Changjiang tombus-like virus 11
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFY8|A0A1L3KFY8_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 11 OX=1922804 PE=4 SV=1
MM1 pKa = 7.62 FKK3 pKa = 10.38 RR4 pKa = 11.84 AQTIRR9 pKa = 11.84 QYY11 pKa = 10.29 WSEE14 pKa = 3.85 FKK16 pKa = 10.99 KK17 pKa = 10.62 PVFVGLDD24 pKa = 3.14 ASRR27 pKa = 11.84 FDD29 pKa = 3.3 QHH31 pKa = 6.38 VSRR34 pKa = 11.84 EE35 pKa = 4.14 ALEE38 pKa = 4.47 FEE40 pKa = 4.5 HH41 pKa = 7.13 GLYY44 pKa = 10.08 TEE46 pKa = 4.38 VFRR49 pKa = 11.84 DD50 pKa = 3.45 PFLAYY55 pKa = 10.28 LLNMQIDD62 pKa = 3.95 QVGYY66 pKa = 11.17 ANMADD71 pKa = 3.52 GSVKK75 pKa = 10.76 YY76 pKa = 10.4 NVEE79 pKa = 3.86 GCRR82 pKa = 11.84 ASGDD86 pKa = 3.61 MNTALGNVFLMCVITYY102 pKa = 10.09 NYY104 pKa = 10.43 LHH106 pKa = 7.23 ALPCKK111 pKa = 9.37 WRR113 pKa = 11.84 FINDD117 pKa = 3.34 GDD119 pKa = 3.88 DD120 pKa = 3.34 CGIFIEE126 pKa = 4.66 HH127 pKa = 7.31 ADD129 pKa = 3.43 MHH131 pKa = 6.98 LLDD134 pKa = 5.19 EE135 pKa = 5.15 LPSHH139 pKa = 6.42 HH140 pKa = 6.88 LLYY143 pKa = 10.58 GFEE146 pKa = 4.57 MEE148 pKa = 5.09 VEE150 pKa = 4.09 DD151 pKa = 3.85 PVYY154 pKa = 10.26 IIEE157 pKa = 4.37 RR158 pKa = 11.84 VEE160 pKa = 4.34 FCQSRR165 pKa = 11.84 PVQLNEE171 pKa = 4.21 EE172 pKa = 4.04 EE173 pKa = 4.0 WMMVRR178 pKa = 11.84 NIHH181 pKa = 6.72 KK182 pKa = 10.15 AIKK185 pKa = 9.84 HH186 pKa = 5.56 DD187 pKa = 4.54 WICITSRR194 pKa = 11.84 DD195 pKa = 3.49 WATTEE200 pKa = 3.95 EE201 pKa = 4.25 NLVATSACGLALFGDD216 pKa = 4.46 VPVLGPMYY224 pKa = 9.98 QAMSRR229 pKa = 11.84 FPHH232 pKa = 6.04 RR233 pKa = 11.84 QRR235 pKa = 11.84 VVDD238 pKa = 4.75 KK239 pKa = 11.07 LLEE242 pKa = 4.36 TKK244 pKa = 10.37 EE245 pKa = 4.2 GWRR248 pKa = 11.84 SLLTGHH254 pKa = 7.4 RR255 pKa = 11.84 FNPVDD260 pKa = 3.48 STIARR265 pKa = 11.84 VSIYY269 pKa = 10.23 KK270 pKa = 10.44 AFGMLPDD277 pKa = 3.74 EE278 pKa = 4.24 QVEE281 pKa = 4.14 LEE283 pKa = 4.16 RR284 pKa = 11.84 EE285 pKa = 4.05 FRR287 pKa = 11.84 AFDD290 pKa = 3.57 PQNIKK295 pKa = 10.94 DD296 pKa = 3.67 KK297 pKa = 10.59 TSLYY301 pKa = 8.77 SQPSVRR307 pKa = 11.84 VQYY310 pKa = 10.75 IIDD313 pKa = 3.56 TT314 pKa = 4.1
Molecular weight: 36.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.131
IPC2_protein 5.181
IPC_protein 5.156
Toseland 5.232
ProMoST 5.347
Dawson 5.219
Bjellqvist 5.321
Wikipedia 5.118
Rodwell 5.156
Grimsley 5.207
Solomon 5.219
Lehninger 5.181
Nozaki 5.372
DTASelect 5.537
Thurlkill 5.283
EMBOSS 5.232
Sillero 5.448
Patrickios 3.795
IPC_peptide 5.232
IPC2_peptide 5.461
IPC2.peptide.svr19 5.377
Protein with the highest isoelectric point:
>tr|A0A1L3KFY8|A0A1L3KFY8_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 11 OX=1922804 PE=4 SV=1
MM1 pKa = 7.44 ALEE4 pKa = 4.93 IILLLVIAFEE14 pKa = 4.54 IIVRR18 pKa = 11.84 TKK20 pKa = 9.67 VWDD23 pKa = 3.63 AAGSLVTAALEE34 pKa = 4.52 GCGRR38 pKa = 11.84 AWTWMVPCFRR48 pKa = 11.84 AILPILRR55 pKa = 11.84 APILEE60 pKa = 4.33 VVSATSCWVSVLYY73 pKa = 10.61 HH74 pKa = 6.27 SLLMGHH80 pKa = 6.47 VRR82 pKa = 11.84 RR83 pKa = 11.84 QRR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 11.84 RR88 pKa = 11.84 IVLEE92 pKa = 3.76 SSKK95 pKa = 9.69 RR96 pKa = 11.84 TQRR99 pKa = 11.84 VLSVLDD105 pKa = 3.42 MDD107 pKa = 5.67 EE108 pKa = 5.21 IGDD111 pKa = 4.35 DD112 pKa = 3.63 EE113 pKa = 4.54 MEE115 pKa = 4.09 LVGRR119 pKa = 11.84 KK120 pKa = 9.63 LYY122 pKa = 10.09 VRR124 pKa = 11.84 CAVLISRR131 pKa = 11.84 RR132 pKa = 11.84 ARR134 pKa = 11.84 MGLKK138 pKa = 9.96 YY139 pKa = 10.25 PSYY142 pKa = 10.79 SPANEE147 pKa = 4.15 RR148 pKa = 11.84 IAADD152 pKa = 4.37 WILKK156 pKa = 9.25 HH157 pKa = 6.62 LPDD160 pKa = 5.01 DD161 pKa = 3.5 MTVGVRR167 pKa = 11.84 HH168 pKa = 5.96 KK169 pKa = 10.69 VLPLAVKK176 pKa = 8.37 LTFVRR181 pKa = 11.84 SHH183 pKa = 7.25 YY184 pKa = 9.61 EE185 pKa = 3.71 DD186 pKa = 3.56 KK187 pKa = 11.15 AALHH191 pKa = 5.33 FQGLKK196 pKa = 10.16 EE197 pKa = 3.9 YY198 pKa = 11.3 VDD200 pKa = 4.87 FSRR203 pKa = 11.84 HH204 pKa = 3.98
Molecular weight: 23.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.998
IPC2_protein 8.975
IPC_protein 9.063
Toseland 9.589
ProMoST 9.414
Dawson 9.882
Bjellqvist 9.648
Wikipedia 10.072
Rodwell 10.072
Grimsley 9.955
Solomon 9.94
Lehninger 9.897
Nozaki 9.765
DTASelect 9.589
Thurlkill 9.721
EMBOSS 10.028
Sillero 9.838
Patrickios 5.525
IPC_peptide 9.926
IPC2_peptide 8.58
IPC2.peptide.svr19 7.876
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
841
204
323
280.3
31.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.037 ± 1.396
1.784 ± 0.229
4.875 ± 0.837
4.875 ± 1.335
4.162 ± 0.759
6.421 ± 1.018
2.973 ± 0.322
4.4 ± 0.898
3.805 ± 0.198
9.512 ± 0.992
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.092 ± 0.384
4.043 ± 1.178
4.637 ± 0.508
3.448 ± 0.616
6.659 ± 1.135
5.945 ± 0.372
6.302 ± 1.554
8.442 ± 0.728
1.902 ± 0.178
3.686 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here