Escherichia virus Lambda_2B8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambdavirus; unclassified Lambdavirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FQV5|A0A653FQV5_9CAUD Lom outer membrane protein OS=Escherichia virus Lambda_2B8 OX=2686050 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.4HH3 pKa = 5.21TEE5 pKa = 3.58LRR7 pKa = 11.84AAVLDD12 pKa = 3.88ALEE15 pKa = 4.33KK16 pKa = 10.45HH17 pKa = 6.2DD18 pKa = 4.25TGATLFDD25 pKa = 3.55GRR27 pKa = 11.84PAVFDD32 pKa = 3.71EE33 pKa = 4.56ADD35 pKa = 4.13FPAVAVYY42 pKa = 8.14LTGAEE47 pKa = 4.22YY48 pKa = 9.85TGEE51 pKa = 4.09EE52 pKa = 4.61LDD54 pKa = 3.6SDD56 pKa = 3.79TWQAEE61 pKa = 3.69LHH63 pKa = 6.35IEE65 pKa = 4.19VFLPAQVPDD74 pKa = 4.02SEE76 pKa = 4.66LDD78 pKa = 2.81AWMEE82 pKa = 3.93SRR84 pKa = 11.84IYY86 pKa = 10.51PVMSDD91 pKa = 3.25IPALSDD97 pKa = 5.02LITSMVASGYY107 pKa = 10.36DD108 pKa = 3.32YY109 pKa = 11.21RR110 pKa = 11.84RR111 pKa = 11.84DD112 pKa = 3.5DD113 pKa = 5.7DD114 pKa = 4.64AGLWSSADD122 pKa = 3.34LTYY125 pKa = 11.13VITYY129 pKa = 9.4EE130 pKa = 3.98MM131 pKa = 4.92

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FRB6|A0A653FRB6_9CAUD Uncharacterized protein OS=Escherichia virus Lambda_2B8 OX=2686050 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.6LILATRR8 pKa = 11.84NYY10 pKa = 8.65YY11 pKa = 10.51LEE13 pKa = 4.34YY14 pKa = 10.82GLRR17 pKa = 11.84LLLKK21 pKa = 8.56EE22 pKa = 4.37CRR24 pKa = 11.84VILAQEE30 pKa = 4.08FFMPEE35 pKa = 3.53NRR37 pKa = 11.84RR38 pKa = 11.84VILDD42 pKa = 3.76SKK44 pKa = 10.32EE45 pKa = 3.66SWLIICDD52 pKa = 3.71SQLGHH57 pKa = 7.29LMRR60 pKa = 11.84SMFQGRR66 pKa = 11.84RR67 pKa = 11.84FIQLDD72 pKa = 3.85LEE74 pKa = 4.32ALKK77 pKa = 10.84GGHH80 pKa = 7.29DD81 pKa = 3.15IHH83 pKa = 6.78NAVRR87 pKa = 11.84NRR89 pKa = 11.84LWTWNKK95 pKa = 9.52KK96 pKa = 9.25ARR98 pKa = 11.84ALTMSEE104 pKa = 3.98MVVMFGYY111 pKa = 10.05IYY113 pKa = 9.84RR114 pKa = 11.84QLRR117 pKa = 11.84PSHH120 pKa = 6.49LASEE124 pKa = 4.2MRR126 pKa = 11.84VNIKK130 pKa = 8.74TVNTFLYY137 pKa = 10.67SGLAKK142 pKa = 10.66NGLKK146 pKa = 10.26RR147 pKa = 11.84SSVRR151 pKa = 11.84LLAISEE157 pKa = 4.15NKK159 pKa = 10.31RR160 pKa = 11.84IGHH163 pKa = 5.66YY164 pKa = 10.47SGAGQGNHH172 pKa = 6.78AGGDD176 pKa = 3.8TGCGG180 pKa = 3.25

Molecular weight: 20.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

13503

38

1137

204.6

22.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.62 ± 0.561

1.289 ± 0.152

5.851 ± 0.209

6.48 ± 0.306

3.407 ± 0.221

7.613 ± 0.37

1.8 ± 0.191

5.103 ± 0.299

5.354 ± 0.285

7.739 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.888 ± 0.203

4.125 ± 0.227

4.058 ± 0.222

4.429 ± 0.268

6.221 ± 0.269

6.68 ± 0.232

6.206 ± 0.368

6.391 ± 0.361

1.696 ± 0.121

3.051 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski