Mycobacterium phage DrDrey

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Kostyavirus; unclassified Kostyavirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Y7A5|S5Y7A5_9CAUD DNA helicase OS=Mycobacterium phage DrDrey OX=1357669 GN=81 PE=3 SV=1
MM1 pKa = 8.2DD2 pKa = 6.72DD3 pKa = 4.63DD4 pKa = 6.48DD5 pKa = 5.44LRR7 pKa = 11.84CSCGSLYY14 pKa = 10.72DD15 pKa = 4.55DD16 pKa = 5.69CDD18 pKa = 3.69PDD20 pKa = 3.87YY21 pKa = 11.58CFDD24 pKa = 3.69NPQSYY29 pKa = 9.5FYY31 pKa = 10.1PEE33 pKa = 3.83PRR35 pKa = 11.84TWDD38 pKa = 4.41DD39 pKa = 3.5IEE41 pKa = 6.61DD42 pKa = 5.19DD43 pKa = 3.46IASAFTGQMKK53 pKa = 10.58NLDD56 pKa = 3.4AAFTRR61 pKa = 11.84EE62 pKa = 4.01APKK65 pKa = 10.47YY66 pKa = 10.37APTWWMLVTACPEE79 pKa = 4.51DD80 pKa = 3.55IRR82 pKa = 11.84IEE84 pKa = 4.03EE85 pKa = 4.3DD86 pKa = 3.44VEE88 pKa = 4.8HH89 pKa = 7.19EE90 pKa = 4.67DD91 pKa = 4.95VICGDD96 pKa = 3.16IRR98 pKa = 11.84RR99 pKa = 11.84SIITNRR105 pKa = 11.84NLYY108 pKa = 9.02LAWGEE113 pKa = 4.15DD114 pKa = 3.66SRR116 pKa = 11.84HH117 pKa = 6.22PIYY120 pKa = 10.62AYY122 pKa = 8.73FQRR125 pKa = 11.84VDD127 pKa = 3.78FDD129 pKa = 4.59KK130 pKa = 10.98QLSWISDD137 pKa = 3.6VQMVEE142 pKa = 4.13PPAWGLWMPSLIGVV156 pKa = 4.11

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5YQD3|S5YQD3_9CAUD Uncharacterized protein OS=Mycobacterium phage DrDrey OX=1357669 GN=44 PE=4 SV=1
MM1 pKa = 7.58KK2 pKa = 10.19LHH4 pKa = 6.98RR5 pKa = 11.84PEE7 pKa = 4.26TQKK10 pKa = 10.97KK11 pKa = 9.41APPSLNHH18 pKa = 6.67KK19 pKa = 9.4GNAVGVSGGAHH30 pKa = 5.69DD31 pKa = 4.47TIIGEE36 pKa = 4.44VIKK39 pKa = 9.9PYY41 pKa = 10.38PPNPKK46 pKa = 10.22YY47 pKa = 10.37KK48 pKa = 10.38VSDD51 pKa = 3.65DD52 pKa = 3.26GRR54 pKa = 11.84VFGFKK59 pKa = 10.31GQEE62 pKa = 4.37LKK64 pKa = 11.17RR65 pKa = 11.84MWTPSGYY72 pKa = 9.78RR73 pKa = 11.84RR74 pKa = 11.84VNIYY78 pKa = 10.73YY79 pKa = 9.72PDD81 pKa = 3.36GTVRR85 pKa = 11.84KK86 pKa = 9.04QRR88 pKa = 11.84VHH90 pKa = 7.84RR91 pKa = 11.84MVLEE95 pKa = 4.34TFVGPCPPGMEE106 pKa = 4.13ACHH109 pKa = 6.46NNGTPGDD116 pKa = 3.83NRR118 pKa = 11.84LSNLRR123 pKa = 11.84WDD125 pKa = 3.9TPKK128 pKa = 10.97ANQADD133 pKa = 4.32KK134 pKa = 10.79VKK136 pKa = 10.24HH137 pKa = 5.62GRR139 pKa = 11.84PTSPGAAAARR149 pKa = 11.84LRR151 pKa = 11.84EE152 pKa = 3.96RR153 pKa = 11.84DD154 pKa = 3.17EE155 pKa = 4.2CRR157 pKa = 11.84NGHH160 pKa = 7.38LYY162 pKa = 9.67TPEE165 pKa = 3.58NTYY168 pKa = 7.58THH170 pKa = 7.5PSRR173 pKa = 11.84PDD175 pKa = 2.92RR176 pKa = 11.84RR177 pKa = 11.84KK178 pKa = 10.21CRR180 pKa = 11.84QCAADD185 pKa = 3.17ARR187 pKa = 11.84VRR189 pKa = 11.84YY190 pKa = 8.87KK191 pKa = 10.9ARR193 pKa = 11.84AAA195 pKa = 3.6

Molecular weight: 21.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

144

0

144

23929

24

1578

166.2

18.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.247 ± 0.344

1.162 ± 0.139

6.44 ± 0.227

6.649 ± 0.247

2.934 ± 0.164

8.759 ± 0.493

2.466 ± 0.165

4.53 ± 0.164

4.463 ± 0.2

8.153 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.131

3.381 ± 0.142

5.324 ± 0.222

3.293 ± 0.206

5.863 ± 0.316

5.612 ± 0.192

6.298 ± 0.25

6.996 ± 0.23

2.039 ± 0.136

3.047 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski