Malacosoma neustria nuclear polyhedrosis virus (MnNPV)

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus; unclassified Alphabaculovirus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 131 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343RRK8|A0A343RRK8_NPVMN p33 OS=Malacosoma neustria nuclear polyhedrosis virus OX=38012 GN=ManeNPV_00071 PE=4 SV=1
MM1 pKa = 7.7EE2 pKa = 5.54NNNNTDD8 pKa = 2.55RR9 pKa = 11.84HH10 pKa = 5.28EE11 pKa = 5.13DD12 pKa = 3.72YY13 pKa = 11.37NNSNMDD19 pKa = 3.36HH20 pKa = 6.78SDD22 pKa = 2.71YY23 pKa = 10.5WYY25 pKa = 10.22YY26 pKa = 10.47IPIEE30 pKa = 4.08NEE32 pKa = 3.7YY33 pKa = 9.18NTDD36 pKa = 3.38NNEE39 pKa = 4.86DD40 pKa = 3.2IDD42 pKa = 5.0YY43 pKa = 11.12DD44 pKa = 4.82NEE46 pKa = 4.75DD47 pKa = 3.63NDD49 pKa = 5.08DD50 pKa = 4.7DD51 pKa = 5.7EE52 pKa = 6.16EE53 pKa = 6.13FDD55 pKa = 6.29DD56 pKa = 6.67DD57 pKa = 6.58DD58 pKa = 7.52DD59 pKa = 7.49DD60 pKa = 7.53DD61 pKa = 7.35GDD63 pKa = 5.65DD64 pKa = 5.37DD65 pKa = 6.19DD66 pKa = 6.62NEE68 pKa = 4.34FNEE71 pKa = 4.3NNNNINIIIGSNVSISDD88 pKa = 3.26QSNINIVNIVVNTLLGINVVEE109 pKa = 4.35SMNFITNKK117 pKa = 6.01MHH119 pKa = 7.94RR120 pKa = 11.84IDD122 pKa = 3.57IQKK125 pKa = 11.13DD126 pKa = 3.14NDD128 pKa = 3.33NDD130 pKa = 3.79NFVLNGDD137 pKa = 3.99TCSVCLDD144 pKa = 3.9EE145 pKa = 4.71YY146 pKa = 11.1NGNIMMLKK154 pKa = 7.71EE155 pKa = 4.28CKK157 pKa = 10.15HH158 pKa = 5.95IFHH161 pKa = 7.07EE162 pKa = 4.75NCAKK166 pKa = 10.54AWFRR170 pKa = 11.84DD171 pKa = 3.43QRR173 pKa = 11.84TCPLCRR179 pKa = 11.84TVCYY183 pKa = 9.38HH184 pKa = 6.96ASIIKK189 pKa = 9.21TDD191 pKa = 3.45KK192 pKa = 11.39NEE194 pKa = 4.17CNKK197 pKa = 10.03NANEE201 pKa = 4.2LVNGTKK207 pKa = 10.31

Molecular weight: 24.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343RRE4|A0A343RRE4_NPVMN Uncharacterized protein OS=Malacosoma neustria nuclear polyhedrosis virus OX=38012 GN=ManeNPV_00006 PE=4 SV=1
MM1 pKa = 7.26LRR3 pKa = 11.84NRR5 pKa = 11.84IKK7 pKa = 10.79LKK9 pKa = 10.69RR10 pKa = 11.84RR11 pKa = 11.84MLLVKK16 pKa = 9.64KK17 pKa = 8.4TMSMMKK23 pKa = 9.67IVKK26 pKa = 9.9LLLTLPLPTLVMKK39 pKa = 10.9LPLILLLTFTQMKK52 pKa = 9.11MLLKK56 pKa = 9.76RR57 pKa = 11.84TTLMTRR63 pKa = 11.84ITSS66 pKa = 3.43

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

131

0

131

36384

52

1165

277.7

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.392 ± 0.192

2.221 ± 0.162

6.555 ± 0.163

5.571 ± 0.159

4.903 ± 0.164

3.26 ± 0.133

2.262 ± 0.091

7.589 ± 0.154

7.399 ± 0.273

9.114 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.817 ± 0.125

8.603 ± 0.186

3.194 ± 0.157

3.282 ± 0.189

4.461 ± 0.129

6.662 ± 0.179

5.621 ± 0.164

6.459 ± 0.189

0.723 ± 0.055

4.903 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski