Zostera marina amalgavirus 2
Average proteome isoelectric point is 8.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6R5F1|A0A1W6R5F1_9VIRU Fusion protein OS=Zostera marina amalgavirus 2 OX=1985203 PE=4 SV=1
MM1 pKa = 7.64 FFRR4 pKa = 11.84 CEE6 pKa = 3.51 KK7 pKa = 9.48 MANEE11 pKa = 5.15 DD12 pKa = 3.74 GSSVPLKK19 pKa = 9.6 TQAQVDD25 pKa = 4.13 EE26 pKa = 4.66 EE27 pKa = 4.8 RR28 pKa = 11.84 DD29 pKa = 3.57 VAQLNKK35 pKa = 10.25 ALSYY39 pKa = 11.09 LDD41 pKa = 3.94 LGGMPIAPWALQDD54 pKa = 4.62 VADD57 pKa = 4.36 CSYY60 pKa = 10.67 TVARR64 pKa = 11.84 ALRR67 pKa = 11.84 KK68 pKa = 9.82 LKK70 pKa = 10.11 ILKK73 pKa = 8.86 PHH75 pKa = 6.9 YY76 pKa = 10.01 DD77 pKa = 3.41 NKK79 pKa = 10.5 HH80 pKa = 4.56 LQKK83 pKa = 10.62 LFHH86 pKa = 6.25 YY87 pKa = 9.78 CEE89 pKa = 4.11 EE90 pKa = 4.12 NAVIDD95 pKa = 3.65 STLPLKK101 pKa = 10.67 LSSVFRR107 pKa = 11.84 FCDD110 pKa = 3.14 WLLSPVAKK118 pKa = 10.23 RR119 pKa = 11.84 KK120 pKa = 9.61 IEE122 pKa = 3.86 QLVNADD128 pKa = 3.92 RR129 pKa = 11.84 LKK131 pKa = 10.82 KK132 pKa = 10.14 RR133 pKa = 11.84 SRR135 pKa = 11.84 DD136 pKa = 3.51 AITPAEE142 pKa = 4.09 TALVAILEE150 pKa = 4.43 VAQNDD155 pKa = 3.87 CVADD159 pKa = 3.58 VSRR162 pKa = 11.84 VRR164 pKa = 11.84 ITYY167 pKa = 10.24 DD168 pKa = 3.34 EE169 pKa = 4.69 EE170 pKa = 3.99 IKK172 pKa = 10.67 KK173 pKa = 10.48 LKK175 pKa = 10.47 RR176 pKa = 11.84 KK177 pKa = 9.17 IGKK180 pKa = 9.57 LEE182 pKa = 3.57 EE183 pKa = 3.94 HH184 pKa = 7.18 KK185 pKa = 10.9 ARR187 pKa = 11.84 KK188 pKa = 8.86 IEE190 pKa = 4.05 KK191 pKa = 9.74 ARR193 pKa = 11.84 KK194 pKa = 8.97 KK195 pKa = 10.92 YY196 pKa = 10.14 PGLLLLEE203 pKa = 4.89 RR204 pKa = 11.84 PSEE207 pKa = 4.36 SDD209 pKa = 3.21 VCNQAWHH216 pKa = 6.91 KK217 pKa = 10.12 YY218 pKa = 8.54 VEE220 pKa = 4.3 YY221 pKa = 10.76 CNASGIKK228 pKa = 8.37 QEE230 pKa = 4.09 KK231 pKa = 9.62 RR232 pKa = 11.84 SNASLEE238 pKa = 4.17 KK239 pKa = 10.23 AISMFDD245 pKa = 3.18 NILRR249 pKa = 11.84 LEE251 pKa = 4.31 IKK253 pKa = 10.19 AKK255 pKa = 10.19 CCEE258 pKa = 4.0 KK259 pKa = 11.0 PEE261 pKa = 3.95 VRR263 pKa = 11.84 DD264 pKa = 4.32 YY265 pKa = 11.63 LLQYY269 pKa = 10.37 CKK271 pKa = 10.75 EE272 pKa = 4.15 KK273 pKa = 10.79 IKK275 pKa = 10.76 GFRR278 pKa = 11.84 DD279 pKa = 3.39 DD280 pKa = 4.07 ADD282 pKa = 3.62 KK283 pKa = 11.44 KK284 pKa = 10.97 RR285 pKa = 11.84 VDD287 pKa = 3.59 TFKK290 pKa = 10.9 GYY292 pKa = 10.75 YY293 pKa = 9.17 SAATGEE299 pKa = 4.25 PLPEE303 pKa = 4.51 GSSRR307 pKa = 11.84 KK308 pKa = 9.42 KK309 pKa = 10.39 RR310 pKa = 11.84 RR311 pKa = 11.84 TAASDD316 pKa = 3.3 SSGDD320 pKa = 3.9 SEE322 pKa = 5.41 SDD324 pKa = 3.43 GASEE328 pKa = 4.73 RR329 pKa = 11.84 DD330 pKa = 3.63 DD331 pKa = 4.2 VVAGDD336 pKa = 3.6 FVDD339 pKa = 6.21 RR340 pKa = 11.84 IFKK343 pKa = 9.75 EE344 pKa = 3.99 QTSGGEE350 pKa = 4.06 QSEE353 pKa = 4.63 TPPPQEE359 pKa = 4.24 TRR361 pKa = 11.84 DD362 pKa = 3.34 ADD364 pKa = 3.5 IADD367 pKa = 4.02 EE368 pKa = 4.25 SSHH371 pKa = 6.04 GKK373 pKa = 10.05 SKK375 pKa = 10.75 GKK377 pKa = 9.63 SASRR381 pKa = 11.84 LRR383 pKa = 11.84 SRR385 pKa = 11.84 IRR387 pKa = 11.84 TRR389 pKa = 11.84 AQNRR393 pKa = 11.84 KK394 pKa = 9.01 KK395 pKa = 10.81
Molecular weight: 44.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.415
IPC2_protein 8.302
IPC_protein 8.156
Toseland 8.799
ProMoST 8.814
Dawson 9.165
Bjellqvist 9.107
Wikipedia 9.253
Rodwell 9.414
Grimsley 9.033
Solomon 9.238
Lehninger 9.224
Nozaki 9.341
DTASelect 8.96
Thurlkill 9.121
EMBOSS 9.355
Sillero 9.311
Patrickios 4.991
IPC_peptide 9.238
IPC2_peptide 7.746
IPC2.peptide.svr19 7.72
Protein with the highest isoelectric point:
>tr|A0A1W6R5F1|A0A1W6R5F1_9VIRU Fusion protein OS=Zostera marina amalgavirus 2 OX=1985203 PE=4 SV=1
MM1 pKa = 7.64 FFRR4 pKa = 11.84 CEE6 pKa = 3.51 KK7 pKa = 9.48 MANEE11 pKa = 5.15 DD12 pKa = 3.74 GSSVPLKK19 pKa = 9.6 TQAQVDD25 pKa = 4.13 EE26 pKa = 4.66 EE27 pKa = 4.8 RR28 pKa = 11.84 DD29 pKa = 3.57 VAQLNKK35 pKa = 10.25 ALSYY39 pKa = 11.09 LDD41 pKa = 3.94 LGGMPIAPWALQDD54 pKa = 4.62 VADD57 pKa = 4.36 CSYY60 pKa = 10.67 TVARR64 pKa = 11.84 ALRR67 pKa = 11.84 KK68 pKa = 9.82 LKK70 pKa = 10.11 ILKK73 pKa = 8.86 PHH75 pKa = 6.9 YY76 pKa = 10.01 DD77 pKa = 3.41 NKK79 pKa = 10.5 HH80 pKa = 4.56 LQKK83 pKa = 10.62 LFHH86 pKa = 6.25 YY87 pKa = 9.78 CEE89 pKa = 4.11 EE90 pKa = 4.12 NAVIDD95 pKa = 3.65 STLPLKK101 pKa = 10.67 LSSVFRR107 pKa = 11.84 FCDD110 pKa = 3.14 WLLSPVAKK118 pKa = 10.23 RR119 pKa = 11.84 KK120 pKa = 9.61 IEE122 pKa = 3.86 QLVNADD128 pKa = 3.92 RR129 pKa = 11.84 LKK131 pKa = 10.82 KK132 pKa = 10.14 RR133 pKa = 11.84 SRR135 pKa = 11.84 DD136 pKa = 3.51 AITPAEE142 pKa = 4.09 TALVAILEE150 pKa = 4.43 VAQNDD155 pKa = 3.87 CVADD159 pKa = 3.58 VSRR162 pKa = 11.84 VRR164 pKa = 11.84 ITYY167 pKa = 10.24 DD168 pKa = 3.34 EE169 pKa = 4.69 EE170 pKa = 3.99 IKK172 pKa = 10.67 KK173 pKa = 10.48 LKK175 pKa = 10.47 RR176 pKa = 11.84 KK177 pKa = 9.17 IGKK180 pKa = 9.57 LEE182 pKa = 3.57 EE183 pKa = 3.94 HH184 pKa = 7.18 KK185 pKa = 10.9 ARR187 pKa = 11.84 KK188 pKa = 8.86 IEE190 pKa = 4.05 KK191 pKa = 9.74 ARR193 pKa = 11.84 KK194 pKa = 8.97 KK195 pKa = 10.92 YY196 pKa = 10.14 PGLLLLEE203 pKa = 4.89 RR204 pKa = 11.84 PSEE207 pKa = 4.36 SDD209 pKa = 3.21 VCNQAWHH216 pKa = 6.91 KK217 pKa = 10.12 YY218 pKa = 8.54 VEE220 pKa = 4.3 YY221 pKa = 10.76 CNASGIKK228 pKa = 8.37 QEE230 pKa = 4.09 KK231 pKa = 9.62 RR232 pKa = 11.84 SNASLEE238 pKa = 4.17 KK239 pKa = 10.23 AISMFDD245 pKa = 3.18 NILRR249 pKa = 11.84 LEE251 pKa = 4.31 IKK253 pKa = 10.19 AKK255 pKa = 10.19 CCEE258 pKa = 4.0 KK259 pKa = 11.0 PEE261 pKa = 3.95 VRR263 pKa = 11.84 DD264 pKa = 4.32 YY265 pKa = 11.63 LLQYY269 pKa = 10.37 CKK271 pKa = 10.67 EE272 pKa = 4.13 KK273 pKa = 10.72 IKK275 pKa = 11.08 GFVTTPIKK283 pKa = 10.64 SALTHH288 pKa = 6.12 SRR290 pKa = 11.84 DD291 pKa = 3.59 TILQRR296 pKa = 11.84 LEE298 pKa = 3.95 SHH300 pKa = 6.56 FLKK303 pKa = 10.88 APLEE307 pKa = 4.1 RR308 pKa = 11.84 RR309 pKa = 11.84 EE310 pKa = 4.16 EE311 pKa = 4.24 LLPLIPVGTVNPMGHH326 pKa = 7.16 LNATMSLRR334 pKa = 11.84 EE335 pKa = 4.02 ILWTEE340 pKa = 3.83 SLKK343 pKa = 10.61 NRR345 pKa = 11.84 QVEE348 pKa = 4.32 GSKK351 pKa = 10.53 VKK353 pKa = 10.51 PPLPRR358 pKa = 11.84 KK359 pKa = 10.15 LEE361 pKa = 3.92 MLILPMNLLMANPRR375 pKa = 11.84 VNLLHH380 pKa = 6.83 AFVHH384 pKa = 6.29 ASGRR388 pKa = 11.84 GLKK391 pKa = 9.5 IARR394 pKa = 11.84 SKK396 pKa = 10.29 WEE398 pKa = 3.87 AGVRR402 pKa = 11.84 RR403 pKa = 11.84 IVGGGEE409 pKa = 3.54 MRR411 pKa = 11.84 GWNEE415 pKa = 3.6 DD416 pKa = 3.24 SAKK419 pKa = 10.31 YY420 pKa = 10.1 RR421 pKa = 11.84 GGGNLHH427 pKa = 6.68 DD428 pKa = 5.95 AIRR431 pKa = 11.84 VLATGRR437 pKa = 11.84 HH438 pKa = 5.85 DD439 pKa = 3.98 LPGSFLFQHH448 pKa = 6.31 FNFRR452 pKa = 11.84 SARR455 pKa = 11.84 SILKK459 pKa = 10.23 LPSDD463 pKa = 4.24 LSVPDD468 pKa = 3.85 GRR470 pKa = 11.84 DD471 pKa = 3.05 SVTMRR476 pKa = 11.84 NFNEE480 pKa = 4.09 EE481 pKa = 3.71 ATAGPVLRR489 pKa = 11.84 AIGCKK494 pKa = 8.6 TKK496 pKa = 10.83 YY497 pKa = 9.63 GLKK500 pKa = 10.22 RR501 pKa = 11.84 GLEE504 pKa = 4.27 DD505 pKa = 4.48 IVWNLYY511 pKa = 10.66 DD512 pKa = 3.38 RR513 pKa = 11.84 VGNGEE518 pKa = 4.08 LRR520 pKa = 11.84 TWEE523 pKa = 4.65 LPPLLARR530 pKa = 11.84 IGFRR534 pKa = 11.84 SKK536 pKa = 11.14 LIDD539 pKa = 3.35 QDD541 pKa = 3.55 KK542 pKa = 11.27 AEE544 pKa = 4.27 EE545 pKa = 4.8 KK546 pKa = 10.49 ILTGQPLGRR555 pKa = 11.84 AVMMLDD561 pKa = 3.25 ASEE564 pKa = 4.27 QAFSSPLYY572 pKa = 9.67 NVLSGVVSRR581 pKa = 11.84 LNCEE585 pKa = 4.04 RR586 pKa = 11.84 RR587 pKa = 11.84 SGWRR591 pKa = 11.84 NAVVRR596 pKa = 11.84 ASSDD600 pKa = 2.42 WSRR603 pKa = 11.84 FWKK606 pKa = 10.37 DD607 pKa = 2.81 IRR609 pKa = 11.84 DD610 pKa = 3.7 AKK612 pKa = 10.87 CIVEE616 pKa = 4.34 LDD618 pKa = 3.0 WSKK621 pKa = 10.98 FDD623 pKa = 4.28 RR624 pKa = 11.84 EE625 pKa = 4.05 RR626 pKa = 11.84 PRR628 pKa = 11.84 ADD630 pKa = 2.79 IQFCIDD636 pKa = 3.55 VLCSCFRR643 pKa = 11.84 PRR645 pKa = 11.84 GKK647 pKa = 9.94 RR648 pKa = 11.84 QRR650 pKa = 11.84 RR651 pKa = 11.84 LLSAYY656 pKa = 9.81 KK657 pKa = 10.76 YY658 pKa = 8.7 MLEE661 pKa = 3.75 NALIHH666 pKa = 5.96 KK667 pKa = 10.04 VIMLDD672 pKa = 3.44 DD673 pKa = 4.21 GCAFEE678 pKa = 4.5 YY679 pKa = 10.76 EE680 pKa = 4.3 GMIPSGSLWTGILGTAMNILYY701 pKa = 8.42 ITAACEE707 pKa = 3.93 SCGVKK712 pKa = 10.18 RR713 pKa = 11.84 EE714 pKa = 4.21 TFVPFCAGDD723 pKa = 3.8 DD724 pKa = 3.76 NLTVFDD730 pKa = 4.19 SAVDD734 pKa = 3.36 EE735 pKa = 4.66 GMLLNIRR742 pKa = 11.84 VFLNNMFRR750 pKa = 11.84 AGIEE754 pKa = 3.99 EE755 pKa = 4.37 KK756 pKa = 10.69 DD757 pKa = 3.88 FLIHH761 pKa = 5.79 YY762 pKa = 7.48 PPYY765 pKa = 10.54 HH766 pKa = 4.86 VTKK769 pKa = 10.02 VQAVFPDD776 pKa = 4.33 DD777 pKa = 4.24 FDD779 pKa = 6.25 LSLGTSKK786 pKa = 11.22 YY787 pKa = 10.37 LDD789 pKa = 3.32 KK790 pKa = 11.16 CVWVPFEE797 pKa = 4.73 GPLLVDD803 pKa = 3.31 TSRR806 pKa = 11.84 GYY808 pKa = 7.59 SHH810 pKa = 7.13 RR811 pKa = 11.84 WNYY814 pKa = 8.59 VFKK817 pKa = 10.94 GKK819 pKa = 9.64 PKK821 pKa = 10.15 FLANYY826 pKa = 8.79 FLEE829 pKa = 5.02 DD830 pKa = 3.3 GRR832 pKa = 11.84 SIRR835 pKa = 11.84 PAHH838 pKa = 6.98 DD839 pKa = 3.45 NMEE842 pKa = 4.21 KK843 pKa = 10.83 LLFPEE848 pKa = 5.24 NIQEE852 pKa = 4.49 HH853 pKa = 6.18 IEE855 pKa = 4.27 DD856 pKa = 3.98 YY857 pKa = 10.64 EE858 pKa = 4.03 AAILSMVVDD867 pKa = 4.46 NPWNSHH873 pKa = 4.52 NVNHH877 pKa = 6.73 LMQRR881 pKa = 11.84 YY882 pKa = 9.62 VIVQQIKK889 pKa = 9.39 RR890 pKa = 11.84 QSIPPLNAAEE900 pKa = 4.34 VLFCSKK906 pKa = 10.03 FRR908 pKa = 11.84 EE909 pKa = 4.4 IADD912 pKa = 3.51 NMYY915 pKa = 10.7 LFPTVGYY922 pKa = 9.08 WRR924 pKa = 11.84 RR925 pKa = 11.84 QLPGVRR931 pKa = 11.84 MEE933 pKa = 5.39 SIPEE937 pKa = 3.66 LRR939 pKa = 11.84 PIVEE943 pKa = 4.3 EE944 pKa = 4.18 FSQFVSGVTSLYY956 pKa = 10.64 GRR958 pKa = 11.84 ASSGGIDD965 pKa = 2.66 AWQFLDD971 pKa = 3.51 ILRR974 pKa = 11.84 GDD976 pKa = 3.78 RR977 pKa = 11.84 DD978 pKa = 3.95 LGSGQYY984 pKa = 11.27 GNDD987 pKa = 3.26 IEE989 pKa = 4.45 KK990 pKa = 9.62 WCAFLNRR997 pKa = 11.84 NALSRR1002 pKa = 11.84 SLRR1005 pKa = 11.84 PARR1008 pKa = 11.84 RR1009 pKa = 11.84 FRR1011 pKa = 11.84 QGTAPVEE1018 pKa = 3.86 PDD1020 pKa = 2.88 AKK1022 pKa = 10.63 GRR1024 pKa = 11.84 EE1025 pKa = 4.3 SLSRR1029 pKa = 11.84 LALLYY1034 pKa = 8.45 EE1035 pKa = 4.56 VPSSDD1040 pKa = 3.59 RR1041 pKa = 11.84 KK1042 pKa = 10.46 DD1043 pKa = 3.42 SRR1045 pKa = 11.84 RR1046 pKa = 11.84 LNFISRR1052 pKa = 11.84 ISVMLKK1058 pKa = 9.95 QFVSS1062 pKa = 3.52
Molecular weight: 121.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.492
IPC2_protein 8.375
IPC_protein 8.214
Toseland 8.726
ProMoST 8.814
Dawson 9.18
Bjellqvist 9.18
Wikipedia 9.297
Rodwell 9.355
Grimsley 9.063
Solomon 9.224
Lehninger 9.194
Nozaki 9.355
DTASelect 9.019
Thurlkill 9.136
EMBOSS 9.341
Sillero 9.341
Patrickios 4.813
IPC_peptide 9.209
IPC2_peptide 7.936
IPC2.peptide.svr19 7.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1457
395
1062
728.5
83.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.55 ± 0.772
2.265 ± 0.132
6.314 ± 0.757
7.001 ± 0.543
3.706 ± 0.579
5.353 ± 0.518
1.853 ± 0.165
5.148 ± 0.291
7.824 ± 1.635
10.364 ± 0.991
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.99 ± 0.482
3.912 ± 0.556
4.53 ± 0.361
3.02 ± 0.259
8.305 ± 0.1
7.344 ± 0.499
2.951 ± 0.168
6.108 ± 0.391
1.51 ± 0.37
2.951 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here