Amino acid dipepetide frequency for Mycobacterium phage PBI1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.215AlaAla: 10.215 ± 0.753
0.648AlaCys: 0.648 ± 0.166
6.079AlaAsp: 6.079 ± 0.575
7.823AlaGlu: 7.823 ± 0.88
2.193AlaPhe: 2.193 ± 0.284
7.126AlaGly: 7.126 ± 0.697
1.545AlaHis: 1.545 ± 0.266
4.236AlaIle: 4.236 ± 0.536
5.531AlaLys: 5.531 ± 0.731
8.172AlaLeu: 8.172 ± 0.78
2.342AlaMet: 2.342 ± 0.311
3.588AlaAsn: 3.588 ± 0.444
3.986AlaPro: 3.986 ± 0.355
3.737AlaGln: 3.737 ± 0.47
4.784AlaArg: 4.784 ± 0.521
5.332AlaSer: 5.332 ± 0.483
4.834AlaThr: 4.834 ± 0.57
5.88AlaVal: 5.88 ± 0.66
1.495AlaTrp: 1.495 ± 0.239
2.94AlaTyr: 2.94 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
1.046CysAla: 1.046 ± 0.215
0.199CysCys: 0.199 ± 0.084
0.548CysAsp: 0.548 ± 0.208
0.698CysGlu: 0.698 ± 0.214
0.349CysPhe: 0.349 ± 0.127
0.648CysGly: 0.648 ± 0.223
0.299CysHis: 0.299 ± 0.124
0.399CysIle: 0.399 ± 0.142
0.349CysLys: 0.349 ± 0.126
0.648CysLeu: 0.648 ± 0.221
0.199CysMet: 0.199 ± 0.117
0.498CysAsn: 0.498 ± 0.173
0.947CysPro: 0.947 ± 0.326
0.249CysGln: 0.249 ± 0.097
0.498CysArg: 0.498 ± 0.168
0.648CysSer: 0.648 ± 0.176
0.498CysThr: 0.498 ± 0.15
0.448CysVal: 0.448 ± 0.159
0.1CysTrp: 0.1 ± 0.072
0.399CysTyr: 0.399 ± 0.12
0.0CysXaa: 0.0 ± 0.0
Asp
5.731AspAla: 5.731 ± 0.45
0.349AspCys: 0.349 ± 0.142
6.677AspAsp: 6.677 ± 1.394
5.78AspGlu: 5.78 ± 1.204
2.292AspPhe: 2.292 ± 0.322
5.531AspGly: 5.531 ± 0.55
1.296AspHis: 1.296 ± 0.28
2.94AspIle: 2.94 ± 0.42
3.239AspLys: 3.239 ± 0.478
6.229AspLeu: 6.229 ± 0.656
1.345AspMet: 1.345 ± 0.254
2.193AspAsn: 2.193 ± 0.316
4.086AspPro: 4.086 ± 0.394
1.943AspGln: 1.943 ± 0.312
4.883AspArg: 4.883 ± 0.5
3.438AspSer: 3.438 ± 0.517
3.289AspThr: 3.289 ± 0.466
4.236AspVal: 4.236 ± 0.422
1.445AspTrp: 1.445 ± 0.341
2.292AspTyr: 2.292 ± 0.264
0.0AspXaa: 0.0 ± 0.0
Glu
6.229GluAla: 6.229 ± 0.643
0.548GluCys: 0.548 ± 0.224
6.328GluAsp: 6.328 ± 1.275
6.029GluGlu: 6.029 ± 0.618
2.741GluPhe: 2.741 ± 0.446
4.236GluGly: 4.236 ± 0.451
1.395GluHis: 1.395 ± 0.277
3.538GluIle: 3.538 ± 0.348
2.442GluLys: 2.442 ± 0.462
6.827GluLeu: 6.827 ± 0.571
1.694GluMet: 1.694 ± 0.288
2.093GluAsn: 2.093 ± 0.276
2.292GluPro: 2.292 ± 0.383
2.89GluGln: 2.89 ± 0.477
5.382GluArg: 5.382 ± 0.737
3.986GluSer: 3.986 ± 0.351
2.741GluThr: 2.741 ± 0.375
4.385GluVal: 4.385 ± 0.532
1.595GluTrp: 1.595 ± 0.288
1.794GluTyr: 1.794 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
2.442PheAla: 2.442 ± 0.385
0.399PheCys: 0.399 ± 0.148
2.292PheAsp: 2.292 ± 0.324
2.093PheGlu: 2.093 ± 0.337
0.797PhePhe: 0.797 ± 0.197
3.737PheGly: 3.737 ± 0.42
0.598PheHis: 0.598 ± 0.176
1.445PheIle: 1.445 ± 0.329
1.794PheLys: 1.794 ± 0.338
2.591PheLeu: 2.591 ± 0.502
0.548PheMet: 0.548 ± 0.182
1.445PheAsn: 1.445 ± 0.296
1.545PhePro: 1.545 ± 0.323
1.395PheGln: 1.395 ± 0.26
2.392PheArg: 2.392 ± 0.423
1.744PheSer: 1.744 ± 0.34
1.694PheThr: 1.694 ± 0.27
1.545PheVal: 1.545 ± 0.269
0.448PheTrp: 0.448 ± 0.159
1.146PheTyr: 1.146 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
6.478GlyAla: 6.478 ± 0.783
1.196GlyCys: 1.196 ± 0.246
5.083GlyAsp: 5.083 ± 0.506
5.033GlyGlu: 5.033 ± 0.477
2.541GlyPhe: 2.541 ± 0.36
5.731GlyGly: 5.731 ± 0.692
1.495GlyHis: 1.495 ± 0.265
4.435GlyIle: 4.435 ± 0.549
5.332GlyLys: 5.332 ± 0.445
6.279GlyLeu: 6.279 ± 0.695
2.342GlyMet: 2.342 ± 0.308
2.541GlyAsn: 2.541 ± 0.313
4.335GlyPro: 4.335 ± 0.494
2.84GlyGln: 2.84 ± 0.359
3.937GlyArg: 3.937 ± 0.397
5.98GlySer: 5.98 ± 0.712
6.627GlyThr: 6.627 ± 0.716
5.133GlyVal: 5.133 ± 0.535
1.595GlyTrp: 1.595 ± 0.298
3.189GlyTyr: 3.189 ± 0.41
0.0GlyXaa: 0.0 ± 0.0
His
1.644HisAla: 1.644 ± 0.327
0.249HisCys: 0.249 ± 0.095
1.096HisAsp: 1.096 ± 0.233
1.096HisGlu: 1.096 ± 0.268
0.947HisPhe: 0.947 ± 0.208
1.844HisGly: 1.844 ± 0.265
0.349HisHis: 0.349 ± 0.167
0.897HisIle: 0.897 ± 0.174
0.997HisLys: 0.997 ± 0.198
1.844HisLeu: 1.844 ± 0.385
0.498HisMet: 0.498 ± 0.15
0.997HisAsn: 0.997 ± 0.241
1.345HisPro: 1.345 ± 0.295
0.598HisGln: 0.598 ± 0.183
1.246HisArg: 1.246 ± 0.289
0.548HisSer: 0.548 ± 0.169
0.797HisThr: 0.797 ± 0.182
1.196HisVal: 1.196 ± 0.246
0.199HisTrp: 0.199 ± 0.122
0.747HisTyr: 0.747 ± 0.186
0.0HisXaa: 0.0 ± 0.0
Ile
4.535IleAla: 4.535 ± 0.63
0.498IleCys: 0.498 ± 0.144
3.239IleAsp: 3.239 ± 0.385
2.94IleGlu: 2.94 ± 0.425
1.345IlePhe: 1.345 ± 0.226
3.638IleGly: 3.638 ± 0.43
0.797IleHis: 0.797 ± 0.172
2.591IleIle: 2.591 ± 0.369
2.392IleLys: 2.392 ± 0.352
3.139IleLeu: 3.139 ± 0.363
1.196IleMet: 1.196 ± 0.25
2.791IleAsn: 2.791 ± 0.396
3.089IlePro: 3.089 ± 0.47
2.193IleGln: 2.193 ± 0.513
3.687IleArg: 3.687 ± 0.38
2.442IleSer: 2.442 ± 0.317
3.239IleThr: 3.239 ± 0.405
3.239IleVal: 3.239 ± 0.427
0.847IleTrp: 0.847 ± 0.211
1.096IleTyr: 1.096 ± 0.236
0.0IleXaa: 0.0 ± 0.0
Lys
5.98LysAla: 5.98 ± 0.844
0.448LysCys: 0.448 ± 0.142
3.538LysAsp: 3.538 ± 0.363
3.687LysGlu: 3.687 ± 0.537
1.445LysPhe: 1.445 ± 0.217
3.438LysGly: 3.438 ± 0.545
1.146LysHis: 1.146 ± 0.27
2.99LysIle: 2.99 ± 0.353
3.139LysLys: 3.139 ± 0.451
4.485LysLeu: 4.485 ± 0.47
0.997LysMet: 0.997 ± 0.179
2.043LysAsn: 2.043 ± 0.399
3.289LysPro: 3.289 ± 0.378
1.794LysGln: 1.794 ± 0.277
4.385LysArg: 4.385 ± 0.572
3.04LysSer: 3.04 ± 0.31
2.99LysThr: 2.99 ± 0.436
3.687LysVal: 3.687 ± 0.367
1.096LysTrp: 1.096 ± 0.215
1.296LysTyr: 1.296 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
7.425LeuAla: 7.425 ± 0.612
0.498LeuCys: 0.498 ± 0.197
5.033LeuAsp: 5.033 ± 0.577
5.033LeuGlu: 5.033 ± 0.565
2.442LeuPhe: 2.442 ± 0.368
6.079LeuGly: 6.079 ± 0.723
1.545LeuHis: 1.545 ± 0.292
2.94LeuIle: 2.94 ± 0.432
4.933LeuLys: 4.933 ± 0.529
5.731LeuLeu: 5.731 ± 0.504
1.794LeuMet: 1.794 ± 0.262
3.388LeuAsn: 3.388 ± 0.405
4.435LeuPro: 4.435 ± 0.513
2.541LeuGln: 2.541 ± 0.379
5.481LeuArg: 5.481 ± 0.6
5.182LeuSer: 5.182 ± 0.523
5.033LeuThr: 5.033 ± 0.498
4.285LeuVal: 4.285 ± 0.421
1.146LeuTrp: 1.146 ± 0.267
1.345LeuTyr: 1.345 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
2.492MetAla: 2.492 ± 0.277
0.199MetCys: 0.199 ± 0.094
1.196MetAsp: 1.196 ± 0.215
1.096MetGlu: 1.096 ± 0.203
0.648MetPhe: 0.648 ± 0.172
1.545MetGly: 1.545 ± 0.262
0.399MetHis: 0.399 ± 0.131
1.196MetIle: 1.196 ± 0.22
1.395MetLys: 1.395 ± 0.224
1.246MetLeu: 1.246 ± 0.339
0.548MetMet: 0.548 ± 0.19
0.997MetAsn: 0.997 ± 0.213
1.395MetPro: 1.395 ± 0.26
0.847MetGln: 0.847 ± 0.212
1.495MetArg: 1.495 ± 0.25
1.694MetSer: 1.694 ± 0.26
1.545MetThr: 1.545 ± 0.221
0.997MetVal: 0.997 ± 0.238
0.498MetTrp: 0.498 ± 0.167
1.046MetTyr: 1.046 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
4.036AsnAla: 4.036 ± 0.53
0.149AsnCys: 0.149 ± 0.098
2.641AsnAsp: 2.641 ± 0.43
2.442AsnGlu: 2.442 ± 0.372
1.345AsnPhe: 1.345 ± 0.326
3.687AsnGly: 3.687 ± 0.563
0.897AsnHis: 0.897 ± 0.176
1.894AsnIle: 1.894 ± 0.318
2.193AsnLys: 2.193 ± 0.353
2.292AsnLeu: 2.292 ± 0.312
0.747AsnMet: 0.747 ± 0.211
1.495AsnAsn: 1.495 ± 0.333
3.089AsnPro: 3.089 ± 0.51
1.345AsnGln: 1.345 ± 0.285
2.94AsnArg: 2.94 ± 0.38
2.093AsnSer: 2.093 ± 0.303
2.093AsnThr: 2.093 ± 0.274
2.093AsnVal: 2.093 ± 0.251
0.648AsnTrp: 0.648 ± 0.198
1.445AsnTyr: 1.445 ± 0.261
0.0AsnXaa: 0.0 ± 0.0
Pro
4.036ProAla: 4.036 ± 0.439
0.399ProCys: 0.399 ± 0.162
3.139ProAsp: 3.139 ± 0.379
4.186ProGlu: 4.186 ± 0.431
1.943ProPhe: 1.943 ± 0.327
5.182ProGly: 5.182 ± 0.623
1.246ProHis: 1.246 ± 0.177
2.242ProIle: 2.242 ± 0.274
3.289ProLys: 3.289 ± 0.49
3.438ProLeu: 3.438 ± 0.415
0.797ProMet: 0.797 ± 0.211
2.741ProAsn: 2.741 ± 0.404
2.342ProPro: 2.342 ± 0.413
1.694ProGln: 1.694 ± 0.317
2.193ProArg: 2.193 ± 0.288
3.588ProSer: 3.588 ± 0.367
3.189ProThr: 3.189 ± 0.329
3.139ProVal: 3.139 ± 0.317
1.146ProTrp: 1.146 ± 0.302
1.644ProTyr: 1.644 ± 0.292
0.0ProXaa: 0.0 ± 0.0
Gln
3.04GlnAla: 3.04 ± 0.469
0.199GlnCys: 0.199 ± 0.088
2.342GlnAsp: 2.342 ± 0.301
1.993GlnGlu: 1.993 ± 0.332
1.644GlnPhe: 1.644 ± 0.24
2.741GlnGly: 2.741 ± 0.413
0.598GlnHis: 0.598 ± 0.175
2.641GlnIle: 2.641 ± 0.287
2.143GlnLys: 2.143 ± 0.387
2.691GlnLeu: 2.691 ± 0.384
1.046GlnMet: 1.046 ± 0.198
1.844GlnAsn: 1.844 ± 0.269
1.345GlnPro: 1.345 ± 0.3
1.345GlnGln: 1.345 ± 0.299
2.741GlnArg: 2.741 ± 0.387
2.143GlnSer: 2.143 ± 0.349
1.794GlnThr: 1.794 ± 0.312
2.392GlnVal: 2.392 ± 0.35
0.498GlnTrp: 0.498 ± 0.152
0.847GlnTyr: 0.847 ± 0.176
0.0GlnXaa: 0.0 ± 0.0
Arg
5.731ArgAla: 5.731 ± 0.601
0.498ArgCys: 0.498 ± 0.176
4.036ArgAsp: 4.036 ± 0.54
4.285ArgGlu: 4.285 ± 0.569
1.894ArgPhe: 1.894 ± 0.27
4.983ArgGly: 4.983 ± 0.39
0.997ArgHis: 0.997 ± 0.244
3.488ArgIle: 3.488 ± 0.489
4.584ArgLys: 4.584 ± 0.644
4.186ArgLeu: 4.186 ± 0.458
1.894ArgMet: 1.894 ± 0.353
2.591ArgAsn: 2.591 ± 0.47
2.641ArgPro: 2.641 ± 0.394
2.641ArgGln: 2.641 ± 0.307
5.083ArgArg: 5.083 ± 0.629
3.638ArgSer: 3.638 ± 0.456
3.438ArgThr: 3.438 ± 0.397
5.282ArgVal: 5.282 ± 0.576
1.595ArgTrp: 1.595 ± 0.315
2.093ArgTyr: 2.093 ± 0.328
0.0ArgXaa: 0.0 ± 0.0
Ser
6.279SerAla: 6.279 ± 0.618
0.897SerCys: 0.897 ± 0.248
4.634SerAsp: 4.634 ± 0.561
3.139SerGlu: 3.139 ± 0.434
2.292SerPhe: 2.292 ± 0.39
6.627SerGly: 6.627 ± 0.587
0.897SerHis: 0.897 ± 0.194
2.492SerIle: 2.492 ± 0.463
2.442SerLys: 2.442 ± 0.341
3.986SerLeu: 3.986 ± 0.548
1.495SerMet: 1.495 ± 0.262
1.744SerAsn: 1.744 ± 0.29
2.442SerPro: 2.442 ± 0.344
2.193SerGln: 2.193 ± 0.357
3.289SerArg: 3.289 ± 0.398
4.036SerSer: 4.036 ± 0.506
4.335SerThr: 4.335 ± 0.481
2.641SerVal: 2.641 ± 0.348
1.694SerTrp: 1.694 ± 0.372
1.744SerTyr: 1.744 ± 0.292
0.0SerXaa: 0.0 ± 0.0
Thr
5.531ThrAla: 5.531 ± 0.727
0.747ThrCys: 0.747 ± 0.227
3.638ThrAsp: 3.638 ± 0.488
4.236ThrGlu: 4.236 ± 0.447
2.292ThrPhe: 2.292 ± 0.313
5.581ThrGly: 5.581 ± 0.593
1.246ThrHis: 1.246 ± 0.202
3.488ThrIle: 3.488 ± 0.43
2.84ThrLys: 2.84 ± 0.386
3.787ThrLeu: 3.787 ± 0.478
0.997ThrMet: 0.997 ± 0.196
2.292ThrAsn: 2.292 ± 0.36
3.438ThrPro: 3.438 ± 0.365
1.644ThrGln: 1.644 ± 0.319
2.691ThrArg: 2.691 ± 0.473
3.937ThrSer: 3.937 ± 0.445
3.438ThrThr: 3.438 ± 0.538
3.737ThrVal: 3.737 ± 0.437
1.445ThrTrp: 1.445 ± 0.268
1.794ThrTyr: 1.794 ± 0.234
0.0ThrXaa: 0.0 ± 0.0
Val
5.681ValAla: 5.681 ± 0.57
0.797ValCys: 0.797 ± 0.213
3.986ValAsp: 3.986 ± 0.48
4.535ValGlu: 4.535 ± 0.543
1.744ValPhe: 1.744 ± 0.301
5.78ValGly: 5.78 ± 0.577
1.246ValHis: 1.246 ± 0.306
2.641ValIle: 2.641 ± 0.322
3.638ValLys: 3.638 ± 0.448
4.933ValLeu: 4.933 ± 0.519
1.146ValMet: 1.146 ± 0.223
2.043ValAsn: 2.043 ± 0.352
3.388ValPro: 3.388 ± 0.323
2.043ValGln: 2.043 ± 0.335
3.887ValArg: 3.887 ± 0.511
2.89ValSer: 2.89 ± 0.418
3.837ValThr: 3.837 ± 0.511
4.485ValVal: 4.485 ± 0.536
1.146ValTrp: 1.146 ± 0.289
2.591ValTyr: 2.591 ± 0.274
0.0ValXaa: 0.0 ± 0.0
Trp
1.595TrpAla: 1.595 ± 0.328
0.249TrpCys: 0.249 ± 0.103
1.196TrpAsp: 1.196 ± 0.272
1.146TrpGlu: 1.146 ± 0.236
0.498TrpPhe: 0.498 ± 0.219
1.345TrpGly: 1.345 ± 0.284
0.498TrpHis: 0.498 ± 0.136
1.146TrpIle: 1.146 ± 0.265
0.947TrpLys: 0.947 ± 0.206
1.395TrpLeu: 1.395 ± 0.241
0.249TrpMet: 0.249 ± 0.135
0.847TrpAsn: 0.847 ± 0.249
0.747TrpPro: 0.747 ± 0.227
0.897TrpGln: 0.897 ± 0.203
1.694TrpArg: 1.694 ± 0.286
1.146TrpSer: 1.146 ± 0.259
1.694TrpThr: 1.694 ± 0.236
1.495TrpVal: 1.495 ± 0.257
0.149TrpTrp: 0.149 ± 0.086
0.448TrpTyr: 0.448 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.342TyrAla: 2.342 ± 0.357
0.498TyrCys: 0.498 ± 0.145
2.492TyrAsp: 2.492 ± 0.448
1.894TyrGlu: 1.894 ± 0.328
0.698TyrPhe: 0.698 ± 0.192
2.641TyrGly: 2.641 ± 0.39
0.648TyrHis: 0.648 ± 0.179
1.296TyrIle: 1.296 ± 0.245
1.296TyrLys: 1.296 ± 0.259
2.392TyrLeu: 2.392 ± 0.291
0.548TyrMet: 0.548 ± 0.133
1.395TyrAsn: 1.395 ± 0.198
1.445TyrPro: 1.445 ± 0.276
1.196TyrGln: 1.196 ± 0.247
2.89TyrArg: 2.89 ± 0.409
1.794TyrSer: 1.794 ± 0.378
1.694TyrThr: 1.694 ± 0.314
2.143TyrVal: 2.143 ± 0.32
0.598TyrTrp: 0.598 ± 0.193
0.947TyrTyr: 0.947 ± 0.247
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 81 proteins (20069 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski