Hepatitis B virus subtype adw2
Average proteome isoelectric point is 8.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q17UT4|Q17UT4_HBVAW Capsid protein OS=Hepatitis B virus subtype adw2 OX=10408 GN=pre C/C PE=3 SV=1
MM1 pKa = 7.1 SHH3 pKa = 6.99 CSSLQAVPWVALGHH17 pKa = 5.4 GHH19 pKa = 7.28
Molecular weight: 2.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.578
IPC2_protein 6.287
IPC_protein 6.854
Toseland 6.956
ProMoST 6.389
Dawson 7.22
Bjellqvist 6.795
Wikipedia 7.205
Rodwell 7.161
Grimsley 7.029
Solomon 7.395
Lehninger 7.41
Nozaki 7.19
DTASelect 7.454
Thurlkill 7.483
EMBOSS 7.614
Sillero 7.527
Patrickios 2.409
IPC_peptide 7.395
IPC2_peptide 7.468
IPC2.peptide.svr19 7.527
Protein with the highest isoelectric point:
>tr|Q17UT5|Q17UT5_HBVAW Protein X OS=Hepatitis B virus subtype adw2 OX=10408 GN=X PE=3 SV=1
MM1 pKa = 7.31 QLFHH5 pKa = 7.21 LCLIISCTCPTVQASKK21 pKa = 11.2 LCLGWLWGMDD31 pKa = 2.87 IDD33 pKa = 4.46 PYY35 pKa = 11.25 KK36 pKa = 10.85 EE37 pKa = 4.0 FGATVEE43 pKa = 4.56 LLSFLPSDD51 pKa = 4.16 FFPSVRR57 pKa = 11.84 DD58 pKa = 3.78 LLDD61 pKa = 3.57 TASALYY67 pKa = 10.4 RR68 pKa = 11.84 EE69 pKa = 4.55 ALEE72 pKa = 4.62 SPEE75 pKa = 4.18 HH76 pKa = 6.65 CSPHH80 pKa = 5.29 HH81 pKa = 5.26 TALRR85 pKa = 11.84 QAILCWGEE93 pKa = 4.32 LMTLATWVGNNLEE106 pKa = 4.75 DD107 pKa = 3.84 PASRR111 pKa = 11.84 DD112 pKa = 3.34 LVVNYY117 pKa = 10.33 VNTNMGLKK125 pKa = 9.51 IRR127 pKa = 11.84 QLLWFHH133 pKa = 6.76 ISCLTFGRR141 pKa = 11.84 EE142 pKa = 3.77 TVLEE146 pKa = 4.1 YY147 pKa = 10.79 LVSFGVWIRR156 pKa = 11.84 TPPAYY161 pKa = 9.91 RR162 pKa = 11.84 PPNAPILSTLPEE174 pKa = 4.13 TTVVRR179 pKa = 11.84 RR180 pKa = 11.84 RR181 pKa = 11.84 DD182 pKa = 3.21 RR183 pKa = 11.84 GRR185 pKa = 11.84 SPRR188 pKa = 11.84 RR189 pKa = 11.84 RR190 pKa = 11.84 TPSPRR195 pKa = 11.84 RR196 pKa = 11.84 RR197 pKa = 11.84 RR198 pKa = 11.84 SQSPRR203 pKa = 11.84 RR204 pKa = 11.84 RR205 pKa = 11.84 RR206 pKa = 11.84 SQSRR210 pKa = 11.84 EE211 pKa = 4.05 SQCC214 pKa = 4.24
Molecular weight: 24.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.939
IPC2_protein 8.639
IPC_protein 8.785
Toseland 8.712
ProMoST 9.063
Dawson 9.268
Bjellqvist 9.487
Wikipedia 9.355
Rodwell 9.282
Grimsley 8.756
Solomon 9.428
Lehninger 9.414
Nozaki 9.663
DTASelect 9.253
Thurlkill 9.282
EMBOSS 9.414
Sillero 9.56
Patrickios 4.8
IPC_peptide 9.414
IPC2_peptide 9.165
IPC2.peptide.svr19 8.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2324
19
845
290.5
32.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.508 ± 0.581
3.657 ± 0.606
2.797 ± 0.31
2.453 ± 0.728
5.12 ± 0.43
6.54 ± 0.555
2.797 ± 0.673
4.561 ± 0.615
2.453 ± 0.565
11.833 ± 0.515
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.893 ± 0.258
3.485 ± 0.384
9.036 ± 0.756
3.744 ± 0.401
6.11 ± 1.419
10.327 ± 0.354
6.153 ± 0.538
5.637 ± 0.283
3.184 ± 0.63
2.711 ± 0.401
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here