Trichomonas vaginalis
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 50190 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A2HWU2|A2HWU2_TRIVA DNA-directed DNA polymerase (Fragment) OS=Trichomonas vaginalis OX=5722 GN=TVAG_583250 PE=4 SV=1
MM1 pKa = 6.55 YY2 pKa = 9.98 TNFLRR7 pKa = 11.84 FLSSSRR13 pKa = 11.84 CFLTPSCIHH22 pKa = 6.39 HH23 pKa = 6.66 AASSLHH29 pKa = 5.76 QGASSLDD36 pKa = 3.5 QDD38 pKa = 4.49 ASSLLQSTSSLDD50 pKa = 3.39 QDD52 pKa = 3.96 ASSLLQSTSSLHH64 pKa = 5.85 QDD66 pKa = 2.9 ASSLLQSTSSLHH78 pKa = 5.94 QDD80 pKa = 3.72 ASSLDD85 pKa = 3.54 QGASSLLQSTSSLDD99 pKa = 3.39 QDD101 pKa = 3.96 ASSLLQSTSSLQQSDD116 pKa = 3.5 SSLLQNTSSLDD127 pKa = 3.39 QGASSLLQSTSSLDD141 pKa = 3.3 QGASSLHH148 pKa = 5.76 QDD150 pKa = 3.28 ASSLQQSTSSLHH162 pKa = 5.84 QDD164 pKa = 3.65 ASSLDD169 pKa = 3.57 QDD171 pKa = 4.13 ASSLLQSTSSLDD183 pKa = 3.39 QDD185 pKa = 3.96 ASSLLQSTSSLLQSTSSLHH204 pKa = 5.94 QDD206 pKa = 3.72 ASSLDD211 pKa = 3.48 QGASSLHH218 pKa = 5.73 QDD220 pKa = 3.38 ASSLLQSTSSLHH232 pKa = 5.94 QDD234 pKa = 3.65 ASSLDD239 pKa = 3.57 QDD241 pKa = 4.13 ASSLLQSTSSLHH253 pKa = 5.94 QDD255 pKa = 3.72 ASSLDD260 pKa = 3.54 QGASSLLQSTSSLHH274 pKa = 5.85 QDD276 pKa = 2.9 ASSLLQSTSSLHH288 pKa = 5.94 QDD290 pKa = 3.65 ASSLDD295 pKa = 3.57 QDD297 pKa = 4.59 ASSLLQQ303 pKa = 3.54
Molecular weight: 31.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.808
IPC_protein 3.859
Toseland 3.617
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.05
Wikipedia 3.91
Rodwell 3.694
Grimsley 3.516
Solomon 3.897
Lehninger 3.859
Nozaki 4.024
DTASelect 4.393
Thurlkill 3.694
EMBOSS 3.923
Sillero 3.999
Patrickios 1.952
IPC_peptide 3.884
IPC2_peptide 3.973
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A2HED7|A2HED7_TRIVA Uncharacterized protein (Fragment) OS=Trichomonas vaginalis OX=5722 GN=TVAG_574800 PE=4 SV=1
TT1 pKa = 5.42 TTISSSSGLFLNRR14 pKa = 11.84 RR15 pKa = 11.84 QQQSLPAQALPQQRR29 pKa = 11.84 QQQSLPAQALPQQRR43 pKa = 11.84 QQQSLPAQALPQQRR57 pKa = 11.84 QQQSLPAQTLPQQRR71 pKa = 11.84 QQQSLPAQALPQQRR85 pKa = 11.84 QQQSLPAQTLPQQRR99 pKa = 11.84 QQQSLPAQALPQQRR113 pKa = 11.84 QQQSLPAQALPQQRR127 pKa = 11.84 QQQSLPAQALPQQRR141 pKa = 11.84 QQQSLPAQALPQQRR155 pKa = 11.84 QQQSLPAQTLPQQRR169 pKa = 11.84 QQQSLPTQALPKK181 pKa = 9.11 QQPHH185 pKa = 5.28 II186 pKa = 4.33
Molecular weight: 20.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.34
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.251
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
50190
0
50190
17055200
30
9597
339.8
38.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.145 ± 0.013
1.594 ± 0.006
5.819 ± 0.009
7.384 ± 0.021
5.149 ± 0.013
3.958 ± 0.014
1.949 ± 0.006
7.943 ± 0.014
8.296 ± 0.018
8.654 ± 0.014
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.302 ± 0.005
6.423 ± 0.011
4.18 ± 0.013
4.128 ± 0.013
3.727 ± 0.008
7.555 ± 0.016
5.566 ± 0.01
5.189 ± 0.009
0.792 ± 0.004
4.07 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here