Hyphomicrobium nitrativorans NL23

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium; Hyphomicrobium nitrativorans

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3330 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5SKD9|V5SKD9_9RHIZ Uncharacterized protein OS=Hyphomicrobium nitrativorans NL23 OX=1029756 GN=W911_16835 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 10.11KK3 pKa = 10.38LCTASIAALLIATGGTAFAQVDD25 pKa = 4.17APADD29 pKa = 3.4AEE31 pKa = 4.4MQLSQAEE38 pKa = 4.49CQTIWNRR45 pKa = 11.84ADD47 pKa = 3.29AAGSGSLSAADD58 pKa = 3.52ADD60 pKa = 4.01RR61 pKa = 11.84YY62 pKa = 10.38VSNFSAADD70 pKa = 3.55TDD72 pKa = 4.23GDD74 pKa = 4.08GSLSSTEE81 pKa = 4.5FMAACDD87 pKa = 3.12QGLIHH92 pKa = 7.5DD93 pKa = 4.92SSATGAGEE101 pKa = 4.28GVQGSDD107 pKa = 3.47TLPAPEE113 pKa = 4.96GAPSQYY119 pKa = 11.58

Molecular weight: 11.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5SA50|V5SA50_9RHIZ Glycosyl transferase OS=Hyphomicrobium nitrativorans NL23 OX=1029756 GN=W911_03050 PE=4 SV=1
MM1 pKa = 7.81AKK3 pKa = 7.62TTAKK7 pKa = 10.21KK8 pKa = 10.62APARR12 pKa = 11.84KK13 pKa = 8.96AATARR18 pKa = 11.84KK19 pKa = 7.89PAARR23 pKa = 11.84KK24 pKa = 6.73TAAARR29 pKa = 11.84KK30 pKa = 7.37PAARR34 pKa = 11.84KK35 pKa = 5.12TTAARR40 pKa = 11.84KK41 pKa = 4.93TTAARR46 pKa = 11.84KK47 pKa = 7.51PAARR51 pKa = 11.84KK52 pKa = 7.94ATAARR57 pKa = 11.84KK58 pKa = 7.92PAARR62 pKa = 11.84KK63 pKa = 8.36AAKK66 pKa = 9.23PAARR70 pKa = 11.84KK71 pKa = 8.04PAARR75 pKa = 11.84KK76 pKa = 5.12TTAARR81 pKa = 11.84KK82 pKa = 7.72PAARR86 pKa = 11.84KK87 pKa = 8.3PAAKK91 pKa = 8.69RR92 pKa = 11.84TKK94 pKa = 10.12KK95 pKa = 10.45AAA97 pKa = 3.52

Molecular weight: 10.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3330

0

3330

1004392

29

2163

301.6

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.922 ± 0.054

0.785 ± 0.013

5.725 ± 0.037

6.001 ± 0.035

3.599 ± 0.029

8.486 ± 0.038

2.073 ± 0.021

4.961 ± 0.027

3.408 ± 0.039

9.838 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.022

2.479 ± 0.027

5.254 ± 0.038

2.901 ± 0.023

7.434 ± 0.046

5.486 ± 0.032

5.445 ± 0.028

7.528 ± 0.034

1.295 ± 0.016

2.15 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski