Leishmania major

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania; Leishmania; Leishmania major species complex

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O97204|O97204_LEIMA Uncharacterized protein OS=Leishmania major OX=5664 GN=LMJF_04_0510 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.49YY4 pKa = 11.4SSDD7 pKa = 3.96DD8 pKa = 3.33EE9 pKa = 4.82DD10 pKa = 4.37VVVLQVCANRR20 pKa = 11.84HH21 pKa = 4.79CQGIDD26 pKa = 3.15DD27 pKa = 5.04LEE29 pKa = 5.2FDD31 pKa = 3.95EE32 pKa = 5.12EE33 pKa = 4.87TNQSYY38 pKa = 10.79CGRR41 pKa = 11.84CRR43 pKa = 11.84ALYY46 pKa = 10.61ARR48 pKa = 11.84TEE50 pKa = 4.3TEE52 pKa = 3.84GFRR55 pKa = 11.84VLLSNDD61 pKa = 3.21DD62 pKa = 3.86LGLVKK67 pKa = 10.81LIFDD71 pKa = 3.67QFDD74 pKa = 3.55EE75 pKa = 4.26NSRR78 pKa = 11.84GFWTYY83 pKa = 11.29KK84 pKa = 8.83EE85 pKa = 3.87WNAFQEE91 pKa = 4.43VTEE94 pKa = 4.97RR95 pKa = 11.84GSDD98 pKa = 3.68DD99 pKa = 4.62PIEE102 pKa = 4.43SSAALKK108 pKa = 10.68EE109 pKa = 4.33FFKK112 pKa = 11.07EE113 pKa = 4.32EE114 pKa = 3.83YY115 pKa = 10.49DD116 pKa = 3.47IDD118 pKa = 3.71ISAHH122 pKa = 5.56GKK124 pKa = 10.13DD125 pKa = 3.6GAVVLLVDD133 pKa = 5.01LEE135 pKa = 4.13NMYY138 pKa = 10.99GGYY141 pKa = 10.52LYY143 pKa = 11.21NNVDD147 pKa = 3.65ALVEE151 pKa = 4.18DD152 pKa = 4.41SEE154 pKa = 4.4AMEE157 pKa = 4.28YY158 pKa = 10.27QGLLHH163 pKa = 6.41TGVLEE168 pKa = 4.11

Molecular weight: 19.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q4Q1G9|Q4Q1G9_LEIMA Uncharacterized protein OS=Leishmania major OX=5664 GN=LMJF_36_2520 PE=4 SV=1
MM1 pKa = 7.63AKK3 pKa = 10.06SKK5 pKa = 10.65NHH7 pKa = 5.76TNHH10 pKa = 5.7NQSSKK15 pKa = 7.51NHH17 pKa = 6.0RR18 pKa = 11.84NGIKK22 pKa = 10.6GPVPLHH28 pKa = 5.48LHH30 pKa = 4.88NSKK33 pKa = 10.54RR34 pKa = 11.84GSWLPALVNARR45 pKa = 11.84RR46 pKa = 11.84VRR48 pKa = 11.84KK49 pKa = 9.49HH50 pKa = 4.74NQKK53 pKa = 10.61AALKK57 pKa = 9.79KK58 pKa = 9.32RR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 4.05RR62 pKa = 11.84IAAFAAKK69 pKa = 9.96NN70 pKa = 3.41

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8038

0

8038

5143074

31

17392

639.8

69.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.066 ± 0.051

1.892 ± 0.013

4.858 ± 0.023

5.974 ± 0.041

2.946 ± 0.018

6.457 ± 0.036

2.713 ± 0.015

2.96 ± 0.019

3.288 ± 0.023

9.195 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.242 ± 0.013

2.608 ± 0.016

5.817 ± 0.034

4.104 ± 0.025

7.185 ± 0.037

9.046 ± 0.21

6.019 ± 0.027

7.152 ± 0.032

1.08 ± 0.009

2.4 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski