Cyanophage S-RIM12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Brizovirus; unclassified Brizovirus

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D7SZ94|A0A1D7SZ94_9CAUD Neck protein OS=Cyanophage S-RIM12 OX=1278402 GN=Sn070910_093 PE=4 SV=1
MM1 pKa = 7.59FVLTDD6 pKa = 3.55INSGGIYY13 pKa = 10.38AVTSKK18 pKa = 11.32DD19 pKa = 3.43SIKK22 pKa = 9.99TVTVFEE28 pKa = 5.06DD29 pKa = 3.36RR30 pKa = 11.84DD31 pKa = 3.61DD32 pKa = 3.99AEE34 pKa = 5.45RR35 pKa = 11.84YY36 pKa = 8.97VQLLLADD43 pKa = 4.71DD44 pKa = 4.71YY45 pKa = 11.75EE46 pKa = 6.25DD47 pKa = 3.45EE48 pKa = 4.96LEE50 pKa = 4.1IFEE53 pKa = 4.18VDD55 pKa = 3.28KK56 pKa = 11.46DD57 pKa = 3.97VVAINCNTYY66 pKa = 9.15GYY68 pKa = 9.49SYY70 pKa = 11.47SVIEE74 pKa = 4.77KK75 pKa = 9.83DD76 pKa = 4.95DD77 pKa = 5.1LIIPPP82 pKa = 4.32

Molecular weight: 9.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D7SWQ5|A0A1D7SWQ5_9CAUD Mtd_N domain-containing protein OS=Cyanophage S-RIM12 OX=1278402 GN=Sn070910_092 PE=4 SV=1
MM1 pKa = 7.23IGLRR5 pKa = 11.84VRR7 pKa = 11.84IRR9 pKa = 11.84PKK11 pKa = 10.61SRR13 pKa = 11.84DD14 pKa = 3.5AIEE17 pKa = 3.89KK18 pKa = 9.79FRR20 pKa = 11.84YY21 pKa = 9.33DD22 pKa = 3.34MKK24 pKa = 10.7RR25 pKa = 11.84NNVMYY30 pKa = 9.6VTDD33 pKa = 5.22RR34 pKa = 11.84KK35 pKa = 10.51RR36 pKa = 11.84LWHH39 pKa = 6.68LFNPDD44 pKa = 2.68SGINLWVHH52 pKa = 5.96PTDD55 pKa = 3.99DD56 pKa = 4.85PNWEE60 pKa = 4.26IIRR63 pKa = 4.21

Molecular weight: 7.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

212

0

212

55688

35

6376

262.7

29.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.079 ± 0.214

0.851 ± 0.092

6.527 ± 0.129

6.057 ± 0.285

4.279 ± 0.117

7.872 ± 0.329

1.332 ± 0.115

6.298 ± 0.209

5.613 ± 0.421

7.251 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.248

5.831 ± 0.166

3.793 ± 0.11

3.724 ± 0.094

3.96 ± 0.192

7.362 ± 0.258

7.824 ± 0.452

6.865 ± 0.218

1.156 ± 0.09

4.204 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski