Amino acid dipepetide frequency for Gordonia phage SteamedHams

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.626AlaAla: 10.626 ± 1.324
0.567AlaCys: 0.567 ± 0.197
5.809AlaAsp: 5.809 ± 0.839
5.809AlaGlu: 5.809 ± 0.726
3.613AlaPhe: 3.613 ± 0.857
8.501AlaGly: 8.501 ± 0.809
1.559AlaHis: 1.559 ± 0.283
5.951AlaIle: 5.951 ± 0.623
3.967AlaLys: 3.967 ± 0.613
8.43AlaLeu: 8.43 ± 0.872
2.763AlaMet: 2.763 ± 0.523
2.409AlaAsn: 2.409 ± 0.482
5.101AlaPro: 5.101 ± 0.604
2.692AlaGln: 2.692 ± 0.481
5.03AlaArg: 5.03 ± 0.636
6.022AlaSer: 6.022 ± 0.874
6.234AlaThr: 6.234 ± 0.532
6.022AlaVal: 6.022 ± 1.099
2.267AlaTrp: 2.267 ± 0.315
2.975AlaTyr: 2.975 ± 0.324
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.286
0.071CysCys: 0.071 ± 0.077
0.638CysAsp: 0.638 ± 0.201
0.283CysGlu: 0.283 ± 0.155
0.567CysPhe: 0.567 ± 0.202
0.85CysGly: 0.85 ± 0.282
0.213CysHis: 0.213 ± 0.114
0.142CysIle: 0.142 ± 0.108
0.213CysLys: 0.213 ± 0.147
0.567CysLeu: 0.567 ± 0.192
0.142CysMet: 0.142 ± 0.113
0.142CysAsn: 0.142 ± 0.098
0.638CysPro: 0.638 ± 0.206
0.213CysGln: 0.213 ± 0.121
0.425CysArg: 0.425 ± 0.164
0.779CysSer: 0.779 ± 0.231
0.496CysThr: 0.496 ± 0.194
0.283CysVal: 0.283 ± 0.178
0.213CysTrp: 0.213 ± 0.119
0.425CysTyr: 0.425 ± 0.187
0.0CysXaa: 0.0 ± 0.0
Asp
5.88AspAla: 5.88 ± 0.651
0.779AspCys: 0.779 ± 0.26
3.896AspAsp: 3.896 ± 0.707
5.596AspGlu: 5.596 ± 0.766
1.984AspPhe: 1.984 ± 0.417
4.888AspGly: 4.888 ± 0.652
1.204AspHis: 1.204 ± 0.267
3.259AspIle: 3.259 ± 0.403
2.621AspLys: 2.621 ± 0.421
6.801AspLeu: 6.801 ± 0.861
1.771AspMet: 1.771 ± 0.443
1.629AspAsn: 1.629 ± 0.285
4.109AspPro: 4.109 ± 0.528
1.629AspGln: 1.629 ± 0.261
3.825AspArg: 3.825 ± 0.467
3.825AspSer: 3.825 ± 0.638
2.621AspThr: 2.621 ± 0.454
3.117AspVal: 3.117 ± 0.631
1.133AspTrp: 1.133 ± 0.242
1.984AspTyr: 1.984 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
6.588GluAla: 6.588 ± 0.611
0.567GluCys: 0.567 ± 0.202
5.455GluAsp: 5.455 ± 0.76
5.455GluGlu: 5.455 ± 0.861
1.488GluPhe: 1.488 ± 0.238
3.967GluGly: 3.967 ± 0.426
1.488GluHis: 1.488 ± 0.323
2.267GluIle: 2.267 ± 0.473
2.409GluLys: 2.409 ± 0.574
5.101GluLeu: 5.101 ± 0.696
1.7GluMet: 1.7 ± 0.391
1.842GluAsn: 1.842 ± 0.392
2.55GluPro: 2.55 ± 0.487
2.905GluGln: 2.905 ± 0.405
4.959GluArg: 4.959 ± 0.717
2.621GluSer: 2.621 ± 0.43
2.479GluThr: 2.479 ± 0.322
4.534GluVal: 4.534 ± 0.669
1.204GluTrp: 1.204 ± 0.298
2.621GluTyr: 2.621 ± 0.559
0.0GluXaa: 0.0 ± 0.0
Phe
2.55PheAla: 2.55 ± 0.395
0.567PheCys: 0.567 ± 0.253
2.692PheAsp: 2.692 ± 0.469
1.559PheGlu: 1.559 ± 0.312
0.85PhePhe: 0.85 ± 0.235
1.771PheGly: 1.771 ± 0.343
0.567PheHis: 0.567 ± 0.175
2.196PheIle: 2.196 ± 0.4
1.913PheLys: 1.913 ± 0.384
1.842PheLeu: 1.842 ± 0.344
0.779PheMet: 0.779 ± 0.223
0.921PheAsn: 0.921 ± 0.226
1.417PhePro: 1.417 ± 0.33
0.779PheGln: 0.779 ± 0.256
2.409PheArg: 2.409 ± 0.427
2.125PheSer: 2.125 ± 0.732
2.692PheThr: 2.692 ± 0.472
1.771PheVal: 1.771 ± 0.311
0.638PheTrp: 0.638 ± 0.196
0.567PheTyr: 0.567 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
6.942GlyAla: 6.942 ± 0.905
0.779GlyCys: 0.779 ± 0.273
6.942GlyAsp: 6.942 ± 1.01
4.25GlyGlu: 4.25 ± 0.519
2.905GlyPhe: 2.905 ± 0.365
8.572GlyGly: 8.572 ± 1.731
1.629GlyHis: 1.629 ± 0.415
3.825GlyIle: 3.825 ± 0.556
4.888GlyLys: 4.888 ± 0.656
4.746GlyLeu: 4.746 ± 0.736
2.125GlyMet: 2.125 ± 0.379
2.125GlyAsn: 2.125 ± 0.305
3.4GlyPro: 3.4 ± 0.732
3.825GlyGln: 3.825 ± 0.62
5.313GlyArg: 5.313 ± 0.758
6.234GlySer: 6.234 ± 1.065
5.455GlyThr: 5.455 ± 0.606
6.305GlyVal: 6.305 ± 0.702
2.196GlyTrp: 2.196 ± 0.471
3.188GlyTyr: 3.188 ± 0.49
0.0GlyXaa: 0.0 ± 0.0
His
1.913HisAla: 1.913 ± 0.315
0.283HisCys: 0.283 ± 0.161
1.204HisAsp: 1.204 ± 0.225
1.204HisGlu: 1.204 ± 0.341
0.638HisPhe: 0.638 ± 0.243
1.204HisGly: 1.204 ± 0.349
0.496HisHis: 0.496 ± 0.177
0.638HisIle: 0.638 ± 0.212
0.85HisLys: 0.85 ± 0.213
1.629HisLeu: 1.629 ± 0.325
0.921HisMet: 0.921 ± 0.278
0.708HisAsn: 0.708 ± 0.188
1.346HisPro: 1.346 ± 0.317
0.708HisGln: 0.708 ± 0.197
1.417HisArg: 1.417 ± 0.266
1.275HisSer: 1.275 ± 0.328
1.7HisThr: 1.7 ± 0.303
0.779HisVal: 0.779 ± 0.25
0.283HisTrp: 0.283 ± 0.134
0.283HisTyr: 0.283 ± 0.133
0.0HisXaa: 0.0 ± 0.0
Ile
4.605IleAla: 4.605 ± 0.669
0.071IleCys: 0.071 ± 0.076
3.33IleAsp: 3.33 ± 0.388
3.259IleGlu: 3.259 ± 0.494
0.779IlePhe: 0.779 ± 0.247
4.321IleGly: 4.321 ± 0.773
1.133IleHis: 1.133 ± 0.285
2.621IleIle: 2.621 ± 0.45
2.409IleLys: 2.409 ± 0.62
2.621IleLeu: 2.621 ± 0.487
0.708IleMet: 0.708 ± 0.219
1.346IleAsn: 1.346 ± 0.289
3.33IlePro: 3.33 ± 0.517
1.629IleGln: 1.629 ± 0.346
3.967IleArg: 3.967 ± 0.455
3.046IleSer: 3.046 ± 0.521
3.117IleThr: 3.117 ± 0.445
3.755IleVal: 3.755 ± 0.408
0.85IleTrp: 0.85 ± 0.269
1.346IleTyr: 1.346 ± 0.365
0.0IleXaa: 0.0 ± 0.0
Lys
5.384LysAla: 5.384 ± 0.594
0.354LysCys: 0.354 ± 0.191
3.046LysAsp: 3.046 ± 0.414
3.117LysGlu: 3.117 ± 0.39
2.125LysPhe: 2.125 ± 0.418
4.463LysGly: 4.463 ± 0.557
0.85LysHis: 0.85 ± 0.224
1.629LysIle: 1.629 ± 0.368
3.471LysLys: 3.471 ± 0.571
4.817LysLeu: 4.817 ± 0.53
1.204LysMet: 1.204 ± 0.285
2.196LysAsn: 2.196 ± 0.329
2.692LysPro: 2.692 ± 0.445
2.267LysGln: 2.267 ± 0.548
3.896LysArg: 3.896 ± 0.628
3.259LysSer: 3.259 ± 0.466
2.905LysThr: 2.905 ± 0.496
3.117LysVal: 3.117 ± 0.513
0.779LysTrp: 0.779 ± 0.254
1.204LysTyr: 1.204 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
8.643LeuAla: 8.643 ± 1.025
0.638LeuCys: 0.638 ± 0.221
4.321LeuAsp: 4.321 ± 0.574
5.596LeuGlu: 5.596 ± 0.766
1.488LeuPhe: 1.488 ± 0.354
6.305LeuGly: 6.305 ± 0.911
1.488LeuHis: 1.488 ± 0.337
3.825LeuIle: 3.825 ± 0.488
4.888LeuLys: 4.888 ± 0.513
7.722LeuLeu: 7.722 ± 0.706
2.196LeuMet: 2.196 ± 0.277
1.913LeuAsn: 1.913 ± 0.359
4.888LeuPro: 4.888 ± 0.543
2.55LeuGln: 2.55 ± 0.42
6.376LeuArg: 6.376 ± 0.607
4.392LeuSer: 4.392 ± 0.661
4.392LeuThr: 4.392 ± 0.525
5.738LeuVal: 5.738 ± 0.502
1.417LeuTrp: 1.417 ± 0.375
1.984LeuTyr: 1.984 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.834MetAla: 2.834 ± 0.377
0.0MetCys: 0.0 ± 0.0
1.275MetAsp: 1.275 ± 0.294
1.346MetGlu: 1.346 ± 0.301
1.275MetPhe: 1.275 ± 0.288
1.771MetGly: 1.771 ± 0.396
0.496MetHis: 0.496 ± 0.21
2.267MetIle: 2.267 ± 0.343
1.275MetLys: 1.275 ± 0.356
1.771MetLeu: 1.771 ± 0.341
0.85MetMet: 0.85 ± 0.205
0.921MetAsn: 0.921 ± 0.281
1.488MetPro: 1.488 ± 0.302
1.133MetGln: 1.133 ± 0.247
1.204MetArg: 1.204 ± 0.296
2.267MetSer: 2.267 ± 0.419
2.054MetThr: 2.054 ± 0.383
1.842MetVal: 1.842 ± 0.44
0.708MetTrp: 0.708 ± 0.216
0.283MetTyr: 0.283 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
2.905AsnAla: 2.905 ± 0.46
0.283AsnCys: 0.283 ± 0.159
1.488AsnAsp: 1.488 ± 0.352
1.417AsnGlu: 1.417 ± 0.303
0.992AsnPhe: 0.992 ± 0.338
3.613AsnGly: 3.613 ± 0.589
0.425AsnHis: 0.425 ± 0.141
0.992AsnIle: 0.992 ± 0.384
1.559AsnLys: 1.559 ± 0.376
1.7AsnLeu: 1.7 ± 0.299
1.133AsnMet: 1.133 ± 0.266
0.708AsnAsn: 0.708 ± 0.286
3.33AsnPro: 3.33 ± 0.564
1.063AsnGln: 1.063 ± 0.229
2.338AsnArg: 2.338 ± 0.439
1.488AsnSer: 1.488 ± 0.313
1.629AsnThr: 1.629 ± 0.289
2.479AsnVal: 2.479 ± 0.393
0.567AsnTrp: 0.567 ± 0.22
0.85AsnTyr: 0.85 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
4.888ProAla: 4.888 ± 0.67
0.567ProCys: 0.567 ± 0.227
3.613ProAsp: 3.613 ± 0.583
3.4ProGlu: 3.4 ± 0.554
1.488ProPhe: 1.488 ± 0.353
5.738ProGly: 5.738 ± 0.588
1.204ProHis: 1.204 ± 0.246
2.054ProIle: 2.054 ± 0.408
3.259ProLys: 3.259 ± 0.565
4.605ProLeu: 4.605 ± 0.595
1.346ProMet: 1.346 ± 0.346
1.984ProAsn: 1.984 ± 0.334
2.479ProPro: 2.479 ± 0.458
2.479ProGln: 2.479 ± 0.462
2.338ProArg: 2.338 ± 0.408
2.834ProSer: 2.834 ± 0.411
3.259ProThr: 3.259 ± 0.391
3.613ProVal: 3.613 ± 0.423
1.346ProTrp: 1.346 ± 0.304
1.275ProTyr: 1.275 ± 0.289
0.0ProXaa: 0.0 ± 0.0
Gln
3.471GlnAla: 3.471 ± 0.654
0.283GlnCys: 0.283 ± 0.194
1.417GlnAsp: 1.417 ± 0.305
1.771GlnGlu: 1.771 ± 0.293
1.7GlnPhe: 1.7 ± 0.366
3.046GlnGly: 3.046 ± 0.524
0.779GlnHis: 0.779 ± 0.277
2.125GlnIle: 2.125 ± 0.428
1.984GlnLys: 1.984 ± 0.343
3.046GlnLeu: 3.046 ± 0.409
0.638GlnMet: 0.638 ± 0.203
1.204GlnAsn: 1.204 ± 0.38
1.7GlnPro: 1.7 ± 0.368
1.559GlnGln: 1.559 ± 0.443
2.692GlnArg: 2.692 ± 0.346
2.338GlnSer: 2.338 ± 0.382
1.771GlnThr: 1.771 ± 0.371
2.409GlnVal: 2.409 ± 0.34
1.204GlnTrp: 1.204 ± 0.261
1.275GlnTyr: 1.275 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
5.809ArgAla: 5.809 ± 0.652
0.425ArgCys: 0.425 ± 0.219
3.755ArgAsp: 3.755 ± 0.606
4.676ArgGlu: 4.676 ± 0.443
2.409ArgPhe: 2.409 ± 0.393
5.596ArgGly: 5.596 ± 0.749
0.708ArgHis: 0.708 ± 0.22
2.338ArgIle: 2.338 ± 0.4
4.888ArgLys: 4.888 ± 0.672
5.171ArgLeu: 5.171 ± 0.685
2.054ArgMet: 2.054 ± 0.464
1.771ArgAsn: 1.771 ± 0.393
2.55ArgPro: 2.55 ± 0.458
2.479ArgGln: 2.479 ± 0.434
4.534ArgArg: 4.534 ± 0.708
3.33ArgSer: 3.33 ± 0.461
3.33ArgThr: 3.33 ± 0.519
5.455ArgVal: 5.455 ± 0.817
2.054ArgTrp: 2.054 ± 0.445
1.7ArgTyr: 1.7 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
5.667SerAla: 5.667 ± 0.895
0.354SerCys: 0.354 ± 0.163
3.117SerAsp: 3.117 ± 0.492
3.613SerGlu: 3.613 ± 0.661
1.275SerPhe: 1.275 ± 0.26
6.801SerGly: 6.801 ± 0.625
1.488SerHis: 1.488 ± 0.35
3.613SerIle: 3.613 ± 0.525
2.479SerLys: 2.479 ± 0.451
4.888SerLeu: 4.888 ± 0.62
1.7SerMet: 1.7 ± 0.31
2.196SerAsn: 2.196 ± 0.364
3.4SerPro: 3.4 ± 0.617
2.692SerGln: 2.692 ± 0.514
3.4SerArg: 3.4 ± 0.387
3.684SerSer: 3.684 ± 0.508
3.542SerThr: 3.542 ± 0.658
3.33SerVal: 3.33 ± 0.501
1.559SerTrp: 1.559 ± 0.349
1.346SerTyr: 1.346 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
6.234ThrAla: 6.234 ± 0.809
0.213ThrCys: 0.213 ± 0.144
3.188ThrAsp: 3.188 ± 0.723
2.196ThrGlu: 2.196 ± 0.389
1.488ThrPhe: 1.488 ± 0.293
5.384ThrGly: 5.384 ± 0.654
0.708ThrHis: 0.708 ± 0.204
2.975ThrIle: 2.975 ± 0.453
3.825ThrLys: 3.825 ± 0.522
4.959ThrLeu: 4.959 ± 0.554
1.629ThrMet: 1.629 ± 0.351
2.409ThrAsn: 2.409 ± 0.304
4.109ThrPro: 4.109 ± 0.485
1.275ThrGln: 1.275 ± 0.289
4.18ThrArg: 4.18 ± 0.523
2.409ThrSer: 2.409 ± 0.44
4.109ThrThr: 4.109 ± 0.906
5.738ThrVal: 5.738 ± 0.547
0.992ThrTrp: 0.992 ± 0.247
1.346ThrTyr: 1.346 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
6.447ValAla: 6.447 ± 0.649
0.567ValCys: 0.567 ± 0.207
3.755ValAsp: 3.755 ± 0.542
4.25ValGlu: 4.25 ± 0.519
1.913ValPhe: 1.913 ± 0.318
5.101ValGly: 5.101 ± 0.754
1.488ValHis: 1.488 ± 0.319
3.684ValIle: 3.684 ± 0.49
3.259ValLys: 3.259 ± 0.444
5.384ValLeu: 5.384 ± 0.531
1.771ValMet: 1.771 ± 0.35
2.834ValAsn: 2.834 ± 0.42
3.542ValPro: 3.542 ± 0.596
2.338ValGln: 2.338 ± 0.322
3.33ValArg: 3.33 ± 0.438
5.03ValSer: 5.03 ± 0.57
5.455ValThr: 5.455 ± 0.583
4.605ValVal: 4.605 ± 0.631
2.125ValTrp: 2.125 ± 0.339
2.479ValTyr: 2.479 ± 0.539
0.0ValXaa: 0.0 ± 0.0
Trp
2.479TrpAla: 2.479 ± 0.409
0.496TrpCys: 0.496 ± 0.16
1.346TrpAsp: 1.346 ± 0.276
1.417TrpGlu: 1.417 ± 0.267
0.638TrpPhe: 0.638 ± 0.21
1.346TrpGly: 1.346 ± 0.446
0.638TrpHis: 0.638 ± 0.223
0.354TrpIle: 0.354 ± 0.126
0.992TrpLys: 0.992 ± 0.258
2.479TrpLeu: 2.479 ± 0.417
0.496TrpMet: 0.496 ± 0.18
1.063TrpAsn: 1.063 ± 0.342
0.708TrpPro: 0.708 ± 0.243
1.346TrpGln: 1.346 ± 0.337
1.133TrpArg: 1.133 ± 0.226
1.204TrpSer: 1.204 ± 0.269
0.85TrpThr: 0.85 ± 0.204
1.842TrpVal: 1.842 ± 0.332
0.425TrpTrp: 0.425 ± 0.19
0.992TrpTyr: 0.992 ± 0.265
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.7TyrAla: 1.7 ± 0.329
0.213TyrCys: 0.213 ± 0.099
2.267TyrAsp: 2.267 ± 0.383
1.984TyrGlu: 1.984 ± 0.351
0.992TyrPhe: 0.992 ± 0.236
2.409TyrGly: 2.409 ± 0.381
0.921TyrHis: 0.921 ± 0.302
1.488TyrIle: 1.488 ± 0.284
1.629TyrLys: 1.629 ± 0.332
2.479TyrLeu: 2.479 ± 0.485
1.133TyrMet: 1.133 ± 0.296
0.638TyrAsn: 0.638 ± 0.204
0.992TyrPro: 0.992 ± 0.285
0.85TyrGln: 0.85 ± 0.198
2.267TyrArg: 2.267 ± 0.396
1.984TyrSer: 1.984 ± 0.412
1.275TyrThr: 1.275 ± 0.294
2.621TyrVal: 2.621 ± 0.389
0.283TyrTrp: 0.283 ± 0.16
0.85TyrTyr: 0.85 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (14117 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski