Rhinolophus ferrumequinum circovirus 1
Average proteome isoelectric point is 8.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3VR57|I3VR57_9CIRC Cap OS=Rhinolophus ferrumequinum circovirus 1 OX=1195373 PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.36 GDD4 pKa = 3.67 EE5 pKa = 4.41 TPGRR9 pKa = 11.84 RR10 pKa = 11.84 WCFTINNYY18 pKa = 7.72 DD19 pKa = 3.88 TPDD22 pKa = 3.2 LTAVNEE28 pKa = 4.06 AFKK31 pKa = 11.03 EE32 pKa = 3.95 KK33 pKa = 10.69 DD34 pKa = 3.03 VVYY37 pKa = 9.99 AVCGKK42 pKa = 10.01 EE43 pKa = 3.91 VGKK46 pKa = 10.77 KK47 pKa = 7.54 GTKK50 pKa = 9.12 HH51 pKa = 5.1 LQGFIHH57 pKa = 6.42 FTGNWRR63 pKa = 11.84 FNRR66 pKa = 11.84 VKK68 pKa = 10.72 SLLGGRR74 pKa = 11.84 AHH76 pKa = 7.04 IEE78 pKa = 3.74 KK79 pKa = 10.69 ARR81 pKa = 11.84 GNDD84 pKa = 3.48 EE85 pKa = 4.02 QNKK88 pKa = 8.92 VYY90 pKa = 9.14 CTKK93 pKa = 10.73 EE94 pKa = 3.58 EE95 pKa = 4.34 TYY97 pKa = 11.11 LEE99 pKa = 4.28 VGSPQFQGKK108 pKa = 10.15 RR109 pKa = 11.84 NDD111 pKa = 3.29 LGRR114 pKa = 11.84 AVAALEE120 pKa = 4.72 GGSSLSEE127 pKa = 3.8 VAQANPEE134 pKa = 3.84 VFIRR138 pKa = 11.84 YY139 pKa = 8.7 GRR141 pKa = 11.84 GLRR144 pKa = 11.84 DD145 pKa = 3.38 YY146 pKa = 9.8 MNVRR150 pKa = 11.84 GLVKK154 pKa = 10.22 PRR156 pKa = 11.84 DD157 pKa = 3.98 FKK159 pKa = 9.91 THH161 pKa = 6.04 VIVLVGEE168 pKa = 4.32 PGSGKK173 pKa = 10.11 SKK175 pKa = 10.16 YY176 pKa = 9.83 ANEE179 pKa = 4.27 VEE181 pKa = 4.35 GTKK184 pKa = 9.81 YY185 pKa = 9.4 WKK187 pKa = 9.87 PRR189 pKa = 11.84 GQWWDD194 pKa = 3.5 GYY196 pKa = 10.31 NGEE199 pKa = 4.45 EE200 pKa = 4.84 VVVLDD205 pKa = 5.81 DD206 pKa = 4.04 FYY208 pKa = 11.81 GWVPYY213 pKa = 10.66 DD214 pKa = 3.32 EE215 pKa = 4.89 LLRR218 pKa = 11.84 VGDD221 pKa = 4.41 RR222 pKa = 11.84 YY223 pKa = 9.98 PLKK226 pKa = 10.61 VQVKK230 pKa = 8.45 GAFVDD235 pKa = 5.31 FVSKK239 pKa = 9.61 TLVITSNKK247 pKa = 9.45 RR248 pKa = 11.84 PEE250 pKa = 3.55 EE251 pKa = 4.22 WYY253 pKa = 10.19 DD254 pKa = 3.32 KK255 pKa = 11.21 EE256 pKa = 4.84 KK257 pKa = 10.93 IPDD260 pKa = 3.49 QSAMWRR266 pKa = 11.84 RR267 pKa = 11.84 FNEE270 pKa = 3.61 MYY272 pKa = 9.76 YY273 pKa = 10.17 CEE275 pKa = 4.48 RR276 pKa = 11.84 GEE278 pKa = 4.23 PIKK281 pKa = 10.21 PYY283 pKa = 9.16 PDD285 pKa = 2.78 EE286 pKa = 4.1 WKK288 pKa = 10.66 EE289 pKa = 3.76 FEE291 pKa = 4.56 TNFF294 pKa = 4.24
Molecular weight: 34.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.919
IPC2_protein 7.322
IPC_protein 7.366
Toseland 7.102
ProMoST 7.746
Dawson 8.039
Bjellqvist 8.156
Wikipedia 7.98
Rodwell 8.039
Grimsley 7.132
Solomon 8.17
Lehninger 8.17
Nozaki 8.287
DTASelect 8.068
Thurlkill 8.126
EMBOSS 8.258
Sillero 8.39
Patrickios 4.38
IPC_peptide 8.156
IPC2_peptide 7.161
IPC2.peptide.svr19 7.58
Protein with the highest isoelectric point:
>tr|I3VR57|I3VR57_9CIRC Cap OS=Rhinolophus ferrumequinum circovirus 1 OX=1195373 PE=3 SV=1
MM1 pKa = 7.38 PRR3 pKa = 11.84 STRR6 pKa = 11.84 HH7 pKa = 4.14 RR8 pKa = 11.84 WRR10 pKa = 11.84 RR11 pKa = 11.84 NQWFKK16 pKa = 10.53 RR17 pKa = 11.84 WRR19 pKa = 11.84 QRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 GHH26 pKa = 4.06 TRR28 pKa = 11.84 GRR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 YY33 pKa = 7.92 RR34 pKa = 11.84 NKK36 pKa = 9.55 VGIYY40 pKa = 9.0 NFRR43 pKa = 11.84 FRR45 pKa = 11.84 AITTMTINKK54 pKa = 9.37 NSNQGYY60 pKa = 7.57 FTYY63 pKa = 9.91 TLNGSVPTAFANYY76 pKa = 9.39 FDD78 pKa = 4.52 MYY80 pKa = 10.33 RR81 pKa = 11.84 IAKK84 pKa = 9.45 VRR86 pKa = 11.84 VQWLPMVSISEE97 pKa = 3.95 VRR99 pKa = 11.84 AWGATVIDD107 pKa = 3.75 LTGRR111 pKa = 11.84 DD112 pKa = 3.49 TTVPSTGRR120 pKa = 11.84 TDD122 pKa = 3.6 FTIDD126 pKa = 3.33 DD127 pKa = 3.77 STRR130 pKa = 11.84 RR131 pKa = 11.84 LWNPTRR137 pKa = 11.84 IHH139 pKa = 6.09 SRR141 pKa = 11.84 YY142 pKa = 7.42 FTPKK146 pKa = 9.66 PEE148 pKa = 3.6 IQIRR152 pKa = 11.84 SNSEE156 pKa = 3.39 AVQPNNPRR164 pKa = 11.84 NQLWIDD170 pKa = 3.55 SRR172 pKa = 11.84 DD173 pKa = 3.37 KK174 pKa = 10.96 DD175 pKa = 4.1 VKK177 pKa = 10.29 HH178 pKa = 6.6 HH179 pKa = 6.39 GIAYY183 pKa = 8.93 YY184 pKa = 8.25 FHH186 pKa = 7.83 PDD188 pKa = 3.84 DD189 pKa = 5.57 IGDD192 pKa = 3.8 DD193 pKa = 3.94 VYY195 pKa = 11.34 KK196 pKa = 10.57 FSYY199 pKa = 9.79 IVTYY203 pKa = 9.83 YY204 pKa = 10.36 FQFRR208 pKa = 11.84 QFAGSQAPSVV218 pKa = 3.58
Molecular weight: 26.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 10.16
IPC_protein 11.155
Toseland 11.038
ProMoST 11.272
Dawson 11.125
Bjellqvist 11.008
Wikipedia 11.491
Rodwell 11.052
Grimsley 11.184
Solomon 11.433
Lehninger 11.359
Nozaki 11.023
DTASelect 11.008
Thurlkill 11.052
EMBOSS 11.506
Sillero 11.082
Patrickios 10.716
IPC_peptide 11.433
IPC2_peptide 10.306
IPC2.peptide.svr19 8.431
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
512
218
294
256.0
30.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.492 ± 0.06
0.781 ± 0.497
5.859 ± 0.066
5.859 ± 2.853
5.273 ± 0.439
7.813 ± 1.76
1.953 ± 0.509
4.297 ± 1.352
6.641 ± 1.89
4.102 ± 1.15
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.563 ± 0.173
5.469 ± 0.315
4.883 ± 0.104
3.516 ± 0.682
9.961 ± 2.71
4.688 ± 1.104
6.25 ± 1.569
7.813 ± 1.177
3.125 ± 0.055
5.664 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here