WU polyomavirus (WUPyV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Betapolyomavirus; Human polyomavirus 4

Average proteome isoelectric point is 7.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A5HBE1-2|VP2-2_POVWU Isoform of A5HBE1 Isoform VP3 of Minor capsid protein VP2 OS=WU polyomavirus OX=440266 PE=3 SV=1
MM1 pKa = 8.15DD2 pKa = 3.67KK3 pKa = 10.13TLSRR7 pKa = 11.84NEE9 pKa = 4.16AKK11 pKa = 10.6EE12 pKa = 3.79LMQLLGLDD20 pKa = 3.57MTCWGNLPLMRR31 pKa = 11.84TKK33 pKa = 10.78YY34 pKa = 10.37LSKK37 pKa = 10.64CKK39 pKa = 9.91EE40 pKa = 3.7FHH42 pKa = 6.72PDD44 pKa = 2.82KK45 pKa = 11.16GGNEE49 pKa = 3.91EE50 pKa = 4.92KK51 pKa = 10.12MKK53 pKa = 10.72KK54 pKa = 10.31LNSLYY59 pKa = 10.78LKK61 pKa = 10.45LQEE64 pKa = 4.67CVSTVHH70 pKa = 6.14QLNEE74 pKa = 4.13EE75 pKa = 3.99EE76 pKa = 4.62DD77 pKa = 4.45EE78 pKa = 4.47VWSSSQIPTYY88 pKa = 8.7GTPDD92 pKa = 3.01WDD94 pKa = 4.04YY95 pKa = 10.23WWSQFNSYY103 pKa = 8.75WEE105 pKa = 4.03EE106 pKa = 3.58EE107 pKa = 4.2LRR109 pKa = 11.84CNEE112 pKa = 4.56EE113 pKa = 4.12MPKK116 pKa = 10.4SPGEE120 pKa = 4.27TPTKK124 pKa = 9.6RR125 pKa = 11.84TRR127 pKa = 11.84EE128 pKa = 3.95DD129 pKa = 3.69DD130 pKa = 4.17EE131 pKa = 5.35EE132 pKa = 4.44PQCSQATPPKK142 pKa = 9.75KK143 pKa = 10.5KK144 pKa = 9.99KK145 pKa = 10.64DD146 pKa = 3.66NATDD150 pKa = 3.52ASLSFPKK157 pKa = 10.28EE158 pKa = 3.61LEE160 pKa = 4.15EE161 pKa = 4.38FVSQAVFSNRR171 pKa = 11.84TLTAFVIHH179 pKa = 5.2TTKK182 pKa = 10.73EE183 pKa = 4.0KK184 pKa = 11.34AEE186 pKa = 4.03TLYY189 pKa = 11.18KK190 pKa = 10.61KK191 pKa = 10.53LLSKK195 pKa = 8.86FKK197 pKa = 11.03CNFASRR203 pKa = 11.84HH204 pKa = 4.86SYY206 pKa = 11.01YY207 pKa = 9.85NTALVFILTPFRR219 pKa = 11.84HH220 pKa = 5.52RR221 pKa = 11.84VSAVNNFCKK230 pKa = 10.39GYY232 pKa = 8.56CTISFLFCKK241 pKa = 10.24GVNNAYY247 pKa = 10.36GLYY250 pKa = 10.57SRR252 pKa = 11.84MTRR255 pKa = 11.84DD256 pKa = 3.26PFTLCEE262 pKa = 3.9EE263 pKa = 4.69NIPGGLKK270 pKa = 10.74EE271 pKa = 4.42NDD273 pKa = 3.48FKK275 pKa = 11.76AEE277 pKa = 3.95DD278 pKa = 3.92LYY280 pKa = 11.77GEE282 pKa = 5.23FKK284 pKa = 11.04DD285 pKa = 3.73QLNWKK290 pKa = 9.1ALSEE294 pKa = 4.19FALEE298 pKa = 4.4LGIDD302 pKa = 3.86DD303 pKa = 4.83VYY305 pKa = 11.82LLLGLYY311 pKa = 9.53LQLSIKK317 pKa = 10.48VEE319 pKa = 4.04EE320 pKa = 4.47CEE322 pKa = 4.1KK323 pKa = 11.13CNSNEE328 pKa = 4.03DD329 pKa = 3.37ATHH332 pKa = 6.02NRR334 pKa = 11.84LHH336 pKa = 6.52MEE338 pKa = 3.95HH339 pKa = 6.28QKK341 pKa = 10.75NALLFSDD348 pKa = 4.63SKK350 pKa = 10.58SQKK353 pKa = 9.84NVCQQAIDD361 pKa = 3.6VVIAKK366 pKa = 10.07RR367 pKa = 11.84RR368 pKa = 11.84VDD370 pKa = 3.72SLNMSRR376 pKa = 11.84EE377 pKa = 4.02DD378 pKa = 3.25LLARR382 pKa = 11.84RR383 pKa = 11.84FEE385 pKa = 4.73KK386 pKa = 10.62ILDD389 pKa = 3.97KK390 pKa = 10.18MDD392 pKa = 3.31KK393 pKa = 9.68TIKK396 pKa = 10.72GEE398 pKa = 4.05QDD400 pKa = 3.12VLLYY404 pKa = 9.12MAGVAWYY411 pKa = 9.99LGLNGKK417 pKa = 9.09IDD419 pKa = 3.84EE420 pKa = 4.33LVYY423 pKa = 10.41RR424 pKa = 11.84YY425 pKa = 10.56LKK427 pKa = 10.76VIVEE431 pKa = 4.24NVPKK435 pKa = 10.22KK436 pKa = 10.46RR437 pKa = 11.84YY438 pKa = 8.27WVFKK442 pKa = 10.85GPINSGKK449 pKa = 6.84TTVAAALLDD458 pKa = 3.9LCGGKK463 pKa = 9.81ALNINIPADD472 pKa = 3.71RR473 pKa = 11.84LNFEE477 pKa = 5.01LGVAIDD483 pKa = 3.39QFTVVFEE490 pKa = 4.28DD491 pKa = 3.34VKK493 pKa = 11.44GQVGDD498 pKa = 3.88NKK500 pKa = 10.6LLPSGNGMSNLDD512 pKa = 3.72NLRR515 pKa = 11.84DD516 pKa = 3.83YY517 pKa = 11.6LDD519 pKa = 4.13GSVKK523 pKa = 10.37VNLEE527 pKa = 3.88KK528 pKa = 10.8KK529 pKa = 9.64HH530 pKa = 5.73LNKK533 pKa = 10.08RR534 pKa = 11.84SQIFPPGIVTMNEE547 pKa = 3.84YY548 pKa = 10.54LVPATLAPRR557 pKa = 11.84FHH559 pKa = 6.4KK560 pKa = 9.6TVLFTPKK567 pKa = 9.88RR568 pKa = 11.84HH569 pKa = 6.07LKK571 pKa = 10.32EE572 pKa = 4.64SLDD575 pKa = 3.63KK576 pKa = 11.12TPEE579 pKa = 3.96LMVKK583 pKa = 10.31RR584 pKa = 11.84VLQSGMCILLMLIWCRR600 pKa = 11.84PVSDD604 pKa = 4.39FHH606 pKa = 7.77PCIQAKK612 pKa = 8.84VVYY615 pKa = 9.2WKK617 pKa = 10.62EE618 pKa = 3.94LLDD621 pKa = 3.9KK622 pKa = 11.22YY623 pKa = 10.92IGLTEE628 pKa = 4.07FADD631 pKa = 3.43MQMNVTNGCNILEE644 pKa = 4.21KK645 pKa = 10.92HH646 pKa = 5.58NAA648 pKa = 3.31

Molecular weight: 74.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A5HBE1-2|VP2-2_POVWU Isoform of A5HBE1 Isoform VP3 of Minor capsid protein VP2 OS=WU polyomavirus OX=440266 PE=3 SV=1
MM1 pKa = 7.61ALAPIPEE8 pKa = 4.39YY9 pKa = 10.8NLEE12 pKa = 4.07TGIPGVPDD20 pKa = 3.05WVFNFIASHH29 pKa = 5.87LPEE32 pKa = 5.33LPSLQDD38 pKa = 3.05VFNRR42 pKa = 11.84IAYY45 pKa = 7.47GIWTSYY51 pKa = 10.35YY52 pKa = 7.9NTGRR56 pKa = 11.84TVVNRR61 pKa = 11.84AVSEE65 pKa = 3.97EE66 pKa = 3.96LQRR69 pKa = 11.84LLGDD73 pKa = 3.59LEE75 pKa = 4.56YY76 pKa = 11.22GFRR79 pKa = 11.84TALATIGEE87 pKa = 4.54SDD89 pKa = 3.83PVNAIVEE96 pKa = 4.38QVRR99 pKa = 11.84SFVSGGRR106 pKa = 11.84QRR108 pKa = 11.84EE109 pKa = 4.13LLQIAAGQPVDD120 pKa = 3.09ISEE123 pKa = 4.26GVSRR127 pKa = 11.84GTATISNAVEE137 pKa = 3.96AVRR140 pKa = 11.84DD141 pKa = 3.67ATQRR145 pKa = 11.84LSQATYY151 pKa = 10.27NFVYY155 pKa = 10.39DD156 pKa = 4.26ASTLPRR162 pKa = 11.84DD163 pKa = 3.84GFNALSDD170 pKa = 3.79GVHH173 pKa = 7.37RR174 pKa = 11.84LGQWISMPGATGGTPHH190 pKa = 6.65YY191 pKa = 9.8AAPDD195 pKa = 3.69WILYY199 pKa = 9.04VLEE202 pKa = 4.79EE203 pKa = 4.48LNSDD207 pKa = 2.93ISKK210 pKa = 10.48IPTQGIKK217 pKa = 10.29RR218 pKa = 11.84KK219 pKa = 8.7LQQNGLHH226 pKa = 6.28SKK228 pKa = 10.93ASLHH232 pKa = 5.72SKK234 pKa = 6.67TRR236 pKa = 11.84KK237 pKa = 6.24VTKK240 pKa = 10.4KK241 pKa = 8.99STHH244 pKa = 6.19KK245 pKa = 10.24SAKK248 pKa = 9.15PSKK251 pKa = 8.8TSQKK255 pKa = 9.88RR256 pKa = 11.84RR257 pKa = 11.84GRR259 pKa = 11.84RR260 pKa = 11.84AGRR263 pKa = 11.84RR264 pKa = 11.84TTVRR268 pKa = 11.84RR269 pKa = 11.84NRR271 pKa = 11.84VV272 pKa = 2.93

Molecular weight: 30.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1898

194

648

379.6

42.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.902 ± 1.544

2.002 ± 0.911

4.268 ± 0.572

6.428 ± 0.775

3.319 ± 0.651

7.376 ± 1.193

1.897 ± 0.16

4.426 ± 0.484

6.375 ± 1.414

10.063 ± 0.751

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.844 ± 0.506

4.953 ± 0.643

5.532 ± 0.899

3.793 ± 0.276

5.374 ± 0.779

6.481 ± 0.617

6.744 ± 0.819

7.06 ± 0.719

1.528 ± 0.266

3.635 ± 0.475
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski