Sinorhizobium phage PBC5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 7.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8W6G7|Q8W6G7_9CAUD Uncharacterized protein orf60 OS=Sinorhizobium phage PBC5 OX=179237 GN=orf60 PE=4 SV=1
MM1 pKa = 7.96AMRR4 pKa = 11.84RR5 pKa = 11.84WSCINCGATEE15 pKa = 4.32RR16 pKa = 11.84LTFYY20 pKa = 10.38PDD22 pKa = 3.41CCSSCGSAMVDD33 pKa = 3.33DD34 pKa = 5.22EE35 pKa = 4.62GRR37 pKa = 11.84STLAVEE43 pKa = 4.39PEE45 pKa = 4.4VPSEE49 pKa = 4.25LYY51 pKa = 10.44HH52 pKa = 6.75AAHH55 pKa = 7.03DD56 pKa = 3.94GDD58 pKa = 3.45RR59 pKa = 11.84AAIVEE64 pKa = 4.37LWHH67 pKa = 7.09AGALDD72 pKa = 3.74SSVGALRR79 pKa = 11.84GLLDD83 pKa = 5.78DD84 pKa = 4.41MLLEE88 pKa = 4.09NRR90 pKa = 11.84IDD92 pKa = 3.3MMMQVFSAPGRR103 pKa = 11.84EE104 pKa = 4.13AAA106 pKa = 4.83

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8W6F1|Q8W6F1_9CAUD Uncharacterized protein orf76 OS=Sinorhizobium phage PBC5 OX=179237 GN=orf76 PE=4 SV=1
MM1 pKa = 7.11NVSRR5 pKa = 11.84PAGSCPAWKK14 pKa = 9.28KK15 pKa = 8.87WRR17 pKa = 11.84MEE19 pKa = 3.82ASSAKK24 pKa = 9.43SACVMRR30 pKa = 11.84RR31 pKa = 11.84STRR34 pKa = 11.84RR35 pKa = 11.84HH36 pKa = 5.37CSRR39 pKa = 11.84RR40 pKa = 11.84ASALNFGFRR49 pKa = 11.84IRR51 pKa = 11.84RR52 pKa = 11.84SNIRR56 pKa = 11.84CRR58 pKa = 11.84VLIAFQRR65 pKa = 11.84SLSRR69 pKa = 11.84CGSLFQQPRR78 pKa = 11.84QMMYY82 pKa = 10.58LEE84 pKa = 4.85SGNFSRR90 pKa = 11.84GAVRR94 pKa = 11.84RR95 pKa = 11.84HH96 pKa = 5.19VGLMQAMRR104 pKa = 11.84SVIGHH109 pKa = 6.19HH110 pKa = 5.12RR111 pKa = 11.84HH112 pKa = 5.77GVGEE116 pKa = 4.41SPSGLCQPDD125 pKa = 3.41VAVAGNFDD133 pKa = 3.66EE134 pKa = 4.38AHH136 pKa = 6.25AVHH139 pKa = 6.1VHH141 pKa = 6.26ADD143 pKa = 3.56GVVIALVEE151 pKa = 4.06NTLLRR156 pKa = 11.84RR157 pKa = 11.84RR158 pKa = 11.84GSPAVRR164 pKa = 11.84LACPARR170 pKa = 11.84HH171 pKa = 6.31LFRR174 pKa = 11.84FRR176 pKa = 11.84AAYY179 pKa = 10.29LSGAKK184 pKa = 9.59QCDD187 pKa = 3.45LCIDD191 pKa = 3.69QVLDD195 pKa = 3.62EE196 pKa = 4.0QLFRR200 pKa = 11.84HH201 pKa = 5.1GQEE204 pKa = 3.59RR205 pKa = 11.84ALNLSLAGADD215 pKa = 3.29IRR217 pKa = 11.84EE218 pKa = 4.29HH219 pKa = 6.12PGLRR223 pKa = 11.84GRR225 pKa = 11.84NLEE228 pKa = 4.12GCGNLPAGLTANGAAVCDD246 pKa = 3.89ASEE249 pKa = 4.52LEE251 pKa = 4.12IHH253 pKa = 6.44HH254 pKa = 6.85LCHH257 pKa = 6.59AATFGKK263 pKa = 10.39SLL265 pKa = 3.62

Molecular weight: 29.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

25661

80

2849

309.2

33.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.773 ± 0.298

1.418 ± 0.246

6.056 ± 0.23

5.881 ± 0.327

3.032 ± 0.155

8.55 ± 0.312

2.533 ± 0.215

4.528 ± 0.178

3.8 ± 0.325

8.663 ± 0.391

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.131

2.818 ± 0.204

5.039 ± 0.262

3.507 ± 0.18

9.302 ± 0.483

6.099 ± 0.33

5.019 ± 0.266

6.827 ± 0.3

1.376 ± 0.11

1.648 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski