Mouse mammary tumor virus (strain C3H) (MMTV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Betaretrovirus; Mouse mammary tumor virus

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9IZT2|PRO_MMTVC Gag-Pro polyprotein OS=Mouse mammary tumor virus (strain C3H) OX=11759 GN=gag-pro PE=1 SV=1
MM1 pKa = 7.24GVSGSKK7 pKa = 9.48GQKK10 pKa = 10.01LFVSVLQRR18 pKa = 11.84LLSEE22 pKa = 4.7RR23 pKa = 11.84GLHH26 pKa = 5.31VKK28 pKa = 10.05EE29 pKa = 4.39SSAIEE34 pKa = 4.43FYY36 pKa = 11.02QFLIKK41 pKa = 10.55VSPWFPEE48 pKa = 3.79EE49 pKa = 4.5GGLNLQDD56 pKa = 3.26WKK58 pKa = 11.05RR59 pKa = 11.84VGRR62 pKa = 11.84EE63 pKa = 3.7MKK65 pKa = 10.24KK66 pKa = 9.98YY67 pKa = 9.71AAEE70 pKa = 4.67HH71 pKa = 5.81GTDD74 pKa = 4.27SIPKK78 pKa = 7.84QAYY81 pKa = 7.84PIWLQLRR88 pKa = 11.84EE89 pKa = 4.17ILTEE93 pKa = 3.88QSDD96 pKa = 4.44LVLLSAEE103 pKa = 4.15AKK105 pKa = 10.18SVTEE109 pKa = 4.08EE110 pKa = 3.97EE111 pKa = 4.47LEE113 pKa = 4.17EE114 pKa = 4.46GLTGLLSASSQEE126 pKa = 3.85KK127 pKa = 9.34TYY129 pKa = 8.97GTRR132 pKa = 11.84GTAYY136 pKa = 10.45AEE138 pKa = 4.13IDD140 pKa = 3.78TEE142 pKa = 4.13VDD144 pKa = 3.01KK145 pKa = 11.4LSEE148 pKa = 4.53HH149 pKa = 7.04IYY151 pKa = 10.55DD152 pKa = 3.66EE153 pKa = 4.71PYY155 pKa = 10.62EE156 pKa = 4.21EE157 pKa = 5.61KK158 pKa = 10.79EE159 pKa = 4.01KK160 pKa = 10.96ADD162 pKa = 3.5KK163 pKa = 11.34NEE165 pKa = 4.22EE166 pKa = 3.82KK167 pKa = 11.02DD168 pKa = 3.3HH169 pKa = 5.17VRR171 pKa = 11.84KK172 pKa = 9.7VKK174 pKa = 10.63KK175 pKa = 9.75IVQRR179 pKa = 11.84KK180 pKa = 7.92EE181 pKa = 3.59NSEE184 pKa = 4.13HH185 pKa = 6.14KK186 pKa = 10.44RR187 pKa = 11.84KK188 pKa = 10.3EE189 pKa = 3.84KK190 pKa = 10.28DD191 pKa = 3.01QKK193 pKa = 11.64AFLATDD199 pKa = 3.82WNNDD203 pKa = 3.68DD204 pKa = 5.69LSPEE208 pKa = 4.01DD209 pKa = 3.7WDD211 pKa = 4.93DD212 pKa = 4.49LEE214 pKa = 4.4EE215 pKa = 4.25QAAHH219 pKa = 5.73YY220 pKa = 10.51HH221 pKa = 7.21DD222 pKa = 5.91DD223 pKa = 4.67DD224 pKa = 4.55EE225 pKa = 7.21LILPVKK231 pKa = 10.53RR232 pKa = 11.84KK233 pKa = 9.02VDD235 pKa = 3.44KK236 pKa = 10.82KK237 pKa = 11.02KK238 pKa = 10.47PLALRR243 pKa = 11.84RR244 pKa = 11.84KK245 pKa = 8.39PLPPVGFAGAMAEE258 pKa = 3.92ARR260 pKa = 11.84EE261 pKa = 4.19KK262 pKa = 11.38GDD264 pKa = 3.29LTFTFPVVFMGEE276 pKa = 4.1SDD278 pKa = 5.49DD279 pKa = 5.77DD280 pKa = 4.05DD281 pKa = 4.37TPVWEE286 pKa = 4.43PLPLKK291 pKa = 8.8TLKK294 pKa = 10.12EE295 pKa = 4.02LQSAVRR301 pKa = 11.84TMGPSAPYY309 pKa = 8.48TLQVVDD315 pKa = 4.68MVASQWLTPSDD326 pKa = 2.96WHH328 pKa = 4.57QTARR332 pKa = 11.84ATLSPGDD339 pKa = 3.64YY340 pKa = 10.37VLWRR344 pKa = 11.84TEE346 pKa = 3.81YY347 pKa = 10.48EE348 pKa = 4.28EE349 pKa = 4.34KK350 pKa = 10.82SKK352 pKa = 9.72EE353 pKa = 4.5TVQKK357 pKa = 9.56TAGKK361 pKa = 9.84RR362 pKa = 11.84KK363 pKa = 9.95GKK365 pKa = 10.54VSLDD369 pKa = 3.39MLLGTGQFLSPSSQIKK385 pKa = 10.11LSKK388 pKa = 10.67DD389 pKa = 3.14VLKK392 pKa = 10.93DD393 pKa = 3.24VTTNAVLAWRR403 pKa = 11.84AIPPPGVKK411 pKa = 8.73KK412 pKa = 9.08TVLAGLKK419 pKa = 9.73QGNEE423 pKa = 3.61EE424 pKa = 4.18SYY426 pKa = 9.06EE427 pKa = 4.17TFISRR432 pKa = 11.84LEE434 pKa = 3.95EE435 pKa = 3.46AVYY438 pKa = 11.09RR439 pKa = 11.84MMPRR443 pKa = 11.84GEE445 pKa = 4.33GSDD448 pKa = 3.43ILIKK452 pKa = 10.43QLAWEE457 pKa = 4.39NANSLCQDD465 pKa = 5.01LIRR468 pKa = 11.84PMRR471 pKa = 11.84KK472 pKa = 7.29TGTMQDD478 pKa = 3.89YY479 pKa = 10.81IRR481 pKa = 11.84ACLDD485 pKa = 3.11ASPAVVQGMAYY496 pKa = 9.19AAAMRR501 pKa = 11.84GQKK504 pKa = 10.41YY505 pKa = 7.44STFVKK510 pKa = 8.72QTYY513 pKa = 10.18GGGKK517 pKa = 9.54GGQGSEE523 pKa = 4.33GPVCFSCGKK532 pKa = 8.11TGHH535 pKa = 6.88IKK537 pKa = 10.2RR538 pKa = 11.84DD539 pKa = 3.61CKK541 pKa = 10.52EE542 pKa = 3.87EE543 pKa = 4.15KK544 pKa = 10.01GSKK547 pKa = 9.49RR548 pKa = 11.84APPGLCPRR556 pKa = 11.84CKK558 pKa = 10.06KK559 pKa = 10.1GYY561 pKa = 8.72HH562 pKa = 5.97WKK564 pKa = 10.34SEE566 pKa = 4.25CKK568 pKa = 10.4SKK570 pKa = 10.64FDD572 pKa = 4.67KK573 pKa = 11.01DD574 pKa = 4.08GNPLPPLEE582 pKa = 4.45TNAEE586 pKa = 3.94NSKK589 pKa = 10.69NLVKK593 pKa = 10.6GQSPSPTQKK602 pKa = 10.56GDD604 pKa = 2.96KK605 pKa = 10.95GKK607 pKa = 10.56DD608 pKa = 2.96SGLNPEE614 pKa = 4.78APPFTIHH621 pKa = 7.2DD622 pKa = 4.52LPRR625 pKa = 11.84GTPGSAGLDD634 pKa = 3.34LSSQKK639 pKa = 11.1DD640 pKa = 3.83LILSLEE646 pKa = 4.66DD647 pKa = 3.62GVSLVPTLVKK657 pKa = 9.96GTLPEE662 pKa = 4.4GTTGLIIGRR671 pKa = 11.84SSNYY675 pKa = 9.95KK676 pKa = 10.09KK677 pKa = 10.73GLEE680 pKa = 3.98VLPGVIDD687 pKa = 3.78SDD689 pKa = 3.88FQGEE693 pKa = 4.13IKK695 pKa = 11.16VMVKK699 pKa = 9.89AAKK702 pKa = 9.95NAVIIHH708 pKa = 5.75KK709 pKa = 9.37GEE711 pKa = 4.84RR712 pKa = 11.84IAQLLLLPYY721 pKa = 10.27LKK723 pKa = 10.6LPNPIIKK730 pKa = 9.82EE731 pKa = 4.0EE732 pKa = 4.09RR733 pKa = 11.84GSEE736 pKa = 4.39GFGSTSHH743 pKa = 5.12VHH745 pKa = 5.11WVQEE749 pKa = 4.27ISDD752 pKa = 4.49SRR754 pKa = 11.84PMLHH758 pKa = 6.63ISLNGRR764 pKa = 11.84RR765 pKa = 11.84FLGLLDD771 pKa = 3.56TGADD775 pKa = 3.57KK776 pKa = 10.91TCIAGRR782 pKa = 11.84DD783 pKa = 3.7WPANWPIHH791 pKa = 4.59QTEE794 pKa = 4.39SSLQGLGMACGVARR808 pKa = 11.84SSQPLRR814 pKa = 11.84WQHH817 pKa = 5.4EE818 pKa = 4.34DD819 pKa = 2.71KK820 pKa = 11.25SGIIHH825 pKa = 6.86PFVIPTLPFTLWGRR839 pKa = 11.84DD840 pKa = 2.7IMKK843 pKa = 9.72EE844 pKa = 3.58IKK846 pKa = 9.82VRR848 pKa = 11.84LMTDD852 pKa = 3.18SPDD855 pKa = 3.91DD856 pKa = 3.91SQDD859 pKa = 3.16LL860 pKa = 3.79

Molecular weight: 95.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P11283|POL_MMTVC Gag-Pro-Pol polyprotein OS=Mouse mammary tumor virus (strain C3H) OX=11759 GN=gag-pro-pol PE=1 SV=2
MM1 pKa = 7.81PRR3 pKa = 11.84LQQKK7 pKa = 8.42WLNSRR12 pKa = 11.84EE13 pKa = 4.26CPTPRR18 pKa = 11.84GEE20 pKa = 4.01AAKK23 pKa = 10.8GLFPTKK29 pKa = 10.09DD30 pKa = 3.35DD31 pKa = 4.04PSAHH35 pKa = 6.33KK36 pKa = 10.28RR37 pKa = 11.84VSPSDD42 pKa = 3.17KK43 pKa = 10.83DD44 pKa = 4.33IFILCCKK51 pKa = 10.39LGIALLCLGLLGEE64 pKa = 4.43VAVRR68 pKa = 11.84ARR70 pKa = 11.84RR71 pKa = 11.84ALTLDD76 pKa = 3.59SFNSSSVQDD85 pKa = 3.67YY86 pKa = 11.05NLNNSEE92 pKa = 4.01NSTFLLRR99 pKa = 11.84QGPQPTSSYY108 pKa = 10.03KK109 pKa = 9.43PHH111 pKa = 7.07RR112 pKa = 11.84FCPSEE117 pKa = 3.68IEE119 pKa = 3.97IRR121 pKa = 11.84MLAKK125 pKa = 10.53NYY127 pKa = 10.11IFTNKK132 pKa = 8.61TNPIGRR138 pKa = 11.84LLVTMLRR145 pKa = 11.84NEE147 pKa = 4.22SLSFSTIFTQIQKK160 pKa = 10.9LEE162 pKa = 3.86MGIEE166 pKa = 3.83NRR168 pKa = 11.84KK169 pKa = 9.38RR170 pKa = 11.84RR171 pKa = 11.84STSIEE176 pKa = 3.86EE177 pKa = 4.07QVQGLLTTGLEE188 pKa = 4.15VKK190 pKa = 10.08KK191 pKa = 10.57GKK193 pKa = 10.36KK194 pKa = 9.59SVFVKK199 pKa = 10.43IGDD202 pKa = 3.81RR203 pKa = 11.84WWQPGTYY210 pKa = 8.74RR211 pKa = 11.84GPYY214 pKa = 9.15IYY216 pKa = 10.27RR217 pKa = 11.84PTDD220 pKa = 3.06APLPYY225 pKa = 9.61TGRR228 pKa = 11.84YY229 pKa = 8.4DD230 pKa = 4.45LNWDD234 pKa = 3.05RR235 pKa = 11.84WVTVNGYY242 pKa = 9.47KK243 pKa = 10.11VLYY246 pKa = 9.63RR247 pKa = 11.84SLPFRR252 pKa = 11.84EE253 pKa = 3.71RR254 pKa = 11.84LARR257 pKa = 11.84ARR259 pKa = 11.84PPWCMLSQEE268 pKa = 5.0EE269 pKa = 4.47KK270 pKa = 11.09DD271 pKa = 3.96DD272 pKa = 3.95MKK274 pKa = 11.16QQVHH278 pKa = 6.95DD279 pKa = 4.61YY280 pKa = 9.71IYY282 pKa = 10.4LGTGMHH288 pKa = 6.45FWGKK292 pKa = 9.73IFHH295 pKa = 6.16TKK297 pKa = 9.89EE298 pKa = 3.66GTVAGLIEE306 pKa = 4.89HH307 pKa = 6.72YY308 pKa = 9.96SAKK311 pKa = 9.69TYY313 pKa = 10.37GMSYY317 pKa = 10.87YY318 pKa = 10.34EE319 pKa = 4.02

Molecular weight: 36.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4213

319

1755

842.6

94.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.29 ± 0.258

1.305 ± 0.11

5.72 ± 0.257

6.694 ± 0.636

3.181 ± 0.373

6.931 ± 0.426

2.421 ± 0.269

4.913 ± 0.467

8.189 ± 0.618

10.207 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.875 ± 0.12

3.038 ± 0.384

6.361 ± 0.189

4.652 ± 0.306

4.937 ± 0.335

6.907 ± 0.21

5.839 ± 0.171

5.673 ± 0.148

2.089 ± 0.054

2.777 ± 0.288
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski