Aquimarina atlantica
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4786 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A023BS10|A0A023BS10_9FLAO Phosphatidate cytidylyltransferase OS=Aquimarina atlantica OX=1317122 GN=ATO12_21840 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.3 MAKK5 pKa = 9.81 GLPQILLLFSILVFKK20 pKa = 10.52 STFFYY25 pKa = 10.92 AQDD28 pKa = 3.23 FDD30 pKa = 4.35 VACRR34 pKa = 11.84 AAQPFCSDD42 pKa = 3.29 ASLEE46 pKa = 4.16 LTFPNVTGDD55 pKa = 3.23 MDD57 pKa = 4.08 GLGEE61 pKa = 4.23 VGCLNTTPNPAWYY74 pKa = 9.07 FIRR77 pKa = 11.84 IDD79 pKa = 3.58 QPGEE83 pKa = 4.02 LIFDD87 pKa = 3.96 IQQWVDD93 pKa = 3.51 SNDD96 pKa = 3.22 NSNLDD101 pKa = 3.39 RR102 pKa = 11.84 GEE104 pKa = 4.05 RR105 pKa = 11.84 QLDD108 pKa = 3.2 VDD110 pKa = 4.8 FIAWGPFPTSFVNCDD125 pKa = 3.47 ILSQGCDD132 pKa = 3.22 NNGDD136 pKa = 3.76 GDD138 pKa = 4.29 NIRR141 pKa = 11.84 PAEE144 pKa = 4.18 CVNNVDD150 pKa = 5.09 DD151 pKa = 4.84 PDD153 pKa = 4.4 YY154 pKa = 11.22 YY155 pKa = 10.9 VNNMDD160 pKa = 3.36 NTNIIDD166 pKa = 3.53 CSYY169 pKa = 11.53 ARR171 pKa = 11.84 TPDD174 pKa = 3.48 DD175 pKa = 4.86 DD176 pKa = 5.14 IYY178 pKa = 10.94 IEE180 pKa = 4.36 TLTIPNAQSGEE191 pKa = 4.17 YY192 pKa = 10.37 YY193 pKa = 10.19 IILITNFADD202 pKa = 3.61 EE203 pKa = 4.92 AGVIQLQQTNLNEE216 pKa = 4.62 DD217 pKa = 3.38 NAGTTDD223 pKa = 4.09 CSILEE228 pKa = 4.35 PGVGPDD234 pKa = 3.03 IATCGEE240 pKa = 4.07 FPITIEE246 pKa = 3.87 GRR248 pKa = 11.84 FPRR251 pKa = 11.84 AVTYY255 pKa = 9.38 QWARR259 pKa = 11.84 ADD261 pKa = 3.41 VGTTNFVNVPGPPGVIPFLEE281 pKa = 4.47 VTTEE285 pKa = 3.7 GVYY288 pKa = 8.85 QLRR291 pKa = 11.84 GFDD294 pKa = 3.37 EE295 pKa = 4.73 TGAEE299 pKa = 4.41 VPDD302 pKa = 4.25 SPDD305 pKa = 3.37 DD306 pKa = 3.99 LVVLDD311 pKa = 4.16 VSDD314 pKa = 4.14 AVVSVGYY321 pKa = 10.4 SIAEE325 pKa = 3.75 EE326 pKa = 4.39 SFAGGYY332 pKa = 8.09 TITAEE337 pKa = 4.24 VVAAPDD343 pKa = 3.38 VEE345 pKa = 4.53 AAGFDD350 pKa = 3.66 DD351 pKa = 5.22 FEE353 pKa = 4.63 YY354 pKa = 11.06 RR355 pKa = 11.84 LDD357 pKa = 3.83 RR358 pKa = 11.84 DD359 pKa = 3.11 IGNGFLEE366 pKa = 4.0 YY367 pKa = 10.54 RR368 pKa = 11.84 PFQSSPIFTNVPPGDD383 pKa = 3.72 YY384 pKa = 10.54 FITARR389 pKa = 11.84 YY390 pKa = 8.34 ANCPASEE397 pKa = 4.6 EE398 pKa = 4.02 ISDD401 pKa = 3.94 VIMILGYY408 pKa = 9.72 PKK410 pKa = 10.64 YY411 pKa = 9.13 FTPNGDD417 pKa = 3.65 GFHH420 pKa = 6.88 DD421 pKa = 3.78 TWSLINIEE429 pKa = 4.6 DD430 pKa = 3.67 QPTALIYY437 pKa = 10.63 IFDD440 pKa = 4.23 RR441 pKa = 11.84 YY442 pKa = 10.65 GKK444 pKa = 10.0 LLKK447 pKa = 9.8 QLRR450 pKa = 11.84 AGGPGWDD457 pKa = 3.18 GTYY460 pKa = 10.4 NGKK463 pKa = 9.74 NMPSSDD469 pKa = 2.8 YY470 pKa = 9.98 WFRR473 pKa = 11.84 VEE475 pKa = 4.92 FNEE478 pKa = 4.68 PRR480 pKa = 11.84 DD481 pKa = 3.44 PNMRR485 pKa = 11.84 RR486 pKa = 11.84 RR487 pKa = 11.84 VFAGNFSLIRR497 pKa = 4.27
Molecular weight: 55.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.696
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.63
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.783
Rodwell 3.681
Grimsley 3.541
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.795
Sillero 3.973
Patrickios 1.176
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.873
Protein with the highest isoelectric point:
>tr|A0A023BRY5|A0A023BRY5_9FLAO Uncharacterized protein OS=Aquimarina atlantica OX=1317122 GN=ATO12_21745 PE=4 SV=1
MM1 pKa = 7.3 ARR3 pKa = 11.84 IAGVDD8 pKa = 3.06 IPKK11 pKa = 10.08 QKK13 pKa = 10.42 RR14 pKa = 11.84 GVIALTYY21 pKa = 9.53 IFGIGRR27 pKa = 11.84 SRR29 pKa = 11.84 AKK31 pKa = 10.46 EE32 pKa = 3.61 ILEE35 pKa = 4.06 TAKK38 pKa = 10.17 VDD40 pKa = 3.69 EE41 pKa = 4.55 SVKK44 pKa = 10.68 VSDD47 pKa = 4.68 WDD49 pKa = 3.64 DD50 pKa = 3.74 DD51 pKa = 4.05 QIGRR55 pKa = 11.84 IRR57 pKa = 11.84 EE58 pKa = 3.97 AVGAYY63 pKa = 8.96 TIEE66 pKa = 4.71 GEE68 pKa = 4.31 LRR70 pKa = 11.84 SEE72 pKa = 3.94 VQLNIKK78 pKa = 10.12 RR79 pKa = 11.84 LMDD82 pKa = 3.77 IGCYY86 pKa = 9.51 RR87 pKa = 11.84 GIRR90 pKa = 11.84 HH91 pKa = 6.49 RR92 pKa = 11.84 AGLPLRR98 pKa = 11.84 GQRR101 pKa = 11.84 TKK103 pKa = 11.3 NNSRR107 pKa = 11.84 TRR109 pKa = 11.84 KK110 pKa = 9.17 GRR112 pKa = 11.84 RR113 pKa = 11.84 KK114 pKa = 7.81 TVANKK119 pKa = 10.07 KK120 pKa = 9.89 KK121 pKa = 9.38 ATKK124 pKa = 10.14
Molecular weight: 14.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.736
IPC_protein 10.452
Toseland 10.935
ProMoST 10.643
Dawson 10.994
Bjellqvist 10.687
Wikipedia 11.199
Rodwell 11.272
Grimsley 11.023
Solomon 11.14
Lehninger 11.111
Nozaki 10.906
DTASelect 10.687
Thurlkill 10.921
EMBOSS 11.33
Sillero 10.921
Patrickios 10.994
IPC_peptide 11.155
IPC2_peptide 9.458
IPC2.peptide.svr19 8.739
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4786
0
4786
1642244
59
7139
343.1
38.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.875 ± 0.036
0.77 ± 0.013
5.587 ± 0.026
6.526 ± 0.041
5.154 ± 0.031
6.266 ± 0.04
1.828 ± 0.018
8.533 ± 0.036
7.976 ± 0.048
9.142 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.024 ± 0.015
6.178 ± 0.042
3.314 ± 0.023
3.41 ± 0.023
3.399 ± 0.023
6.656 ± 0.031
6.06 ± 0.044
5.955 ± 0.026
1.068 ± 0.013
4.281 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here