Dickeya phage Ninurta
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S1GTF1|A0A2S1GTF1_9CAUD DNA-directed DNA polymerase OS=Dickeya phage Ninurta OX=2163631 PE=3 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 SYY4 pKa = 9.3 EE5 pKa = 4.31 TTLEE9 pKa = 4.07 TSEE12 pKa = 4.12 EE13 pKa = 4.23 LEE15 pKa = 4.29 GVNEE19 pKa = 4.02 VLASIGEE26 pKa = 4.37 PPVSTLEE33 pKa = 3.98 GDD35 pKa = 3.8 ANADD39 pKa = 3.18 VANARR44 pKa = 11.84 RR45 pKa = 11.84 ILNTVNRR52 pKa = 11.84 QIQSKK57 pKa = 8.47 GWTYY61 pKa = 10.96 NIEE64 pKa = 4.16 EE65 pKa = 5.0 LDD67 pKa = 3.83 LVPDD71 pKa = 4.27 VFSKK75 pKa = 10.76 LIPYY79 pKa = 8.98 MSDD82 pKa = 2.97 YY83 pKa = 11.22 LRR85 pKa = 11.84 VFNDD89 pKa = 3.18 SGATQYY95 pKa = 11.07 INRR98 pKa = 11.84 GGYY101 pKa = 10.54 LYY103 pKa = 9.22 DD104 pKa = 3.61 TTNKK108 pKa = 9.03 TDD110 pKa = 3.08 QFTSSVTVSIIRR122 pKa = 11.84 LKK124 pKa = 10.56 EE125 pKa = 3.8 FNEE128 pKa = 3.91 MPEE131 pKa = 4.26 CFRR134 pKa = 11.84 SYY136 pKa = 10.67 IIAKK140 pKa = 10.26 ASRR143 pKa = 11.84 QFNMQFFGATEE154 pKa = 3.7 IDD156 pKa = 3.34 AFLQEE161 pKa = 5.1 RR162 pKa = 11.84 EE163 pKa = 4.29 LEE165 pKa = 4.22 CWRR168 pKa = 11.84 DD169 pKa = 3.48 VQTYY173 pKa = 9.61 EE174 pKa = 4.93 LDD176 pKa = 3.51 YY177 pKa = 11.61 GNFNMLDD184 pKa = 3.4 GDD186 pKa = 4.36 SFVGGLLSRR195 pKa = 4.64
Molecular weight: 22.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.369
IPC2_protein 4.355
IPC_protein 4.279
Toseland 4.113
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.101
Rodwell 4.113
Grimsley 4.024
Solomon 4.228
Lehninger 4.177
Nozaki 4.329
DTASelect 4.482
Thurlkill 4.126
EMBOSS 4.113
Sillero 4.393
Patrickios 3.567
IPC_peptide 4.228
IPC2_peptide 4.38
IPC2.peptide.svr19 4.308
Protein with the highest isoelectric point:
>tr|A0A2S1GTA5|A0A2S1GTA5_9CAUD dGTP triphosphohydrolase inhibitor OS=Dickeya phage Ninurta OX=2163631 PE=4 SV=1
MM1 pKa = 7.74 AKK3 pKa = 9.32 PHH5 pKa = 5.99 NFRR8 pKa = 11.84 VVHH11 pKa = 6.53 RR12 pKa = 11.84 YY13 pKa = 9.18 GVTTTPRR20 pKa = 11.84 VIQDD24 pKa = 3.67 LNTGRR29 pKa = 11.84 LHH31 pKa = 6.95 PLKK34 pKa = 10.48 QQTPHH39 pKa = 6.31 IAKK42 pKa = 10.39 AGGLTFHH49 pKa = 7.29 ILKK52 pKa = 10.4 AHH54 pKa = 6.66 TGTSNHH60 pKa = 5.3 VVVKK64 pKa = 9.51 PYY66 pKa = 10.34 EE67 pKa = 4.54 GNWPRR72 pKa = 11.84 LSYY75 pKa = 9.74 YY76 pKa = 9.79 VKK78 pKa = 10.61 RR79 pKa = 11.84 GLEE82 pKa = 3.93 LFAA85 pKa = 6.87
Molecular weight: 9.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 10.101
IPC_protein 10.73
Toseland 10.745
ProMoST 10.409
Dawson 10.862
Bjellqvist 10.555
Wikipedia 11.052
Rodwell 11.242
Grimsley 10.921
Solomon 10.935
Lehninger 10.906
Nozaki 10.701
DTASelect 10.555
Thurlkill 10.745
EMBOSS 11.125
Sillero 10.774
Patrickios 10.994
IPC_peptide 10.935
IPC2_peptide 9.326
IPC2.peptide.svr19 8.25
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
12044
43
1295
261.8
29.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.158 ± 0.483
0.805 ± 0.141
6.501 ± 0.272
6.551 ± 0.299
3.736 ± 0.23
7.564 ± 0.257
1.876 ± 0.199
5.007 ± 0.19
6.543 ± 0.322
7.888 ± 0.303
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.856 ± 0.166
4.758 ± 0.274
3.496 ± 0.215
4.401 ± 0.436
5.289 ± 0.2
5.961 ± 0.277
5.903 ± 0.268
6.908 ± 0.315
1.453 ± 0.177
3.346 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here