Pacific flying fox faeces associated circular DNA virus-9

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140CTJ0|A0A140CTJ0_9VIRU Uncharacterized protein OS=Pacific flying fox faeces associated circular DNA virus-9 OX=1796018 PE=4 SV=1
MM1 pKa = 7.27FAKK4 pKa = 10.43FWLITAHH11 pKa = 6.69FDD13 pKa = 3.57EE14 pKa = 5.83EE15 pKa = 4.47EE16 pKa = 3.98FLDD19 pKa = 5.23LVTTEE24 pKa = 4.71LGEE27 pKa = 4.06LSPVIRR33 pKa = 11.84FVWWQLEE40 pKa = 3.96AGEE43 pKa = 4.39QRR45 pKa = 11.84GSLHH49 pKa = 4.86VQAYY53 pKa = 9.93LGLHH57 pKa = 6.1RR58 pKa = 11.84SQRR61 pKa = 11.84LSYY64 pKa = 10.95VVGLFDD70 pKa = 3.72GASRR74 pKa = 11.84PVHH77 pKa = 6.0AEE79 pKa = 3.52PRR81 pKa = 11.84RR82 pKa = 11.84GTHH85 pKa = 5.13EE86 pKa = 3.94QAIQYY91 pKa = 7.4CTKK94 pKa = 10.24EE95 pKa = 4.0DD96 pKa = 3.38TRR98 pKa = 11.84RR99 pKa = 11.84AGPWFLGQPEE109 pKa = 4.35EE110 pKa = 4.29TGPGQSNALLDD121 pKa = 3.67IRR123 pKa = 11.84DD124 pKa = 3.84GLDD127 pKa = 2.96RR128 pKa = 11.84GVTLVEE134 pKa = 5.17LARR137 pKa = 11.84QDD139 pKa = 3.93NLFGALIRR147 pKa = 11.84NIRR150 pKa = 11.84GLQWYY155 pKa = 8.07AAQTGVDD162 pKa = 4.16RR163 pKa = 11.84NWEE166 pKa = 4.06TTVIILSGAPGVGKK180 pKa = 10.47SRR182 pKa = 11.84AAEE185 pKa = 3.66AAARR189 pKa = 11.84IVGVPYY195 pKa = 10.87YY196 pKa = 9.45LTRR199 pKa = 11.84PQAANSLPWWDD210 pKa = 4.48GYY212 pKa = 9.63TPGAPVVIDD221 pKa = 5.74DD222 pKa = 4.36FYY224 pKa = 11.95GWIKK228 pKa = 10.6LDD230 pKa = 3.33TMLRR234 pKa = 11.84LMDD237 pKa = 5.03RR238 pKa = 11.84YY239 pKa = 10.14PMQVEE244 pKa = 4.68TKK246 pKa = 10.63GGMLKK251 pKa = 9.29FTSRR255 pKa = 11.84LMIVTSNYY263 pKa = 7.27EE264 pKa = 3.27WRR266 pKa = 11.84NWYY269 pKa = 8.02RR270 pKa = 11.84TAGNAHH276 pKa = 6.76FPRR279 pKa = 11.84NFEE282 pKa = 3.74RR283 pKa = 11.84RR284 pKa = 11.84INRR287 pKa = 11.84DD288 pKa = 2.29IYY290 pKa = 8.79MTAEE294 pKa = 4.48DD295 pKa = 5.3DD296 pKa = 3.73YY297 pKa = 11.68LDD299 pKa = 3.36IVRR302 pKa = 11.84SILQVYY308 pKa = 10.62NDD310 pKa = 3.64MHH312 pKa = 6.23NTFHH316 pKa = 7.34WICDD320 pKa = 3.82YY321 pKa = 11.4VV322 pKa = 3.32

Molecular weight: 37.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140CTJ1|A0A140CTJ1_9VIRU Putative capsid protein OS=Pacific flying fox faeces associated circular DNA virus-9 OX=1796018 PE=4 SV=1
MM1 pKa = 6.67TAYY4 pKa = 10.18RR5 pKa = 11.84QQRR8 pKa = 11.84QQQYY12 pKa = 9.45RR13 pKa = 11.84QQQYY17 pKa = 8.85LQYY20 pKa = 8.45RR21 pKa = 11.84QFWRR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 7.36SRR29 pKa = 11.84NMPAKK34 pKa = 9.75RR35 pKa = 11.84VRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84KK41 pKa = 7.69STYY44 pKa = 6.87TRR46 pKa = 11.84RR47 pKa = 11.84ARR49 pKa = 11.84ARR51 pKa = 11.84RR52 pKa = 11.84TDD54 pKa = 2.83MRR56 pKa = 11.84TQRR59 pKa = 11.84MIVNSFYY66 pKa = 11.11GRR68 pKa = 11.84SEE70 pKa = 4.08TKK72 pKa = 10.4LLQVRR77 pKa = 11.84SGAYY81 pKa = 9.39LVNGAVGDD89 pKa = 3.89GLRR92 pKa = 11.84VDD94 pKa = 3.86SSLVQPVYY102 pKa = 11.15GKK104 pKa = 8.42ITQGSAGDD112 pKa = 3.61QRR114 pKa = 11.84IGNQIFVKK122 pKa = 9.94TISICFTIANNSFLTVDD139 pKa = 3.22QPMINGQTNVGYY151 pKa = 8.9PIPVKK156 pKa = 10.91NNIAGRR162 pKa = 11.84PDD164 pKa = 3.31RR165 pKa = 11.84GMVLQPKK172 pKa = 9.62DD173 pKa = 3.33SKK175 pKa = 11.45YY176 pKa = 10.6DD177 pKa = 3.49QEE179 pKa = 4.5GSKK182 pKa = 10.31FLPYY186 pKa = 9.68LTGFLAGTAMNAQHH200 pKa = 7.33DD201 pKa = 4.25LRR203 pKa = 11.84YY204 pKa = 10.81VNVLYY209 pKa = 10.9DD210 pKa = 3.75RR211 pKa = 11.84MEE213 pKa = 4.21VMPSTWSRR221 pKa = 11.84NNSIVGSRR229 pKa = 11.84STKK232 pKa = 9.64IVRR235 pKa = 11.84KK236 pKa = 9.41INKK239 pKa = 8.78KK240 pKa = 7.91VQYY243 pKa = 8.85EE244 pKa = 4.06QGAGTFVKK252 pKa = 10.42GGEE255 pKa = 4.23VFLCIMPGFSVADD268 pKa = 3.82PSTAVTGADD277 pKa = 3.28NKK279 pKa = 10.77PSFFSQMYY287 pKa = 10.45VSFTDD292 pKa = 3.3SS293 pKa = 2.84

Molecular weight: 33.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

876

261

322

292.0

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.708 ± 1.147

2.169 ± 1.508

3.881 ± 1.577

2.968 ± 1.423

4.452 ± 0.251

6.279 ± 1.682

2.283 ± 0.897

6.621 ± 1.557

3.881 ± 0.932

7.306 ± 1.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.511 ± 0.61

4.224 ± 0.484

4.566 ± 0.178

5.594 ± 0.998

8.447 ± 0.826

6.735 ± 1.493

7.42 ± 1.079

6.621 ± 0.747

1.941 ± 0.816

6.393 ± 1.376
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski