 9.94 QCHH484 pKa = 5.83 KK485 pKa = 9.42 ATSKK489 pKa = 10.16 FEE491 pKa = 4.07 RR492 pKa = 11.84 MGFISVGQKK501 pKa = 10.14 KK502 pKa = 10.43 LYY504 pKa = 10.14 IEE506 pKa = 4.12 EE507 pKa = 4.02 LKK509 pKa = 10.77 LRR511 pKa = 11.84 YY512 pKa = 9.03 RR513 pKa = 11.84 QEE515 pKa = 3.83 YY516 pKa = 10.96 DD517 pKa = 3.09 PDD519 pKa = 3.2 QFMCYY524 pKa = 9.48 KK525 pKa = 9.82 IKK527 pKa = 10.71 SSSPLKK533 pKa = 10.06 YY534 pKa = 10.31 EE535 pKa = 3.88 KK536 pKa = 10.79 VSSKK540 pKa = 10.34 KK541 pKa = 10.4 CKK543 pKa = 10.16 EE544 pKa = 3.56 VDD546 pKa = 2.77 TGGNQKK552 pKa = 10.42 CSGDD556 pKa = 3.3 EE557 pKa = 4.21 HH558 pKa = 6.41 FCNRR562 pKa = 11.84 YY563 pKa = 8.97 PCDD566 pKa = 3.3 TANPEE571 pKa = 4.1 AHH573 pKa = 6.8 CLTRR577 pKa = 11.84 KK578 pKa = 9.84 HH579 pKa = 5.85 SAIAEE584 pKa = 4.18 VNIGGVWIRR593 pKa = 11.84 PKK595 pKa = 10.41 CVGYY599 pKa = 11.04 EE600 pKa = 3.78 MVLVKK605 pKa = 10.54 RR606 pKa = 11.84 SGLKK610 pKa = 10.27 SEE612 pKa = 4.73 DD613 pKa = 3.45 LSIRR617 pKa = 11.84 DD618 pKa = 4.18 CPSCLWEE625 pKa = 4.4 CKK627 pKa = 9.71 KK628 pKa = 11.02 SRR630 pKa = 11.84 ITIKK634 pKa = 8.91 THH636 pKa = 4.71 GPKK639 pKa = 9.62 IVSATACSHH648 pKa = 6.42 GSCKK652 pKa = 10.54 SVMQKK657 pKa = 10.4 PSTFVEE663 pKa = 3.97 IPYY666 pKa = 9.59 PGNSEE671 pKa = 4.55 IIGGDD676 pKa = 2.71 IGVHH680 pKa = 3.87 MTEE683 pKa = 4.64 RR684 pKa = 11.84 GSPSNIHH691 pKa = 5.98 VRR693 pKa = 11.84 THH695 pKa = 6.23 CPPRR699 pKa = 11.84 DD700 pKa = 3.54 SCDD703 pKa = 3.33 VSDD706 pKa = 6.22 CMFCVHH712 pKa = 7.5 GILNYY717 pKa = 9.83 QCHH720 pKa = 5.56 TLASALTIATIVSAVLVVVYY740 pKa = 9.02 FLLARR745 pKa = 11.84 SKK747 pKa = 10.67 RR748 pKa = 11.84 AVKK751 pKa = 10.28 FITSLLIVPFKK762 pKa = 10.45 WLTLLSIWVVKK773 pKa = 8.77 NWKK776 pKa = 10.0 RR777 pKa = 11.84 KK778 pKa = 6.2 MSKK781 pKa = 10.21 LARR784 pKa = 11.84 ATNNAIGWDD793 pKa = 3.44 QDD795 pKa = 3.74 VEE797 pKa = 4.41 RR798 pKa = 11.84 GRR800 pKa = 11.84 PRR802 pKa = 11.84 AAAGHH807 pKa = 5.2 QNQVARR813 pKa = 11.84 YY814 pKa = 6.34 TFYY817 pKa = 11.27 GATILSLMTSGLCCTEE833 pKa = 3.99 SVIAEE838 pKa = 4.24 SNIMQCTTSDD848 pKa = 3.23 STTTCKK854 pKa = 10.76 ASGTIILRR862 pKa = 11.84 LGPIGSEE869 pKa = 3.66 SCLILKK875 pKa = 8.76 GLRR878 pKa = 11.84 DD879 pKa = 3.66 SEE881 pKa = 4.34 KK882 pKa = 9.99 QFISIRR888 pKa = 11.84 TVSSEE893 pKa = 3.93 LTCRR897 pKa = 11.84 EE898 pKa = 3.82 GDD900 pKa = 4.01 SFWTSLYY907 pKa = 10.6 TPICLSSRR915 pKa = 11.84 RR916 pKa = 11.84 CHH918 pKa = 7.36 LMGEE922 pKa = 4.53 CTGDD926 pKa = 4.18 NCLKK930 pKa = 10.07 WQDD933 pKa = 3.69 NKK935 pKa = 10.62 TSSEE939 pKa = 4.17 FTGRR943 pKa = 11.84 THH945 pKa = 8.41 SEE947 pKa = 3.71 VLHH950 pKa = 5.89 EE951 pKa = 4.49 NKK953 pKa = 10.16 CFEE956 pKa = 3.93 QSGGIGYY963 pKa = 9.83 GCFNVNPSCLMVHH976 pKa = 6.67 SYY978 pKa = 10.96 LKK980 pKa = 10.27 PIYY983 pKa = 10.18 KK984 pKa = 10.13 NAFKK988 pKa = 10.91 VFRR991 pKa = 11.84 CVAWNHH997 pKa = 4.89 RR998 pKa = 11.84 VKK1000 pKa = 10.88 LAITTHH1006 pKa = 6.09 KK1007 pKa = 10.65 KK1008 pKa = 10.6 SFDD1011 pKa = 3.55 VTLMAMSTQPTDD1023 pKa = 2.68 WGSIGLILDD1032 pKa = 3.72 SEE1034 pKa = 5.38 GITGTNSYY1042 pKa = 11.42 SFMKK1046 pKa = 10.65 YY1047 pKa = 10.13 GVGSFAIIDD1056 pKa = 3.82 EE1057 pKa = 4.58 PFSIEE1062 pKa = 3.8 PRR1064 pKa = 11.84 KK1065 pKa = 10.54 GFLGEE1070 pKa = 4.13 VRR1072 pKa = 11.84 CPTEE1076 pKa = 3.66 EE1077 pKa = 4.14 TAVKK1081 pKa = 10.43 ASPSCKK1087 pKa = 9.63 AAPQIIEE1094 pKa = 4.1 YY1095 pKa = 9.21 HH1096 pKa = 6.52 PEE1098 pKa = 3.71 MDD1100 pKa = 3.25 TVEE1103 pKa = 5.38 CITNMMDD1110 pKa = 3.78 PMAIFNRR1117 pKa = 11.84 GSLPQVRR1124 pKa = 11.84 DD1125 pKa = 3.23 GVTFAQSIEE1134 pKa = 4.3 KK1135 pKa = 10.69 NSVQALTTGEE1145 pKa = 3.85 IKK1147 pKa = 10.77 ASVRR1151 pKa = 11.84 LVLDD1155 pKa = 4.4 DD1156 pKa = 4.57 YY1157 pKa = 10.99 EE1158 pKa = 4.65 VEE1160 pKa = 4.45 YY1161 pKa = 10.77 KK1162 pKa = 10.83 VSEE1165 pKa = 4.12 NDD1167 pKa = 3.62 CDD1169 pKa = 3.75 STFINITGCYY1179 pKa = 9.56 SCDD1182 pKa = 2.99 EE1183 pKa = 4.23 GARR1186 pKa = 11.84 LCVRR1190 pKa = 11.84 IRR1192 pKa = 11.84 TLGDD1196 pKa = 3.47 SIYY1199 pKa = 10.62 HH1200 pKa = 5.27 FTDD1203 pKa = 3.23 SEE1205 pKa = 4.44 DD1206 pKa = 3.42 MMNVMFKK1213 pKa = 10.76 VNSSVTEE1220 pKa = 4.07 YY1221 pKa = 10.17 CTVLHH1226 pKa = 6.48 FSKK1229 pKa = 10.47 PVVNFEE1235 pKa = 4.03 GKK1237 pKa = 9.74 YY1238 pKa = 10.5 DD1239 pKa = 3.69 CGKK1242 pKa = 10.08 SRR1244 pKa = 11.84 KK1245 pKa = 9.19 PMIIKK1250 pKa = 8.61 GTLIAMAPHH1259 pKa = 7.86 DD1260 pKa = 4.63 DD1261 pKa = 3.84 RR1262 pKa = 11.84 VHH1264 pKa = 6.88 SGGSSIVVNPKK1275 pKa = 10.39 SKK1277 pKa = 10.72 GVDD1280 pKa = 3.82 FLGWLSGLSSWLGGPLKK1297 pKa = 10.05 TVLTVLGFLILGLVFVIVVIFLVRR1321 pKa = 11.84 VGIRR1325 pKa = 11.84 QALLKK1330 pKa = 10.58 KK1331 pKa = 10.0 MKK1333 pKa = 10.4
Molecular weight: 148.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.135
IPC2_protein 7.483
IPC_protein 7.22
Toseland 6.81
ProMoST 7.907
Dawson 7.951
Bjellqvist 8.492
Wikipedia 7.849
Rodwell 7.995
Grimsley 6.839
Solomon 7.966
Lehninger 7.995
Nozaki 8.814
DTASelect 8.112
Thurlkill 8.185
EMBOSS 8.17
Sillero 8.565
Patrickios 4.215
IPC_peptide 7.966
IPC2_peptide 7.527
IPC2.peptide.svr19 7.456
Protein with the highest isoelectric point:
>tr|A0A0X9U9D7|A0A0X9U9D7_9VIRU Glycoprotein OS=Arrabida virus OX=1457322 GN=GP PE=4 SV=1
MM1 pKa = 7.66 LSRR4 pKa = 11.84 VVYY7 pKa = 9.9 LRR9 pKa = 11.84 KK10 pKa = 9.73 RR11 pKa = 11.84 SSDD14 pKa = 3.14 RR15 pKa = 11.84 KK16 pKa = 10.09 KK17 pKa = 11.0 GRR19 pKa = 11.84 LQKK22 pKa = 10.67 RR23 pKa = 11.84 FFVDD27 pKa = 3.15 SFLNEE32 pKa = 3.59 AALFGPFSLAGGEE45 pKa = 4.49 VPFTVYY51 pKa = 10.59 KK52 pKa = 10.53 NSVVFDD58 pKa = 3.55 SRR60 pKa = 11.84 PTLNHH65 pKa = 5.81 YY66 pKa = 9.76 LIKK69 pKa = 10.96 NEE71 pKa = 4.05 FPAILSGEE79 pKa = 4.38 MINLPSTTLYY89 pKa = 10.54 NPIMRR94 pKa = 11.84 EE95 pKa = 4.07 LYY97 pKa = 10.09 QEE99 pKa = 4.87 SIHH102 pKa = 6.47 QIKK105 pKa = 10.39 RR106 pKa = 11.84 SNRR109 pKa = 11.84 DD110 pKa = 3.3 YY111 pKa = 11.17 LVSALRR117 pKa = 11.84 WPTGLASLEE126 pKa = 4.49 FIDD129 pKa = 6.96 FYY131 pKa = 11.58 FEE133 pKa = 5.09 DD134 pKa = 5.5 FIFLSVTDD142 pKa = 4.31 SYY144 pKa = 11.56 ATNKK148 pKa = 9.8 LLKK151 pKa = 10.51 LLMKK155 pKa = 10.86 ASGDD159 pKa = 3.55 QSCDD163 pKa = 2.65 IEE165 pKa = 4.35 KK166 pKa = 10.22 QIKK169 pKa = 9.42 IIYY172 pKa = 9.71 RR173 pKa = 11.84 KK174 pKa = 9.55 ILLEE178 pKa = 4.04 GSKK181 pKa = 10.71 FGMNCYY187 pKa = 9.91 HH188 pKa = 6.64 LTGADD193 pKa = 3.98 IIRR196 pKa = 11.84 DD197 pKa = 3.25 ICIIQSARR205 pKa = 11.84 VARR208 pKa = 11.84 LAQKK212 pKa = 6.86 TQKK215 pKa = 10.43 SPSRR219 pKa = 11.84 DD220 pKa = 3.52 VVRR223 pKa = 11.84 MIYY226 pKa = 10.24 QSLSPNDD233 pKa = 4.0 LLVSPKK239 pKa = 10.18 HH240 pKa = 6.3 SGVSTEE246 pKa = 3.97 APKK249 pKa = 10.32 LAEE252 pKa = 3.96 MSAEE256 pKa = 3.79 EE257 pKa = 4.09 SFNYY261 pKa = 9.6 FMAEE265 pKa = 3.78 DD266 pKa = 3.8 VEE268 pKa = 4.49 FRR270 pKa = 11.84 TMALSTFWTRR280 pKa = 11.84 DD281 pKa = 2.77 WPTAAEE287 pKa = 4.34 TVASLRR293 pKa = 11.84 QKK295 pKa = 10.0 SRR297 pKa = 11.84 RR298 pKa = 11.84 RR299 pKa = 11.84 VKK301 pKa = 10.2 KK302 pKa = 9.39 HH303 pKa = 5.79 ASALPVSPDD312 pKa = 3.43 YY313 pKa = 11.08 PPLL316 pKa = 5.2
Molecular weight: 36.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.845
IPC2_protein 8.873
IPC_protein 8.814
Toseland 9.443
ProMoST 9.253
Dawson 9.736
Bjellqvist 9.472
Wikipedia 9.926
Rodwell 9.97
Grimsley 9.823
Solomon 9.765
Lehninger 9.721
Nozaki 9.531
DTASelect 9.443
Thurlkill 9.56
EMBOSS 9.882
Sillero 9.677
Patrickios 4.965
IPC_peptide 9.765
IPC2_peptide 8.068
IPC2.peptide.svr19 7.878
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1905
256
1333
635.0
71.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.247 ± 1.074
3.202 ± 1.269
4.934 ± 0.185
6.089 ± 0.282
4.199 ± 0.493
5.564 ± 0.695
1.995 ± 0.445
6.247 ± 0.167
7.559 ± 0.519
8.294 ± 0.738
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.202 ± 0.651
3.832 ± 0.24
4.199 ± 0.474
2.887 ± 0.155
5.302 ± 0.634
9.291 ± 1.072
6.562 ± 0.634
6.509 ± 0.404
1.102 ± 0.049
2.782 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here