Howler monkey associated porprismacovirus 1
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5GP05|A0A0B5GP05_9VIRU Rep protein OS=Howler monkey associated porprismacovirus 1 OX=2170112 GN=rep PE=4 SV=1
MM1 pKa = 7.63 GLRR4 pKa = 11.84 RR5 pKa = 11.84 NIPTAPMLTALWPALLGISLKK26 pKa = 10.61 ARR28 pKa = 11.84 NIFTDD33 pKa = 4.49 FTSDD37 pKa = 3.17 NMATNRR43 pKa = 11.84 AYY45 pKa = 10.53 ASFQEE50 pKa = 4.64 IYY52 pKa = 10.64 DD53 pKa = 3.36 FHH55 pKa = 7.65 TEE57 pKa = 3.83 VGNTTLIEE65 pKa = 4.06 VHH67 pKa = 6.0 TPISDD72 pKa = 3.93 LPQRR76 pKa = 11.84 MLNGFFTQFRR86 pKa = 11.84 KK87 pKa = 9.94 FKK89 pKa = 10.6 YY90 pKa = 10.0 AGADD94 pKa = 3.34 VTLVPVSTLPADD106 pKa = 3.98 PLQISYY112 pKa = 10.45 EE113 pKa = 4.07 AGEE116 pKa = 4.09 PTIDD120 pKa = 3.8 PRR122 pKa = 11.84 DD123 pKa = 3.72 MVNPILWKK131 pKa = 10.26 GMSGASMGRR140 pKa = 11.84 FLDD143 pKa = 4.76 AIFGSHH149 pKa = 7.37 RR150 pKa = 11.84 IYY152 pKa = 10.7 SAQALGDD159 pKa = 3.75 VMDD162 pKa = 5.09 NSPASEE168 pKa = 3.85 TDD170 pKa = 4.25 FYY172 pKa = 11.67 QLFGDD177 pKa = 4.06 SLSISEE183 pKa = 4.29 STVFKK188 pKa = 11.07 ALTDD192 pKa = 4.05 PDD194 pKa = 3.61 AVAALEE200 pKa = 3.78 AMYY203 pKa = 10.7 YY204 pKa = 9.99 QGLADD209 pKa = 3.98 NSWNKK214 pKa = 10.71 AGVQTGFKK222 pKa = 10.3 KK223 pKa = 10.49 RR224 pKa = 11.84 GLFPLVWKK232 pKa = 10.23 SASNLFRR239 pKa = 11.84 PANQNSPPNPTGTGTPWVPATNVDD263 pKa = 3.51 DD264 pKa = 4.17 YY265 pKa = 12.07 NNYY268 pKa = 10.46 GYY270 pKa = 10.68 DD271 pKa = 3.53 QIHH274 pKa = 6.65 EE275 pKa = 4.57 SGSPLTPSVGVGIAGGQFSNGTTRR299 pKa = 11.84 LGWMPTQTSYY309 pKa = 11.91 GMVGTQPSIANAVNGAQDD327 pKa = 3.64 MTILPLIPMMMIILPPAYY345 pKa = 8.19 KK346 pKa = 9.62 TEE348 pKa = 3.83 MYY350 pKa = 10.12 FRR352 pKa = 11.84 MVIKK356 pKa = 10.44 HH357 pKa = 5.6 KK358 pKa = 11.14 FEE360 pKa = 4.13 FAEE363 pKa = 4.37 FANTVGPDD371 pKa = 3.32 TFSNNQNVSPAFIYY385 pKa = 10.78 TNVTSGASVATTTEE399 pKa = 4.15 EE400 pKa = 3.78 QPKK403 pKa = 10.16 IVTAGVAA410 pKa = 3.17
Molecular weight: 44.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.934
IPC2_protein 5.003
IPC_protein 4.94
Toseland 4.851
ProMoST 5.092
Dawson 4.94
Bjellqvist 5.067
Wikipedia 4.838
Rodwell 4.825
Grimsley 4.774
Solomon 4.94
Lehninger 4.889
Nozaki 5.054
DTASelect 5.258
Thurlkill 4.863
EMBOSS 4.876
Sillero 5.118
Patrickios 3.897
IPC_peptide 4.94
IPC2_peptide 5.105
IPC2.peptide.svr19 5.091
Protein with the highest isoelectric point:
>tr|A0A0B5GP05|A0A0B5GP05_9VIRU Rep protein OS=Howler monkey associated porprismacovirus 1 OX=2170112 GN=rep PE=4 SV=1
MM1 pKa = 7.14 STTASRR7 pKa = 11.84 QWSTKK12 pKa = 7.34 MSQMYY17 pKa = 9.38 MLTIPRR23 pKa = 11.84 DD24 pKa = 3.61 KK25 pKa = 10.75 VSKK28 pKa = 10.28 RR29 pKa = 11.84 EE30 pKa = 3.91 LRR32 pKa = 11.84 IMLDD36 pKa = 3.67 KK37 pKa = 11.05 NDD39 pKa = 3.76 CKK41 pKa = 10.81 KK42 pKa = 10.12 WIIGKK47 pKa = 7.57 EE48 pKa = 4.07 TGKK51 pKa = 10.3 NGYY54 pKa = 8.27 KK55 pKa = 9.59 HH56 pKa = 4.31 WQIRR60 pKa = 11.84 LEE62 pKa = 4.12 TSNEE66 pKa = 3.89 EE67 pKa = 3.92 FFDD70 pKa = 3.44 WCKK73 pKa = 10.98 KK74 pKa = 9.96 HH75 pKa = 6.73 IPTASIRR82 pKa = 11.84 KK83 pKa = 9.58 AEE85 pKa = 4.12 VPKK88 pKa = 9.91 WDD90 pKa = 3.73 YY91 pKa = 10.08 EE92 pKa = 4.29 AKK94 pKa = 10.29 EE95 pKa = 4.08 GQYY98 pKa = 7.49 WTSSDD103 pKa = 3.39 RR104 pKa = 11.84 TDD106 pKa = 3.91 NLIQRR111 pKa = 11.84 FGEE114 pKa = 3.8 FRR116 pKa = 11.84 PNQKK120 pKa = 10.02 RR121 pKa = 11.84 AIQALRR127 pKa = 11.84 ATNDD131 pKa = 3.24 RR132 pKa = 11.84 EE133 pKa = 4.13 VLVWYY138 pKa = 10.07 DD139 pKa = 3.09 EE140 pKa = 4.29 GGNVGKK146 pKa = 10.25 SWFTGALWEE155 pKa = 4.39 RR156 pKa = 11.84 GQAYY160 pKa = 7.24 VTPPTVSTVQAMVQFVASLYY180 pKa = 10.56 CAIEE184 pKa = 4.28 SIKK187 pKa = 11.02 DD188 pKa = 3.25 GLVYY192 pKa = 10.58 DD193 pKa = 3.63 SRR195 pKa = 11.84 YY196 pKa = 8.17 QGRR199 pKa = 11.84 MVNIRR204 pKa = 11.84 GVKK207 pKa = 9.51 IIVMTNNKK215 pKa = 9.0 PDD217 pKa = 4.21 LDD219 pKa = 3.88 KK220 pKa = 11.35 LSYY223 pKa = 10.57 DD224 pKa = 3.15 RR225 pKa = 11.84 WRR227 pKa = 11.84 MVVLL231 pKa = 4.26
Molecular weight: 27.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.896
IPC2_protein 8.916
IPC_protein 8.843
Toseland 9.56
ProMoST 9.311
Dawson 9.823
Bjellqvist 9.531
Wikipedia 9.999
Rodwell 10.145
Grimsley 9.897
Solomon 9.867
Lehninger 9.823
Nozaki 9.633
DTASelect 9.502
Thurlkill 9.663
EMBOSS 9.999
Sillero 9.765
Patrickios 5.308
IPC_peptide 9.853
IPC2_peptide 8.112
IPC2.peptide.svr19 7.93
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
641
231
410
320.5
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.8 ± 1.606
0.468 ± 0.512
5.46 ± 0.37
4.212 ± 0.872
4.524 ± 1.187
7.02 ± 0.858
1.092 ± 0.139
5.616 ± 0.274
5.148 ± 2.163
6.396 ± 0.474
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
4.056 ± 0.099
5.46 ± 0.697
5.928 ± 1.786
4.212 ± 0.339
4.836 ± 1.821
7.02 ± 0.325
8.112 ± 0.998
6.24 ± 0.423
2.496 ± 1.129
3.9 ± 0.264
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here