Leishmania major
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8038 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O97204|O97204_LEIMA Uncharacterized protein OS=Leishmania major OX=5664 GN=LMJF_04_0510 PE=4 SV=1
MM1 pKa = 7.78 SDD3 pKa = 3.49 YY4 pKa = 11.4 SSDD7 pKa = 3.96 DD8 pKa = 3.33 EE9 pKa = 4.82 DD10 pKa = 4.37 VVVLQVCANRR20 pKa = 11.84 HH21 pKa = 4.79 CQGIDD26 pKa = 3.15 DD27 pKa = 5.04 LEE29 pKa = 5.2 FDD31 pKa = 3.95 EE32 pKa = 5.12 EE33 pKa = 4.87 TNQSYY38 pKa = 10.79 CGRR41 pKa = 11.84 CRR43 pKa = 11.84 ALYY46 pKa = 10.61 ARR48 pKa = 11.84 TEE50 pKa = 4.3 TEE52 pKa = 3.84 GFRR55 pKa = 11.84 VLLSNDD61 pKa = 3.21 DD62 pKa = 3.86 LGLVKK67 pKa = 10.81 LIFDD71 pKa = 3.67 QFDD74 pKa = 3.55 EE75 pKa = 4.26 NSRR78 pKa = 11.84 GFWTYY83 pKa = 11.29 KK84 pKa = 8.83 EE85 pKa = 3.87 WNAFQEE91 pKa = 4.43 VTEE94 pKa = 4.97 RR95 pKa = 11.84 GSDD98 pKa = 3.68 DD99 pKa = 4.62 PIEE102 pKa = 4.43 SSAALKK108 pKa = 10.68 EE109 pKa = 4.33 FFKK112 pKa = 11.07 EE113 pKa = 4.32 EE114 pKa = 3.83 YY115 pKa = 10.49 DD116 pKa = 3.47 IDD118 pKa = 3.71 ISAHH122 pKa = 5.56 GKK124 pKa = 10.13 DD125 pKa = 3.6 GAVVLLVDD133 pKa = 5.01 LEE135 pKa = 4.13 NMYY138 pKa = 10.99 GGYY141 pKa = 10.52 LYY143 pKa = 11.21 NNVDD147 pKa = 3.65 ALVEE151 pKa = 4.18 DD152 pKa = 4.41 SEE154 pKa = 4.4 AMEE157 pKa = 4.28 YY158 pKa = 10.27 QGLLHH163 pKa = 6.41 TGVLEE168 pKa = 4.11
Molecular weight: 19.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.778
IPC2_protein 3.961
IPC_protein 3.935
Toseland 3.745
ProMoST 4.062
Dawson 3.91
Bjellqvist 4.088
Wikipedia 3.821
Rodwell 3.77
Grimsley 3.656
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.215
Thurlkill 3.77
EMBOSS 3.834
Sillero 4.05
Patrickios 1.901
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|Q4Q1G9|Q4Q1G9_LEIMA Uncharacterized protein OS=Leishmania major OX=5664 GN=LMJF_36_2520 PE=4 SV=1
MM1 pKa = 7.63 AKK3 pKa = 10.06 SKK5 pKa = 10.65 NHH7 pKa = 5.76 TNHH10 pKa = 5.7 NQSSKK15 pKa = 7.51 NHH17 pKa = 6.0 RR18 pKa = 11.84 NGIKK22 pKa = 10.6 GPVPLHH28 pKa = 5.48 LHH30 pKa = 4.88 NSKK33 pKa = 10.54 RR34 pKa = 11.84 GSWLPALVNARR45 pKa = 11.84 RR46 pKa = 11.84 VRR48 pKa = 11.84 KK49 pKa = 9.49 HH50 pKa = 4.74 NQKK53 pKa = 10.61 AALKK57 pKa = 9.79 KK58 pKa = 9.32 RR59 pKa = 11.84 RR60 pKa = 11.84 EE61 pKa = 4.05 RR62 pKa = 11.84 IAAFAAKK69 pKa = 9.96 NN70 pKa = 3.41
Molecular weight: 7.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.357
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.398
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.135
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.011
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8038
0
8038
5143074
31
17392
639.8
69.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.066 ± 0.051
1.892 ± 0.013
4.858 ± 0.023
5.974 ± 0.041
2.946 ± 0.018
6.457 ± 0.036
2.713 ± 0.015
2.96 ± 0.019
3.288 ± 0.023
9.195 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.242 ± 0.013
2.608 ± 0.016
5.817 ± 0.034
4.104 ± 0.025
7.185 ± 0.037
9.046 ± 0.21
6.019 ± 0.027
7.152 ± 0.032
1.08 ± 0.009
2.4 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here