Cyphomyrmex costatus
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15716 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A151IMZ8|A0A151IMZ8_9HYME Uncharacterized protein OS=Cyphomyrmex costatus OX=456900 GN=ALC62_02640 PE=4 SV=1
MM1 pKa = 7.49 NIAPTSDD8 pKa = 3.2 EE9 pKa = 4.63 EE10 pKa = 4.57 NPFDD14 pKa = 5.7 AFLTLHH20 pKa = 6.79 NDD22 pKa = 3.32 NGHH25 pKa = 7.21 DD26 pKa = 4.17 DD27 pKa = 4.46 DD28 pKa = 6.03 LFAIINPPIIDD39 pKa = 5.14 GIQIINHH46 pKa = 5.2 VLIVNYY52 pKa = 8.07 TT53 pKa = 3.23
Molecular weight: 5.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.795
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 1.901
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A151IJL4|A0A151IJL4_9HYME Uncharacterized protein OS=Cyphomyrmex costatus OX=456900 GN=ALC62_06183 PE=4 SV=1
MM1 pKa = 7.45 ATTTTATTTHH11 pKa = 6.72 CFLQTSKK18 pKa = 11.08 HH19 pKa = 4.97 PFPRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 TRR27 pKa = 11.84 RR28 pKa = 11.84 HH29 pKa = 5.05 LLPRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 TPRR38 pKa = 11.84 HH39 pKa = 4.71 LFRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 TPRR48 pKa = 11.84 HH49 pKa = 5.27 PYY51 pKa = 8.45 PRR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 TPRR58 pKa = 11.84 HH59 pKa = 4.68 SLPRR63 pKa = 11.84 RR64 pKa = 11.84 RR65 pKa = 11.84 TPRR68 pKa = 11.84 HH69 pKa = 4.68 SLPRR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 TRR77 pKa = 11.84 RR78 pKa = 11.84 HH79 pKa = 5.81 PIPRR83 pKa = 11.84 RR84 pKa = 11.84 RR85 pKa = 11.84 TPRR88 pKa = 11.84 HH89 pKa = 4.47 LVPRR93 pKa = 11.84 RR94 pKa = 11.84 RR95 pKa = 11.84 TPRR98 pKa = 11.84 HH99 pKa = 5.43 SVPQRR104 pKa = 11.84 RR105 pKa = 11.84 TPRR108 pKa = 11.84 HH109 pKa = 4.76 SLPRR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 TPRR118 pKa = 11.84 HH119 pKa = 4.89 LVPRR123 pKa = 11.84 MRR125 pKa = 11.84 TPRR128 pKa = 11.84 HH129 pKa = 5.27 LFPRR133 pKa = 11.84 QRR135 pKa = 11.84 TPRR138 pKa = 11.84 HH139 pKa = 5.79 SLPQNRR145 pKa = 11.84 TRR147 pKa = 11.84 RR148 pKa = 11.84 HH149 pKa = 4.95 LLPRR153 pKa = 11.84 RR154 pKa = 11.84 RR155 pKa = 11.84 TYY157 pKa = 10.37 II158 pKa = 3.42
Molecular weight: 19.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.462
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.289
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15716
0
15716
7198715
18
14081
458.1
51.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.92 ± 0.019
2.084 ± 0.025
5.359 ± 0.018
6.572 ± 0.027
3.707 ± 0.018
5.168 ± 0.024
2.563 ± 0.01
6.249 ± 0.025
6.492 ± 0.028
9.038 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.268 ± 0.01
5.295 ± 0.02
4.78 ± 0.027
4.092 ± 0.024
5.907 ± 0.023
8.083 ± 0.026
6.027 ± 0.017
5.925 ± 0.014
1.08 ± 0.007
3.28 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here