Staphylococcus virus 3a

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q4ZCN9|Q4ZCN9_9CAUD ORF047 OS=Staphylococcus virus 3a OX=215167 PE=4 SV=1
MM1 pKa = 7.51NNRR4 pKa = 11.84EE5 pKa = 4.1QIEE8 pKa = 3.98QSVISASAYY17 pKa = 10.04NGNDD21 pKa = 3.29TEE23 pKa = 4.52GLLKK27 pKa = 10.49EE28 pKa = 4.44IEE30 pKa = 4.24DD31 pKa = 4.28VYY33 pKa = 11.57KK34 pKa = 10.64KK35 pKa = 10.72AQAFDD40 pKa = 4.66EE41 pKa = 4.34ILEE44 pKa = 4.2GLPNAMQDD52 pKa = 4.46AIKK55 pKa = 10.61EE56 pKa = 4.15DD57 pKa = 3.37IGLDD61 pKa = 3.5EE62 pKa = 5.32AVGIMTGQVVYY73 pKa = 10.25KK74 pKa = 10.67YY75 pKa = 10.68EE76 pKa = 3.97EE77 pKa = 4.06EE78 pKa = 4.28QEE80 pKa = 3.93NDD82 pKa = 2.92

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4ZCT7|Q4ZCT7_9CAUD ORF046 OS=Staphylococcus virus 3a OX=215167 PE=4 SV=1
MM1 pKa = 7.71TKK3 pKa = 8.07MTWNTRR9 pKa = 11.84FFVQRR14 pKa = 11.84LDD16 pKa = 3.36ISLQEE21 pKa = 3.73IMKK24 pKa = 10.31VVGTQMTNLEE34 pKa = 4.15ARR36 pKa = 11.84FLMKK40 pKa = 9.98RR41 pKa = 11.84DD42 pKa = 3.68CKK44 pKa = 10.68KK45 pKa = 10.86

Molecular weight: 5.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

13819

34

1649

206.3

23.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.963 ± 0.542

0.543 ± 0.1

6.303 ± 0.212

7.569 ± 0.346

4.11 ± 0.238

6.071 ± 0.41

1.751 ± 0.151

7.099 ± 0.306

9.653 ± 0.587

8.597 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.125

6.194 ± 0.266

2.779 ± 0.239

3.553 ± 0.144

3.958 ± 0.187

6.578 ± 0.33

5.615 ± 0.217

5.572 ± 0.22

1.274 ± 0.11

4.298 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski