Amino acid dipepetide frequency for Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.068AlaAla: 13.068 ± 0.118	1.284AlaCys: 1.284 ± 0.025	5.795AlaAsp: 5.795 ± 0.056	6.617AlaGlu: 6.617 ± 0.071	3.772AlaPhe: 3.772 ± 0.041	9.265AlaGly: 9.265 ± 0.093	2.164AlaHis: 2.164 ± 0.037	5.025AlaIle: 5.025 ± 0.055	3.461AlaLys: 3.461 ± 0.061	14.369AlaLeu: 14.369 ± 0.108	2.793AlaMet: 2.793 ± 0.041	2.902AlaAsn: 2.902 ± 0.038	5.162AlaPro: 5.162 ± 0.068	5.925AlaGln: 5.925 ± 0.055	7.222AlaArg: 7.222 ± 0.061	6.38AlaSer: 6.38 ± 0.061	4.851AlaThr: 4.851 ± 0.072	7.333AlaVal: 7.333 ± 0.064	1.628AlaTrp: 1.628 ± 0.029	2.625AlaTyr: 2.625 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.188CysAla: 1.188 ± 0.024	0.159CysCys: 0.159 ± 0.009	0.522CysAsp: 0.522 ± 0.015	0.532CysGlu: 0.532 ± 0.016	0.344CysPhe: 0.344 ± 0.014	0.996CysGly: 0.996 ± 0.022	0.269CysHis: 0.269 ± 0.013	0.448CysIle: 0.448 ± 0.014	0.276CysLys: 0.276 ± 0.012	1.242CysLeu: 1.242 ± 0.028	0.217CysMet: 0.217 ± 0.01	0.28CysAsn: 0.28 ± 0.011	0.508CysPro: 0.508 ± 0.016	0.456CysGln: 0.456 ± 0.016	0.65CysArg: 0.65 ± 0.018	0.649CysSer: 0.649 ± 0.018	0.464CysThr: 0.464 ± 0.016	0.67CysVal: 0.67 ± 0.019	0.157CysTrp: 0.157 ± 0.009	0.285CysTyr: 0.285 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	5.352AspAla: 5.352 ± 0.058	0.585AspCys: 0.585 ± 0.015	2.642AspAsp: 2.642 ± 0.044	3.092AspGlu: 3.092 ± 0.044	2.115AspPhe: 2.115 ± 0.035	4.235AspGly: 4.235 ± 0.051	1.114AspHis: 1.114 ± 0.027	2.521AspIle: 2.521 ± 0.042	1.754AspLys: 1.754 ± 0.033	6.016AspLeu: 6.016 ± 0.064	1.11AspMet: 1.11 ± 0.025	1.561AspAsn: 1.561 ± 0.033	2.956AspPro: 2.956 ± 0.037	2.315AspGln: 2.315 ± 0.035	2.954AspArg: 2.954 ± 0.041	3.146AspSer: 3.146 ± 0.04	2.231AspThr: 2.231 ± 0.043	3.153AspVal: 3.153 ± 0.042	0.973AspTrp: 0.973 ± 0.021	1.767AspTyr: 1.767 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	6.215GluAla: 6.215 ± 0.062	0.426GluCys: 0.426 ± 0.015	2.363GluAsp: 2.363 ± 0.033	2.937GluGlu: 2.937 ± 0.047	1.739GluPhe: 1.739 ± 0.032	3.806GluGly: 3.806 ± 0.048	1.759GluHis: 1.759 ± 0.032	2.564GluIle: 2.564 ± 0.038	1.818GluLys: 1.818 ± 0.032	6.798GluLeu: 6.798 ± 0.07	1.174GluMet: 1.174 ± 0.027	1.409GluAsn: 1.409 ± 0.025	2.547GluPro: 2.547 ± 0.036	3.994GluGln: 3.994 ± 0.055	4.604GluArg: 4.604 ± 0.049	2.594GluSer: 2.594 ± 0.038	2.278GluThr: 2.278 ± 0.036	3.969GluVal: 3.969 ± 0.048	0.667GluTrp: 0.667 ± 0.018	1.181GluTyr: 1.181 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.151PheAla: 4.151 ± 0.048	0.488PheCys: 0.488 ± 0.017	2.415PheAsp: 2.415 ± 0.035	1.983PheGlu: 1.983 ± 0.032	1.472PhePhe: 1.472 ± 0.035	3.217PheGly: 3.217 ± 0.045	0.76PheHis: 0.76 ± 0.02	1.846PheIle: 1.846 ± 0.031	1.283PheLys: 1.283 ± 0.026	3.212PheLeu: 3.212 ± 0.045	0.826PheMet: 0.826 ± 0.019	1.357PheAsn: 1.357 ± 0.027	1.477PhePro: 1.477 ± 0.028	1.265PheGln: 1.265 ± 0.025	1.695PheArg: 1.695 ± 0.031	2.673PheSer: 2.673 ± 0.038	1.823PheThr: 1.823 ± 0.032	2.448PheVal: 2.448 ± 0.038	0.592PheTrp: 0.592 ± 0.018	1.059PheTyr: 1.059 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	7.769GlyAla: 7.769 ± 0.077	0.983GlyCys: 0.983 ± 0.023	3.79GlyAsp: 3.79 ± 0.046	4.432GlyGlu: 4.432 ± 0.055	3.433GlyPhe: 3.433 ± 0.039	6.123GlyGly: 6.123 ± 0.082	1.947GlyHis: 1.947 ± 0.03	4.063GlyIle: 4.063 ± 0.049	3.187GlyLys: 3.187 ± 0.041	9.937GlyLeu: 9.937 ± 0.082	2.065GlyMet: 2.065 ± 0.037	2.412GlyAsn: 2.412 ± 0.044	2.879GlyPro: 2.879 ± 0.035	4.196GlyGln: 4.196 ± 0.055	4.985GlyArg: 4.985 ± 0.052	4.922GlySer: 4.922 ± 0.073	3.715GlyThr: 3.715 ± 0.058	5.873GlyVal: 5.873 ± 0.061	1.41GlyTrp: 1.41 ± 0.029	2.551GlyTyr: 2.551 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.153HisAla: 2.153 ± 0.037	0.336HisCys: 0.336 ± 0.013	1.165HisAsp: 1.165 ± 0.028	1.19HisGlu: 1.19 ± 0.027	1.018HisPhe: 1.018 ± 0.023	2.065HisGly: 2.065 ± 0.039	0.632HisHis: 0.632 ± 0.019	0.96HisIle: 0.96 ± 0.025	0.661HisLys: 0.661 ± 0.018	2.769HisLeu: 2.769 ± 0.043	0.501HisMet: 0.501 ± 0.017	0.693HisAsn: 0.693 ± 0.02	1.454HisPro: 1.454 ± 0.028	1.082HisGln: 1.082 ± 0.024	1.31HisArg: 1.31 ± 0.027	1.477HisSer: 1.477 ± 0.029	0.918HisThr: 0.918 ± 0.022	1.246HisVal: 1.246 ± 0.025	0.526HisTrp: 0.526 ± 0.017	0.78HisTyr: 0.78 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.701IleAla: 5.701 ± 0.059	0.52IleCys: 0.52 ± 0.017	3.196IleAsp: 3.196 ± 0.041	3.148IleGlu: 3.148 ± 0.039	1.432IlePhe: 1.432 ± 0.027	4.466IleGly: 4.466 ± 0.058	0.98IleHis: 0.98 ± 0.02	1.976IleIle: 1.976 ± 0.034	1.635IleLys: 1.635 ± 0.028	4.012IleLeu: 4.012 ± 0.052	0.741IleMet: 0.741 ± 0.017	1.707IleAsn: 1.707 ± 0.031	2.178IlePro: 2.178 ± 0.033	1.689IleGln: 1.689 ± 0.028	2.773IleArg: 2.773 ± 0.04	3.072IleSer: 3.072 ± 0.044	2.414IleThr: 2.414 ± 0.049	2.993IleVal: 2.993 ± 0.035	0.521IleTrp: 0.521 ± 0.015	1.118IleTyr: 1.118 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	3.893LysAla: 3.893 ± 0.052	0.168LysCys: 0.168 ± 0.011	1.692LysAsp: 1.692 ± 0.037	1.583LysGlu: 1.583 ± 0.032	0.806LysPhe: 0.806 ± 0.022	2.488LysGly: 2.488 ± 0.046	0.732LysHis: 0.732 ± 0.019	1.534LysIle: 1.534 ± 0.029	1.185LysLys: 1.185 ± 0.035	3.498LysLeu: 3.498 ± 0.043	0.64LysMet: 0.64 ± 0.021	0.993LysAsn: 0.993 ± 0.026	1.978LysPro: 1.978 ± 0.033	1.496LysGln: 1.496 ± 0.03	2.187LysArg: 2.187 ± 0.041	1.75LysSer: 1.75 ± 0.033	1.72LysThr: 1.72 ± 0.033	2.649LysVal: 2.649 ± 0.037	0.329LysTrp: 0.329 ± 0.012	0.677LysTyr: 0.677 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.714LeuAla: 14.714 ± 0.114	1.329LeuCys: 1.329 ± 0.028	6.642LeuAsp: 6.642 ± 0.067	6.755LeuGlu: 6.755 ± 0.067	4.271LeuPhe: 4.271 ± 0.062	9.904LeuGly: 9.904 ± 0.082	2.617LeuHis: 2.617 ± 0.038	5.528LeuIle: 5.528 ± 0.056	4.314LeuLys: 4.314 ± 0.056	14.729LeuLeu: 14.729 ± 0.135	2.587LeuMet: 2.587 ± 0.041	3.789LeuAsn: 3.789 ± 0.047	6.612LeuPro: 6.612 ± 0.061	5.585LeuGln: 5.585 ± 0.073	7.468LeuArg: 7.468 ± 0.075	7.799LeuSer: 7.799 ± 0.072	5.556LeuThr: 5.556 ± 0.054	7.805LeuVal: 7.805 ± 0.078	1.503LeuTrp: 1.503 ± 0.034	2.684LeuTyr: 2.684 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.559MetAla: 2.559 ± 0.037	0.163MetCys: 0.163 ± 0.009	1.005MetAsp: 1.005 ± 0.024	0.879MetGlu: 0.879 ± 0.024	0.681MetPhe: 0.681 ± 0.021	1.636MetGly: 1.636 ± 0.03	0.519MetHis: 0.519 ± 0.015	1.1MetIle: 1.1 ± 0.022	0.746MetLys: 0.746 ± 0.022	2.649MetLeu: 2.649 ± 0.04	0.434MetMet: 0.434 ± 0.014	0.832MetAsn: 0.832 ± 0.022	1.317MetPro: 1.317 ± 0.025	1.056MetGln: 1.056 ± 0.022	1.361MetArg: 1.361 ± 0.032	1.713MetSer: 1.713 ± 0.029	1.347MetThr: 1.347 ± 0.028	1.44MetVal: 1.44 ± 0.028	0.176MetTrp: 0.176 ± 0.009	0.347MetTyr: 0.347 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.145AsnAla: 3.145 ± 0.043	0.296AsnCys: 0.296 ± 0.012	1.505AsnAsp: 1.505 ± 0.028	1.343AsnGlu: 1.343 ± 0.029	1.047AsnPhe: 1.047 ± 0.028	2.55AsnGly: 2.55 ± 0.053	0.614AsnHis: 0.614 ± 0.02	1.381AsnIle: 1.381 ± 0.027	0.875AsnLys: 0.875 ± 0.024	3.613AsnLeu: 3.613 ± 0.046	0.554AsnMet: 0.554 ± 0.018	0.969AsnAsn: 0.969 ± 0.026	2.027AsnPro: 2.027 ± 0.03	1.42AsnGln: 1.42 ± 0.033	1.864AsnArg: 1.864 ± 0.031	1.714AsnSer: 1.714 ± 0.033	1.445AsnThr: 1.445 ± 0.028	1.847AsnVal: 1.847 ± 0.032	0.487AsnTrp: 0.487 ± 0.016	0.826AsnTyr: 0.826 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	6.052ProAla: 6.052 ± 0.076	0.403ProCys: 0.403 ± 0.014	2.688ProAsp: 2.688 ± 0.042	3.267ProGlu: 3.267 ± 0.041	1.768ProPhe: 1.768 ± 0.034	4.338ProGly: 4.338 ± 0.048	1.094ProHis: 1.094 ± 0.024	1.974ProIle: 1.974 ± 0.033	1.54ProLys: 1.54 ± 0.035	6.183ProLeu: 6.183 ± 0.074	1.172ProMet: 1.172 ± 0.027	1.297ProAsn: 1.297 ± 0.026	2.04ProPro: 2.04 ± 0.037	2.852ProGln: 2.852 ± 0.045	2.762ProArg: 2.762 ± 0.042	2.778ProSer: 2.778 ± 0.036	2.147ProThr: 2.147 ± 0.03	3.735ProVal: 3.735 ± 0.053	0.808ProTrp: 0.808 ± 0.02	1.209ProTyr: 1.209 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	6.765GlnAla: 6.765 ± 0.082	0.385GlnCys: 0.385 ± 0.014	2.026GlnAsp: 2.026 ± 0.034	2.129GlnGlu: 2.129 ± 0.035	1.432GlnPhe: 1.432 ± 0.027	4.065GlnGly: 4.065 ± 0.057	1.254GlnHis: 1.254 ± 0.025	2.19GlnIle: 2.19 ± 0.036	1.228GlnLys: 1.228 ± 0.029	6.381GlnLeu: 6.381 ± 0.063	1.096GlnMet: 1.096 ± 0.024	1.149GlnAsn: 1.149 ± 0.024	2.764GlnPro: 2.764 ± 0.038	3.393GlnGln: 3.393 ± 0.058	4.135GlnArg: 4.135 ± 0.05	2.518GlnSer: 2.518 ± 0.041	1.955GlnThr: 1.955 ± 0.032	4.073GlnVal: 4.073 ± 0.043	0.828GlnTrp: 0.828 ± 0.02	1.053GlnTyr: 1.053 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	5.95ArgAla: 5.95 ± 0.049	0.639ArgCys: 0.639 ± 0.019	3.384ArgAsp: 3.384 ± 0.041	4.01ArgGlu: 4.01 ± 0.047	2.595ArgPhe: 2.595 ± 0.037	4.081ArgGly: 4.081 ± 0.042	1.782ArgHis: 1.782 ± 0.031	3.263ArgIle: 3.263 ± 0.043	1.933ArgLys: 1.933 ± 0.032	8.654ArgLeu: 8.654 ± 0.075	1.525ArgMet: 1.525 ± 0.026	1.95ArgAsn: 1.95 ± 0.029	2.913ArgPro: 2.913 ± 0.036	3.895ArgGln: 3.895 ± 0.052	4.324ArgArg: 4.324 ± 0.062	3.541ArgSer: 3.541 ± 0.046	2.62ArgThr: 2.62 ± 0.034	4.355ArgVal: 4.355 ± 0.045	1.087ArgTrp: 1.087 ± 0.024	2.041ArgTyr: 2.041 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.568SerAla: 6.568 ± 0.07	0.51SerCys: 0.51 ± 0.018	2.874SerAsp: 2.874 ± 0.044	2.977SerGlu: 2.977 ± 0.041	2.315SerPhe: 2.315 ± 0.034	5.39SerGly: 5.39 ± 0.068	1.381SerHis: 1.381 ± 0.023	2.853SerIle: 2.853 ± 0.041	1.753SerLys: 1.753 ± 0.032	7.638SerLeu: 7.638 ± 0.071	1.368SerMet: 1.368 ± 0.03	1.797SerAsn: 1.797 ± 0.034	2.866SerPro: 2.866 ± 0.036	2.671SerGln: 2.671 ± 0.043	3.759SerArg: 3.759 ± 0.049	3.78SerSer: 3.78 ± 0.057	2.895SerThr: 2.895 ± 0.045	4.075SerVal: 4.075 ± 0.055	0.86SerTrp: 0.86 ± 0.023	1.574SerTyr: 1.574 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	4.927ThrAla: 4.927 ± 0.066	0.467ThrCys: 0.467 ± 0.015	2.219ThrAsp: 2.219 ± 0.045	2.147ThrGlu: 2.147 ± 0.036	1.668ThrPhe: 1.668 ± 0.032	3.949ThrGly: 3.949 ± 0.059	1.004ThrHis: 1.004 ± 0.025	1.758ThrIle: 1.758 ± 0.054	0.968ThrLys: 0.968 ± 0.024	6.665ThrLeu: 6.665 ± 0.074	0.693ThrMet: 0.693 ± 0.019	1.126ThrAsn: 1.126 ± 0.033	3.071ThrPro: 3.071 ± 0.047	2.106ThrGln: 2.106 ± 0.031	3.094ThrArg: 3.094 ± 0.039	2.608ThrSer: 2.608 ± 0.044	2.216ThrThr: 2.216 ± 0.045	3.239ThrVal: 3.239 ± 0.053	0.697ThrTrp: 0.697 ± 0.017	1.169ThrTyr: 1.169 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	7.722ValAla: 7.722 ± 0.069	0.713ValCys: 0.713 ± 0.018	3.721ValAsp: 3.721 ± 0.045	4.117ValGlu: 4.117 ± 0.053	2.513ValPhe: 2.513 ± 0.04	5.083ValGly: 5.083 ± 0.047	1.353ValHis: 1.353 ± 0.023	3.675ValIle: 3.675 ± 0.043	2.164ValLys: 2.164 ± 0.037	8.258ValLeu: 8.258 ± 0.073	1.615ValMet: 1.615 ± 0.026	2.06ValAsn: 2.06 ± 0.04	3.263ValPro: 3.263 ± 0.04	2.957ValGln: 2.957 ± 0.044	4.179ValArg: 4.179 ± 0.051	4.265ValSer: 4.265 ± 0.05	3.429ValThr: 3.429 ± 0.078	4.996ValVal: 4.996 ± 0.058	0.853ValTrp: 0.853 ± 0.024	1.552ValTyr: 1.552 ± 0.035	0.0ValXaa: 0.0 ± 0.0
Trp	1.233TrpAla: 1.233 ± 0.025	0.158TrpCys: 0.158 ± 0.008	0.613TrpAsp: 0.613 ± 0.017	0.572TrpGlu: 0.572 ± 0.015	0.551TrpPhe: 0.551 ± 0.018	0.952TrpGly: 0.952 ± 0.027	0.395TrpHis: 0.395 ± 0.015	0.669TrpIle: 0.669 ± 0.019	0.469TrpLys: 0.469 ± 0.016	2.361TrpLeu: 2.361 ± 0.045	0.384TrpMet: 0.384 ± 0.013	0.483TrpAsn: 0.483 ± 0.015	0.723TrpPro: 0.723 ± 0.019	1.052TrpGln: 1.052 ± 0.024	1.148TrpArg: 1.148 ± 0.028	0.906TrpSer: 0.906 ± 0.019	0.586TrpThr: 0.586 ± 0.017	0.983TrpVal: 0.983 ± 0.022	0.259TrpTrp: 0.259 ± 0.012	0.327TrpTyr: 0.327 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.521TyrAla: 2.521 ± 0.032	0.299TyrCys: 0.299 ± 0.011	1.337TyrAsp: 1.337 ± 0.029	1.131TyrGlu: 1.131 ± 0.025	1.07TyrPhe: 1.07 ± 0.024	2.06TyrGly: 2.06 ± 0.037	0.564TyrHis: 0.564 ± 0.018	0.944TyrIle: 0.944 ± 0.02	0.744TyrLys: 0.744 ± 0.021	3.206TyrLeu: 3.206 ± 0.042	0.461TyrMet: 0.461 ± 0.015	0.76TyrAsn: 0.76 ± 0.023	1.373TyrPro: 1.373 ± 0.029	1.413TyrGln: 1.413 ± 0.027	2.07TyrArg: 2.07 ± 0.028	1.619TyrSer: 1.619 ± 0.031	1.181TyrThr: 1.181 ± 0.033	1.607TyrVal: 1.607 ± 0.028	0.462TyrTrp: 0.462 ± 0.017	0.728TyrTyr: 0.728 ± 0.022	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6108 proteins (2063158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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