Roseicitreum antarcticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Roseicitreum

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4002 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3BRP9|A0A1H3BRP9_9RHOB Lipoprotein-releasing system ATP-binding protein LolD OS=Roseicitreum antarcticum OX=564137 GN=lolD PE=3 SV=1
MM1 pKa = 6.29QTKK4 pKa = 10.09LYY6 pKa = 10.88AGVATLALIAGSASAQDD23 pKa = 3.67LVFGVGEE30 pKa = 4.17DD31 pKa = 4.78ARR33 pKa = 11.84MNWDD37 pKa = 3.44SYY39 pKa = 12.11NEE41 pKa = 3.75FAEE44 pKa = 4.82ANDD47 pKa = 3.9FSGQSVSLLGPWTGRR62 pKa = 11.84DD63 pKa = 3.39AEE65 pKa = 4.71LVNSTLAYY73 pKa = 9.76FAEE76 pKa = 4.51ATGATVNYY84 pKa = 9.53SGSDD88 pKa = 3.17SFEE91 pKa = 3.78QDD93 pKa = 2.48IVIAAQAGSAPNIAIFPQPGLAADD117 pKa = 4.14MASSGYY123 pKa = 10.63LSALDD128 pKa = 4.27EE129 pKa = 4.53STGDD133 pKa = 3.37WVRR136 pKa = 11.84EE137 pKa = 3.72NYY139 pKa = 10.1AAGEE143 pKa = 4.26SWVDD147 pKa = 3.17LGTYY151 pKa = 10.28AGADD155 pKa = 3.95GEE157 pKa = 4.49DD158 pKa = 3.43GLYY161 pKa = 11.04GFFYY165 pKa = 10.7KK166 pKa = 10.44VDD168 pKa = 3.56VKK170 pKa = 11.34SLVWYY175 pKa = 10.27NPEE178 pKa = 3.67QFFEE182 pKa = 4.22AGYY185 pKa = 10.01DD186 pKa = 3.32IPEE189 pKa = 4.18SMEE192 pKa = 4.05DD193 pKa = 3.43LKK195 pKa = 11.44ALSEE199 pKa = 4.19QIVADD204 pKa = 5.46GGTPWCLGLGSGAATGWPATDD225 pKa = 3.12WVEE228 pKa = 5.2DD229 pKa = 3.44MMLRR233 pKa = 11.84LHH235 pKa = 6.33TPDD238 pKa = 5.38VYY240 pKa = 11.36DD241 pKa = 3.22GWVSNEE247 pKa = 3.62IAFDD251 pKa = 3.73DD252 pKa = 4.12PRR254 pKa = 11.84VVEE257 pKa = 4.96AIEE260 pKa = 4.44AYY262 pKa = 10.66GEE264 pKa = 3.96FSRR267 pKa = 11.84NNDD270 pKa = 2.79WVQGGSEE277 pKa = 3.97AAATTDD283 pKa = 4.27FRR285 pKa = 11.84DD286 pKa = 3.61SPGGLFSIPPACYY299 pKa = 8.41MHH301 pKa = 6.63KK302 pKa = 9.78QASFIPTFFPEE313 pKa = 4.11DD314 pKa = 3.27AEE316 pKa = 4.42YY317 pKa = 11.33GLDD320 pKa = 3.35YY321 pKa = 11.43DD322 pKa = 4.87FFYY325 pKa = 11.14FPASAEE331 pKa = 4.13ADD333 pKa = 3.16LGAPVLGAGTLFAITDD349 pKa = 3.75DD350 pKa = 3.88SDD352 pKa = 3.35ATRR355 pKa = 11.84GLMEE359 pKa = 4.42FLKK362 pKa = 10.27TPIAHH367 pKa = 7.05EE368 pKa = 3.41IWMAQSGFLTPHH380 pKa = 6.26TGVNSEE386 pKa = 4.42AYY388 pKa = 10.43GNDD391 pKa = 3.3SLRR394 pKa = 11.84AMGDD398 pKa = 2.82ILLNATTFRR407 pKa = 11.84FDD409 pKa = 5.1ASDD412 pKa = 3.81LMPGEE417 pKa = 4.37IGAGAFWTGMVDD429 pKa = 3.45YY430 pKa = 7.64TTGADD435 pKa = 3.52ADD437 pKa = 4.31SVATTIQEE445 pKa = 3.6RR446 pKa = 11.84WNAMRR451 pKa = 5.25

Molecular weight: 48.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H2X6D9|A0A1H2X6D9_9RHOB Ribonuclease P protein component OS=Roseicitreum antarcticum OX=564137 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.83HH14 pKa = 4.64RR15 pKa = 11.84HH16 pKa = 3.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27AGQKK29 pKa = 9.71IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.3KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4002

0

4002

1234146

39

4479

308.4

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.333 ± 0.055

0.864 ± 0.012

6.092 ± 0.031

5.02 ± 0.041

3.564 ± 0.026

8.774 ± 0.036

2.123 ± 0.02

4.995 ± 0.034

2.512 ± 0.03

10.13 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.021

2.414 ± 0.019

5.371 ± 0.031

3.382 ± 0.021

7.106 ± 0.039

4.978 ± 0.026

5.692 ± 0.027

7.326 ± 0.034

1.397 ± 0.017

2.069 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski