Avian orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0QNN6|A0A0A0QNN6_9REOV Lambda-A protein OS=Avian orthoreovirus OX=38170 PE=4 SV=1
MM1 pKa = 7.65AGLNPSQRR9 pKa = 11.84RR10 pKa = 11.84EE11 pKa = 4.05VVSLILSLTSNVTISHH27 pKa = 6.87GDD29 pKa = 3.15LTPIYY34 pKa = 10.28EE35 pKa = 4.23RR36 pKa = 11.84LTNLEE41 pKa = 4.02ASTEE45 pKa = 3.98LLHH48 pKa = 7.69RR49 pKa = 11.84SISDD53 pKa = 2.8ISTTVSNISANLQDD67 pKa = 3.83MTHH70 pKa = 7.01ILDD73 pKa = 4.86DD74 pKa = 3.6VTANLDD80 pKa = 3.68GLRR83 pKa = 11.84TTVTALQDD91 pKa = 3.64FVSILSTNVTDD102 pKa = 4.29LTNTSSAHH110 pKa = 6.06AATLSLLQTTVDD122 pKa = 3.85GNSTAISNLKK132 pKa = 10.39SDD134 pKa = 3.63VSSNGLAITDD144 pKa = 3.58LQDD147 pKa = 3.09RR148 pKa = 11.84VKK150 pKa = 10.73SLEE153 pKa = 3.99STASHH158 pKa = 6.46GLSFSPPLSVADD170 pKa = 3.95GVVSLDD176 pKa = 3.35MDD178 pKa = 4.77PYY180 pKa = 10.25FCSQRR185 pKa = 11.84VSLTSYY191 pKa = 10.23SAEE194 pKa = 3.78AQLMQFRR201 pKa = 11.84WMARR205 pKa = 11.84GTNGSSDD212 pKa = 4.26TIDD215 pKa = 3.26MTVNAHH221 pKa = 4.55CHH223 pKa = 4.55GRR225 pKa = 11.84RR226 pKa = 11.84TDD228 pKa = 3.5YY229 pKa = 11.04MMSSTGNLTVTSNVVLLTFDD249 pKa = 4.46LSDD252 pKa = 3.36ITHH255 pKa = 6.82IPSDD259 pKa = 3.94LARR262 pKa = 11.84LVPSAGFQAASFPVDD277 pKa = 3.17VSFTRR282 pKa = 11.84DD283 pKa = 3.46SATHH287 pKa = 7.2AYY289 pKa = 8.45QAYY292 pKa = 9.19GVYY295 pKa = 10.18SSSRR299 pKa = 11.84VFTITFPTGGDD310 pKa = 3.06GTANIRR316 pKa = 11.84SLTVRR321 pKa = 11.84TGIDD325 pKa = 3.04TT326 pKa = 3.86

Molecular weight: 34.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O12286|O12286_9REOV 17 kDa protein OS=Avian orthoreovirus OX=38170 PE=4 SV=1
MM1 pKa = 7.61LRR3 pKa = 11.84MPPGSCNGATAVFGNVHH20 pKa = 6.01CQAAQNTAGGDD31 pKa = 3.67LQATSSIIAYY41 pKa = 8.46WPYY44 pKa = 10.5LAAGGGFLLIVIIFALLYY62 pKa = 9.68CCKK65 pKa = 10.66AKK67 pKa = 10.82VKK69 pKa = 10.62ADD71 pKa = 3.44AARR74 pKa = 11.84SVFHH78 pKa = 7.18RR79 pKa = 11.84EE80 pKa = 3.58LVALSSGKK88 pKa = 9.54HH89 pKa = 4.76NAMAPPYY96 pKa = 10.3DD97 pKa = 3.6VV98 pKa = 4.1

Molecular weight: 10.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

7600

98

1293

633.3

69.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.395 ± 0.466

1.632 ± 0.138

6.145 ± 0.226

3.408 ± 0.363

4.0 ± 0.327

5.0 ± 0.262

2.039 ± 0.194

4.803 ± 0.212

2.711 ± 0.27

10.276 ± 0.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.231

4.013 ± 0.401

6.145 ± 0.373

3.961 ± 0.351

5.987 ± 0.331

9.829 ± 0.416

7.197 ± 0.598

7.289 ± 0.264

1.566 ± 0.159

3.026 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski