Campylobacter phage CP20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Eucampyvirinae; Firehammervirus; unclassified Firehammervirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A410T7E2|A0A410T7E2_9CAUD Uncharacterized protein OS=Campylobacter phage CP20 OX=2506428 PE=4 SV=1
MM1 pKa = 6.82YY2 pKa = 9.99VARR5 pKa = 11.84FNEE8 pKa = 4.17YY9 pKa = 10.17FGYY12 pKa = 10.27DD13 pKa = 3.69YY14 pKa = 11.5QLMDD18 pKa = 3.94VEE20 pKa = 4.74TVDD23 pKa = 3.5ILEE26 pKa = 4.93NIYY29 pKa = 11.06DD30 pKa = 3.83SLGYY34 pKa = 9.2MPRR37 pKa = 11.84DD38 pKa = 3.63PEE40 pKa = 5.28PIDD43 pKa = 3.86LEE45 pKa = 4.12IVLEE49 pKa = 4.09EE50 pKa = 4.85FYY52 pKa = 10.45NTDD55 pKa = 3.36KK56 pKa = 11.01IYY58 pKa = 10.82VLCEE62 pKa = 3.82ADD64 pKa = 3.57DD65 pKa = 4.27EE66 pKa = 4.81EE67 pKa = 6.57IEE69 pKa = 4.29FCSCIDD75 pKa = 3.43EE76 pKa = 4.4VAPEE80 pKa = 4.02VIIDD84 pKa = 3.62FLKK87 pKa = 11.06SKK89 pKa = 10.08GHH91 pKa = 5.6TFIDD95 pKa = 4.05SNAA98 pKa = 3.35

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A410T7M6|A0A410T7M6_9CAUD Uncharacterized protein OS=Campylobacter phage CP20 OX=2506428 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 4.46SLKK5 pKa = 10.73LVKK8 pKa = 10.48NLIKK12 pKa = 10.02TKK14 pKa = 10.3SVEE17 pKa = 3.74KK18 pKa = 10.81GQILKK23 pKa = 10.18PGNLVIFKK31 pKa = 9.51YY32 pKa = 10.38NPKK35 pKa = 8.84DD36 pKa = 3.31TSVKK40 pKa = 9.92YY41 pKa = 10.65DD42 pKa = 3.6RR43 pKa = 11.84TPLCLVLRR51 pKa = 11.84KK52 pKa = 9.82SKK54 pKa = 10.75SYY56 pKa = 8.62TLGINFHH63 pKa = 6.52WCPIPMRR70 pKa = 11.84KK71 pKa = 8.28MLLNAIFRR79 pKa = 11.84LNKK82 pKa = 10.16KK83 pKa = 9.73NIKK86 pKa = 8.7EE87 pKa = 4.02NKK89 pKa = 9.04PLDD92 pKa = 3.8IDD94 pKa = 3.93WYY96 pKa = 10.34RR97 pKa = 11.84IKK99 pKa = 10.97PMLKK103 pKa = 9.73KK104 pKa = 10.66FGFFPIIRR112 pKa = 11.84LYY114 pKa = 10.15INSRR118 pKa = 11.84IYY120 pKa = 10.45RR121 pKa = 11.84RR122 pKa = 11.84AVKK125 pKa = 10.12IPNEE129 pKa = 3.81NMKK132 pKa = 10.57QIIEE136 pKa = 4.46TKK138 pKa = 8.35TEE140 pKa = 3.87TFIGVSAEE148 pKa = 3.86ALYY151 pKa = 10.78KK152 pKa = 10.45KK153 pKa = 10.29ALRR156 pKa = 11.84DD157 pKa = 3.61SKK159 pKa = 11.38VSSKK163 pKa = 10.79SKK165 pKa = 10.43KK166 pKa = 9.75

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

202

0

202

52010

37

1386

257.5

29.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.426 ± 0.156

1.473 ± 0.105

6.868 ± 0.124

6.503 ± 0.175

5.216 ± 0.138

4.559 ± 0.124

1.342 ± 0.062

8.7 ± 0.154

9.669 ± 0.18

8.808 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.757 ± 0.063

8.123 ± 0.154

2.838 ± 0.074

2.898 ± 0.114

3.028 ± 0.086

6.951 ± 0.189

5.626 ± 0.145

5.312 ± 0.131

0.686 ± 0.043

5.216 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski