Pelargonium line pattern virus
Average proteome isoelectric point is 8.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5XKN5|U5XKN5_9TOMB p9.7 OS=Pelargonium line pattern virus OX=167019 GN=p9.7 PE=4 SV=1
MM1 pKa = 7.89 EE2 pKa = 4.57 YY3 pKa = 10.27 PRR5 pKa = 11.84 VHH7 pKa = 6.62 LAILSVLISSQLLIKK22 pKa = 9.6 WNLWSISISDD32 pKa = 4.8 FLPQPHH38 pKa = 6.55 SLHH41 pKa = 6.85 PNLLVCIVLCIFFSSVLSQGQSYY64 pKa = 10.14 SYY66 pKa = 11.21 SYY68 pKa = 11.15 FSTSTSDD75 pKa = 3.02 KK76 pKa = 10.0 FISVAVGNGGQGG88 pKa = 2.97
Molecular weight: 9.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.667
IPC2_protein 6.287
IPC_protein 6.693
Toseland 6.737
ProMoST 6.985
Dawson 7.132
Bjellqvist 6.795
Wikipedia 7.117
Rodwell 7.102
Grimsley 6.81
Solomon 7.234
Lehninger 7.249
Nozaki 7.176
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.512
Sillero 7.483
Patrickios 0.54
IPC_peptide 7.234
IPC2_peptide 7.22
IPC2.peptide.svr19 7.202
Protein with the highest isoelectric point:
>tr|U5XKN5|U5XKN5_9TOMB p9.7 OS=Pelargonium line pattern virus OX=167019 GN=p9.7 PE=4 SV=1
MM1 pKa = 7.24 AHH3 pKa = 6.24 YY4 pKa = 10.09 FGEE7 pKa = 4.27 ALQLGLFVAKK17 pKa = 10.43 ASCWASKK24 pKa = 10.32 EE25 pKa = 3.91 LALLGPNLAWIATRR39 pKa = 11.84 PLRR42 pKa = 11.84 NDD44 pKa = 2.62 ANAILKK50 pKa = 10.38 RR51 pKa = 11.84 IGVHH55 pKa = 6.09 GLAKK59 pKa = 10.11 IPCNFDD65 pKa = 3.31 SDD67 pKa = 4.21 EE68 pKa = 4.01 LTIANDD74 pKa = 4.52 CIVKK78 pKa = 10.05 SDD80 pKa = 3.91 RR81 pKa = 11.84 NLEE84 pKa = 4.16 DD85 pKa = 3.23 EE86 pKa = 4.77 SIVVEE91 pKa = 4.23 EE92 pKa = 4.31 VEE94 pKa = 4.27 EE95 pKa = 4.74 GEE97 pKa = 4.29 EE98 pKa = 4.02 KK99 pKa = 10.62 KK100 pKa = 10.55 KK101 pKa = 10.75 SKK103 pKa = 10.39 KK104 pKa = 5.49 VTRR107 pKa = 11.84 RR108 pKa = 11.84 RR109 pKa = 11.84 VKK111 pKa = 9.8 TKK113 pKa = 8.61 PTFAAVLAADD123 pKa = 3.97 AKK125 pKa = 10.77 NYY127 pKa = 9.63 YY128 pKa = 10.16 GCLPSATRR136 pKa = 11.84 ANEE139 pKa = 3.92 LSVMKK144 pKa = 10.33 YY145 pKa = 10.09 LVSKK149 pKa = 10.14 CQEE152 pKa = 3.84 HH153 pKa = 7.16 KK154 pKa = 10.55 LTITQTRR161 pKa = 11.84 EE162 pKa = 3.4 VSAMAFALTFTPDD175 pKa = 3.24 EE176 pKa = 4.14 NDD178 pKa = 3.19 KK179 pKa = 11.35 LIYY182 pKa = 10.08 KK183 pKa = 8.8 YY184 pKa = 11.06 LNSTEE189 pKa = 4.09 VFEE192 pKa = 5.68 RR193 pKa = 11.84 RR194 pKa = 11.84 VDD196 pKa = 3.49 YY197 pKa = 11.25 AKK199 pKa = 10.97 ARR201 pKa = 11.84 GVDD204 pKa = 3.48 KK205 pKa = 11.01 CWFEE209 pKa = 3.79 LLKK212 pKa = 10.83 RR213 pKa = 11.84 PWHH216 pKa = 5.1 ARR218 pKa = 11.84 AWRR221 pKa = 11.84 RR222 pKa = 11.84 VVGRR226 pKa = 11.84 IFGLPEE232 pKa = 3.33 QQAFEE237 pKa = 4.2 FVKK240 pKa = 9.48 XGCLMDD246 pKa = 3.51 TCGVDD251 pKa = 3.08 TKK253 pKa = 10.75 VYY255 pKa = 9.7 RR256 pKa = 11.84 GEE258 pKa = 4.04 HH259 pKa = 4.88 RR260 pKa = 11.84 WVKK263 pKa = 9.32 EE264 pKa = 3.48 FRR266 pKa = 11.84 GAANPKK272 pKa = 9.12 PRR274 pKa = 11.84 RR275 pKa = 11.84 LYY277 pKa = 10.3 KK278 pKa = 10.25 ISGVSPEE285 pKa = 4.3 VRR287 pKa = 11.84 WGVHH291 pKa = 4.89 NNSFVNLRR299 pKa = 11.84 RR300 pKa = 11.84 GLMEE304 pKa = 3.41 RR305 pKa = 11.84 VFYY308 pKa = 10.13 VEE310 pKa = 4.83 RR311 pKa = 11.84 SGEE314 pKa = 4.12 LLPCPSPEE322 pKa = 3.79 AGLFKK327 pKa = 10.86 RR328 pKa = 11.84 LFNKK332 pKa = 9.53 VGRR335 pKa = 11.84 RR336 pKa = 11.84 VIRR339 pKa = 11.84 FCGHH343 pKa = 6.88 HH344 pKa = 6.24 SPIPRR349 pKa = 11.84 ASYY352 pKa = 9.84 PGMFQGRR359 pKa = 11.84 KK360 pKa = 5.56 RR361 pKa = 11.84 TIYY364 pKa = 9.64 EE365 pKa = 3.67 NAVRR369 pKa = 11.84 SLVDD373 pKa = 3.11 RR374 pKa = 11.84 PYY376 pKa = 11.01 NIRR379 pKa = 11.84 DD380 pKa = 3.49 SYY382 pKa = 11.74 LKK384 pKa = 10.07 TFVKK388 pKa = 10.58 FEE390 pKa = 4.24 KK391 pKa = 10.63 LDD393 pKa = 3.82 FSKK396 pKa = 11.03 KK397 pKa = 9.08 PDD399 pKa = 3.82 PAPRR403 pKa = 11.84 VIQPRR408 pKa = 11.84 HH409 pKa = 4.77 PRR411 pKa = 11.84 YY412 pKa = 9.36 NVEE415 pKa = 3.88 LGRR418 pKa = 11.84 YY419 pKa = 7.19 LKK421 pKa = 10.46 PFEE424 pKa = 4.32 HH425 pKa = 6.76 FCYY428 pKa = 10.23 KK429 pKa = 10.7 ALDD432 pKa = 4.05 KK433 pKa = 11.12 LWGGPTVMKK442 pKa = 10.44 GYY444 pKa = 8.15 TVEE447 pKa = 4.02 EE448 pKa = 3.8 MGGIIKK454 pKa = 10.23 DD455 pKa = 3.12 SWLQFQKK462 pKa = 10.59 PVAIGFDD469 pKa = 3.3 MSRR472 pKa = 11.84 FDD474 pKa = 3.24 QHH476 pKa = 8.4 VSVDD480 pKa = 3.42 ALKK483 pKa = 10.67 FEE485 pKa = 4.16 HH486 pKa = 6.96 SIYY489 pKa = 10.24 KK490 pKa = 10.43 ACFSKK495 pKa = 10.89 DD496 pKa = 3.17 GNLATLLGHH505 pKa = 6.42 QISNRR510 pKa = 11.84 GSAYY514 pKa = 10.57 ANDD517 pKa = 4.32 GYY519 pKa = 11.23 LRR521 pKa = 11.84 YY522 pKa = 9.38 RR523 pKa = 11.84 VEE525 pKa = 4.38 GKK527 pKa = 10.57 RR528 pKa = 11.84 MSGDD532 pKa = 3.26 VNTGLGNCLLACTITKK548 pKa = 10.2 FLMEE552 pKa = 4.89 EE553 pKa = 3.38 IGVRR557 pKa = 11.84 SRR559 pKa = 11.84 LVNNGDD565 pKa = 3.76 DD566 pKa = 3.67 CVLICEE572 pKa = 4.96 AGDD575 pKa = 3.59 CRR577 pKa = 11.84 AVEE580 pKa = 4.35 GSLTVGWRR588 pKa = 11.84 RR589 pKa = 11.84 FGFTCIAEE597 pKa = 4.24 KK598 pKa = 10.15 PVYY601 pKa = 9.24 EE602 pKa = 4.02 LEE604 pKa = 4.78 KK605 pKa = 10.64 IEE607 pKa = 4.29 FCQMSPIQISDD618 pKa = 3.43 SRR620 pKa = 11.84 VKK622 pKa = 10.53 LVRR625 pKa = 11.84 KK626 pKa = 7.02 PQKK629 pKa = 10.54 SISKK633 pKa = 8.91 DD634 pKa = 3.23 AHH636 pKa = 5.36 STTPLTTIQLAQEE649 pKa = 3.78 WTRR652 pKa = 11.84 AIGEE656 pKa = 4.5 GGLSLTSGIPVVQEE670 pKa = 4.63 FYY672 pKa = 11.01 QCLIRR677 pKa = 11.84 NGKK680 pKa = 9.1 KK681 pKa = 8.84 ATKK684 pKa = 9.04 EE685 pKa = 4.05 SKK687 pKa = 10.74 KK688 pKa = 9.02 MAFYY692 pKa = 11.13 GDD694 pKa = 5.06 YY695 pKa = 8.73 YY696 pKa = 9.57 WKK698 pKa = 9.91 WVNQHH703 pKa = 5.2 SGKK706 pKa = 10.34 YY707 pKa = 9.5 EE708 pKa = 3.83 PVTEE712 pKa = 4.17 EE713 pKa = 3.93 ARR715 pKa = 11.84 HH716 pKa = 5.4 SFHH719 pKa = 7.01 LAFGVSPDD727 pKa = 3.49 QQLALEE733 pKa = 5.16 DD734 pKa = 3.38 IYY736 pKa = 11.6 SRR738 pKa = 11.84 KK739 pKa = 9.61 SLLWEE744 pKa = 3.93 SSPFTFEE751 pKa = 3.31 VGAIEE756 pKa = 4.55 WIFNQKK762 pKa = 9.56 IPII765 pKa = 3.98
Molecular weight: 87.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.732
IPC2_protein 8.595
IPC_protein 8.448
Toseland 9.092
ProMoST 9.019
Dawson 9.443
Bjellqvist 9.326
Wikipedia 9.604
Rodwell 9.663
Grimsley 9.472
Solomon 9.472
Lehninger 9.443
Nozaki 9.487
DTASelect 9.209
Thurlkill 9.341
EMBOSS 9.604
Sillero 9.502
Patrickios 4.762
IPC_peptide 9.472
IPC2_peptide 8.068
IPC2.peptide.svr19 7.76
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1621
63
765
270.2
30.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.588 ± 0.984
2.468 ± 0.322
4.257 ± 0.286
6.416 ± 1.019
4.257 ± 0.627
6.971 ± 0.802
2.097 ± 0.2
4.812 ± 0.422
7.65 ± 0.868
8.945 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.604 ± 0.197
3.825 ± 0.291
4.257 ± 0.51
2.714 ± 0.291
6.477 ± 0.915
8.143 ± 2.13
5.12 ± 0.655
7.341 ± 0.588
1.727 ± 0.346
3.27 ± 0.492
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here