Flavonifractor sp. An10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Flavonifractor; unclassified Flavonifractor

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3501 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4WB73|A0A1Y4WB73_9FIRM Uncharacterized protein OS=Flavonifractor sp. An10 OX=1965537 GN=B5E42_16925 PE=4 SV=1
MM1 pKa = 7.05EE2 pKa = 4.52TFVSNFRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.57SKK14 pKa = 9.36MRR16 pKa = 11.84NLKK19 pKa = 8.77RR20 pKa = 11.84TLSLVLAVVMVIGLMVVGASAVSYY44 pKa = 11.34NDD46 pKa = 3.04FSDD49 pKa = 4.22RR50 pKa = 11.84EE51 pKa = 4.17EE52 pKa = 4.31IVNKK56 pKa = 10.34DD57 pKa = 3.57AVSMLTTLGIIEE69 pKa = 4.31GQPDD73 pKa = 3.75GSYY76 pKa = 11.27NPTGDD81 pKa = 3.1VDD83 pKa = 3.94RR84 pKa = 11.84AQMAKK89 pKa = 9.16MISVALTNNEE99 pKa = 4.29DD100 pKa = 4.21CDD102 pKa = 4.02TLYY105 pKa = 11.16TNVNSGLTDD114 pKa = 3.46ITANWARR121 pKa = 11.84GYY123 pKa = 10.58INYY126 pKa = 9.77CYY128 pKa = 10.64VRR130 pKa = 11.84GIIAGRR136 pKa = 11.84GDD138 pKa = 3.45NTFDD142 pKa = 3.89PDD144 pKa = 3.84ANVTGVEE151 pKa = 3.96AAKK154 pKa = 9.98MLLAALGYY162 pKa = 10.05NADD165 pKa = 3.52IEE167 pKa = 4.77GLVGPDD173 pKa = 2.83WALNTAALAQQLGIFRR189 pKa = 11.84NFTKK193 pKa = 10.53DD194 pKa = 2.98VSEE197 pKa = 4.12PLSRR201 pKa = 11.84DD202 pKa = 3.27DD203 pKa = 5.17AALLIYY209 pKa = 10.43NALDD213 pKa = 3.21VEE215 pKa = 5.09MIQQYY220 pKa = 6.04QTGYY224 pKa = 8.82ALVYY228 pKa = 10.15ADD230 pKa = 4.58HH231 pKa = 6.62RR232 pKa = 11.84TILSSVFGVVRR243 pKa = 11.84VEE245 pKa = 4.35GVVTANEE252 pKa = 3.92WARR255 pKa = 11.84LEE257 pKa = 4.26EE258 pKa = 4.25TDD260 pKa = 3.79SDD262 pKa = 3.86AALRR266 pKa = 11.84TGKK269 pKa = 7.28TTLDD273 pKa = 3.37EE274 pKa = 4.35VVVYY278 pKa = 10.76DD279 pKa = 3.84STTSNTTVPEE289 pKa = 5.11GIRR292 pKa = 11.84EE293 pKa = 4.16DD294 pKa = 4.12DD295 pKa = 3.45PVTFNVSTPVDD306 pKa = 3.93MIGKK310 pKa = 9.0AVTMYY315 pKa = 10.71VEE317 pKa = 4.27KK318 pKa = 8.58TTILANSNVIGIATNDD334 pKa = 3.52EE335 pKa = 4.41ANVIHH340 pKa = 6.46ATAATEE346 pKa = 4.03EE347 pKa = 4.31TLDD350 pKa = 4.72DD351 pKa = 4.72LLDD354 pKa = 3.88GTGIEE359 pKa = 4.47TDD361 pKa = 3.65RR362 pKa = 11.84NTQYY366 pKa = 10.89YY367 pKa = 9.68VNYY370 pKa = 9.16GWCEE374 pKa = 3.94DD375 pKa = 3.66GATEE379 pKa = 4.81AVEE382 pKa = 6.05LINQYY387 pKa = 10.75PDD389 pKa = 3.08AQLEE393 pKa = 4.37DD394 pKa = 4.56KK395 pKa = 11.32DD396 pKa = 3.63PGYY399 pKa = 11.27YY400 pKa = 10.14SLNGVEE406 pKa = 5.64VEE408 pKa = 4.9VIDD411 pKa = 5.16NNDD414 pKa = 3.74DD415 pKa = 3.42GTAEE419 pKa = 4.08YY420 pKa = 11.09VLYY423 pKa = 10.5LQEE426 pKa = 4.25TLSEE430 pKa = 4.15VTRR433 pKa = 11.84YY434 pKa = 9.8SDD436 pKa = 3.52RR437 pKa = 11.84NEE439 pKa = 4.19TISFYY444 pKa = 10.59IPEE447 pKa = 4.98LNNSGAPTGDD457 pKa = 3.26SDD459 pKa = 4.03TVTRR463 pKa = 11.84DD464 pKa = 3.28FEE466 pKa = 5.03DD467 pKa = 4.11VVFNDD472 pKa = 5.05DD473 pKa = 3.35VAADD477 pKa = 3.92DD478 pKa = 4.97LILYY482 pKa = 9.36VEE484 pKa = 4.38YY485 pKa = 10.75GGRR488 pKa = 11.84TYY490 pKa = 10.64IQLAPIVTDD499 pKa = 2.87TMNRR503 pKa = 11.84VDD505 pKa = 4.23RR506 pKa = 11.84DD507 pKa = 3.63RR508 pKa = 11.84DD509 pKa = 3.57SEE511 pKa = 4.9LYY513 pKa = 8.65ITLDD517 pKa = 2.83NGEE520 pKa = 4.91EE521 pKa = 3.92YY522 pKa = 10.41RR523 pKa = 11.84QAYY526 pKa = 9.64LPDD529 pKa = 4.31AASMTDD535 pKa = 3.16VEE537 pKa = 4.83LTHH540 pKa = 7.25FDD542 pKa = 3.32IDD544 pKa = 3.6NARR547 pKa = 11.84ADD549 pKa = 3.26IGFDD553 pKa = 3.42DD554 pKa = 5.68AYY556 pKa = 11.21DD557 pKa = 4.89FILDD561 pKa = 3.44ATEE564 pKa = 4.02QYY566 pKa = 10.82VIAVRR571 pKa = 11.84PAEE574 pKa = 4.11EE575 pKa = 3.85QVTNYY580 pKa = 11.27ALVLGSAWTQNALTRR595 pKa = 11.84SGEE598 pKa = 4.22VKK600 pKa = 10.17ILMADD605 pKa = 3.5GTEE608 pKa = 3.91NTFDD612 pKa = 3.85IDD614 pKa = 3.35WNEE617 pKa = 3.74SRR619 pKa = 11.84KK620 pKa = 9.83VFEE623 pKa = 4.79NEE625 pKa = 3.7TTNTRR630 pKa = 11.84KK631 pKa = 8.78DD632 pKa = 3.43TALEE636 pKa = 4.25NYY638 pKa = 10.17LGTRR642 pKa = 11.84DD643 pKa = 3.77VQHH646 pKa = 6.48SGSYY650 pKa = 8.36RR651 pKa = 11.84TGAAVGTVIEE661 pKa = 4.54YY662 pKa = 10.77SLNDD666 pKa = 3.84AGDD669 pKa = 3.54EE670 pKa = 4.01LTIEE674 pKa = 4.86NILNLNTLADD684 pKa = 3.82GGYY687 pKa = 10.27VNQNNGDD694 pKa = 3.99PLVDD698 pKa = 3.67VVSNPIAYY706 pKa = 9.19IAANRR711 pKa = 11.84AASDD715 pKa = 3.56TSVRR719 pKa = 11.84TANNLQYY726 pKa = 10.29TIQTGQPYY734 pKa = 8.59STGDD738 pKa = 2.99GTLYY742 pKa = 9.96LTVKK746 pKa = 9.32NTDD749 pKa = 2.98AVADD753 pKa = 3.87ARR755 pKa = 11.84NDD757 pKa = 3.58NASFAIDD764 pKa = 3.5RR765 pKa = 11.84DD766 pKa = 4.19TIAFYY771 pKa = 11.11YY772 pKa = 10.28VDD774 pKa = 3.56SDD776 pKa = 4.12TYY778 pKa = 11.47GVATGYY784 pKa = 11.39SNMSDD789 pKa = 3.41IPVQDD794 pKa = 3.45EE795 pKa = 4.19DD796 pKa = 4.34GNPVSVQVYY805 pKa = 8.97PVLEE809 pKa = 4.31KK810 pKa = 11.01NNQGGWTASRR820 pKa = 11.84LADD823 pKa = 3.21VALFNSEE830 pKa = 4.44PSNDD834 pKa = 3.63SNDD837 pKa = 3.51YY838 pKa = 10.41MLVLNANYY846 pKa = 10.2YY847 pKa = 10.59RR848 pKa = 11.84GDD850 pKa = 3.97EE851 pKa = 3.89LWLNVVFEE859 pKa = 5.65DD860 pKa = 3.99GTAATIEE867 pKa = 3.92IDD869 pKa = 4.63DD870 pKa = 4.85DD871 pKa = 4.27GGHH874 pKa = 5.97NFDD877 pKa = 4.09RR878 pKa = 11.84TSSYY882 pKa = 9.36MKK884 pKa = 10.15AYY886 pKa = 10.25AYY888 pKa = 10.63VEE890 pKa = 4.4NADD893 pKa = 3.46GTYY896 pKa = 10.19DD897 pKa = 3.48ISDD900 pKa = 3.7RR901 pKa = 11.84DD902 pKa = 3.92PIGEE906 pKa = 4.06YY907 pKa = 10.08DD908 pKa = 3.97AHH910 pKa = 6.04RR911 pKa = 11.84TDD913 pKa = 3.7NGTIYY918 pKa = 9.88WAAPTSVSPNPYY930 pKa = 8.74LTLLDD935 pKa = 4.41SSNVWDD941 pKa = 3.89VTDD944 pKa = 3.65VDD946 pKa = 4.6SANDD950 pKa = 4.67DD951 pKa = 3.28ITAGTFTRR959 pKa = 11.84TDD961 pKa = 3.16KK962 pKa = 11.19NAVIVTGGSRR972 pKa = 11.84DD973 pKa = 3.55EE974 pKa = 4.5TIRR977 pKa = 11.84AAWIWDD983 pKa = 3.39IDD985 pKa = 3.92DD986 pKa = 3.83TTVDD990 pKa = 4.88FDD992 pKa = 3.72TSDD995 pKa = 3.76YY996 pKa = 11.42LVVLSDD1002 pKa = 4.87ADD1004 pKa = 3.48NCAYY1008 pKa = 9.69IYY1010 pKa = 11.27YY1011 pKa = 10.35NDD1013 pKa = 5.06DD1014 pKa = 4.5LYY1016 pKa = 11.41DD1017 pKa = 4.16SPDD1020 pKa = 3.67FPDD1023 pKa = 5.5LDD1025 pKa = 3.9LCMSLIEE1032 pKa = 5.98AEE1034 pKa = 4.69MEE1036 pKa = 4.07DD1037 pKa = 3.24QGYY1040 pKa = 8.55TIVDD1044 pKa = 3.44RR1045 pKa = 11.84TRR1047 pKa = 11.84NGNTYY1052 pKa = 7.26TWEE1055 pKa = 4.19VEE1057 pKa = 4.11DD1058 pKa = 5.26SRR1060 pKa = 11.84GFDD1063 pKa = 3.0DD1064 pKa = 4.52TYY1066 pKa = 11.36RR1067 pKa = 11.84FVYY1070 pKa = 10.31DD1071 pKa = 3.33ATVSFTGNWLIPSYY1085 pKa = 10.38VGRR1088 pKa = 11.84VNGVEE1093 pKa = 4.23TFVPTASATITNRR1106 pKa = 11.84TDD1108 pKa = 2.9SSTHH1112 pKa = 5.87LAGSTPLAGTHH1123 pKa = 3.83VWGRR1127 pKa = 11.84LDD1129 pKa = 4.55ANNNYY1134 pKa = 10.4DD1135 pKa = 3.99WIAVGSVTVTGSGFDD1150 pKa = 3.37IEE1152 pKa = 5.17DD1153 pKa = 4.31GYY1155 pKa = 9.56TAVDD1159 pKa = 3.62VAGLALSITTDD1170 pKa = 2.89GDD1172 pKa = 3.67LDD1174 pKa = 3.8VAVTVNGDD1182 pKa = 3.71DD1183 pKa = 6.26LEE1185 pKa = 6.51DD1186 pKa = 4.98CDD1188 pKa = 5.22TYY1190 pKa = 11.66DD1191 pKa = 3.22AANDD1195 pKa = 3.54VVYY1198 pKa = 11.21VEE1200 pKa = 5.57GNDD1203 pKa = 3.51NEE1205 pKa = 4.38IVATVTGSFEE1215 pKa = 3.7ITTASDD1221 pKa = 3.06TKK1223 pKa = 11.07YY1224 pKa = 10.46IAGTNTNGLVRR1235 pKa = 11.84DD1236 pKa = 3.78VAGYY1240 pKa = 9.38DD1241 pKa = 3.31ASRR1244 pKa = 11.84NNATVTTPTGSGQIVFTASADD1265 pKa = 3.53GATTFEE1271 pKa = 4.34DD1272 pKa = 4.08VEE1274 pKa = 4.87VTFTWTVDD1282 pKa = 3.52QQTTTVDD1289 pKa = 3.46LNLATDD1295 pKa = 4.26LTT1297 pKa = 4.35

Molecular weight: 141.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4WG85|A0A1Y4WG85_9FIRM Uncharacterized protein OS=Flavonifractor sp. An10 OX=1965537 GN=B5E42_12325 PE=4 SV=1
MM1 pKa = 7.06VRR3 pKa = 11.84TYY5 pKa = 10.22QPKK8 pKa = 9.24KK9 pKa = 7.68RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.13GFRR19 pKa = 11.84KK20 pKa = 9.96RR21 pKa = 11.84MRR23 pKa = 11.84TANGRR28 pKa = 11.84KK29 pKa = 8.55VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.28

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3501

0

3501

1082429

37

1867

309.2

34.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.147 ± 0.06

1.571 ± 0.017

5.609 ± 0.038

6.998 ± 0.043

3.683 ± 0.027

8.162 ± 0.04

1.851 ± 0.019

5.089 ± 0.044

4.165 ± 0.042

10.457 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.02

3.042 ± 0.03

4.438 ± 0.031

3.479 ± 0.025

6.274 ± 0.048

5.253 ± 0.03

5.443 ± 0.038

7.134 ± 0.038

1.185 ± 0.017

3.468 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski