Marmoricola sp. Leaf446

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Marmoricola; unclassified Marmoricola

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6F8A3|A0A0Q6F8A3_9ACTN HTH marR-type domain-containing protein OS=Marmoricola sp. Leaf446 OX=1736379 GN=ASG49_13705 PE=4 SV=1
MM1 pKa = 7.41SMSGQFDD8 pKa = 3.99VVTAEE13 pKa = 3.89LTAAADD19 pKa = 5.02RR20 pKa = 11.84IRR22 pKa = 11.84SAVAPVACYY31 pKa = 8.07TLPSTGASATAVGHH45 pKa = 6.07DD46 pKa = 3.45QLGEE50 pKa = 4.15VVTAFCEE57 pKa = 4.04KK58 pKa = 10.19VGQVVSATALGDD70 pKa = 3.32EE71 pKa = 4.46AAAGEE76 pKa = 4.43LDD78 pKa = 3.84ASAAAYY84 pKa = 8.25DD85 pKa = 3.8ATDD88 pKa = 3.48AANQAVYY95 pKa = 8.94TAMNPFGLPVASPRR109 pKa = 3.63

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6FA81|A0A0Q6FA81_9ACTN Polyisoprenoid-binding protein OS=Marmoricola sp. Leaf446 OX=1736379 GN=ASG49_11045 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 4.64KK15 pKa = 8.79THH17 pKa = 6.48GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 10.13GRR40 pKa = 11.84GRR42 pKa = 11.84LAAA45 pKa = 4.78

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3454

0

3454

1121115

37

6029

324.6

34.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.083 ± 0.063

0.689 ± 0.011

6.545 ± 0.031

5.906 ± 0.039

2.651 ± 0.026

9.49 ± 0.04

2.257 ± 0.023

2.67 ± 0.036

1.654 ± 0.031

10.667 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.721 ± 0.016

1.495 ± 0.022

5.711 ± 0.028

2.756 ± 0.021

8.119 ± 0.045

5.098 ± 0.029

6.043 ± 0.038

10.123 ± 0.039

1.509 ± 0.019

1.812 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski