Parapedobacter luteus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4124 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T5DU17|A0A1T5DU17_9SPHI 3-oxoacyl-(Acyl-carrier-protein) synthase OS=Parapedobacter luteus OX=623280 GN=SAMN05660226_02982 PE=4 SV=1
MM1 pKa = 8.13 SEE3 pKa = 4.01 FCDD6 pKa = 4.19 VNDD9 pKa = 5.57 LGDD12 pKa = 3.94 FLIKK16 pKa = 10.43 EE17 pKa = 4.01 MGMYY21 pKa = 9.71 IDD23 pKa = 3.99 SDD25 pKa = 3.79 LRR27 pKa = 11.84 NSYY30 pKa = 10.93 LNAYY34 pKa = 9.36 VLTSNFKK41 pKa = 10.38 NAII44 pKa = 3.74
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 4.101
IPC_protein 3.91
Toseland 3.706
ProMoST 4.113
Dawson 3.923
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.63
Solomon 3.884
Lehninger 3.846
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.037
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|A0A1T5F9K7|A0A1T5F9K7_9SPHI Cytochrome c mono-and diheme variants OS=Parapedobacter luteus OX=623280 GN=SAMN05660226_03841 PE=4 SV=1
MM1 pKa = 7.62 RR2 pKa = 11.84 PSTVVRR8 pKa = 11.84 ATCTMRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 ILQEE20 pKa = 3.59 PEE22 pKa = 3.63 AQYY25 pKa = 9.26 KK26 pKa = 7.97 TIRR29 pKa = 11.84 IDD31 pKa = 3.35 TEE33 pKa = 3.5 EE34 pKa = 4.1 DD35 pKa = 3.65 VFVRR39 pKa = 11.84 NRR41 pKa = 11.84 LFNRR45 pKa = 11.84 HH46 pKa = 5.72 ILRR49 pKa = 11.84 LYY51 pKa = 10.58 QSTCSFTGMRR61 pKa = 11.84 LVSRR65 pKa = 11.84 HH66 pKa = 4.45 GHH68 pKa = 5.07 SFVDD72 pKa = 4.38 GFWKK76 pKa = 10.38 ILSKK80 pKa = 10.56 FVNCGLYY87 pKa = 10.14 QSNHH91 pKa = 4.36 YY92 pKa = 9.74 NRR94 pKa = 11.84 NKK96 pKa = 10.49 SS97 pKa = 3.15
Molecular weight: 11.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.706
IPC_protein 10.599
Toseland 10.496
ProMoST 10.277
Dawson 10.643
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.774
Grimsley 10.716
Solomon 10.76
Lehninger 10.716
Nozaki 10.526
DTASelect 10.394
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.496
IPC_peptide 10.76
IPC2_peptide 9.677
IPC2.peptide.svr19 8.412
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4124
0
4124
1476030
12
3151
357.9
40.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.51 ± 0.039
0.75 ± 0.012
5.59 ± 0.026
5.74 ± 0.036
4.648 ± 0.028
7.354 ± 0.039
2.194 ± 0.02
6.474 ± 0.03
5.05 ± 0.037
9.657 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.262 ± 0.016
4.735 ± 0.04
4.135 ± 0.022
3.87 ± 0.022
5.361 ± 0.029
5.876 ± 0.028
5.611 ± 0.035
6.76 ± 0.036
1.322 ± 0.014
4.1 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here