Phaeobacter phage MD18

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H1Z652|A0A6H1Z652_9CAUD Uncharacterized protein OS=Phaeobacter phage MD18 OX=2723761 PE=4 SV=1
MM1 pKa = 7.72LYY3 pKa = 10.74VFLLTIYY10 pKa = 9.69TEE12 pKa = 4.2TSIDD16 pKa = 3.78VYY18 pKa = 11.61VEE20 pKa = 4.07DD21 pKa = 4.91SGLTGTDD28 pKa = 3.56CIEE31 pKa = 3.86AMEE34 pKa = 5.18NYY36 pKa = 10.26SQTNPSWTDD45 pKa = 3.38GNPSCEE51 pKa = 4.15PDD53 pKa = 3.76HH54 pKa = 6.55ATMPAEE60 pKa = 4.35IKK62 pKa = 10.23HH63 pKa = 5.47QGEE66 pKa = 4.6IYY68 pKa = 10.64VLPACDD74 pKa = 4.22TEE76 pKa = 5.42DD77 pKa = 4.58SSNCYY82 pKa = 8.75WDD84 pKa = 3.42AGTRR88 pKa = 11.84GNGEE92 pKa = 4.24GISFYY97 pKa = 10.79DD98 pKa = 2.95IGGNAFLLYY107 pKa = 10.06PP108 pKa = 4.62

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H1Z6B8|A0A6H1Z6B8_9CAUD Uncharacterized protein OS=Phaeobacter phage MD18 OX=2723761 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84KK3 pKa = 8.51PAPIRR8 pKa = 11.84IWRR11 pKa = 11.84DD12 pKa = 2.89PTRR15 pKa = 11.84RR16 pKa = 11.84DD17 pKa = 3.25VEE19 pKa = 4.25EE20 pKa = 4.14LCRR23 pKa = 11.84EE24 pKa = 3.97LGGEE28 pKa = 4.13LFVRR32 pKa = 11.84PAYY35 pKa = 10.07DD36 pKa = 3.28GHH38 pKa = 7.18RR39 pKa = 11.84LGAGARR45 pKa = 11.84SFYY48 pKa = 10.17KK49 pKa = 10.23RR50 pKa = 11.84SVVDD54 pKa = 4.02GVTVNMWIGFARR66 pKa = 11.84KK67 pKa = 8.34GQRR70 pKa = 11.84PDD72 pKa = 2.68WRR74 pKa = 11.84DD75 pKa = 3.08MLKK78 pKa = 10.58RR79 pKa = 11.84LGSDD83 pKa = 2.98WAA85 pKa = 4.46

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

42325

29

1815

184.8

20.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.97 ± 0.388

1.032 ± 0.082

6.781 ± 0.208

7.506 ± 0.184

3.868 ± 0.104

7.844 ± 0.201

2.259 ± 0.117

4.891 ± 0.111

4.702 ± 0.158

7.286 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.968 ± 0.103

3.438 ± 0.101

4.473 ± 0.153

2.949 ± 0.164

6.573 ± 0.164

5.81 ± 0.21

5.864 ± 0.164

7.204 ± 0.157

1.67 ± 0.077

2.913 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski