Bovine gammaherpesvirus 6
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A060CTX9|A0A060CTX9_9GAMA Interleukin-10 domain protein OS=Bovine gammaherpesvirus 6 OX=1504288 GN=Bov2.5 PE=4 SV=1
MM1 pKa = 7.52 GSSSYY6 pKa = 10.18 CQNSFHH12 pKa = 7.23 YY13 pKa = 9.48 EE14 pKa = 3.94 HH15 pKa = 6.54 QCAHH19 pKa = 5.98 SSKK22 pKa = 10.81 DD23 pKa = 2.77 IWLEE27 pKa = 3.83 NQRR30 pKa = 11.84 SFVNRR35 pKa = 11.84 SPLEE39 pKa = 4.08 SQNLANFLYY48 pKa = 10.5 DD49 pKa = 4.41 DD50 pKa = 4.16 SDD52 pKa = 3.98 FSFSEE57 pKa = 4.51 FEE59 pKa = 4.2 QNSTIASFDD68 pKa = 3.54 SLLCDD73 pKa = 4.31 DD74 pKa = 4.98 VSTNMDD80 pKa = 3.36 TNMDD84 pKa = 4.43 EE85 pKa = 5.63 LPLASDD91 pKa = 3.53 NQDD94 pKa = 4.09 AISLSLDD101 pKa = 3.59 PGAKK105 pKa = 9.34 PCSTEE110 pKa = 3.64 KK111 pKa = 10.67 LPQIRR116 pKa = 11.84 RR117 pKa = 11.84 GIDD120 pKa = 3.25 YY121 pKa = 11.04 SLVEE125 pKa = 4.36 SLDD128 pKa = 3.82 TCSSGQLNNKK138 pKa = 7.52 NCKK141 pKa = 9.16 PVSNEE146 pKa = 3.39 QLCFGTCFTTILKK159 pKa = 8.96 QLCFFF164 pKa = 5.33
Molecular weight: 18.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.011
IPC2_protein 4.253
IPC_protein 4.202
Toseland 3.999
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.101
Rodwell 4.037
Grimsley 3.91
Solomon 4.177
Lehninger 4.126
Nozaki 4.291
DTASelect 4.52
Thurlkill 4.05
EMBOSS 4.113
Sillero 4.317
Patrickios 0.846
IPC_peptide 4.177
IPC2_peptide 4.304
IPC2.peptide.svr19 4.198
Protein with the highest isoelectric point:
>tr|A0A060D3N3|A0A060D3N3_9GAMA Major capsid protein OS=Bovine gammaherpesvirus 6 OX=1504288 GN=MCP PE=3 SV=1
MM1 pKa = 7.18 YY2 pKa = 9.9 RR3 pKa = 11.84 YY4 pKa = 9.39 QADD7 pKa = 3.38 KK8 pKa = 11.29 GIVFALSGLPIKK20 pKa = 10.67 RR21 pKa = 11.84 KK22 pKa = 9.25 VEE24 pKa = 4.02 GVKK27 pKa = 10.18 AHH29 pKa = 5.46 QKK31 pKa = 10.51 VYY33 pKa = 10.54 TKK35 pKa = 10.78 LLQFTTFTKK44 pKa = 10.61 LSKK47 pKa = 10.38 FLGIIHH53 pKa = 7.02 PCPCKK58 pKa = 10.84 VKK60 pKa = 10.28 FKK62 pKa = 11.17 LFFEE66 pKa = 4.52 VTLGNRR72 pKa = 11.84 IADD75 pKa = 3.88 CVVLAVQGEE84 pKa = 4.08 NRR86 pKa = 11.84 VCYY89 pKa = 10.01 VVEE92 pKa = 5.8 LKK94 pKa = 9.69 TCMNNTFALSAVQSAQMLQGLAQLSDD120 pKa = 3.26 STKK123 pKa = 10.95 YY124 pKa = 10.54 LFQNAPVDD132 pKa = 3.87 NQNWTIVAHH141 pKa = 6.72 LVFKK145 pKa = 10.71 SQSSLKK151 pKa = 8.9 TIHH154 pKa = 6.36 VEE156 pKa = 3.86 QPKK159 pKa = 10.73 LIFSSMLTRR168 pKa = 11.84 VDD170 pKa = 3.08 KK171 pKa = 11.07 LAAFLHH177 pKa = 5.39 TRR179 pKa = 11.84 EE180 pKa = 4.53 DD181 pKa = 3.23 ATFRR185 pKa = 11.84 QLLRR189 pKa = 11.84 SNKK192 pKa = 9.7 AIKK195 pKa = 9.95 EE196 pKa = 3.96 VAKK199 pKa = 10.27 RR200 pKa = 11.84 KK201 pKa = 9.42 RR202 pKa = 11.84 VLGTKK207 pKa = 10.1 SKK209 pKa = 10.91 ARR211 pKa = 11.84 AQNKK215 pKa = 8.75 PPQNPRR221 pKa = 11.84 VQKK224 pKa = 10.48 KK225 pKa = 9.2 RR226 pKa = 11.84 ALVQKK231 pKa = 10.14 ARR233 pKa = 11.84 CRR235 pKa = 11.84 EE236 pKa = 3.9
Molecular weight: 26.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.217
IPC2_protein 9.575
IPC_protein 9.589
Toseland 10.599
ProMoST 10.087
Dawson 10.687
Bjellqvist 10.277
Wikipedia 10.789
Rodwell 11.301
Grimsley 10.716
Solomon 10.716
Lehninger 10.701
Nozaki 10.584
DTASelect 10.277
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.599
Patrickios 11.008
IPC_peptide 10.73
IPC2_peptide 8.99
IPC2.peptide.svr19 8.53
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
79
0
79
35266
20
2614
446.4
50.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.511 ± 0.185
2.6 ± 0.183
4.358 ± 0.127
5.745 ± 0.238
5.254 ± 0.18
4.307 ± 0.176
2.572 ± 0.084
5.674 ± 0.151
6.474 ± 0.218
10.302 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.131
5.405 ± 0.146
4.979 ± 0.189
4.616 ± 0.213
3.527 ± 0.176
8.654 ± 0.251
5.717 ± 0.238
6.335 ± 0.222
1.08 ± 0.066
3.669 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here