Duck adenovirus 2
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A075FAC0|A0A075FAC0_9ADEN ORF55A OS=Duck adenovirus 2 OX=1520006 PE=4 SV=1
MM1 pKa = 7.77 AEE3 pKa = 4.61 GGQDD7 pKa = 3.59 PNQPDD12 pKa = 3.57 PMRR15 pKa = 11.84 EE16 pKa = 3.78 LEE18 pKa = 4.4 RR19 pKa = 11.84 IEE21 pKa = 4.24 EE22 pKa = 3.94 EE23 pKa = 4.48 LMRR26 pKa = 11.84 IDD28 pKa = 5.87 DD29 pKa = 4.0 EE30 pKa = 6.44 RR31 pKa = 11.84 IMNNRR36 pKa = 11.84 ALADD40 pKa = 3.1 IRR42 pKa = 11.84 ALVDD46 pKa = 4.19 EE47 pKa = 5.56 IEE49 pKa = 5.6 NEE51 pKa = 4.01 MQQNEE56 pKa = 4.22 NQEE59 pKa = 4.14 GEE61 pKa = 4.19 EE62 pKa = 4.49 DD63 pKa = 3.99 DD64 pKa = 4.34 LLNYY68 pKa = 10.33 DD69 pKa = 4.25 VLQLMSFEE77 pKa = 4.0 NHH79 pKa = 6.45 HH80 pKa = 6.95 IIVSSCQCHH89 pKa = 4.54 NWYY92 pKa = 8.74 MRR94 pKa = 11.84 DD95 pKa = 3.05 FCMSIFSNLEE105 pKa = 3.69 SPIFPTVWSSLADD118 pKa = 3.99 DD119 pKa = 4.86 PDD121 pKa = 5.1 LYY123 pKa = 11.4 GCYY126 pKa = 10.04 FISSNMAAMFSRR138 pKa = 11.84 MDD140 pKa = 3.67 PVFMDD145 pKa = 3.2 RR146 pKa = 11.84 RR147 pKa = 11.84 VGHH150 pKa = 4.99 SHH152 pKa = 6.03 GVIINVSEE160 pKa = 3.95 LTRR163 pKa = 11.84 YY164 pKa = 7.22 TFLSAVCAIADD175 pKa = 3.88 MGAMPCEE182 pKa = 4.57 YY183 pKa = 10.01 IRR185 pKa = 11.84 FMSLMDD191 pKa = 3.95 INQLFLSCPRR201 pKa = 11.84 WMFEE205 pKa = 4.38 LMQIFTKK212 pKa = 10.57 YY213 pKa = 9.31 YY214 pKa = 8.16 SEE216 pKa = 5.67 RR217 pKa = 11.84 MMTMM221 pKa = 3.48
Molecular weight: 25.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.094
IPC2_protein 4.291
IPC_protein 4.24
Toseland 4.062
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.075
Rodwell 4.075
Grimsley 3.973
Solomon 4.19
Lehninger 4.139
Nozaki 4.304
DTASelect 4.469
Thurlkill 4.075
EMBOSS 4.088
Sillero 4.355
Patrickios 2.651
IPC_peptide 4.19
IPC2_peptide 4.342
IPC2.peptide.svr19 4.261
Protein with the highest isoelectric point:
>tr|A0A075FA61|A0A075FA61_9ADEN DBP OS=Duck adenovirus 2 OX=1520006 PE=4 SV=1
MM1 pKa = 7.74 SILISPSDD9 pKa = 3.59 NRR11 pKa = 11.84 GWGAMMRR18 pKa = 11.84 RR19 pKa = 11.84 SRR21 pKa = 11.84 SRR23 pKa = 11.84 SVMRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 PSMRR33 pKa = 11.84 GTGLRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 LTRR43 pKa = 11.84 VVRR46 pKa = 11.84 AVLPAVPGPVRR57 pKa = 11.84 SLLGFGRR64 pKa = 11.84 RR65 pKa = 11.84 RR66 pKa = 11.84 MRR68 pKa = 11.84 RR69 pKa = 11.84 SGSRR73 pKa = 11.84 TTLVAVTSTRR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 RR86 pKa = 11.84 RR87 pKa = 11.84 RR88 pKa = 3.19
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
0
36
13216
40
1341
367.1
41.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.114 ± 0.321
1.907 ± 0.233
5.66 ± 0.287
5.561 ± 0.58
4.404 ± 0.179
5.751 ± 0.283
2.467 ± 0.216
5.607 ± 0.248
4.918 ± 0.392
8.074 ± 0.405
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.057 ± 0.234
5.281 ± 0.494
5.985 ± 0.382
3.352 ± 0.244
5.524 ± 0.499
8.058 ± 0.405
6.008 ± 0.364
6.78 ± 0.388
1.271 ± 0.086
4.222 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here