Mycobacterium phage CASbig

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JHK0|R4JHK0_9CAUD Uncharacterized protein OS=Mycobacterium phage CASbig OX=1327035 GN=CASbig_74 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLSLEE16 pKa = 4.13ITGDD20 pKa = 3.64GSAEE24 pKa = 3.94EE25 pKa = 4.62YY26 pKa = 10.64INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JN15|R4JN15_9CAUD Uncharacterized protein OS=Mycobacterium phage CASbig OX=1327035 GN=CASbig_2 PE=4 SV=1
MM1 pKa = 7.49PVDD4 pKa = 3.93AEE6 pKa = 4.52TFLGHH11 pKa = 6.49PLSRR15 pKa = 11.84VVEE18 pKa = 4.22RR19 pKa = 11.84LALSSTGKK27 pKa = 10.42AGTAIRR33 pKa = 11.84TGVVLKK39 pKa = 10.41VASRR43 pKa = 11.84EE44 pKa = 4.09LVPALAVSQSCMEE57 pKa = 3.99VRR59 pKa = 11.84AVDD62 pKa = 4.12LGVLGVLRR70 pKa = 11.84PGSVLKK76 pKa = 10.72VLQTVVVRR84 pKa = 11.84VTVQVPDD91 pKa = 5.22LISLGPGSYY100 pKa = 10.15EE101 pKa = 3.66RR102 pKa = 11.84LHH104 pKa = 6.34DD105 pKa = 3.54QRR107 pKa = 11.84VDD109 pKa = 3.28QRR111 pKa = 11.84SPRR114 pKa = 11.84GSRR117 pKa = 11.84DD118 pKa = 2.61RR119 pKa = 11.84HH120 pKa = 5.26VPVTGYY126 pKa = 10.04HH127 pKa = 6.19RR128 pKa = 11.84PGSKK132 pKa = 10.29CLTGDD137 pKa = 3.27QRR139 pKa = 11.84QDD141 pKa = 3.05LALVANQVLTLVPDD155 pKa = 3.85YY156 pKa = 7.62WTPLHH161 pKa = 6.08QRR163 pKa = 11.84LNGLMALIICSGSRR177 pKa = 11.84DD178 pKa = 3.47KK179 pKa = 11.5SGNSPMILSSSRR191 pKa = 11.84DD192 pKa = 3.25RR193 pKa = 11.84SGIFAKK199 pKa = 10.36ILSSRR204 pKa = 11.84SARR207 pKa = 11.84SGSFSVARR215 pKa = 11.84SMTWFRR221 pKa = 11.84NSGG224 pKa = 3.2

Molecular weight: 24.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

17831

34

792

183.8

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.618 ± 0.388

0.847 ± 0.112

6.668 ± 0.231

6.091 ± 0.235

3.051 ± 0.16

8.306 ± 0.408

2.187 ± 0.191

4.543 ± 0.231

3.606 ± 0.276

8.771 ± 0.289

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.126

3.185 ± 0.193

5.877 ± 0.283

3.191 ± 0.208

7.279 ± 0.538

6.562 ± 0.448

6.124 ± 0.288

7.655 ± 0.288

1.789 ± 0.105

2.619 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski