Acinetobacter phage VB_ApiP_XC38
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8PR49|A0A5P8PR49_9CAUD Uncharacterized protein OS=Acinetobacter phage VB_ApiP_XC38 OX=2655002 GN=VBApiPXC38_62 PE=4 SV=1
MM1 pKa = 7.34 SLFVAWAAKK10 pKa = 8.45 VTVINYY16 pKa = 9.57 LGIAISVPTEE26 pKa = 3.69 FLEE29 pKa = 4.25 NGYY32 pKa = 9.78 IAMDD36 pKa = 3.23 VDD38 pKa = 4.09 GTVV41 pKa = 2.7
Molecular weight: 4.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 4.113
IPC_protein 3.694
Toseland 3.579
ProMoST 3.948
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.528
Solomon 3.63
Lehninger 3.592
Nozaki 3.935
DTASelect 3.986
Thurlkill 3.706
EMBOSS 3.732
Sillero 3.846
Patrickios 1.901
IPC_peptide 3.63
IPC2_peptide 3.795
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A5P8PR51|A0A5P8PR51_9CAUD Uncharacterized protein OS=Acinetobacter phage VB_ApiP_XC38 OX=2655002 GN=VBApiPXC38_49 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 9.69 RR3 pKa = 11.84 DD4 pKa = 3.44 QEE6 pKa = 4.41 IISFMVAILMSCVTAYY22 pKa = 10.77 VSIVRR27 pKa = 11.84 KK28 pKa = 9.47 VMRR31 pKa = 11.84 KK32 pKa = 9.04 RR33 pKa = 11.84 GKK35 pKa = 8.18 ITKK38 pKa = 9.0 LWLSSEE44 pKa = 4.16 VMMCLLAFLIALEE57 pKa = 4.92 LYY59 pKa = 9.46 PHH61 pKa = 7.28 LASLMPAFMTKK72 pKa = 10.29 AIFASTCVHH81 pKa = 5.64 MSSRR85 pKa = 11.84 LVMFLEE91 pKa = 4.16 EE92 pKa = 4.02 RR93 pKa = 11.84 VNRR96 pKa = 11.84 ALSSSS101 pKa = 3.42
Molecular weight: 11.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 9.004
IPC_protein 9.063
Toseland 9.604
ProMoST 9.399
Dawson 9.867
Bjellqvist 9.663
Wikipedia 10.043
Rodwell 10.116
Grimsley 9.926
Solomon 9.955
Lehninger 9.926
Nozaki 9.823
DTASelect 9.589
Thurlkill 9.721
EMBOSS 10.028
Sillero 9.853
Patrickios 7.571
IPC_peptide 9.94
IPC2_peptide 8.712
IPC2.peptide.svr19 7.853
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
96
0
96
24159
30
3323
251.7
28.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.75 ± 0.453
0.811 ± 0.162
5.977 ± 0.152
6.391 ± 0.263
3.427 ± 0.214
5.932 ± 0.179
1.838 ± 0.195
6.027 ± 0.241
6.726 ± 0.266
8.763 ± 0.218
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.736 ± 0.196
5.691 ± 0.233
3.564 ± 0.293
4.607 ± 0.288
4.143 ± 0.169
6.465 ± 0.494
6.817 ± 0.485
6.759 ± 0.214
1.018 ± 0.094
3.556 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here