Cronartium ribicola mitovirus 4
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191KCP1|A0A191KCP1_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 4 OX=1816487 GN=RdRp PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.63 LIINIMKK9 pKa = 9.69 IWAQSKK15 pKa = 10.17 RR16 pKa = 11.84 FLQIAKK22 pKa = 8.67 TFYY25 pKa = 8.4 TWYY28 pKa = 9.68 SVYY31 pKa = 10.77 YY32 pKa = 6.13 PTCSPDD38 pKa = 4.25 DD39 pKa = 3.21 IAPFLEE45 pKa = 4.5 RR46 pKa = 11.84 VHH48 pKa = 6.88 HH49 pKa = 6.03 SLANRR54 pKa = 11.84 GALATLEE61 pKa = 4.13 WLKK64 pKa = 9.19 EE65 pKa = 3.82 TRR67 pKa = 11.84 LAFTRR72 pKa = 11.84 WICGDD77 pKa = 3.67 SRR79 pKa = 11.84 PDD81 pKa = 3.17 VCVKK85 pKa = 10.42 LDD87 pKa = 3.68 SSGLPVMVKK96 pKa = 8.84 STLGKK101 pKa = 10.5 FIDD104 pKa = 3.93 TARR107 pKa = 11.84 GRR109 pKa = 11.84 ALILTLLNCIRR120 pKa = 11.84 SYY122 pKa = 11.33 EE123 pKa = 4.09 GLKK126 pKa = 10.37 EE127 pKa = 3.93 PSISDD132 pKa = 2.85 ITSPYY137 pKa = 10.03 KK138 pKa = 9.87 GEE140 pKa = 3.84 KK141 pKa = 9.38 WIDD144 pKa = 3.52 SYY146 pKa = 11.9 LGLYY150 pKa = 8.75 TKK152 pKa = 10.81 DD153 pKa = 3.64 FLRR156 pKa = 11.84 KK157 pKa = 9.59 YY158 pKa = 10.92 GDD160 pKa = 2.92 IHH162 pKa = 7.43 RR163 pKa = 11.84 PRR165 pKa = 11.84 PQFKK169 pKa = 9.21 QWLYY173 pKa = 4.77 TTKK176 pKa = 10.37 RR177 pKa = 11.84 GPNGPSIATAYY188 pKa = 10.65 HH189 pKa = 7.57 DD190 pKa = 3.29 ITLLTEE196 pKa = 3.45 EE197 pKa = 5.03 HH198 pKa = 5.69 YY199 pKa = 10.7 RR200 pKa = 11.84 IYY202 pKa = 10.83 EE203 pKa = 3.98 VLAGKK208 pKa = 9.8 DD209 pKa = 3.72 YY210 pKa = 11.16 KK211 pKa = 11.18 DD212 pKa = 3.42 SLLKK216 pKa = 10.4 IKK218 pKa = 10.26 EE219 pKa = 4.18 IASDD223 pKa = 3.5 PQEE226 pKa = 3.95 FEE228 pKa = 4.9 RR229 pKa = 11.84 IRR231 pKa = 11.84 KK232 pKa = 9.23 DD233 pKa = 3.08 FSLIVDD239 pKa = 3.8 IKK241 pKa = 10.57 SRR243 pKa = 11.84 YY244 pKa = 6.84 VEE246 pKa = 4.58 KK247 pKa = 10.63 ISVIPSPEE255 pKa = 3.51 GKK257 pKa = 8.79 MRR259 pKa = 11.84 TISIASYY266 pKa = 8.65 WAQNALDD273 pKa = 4.33 PLHH276 pKa = 6.91 RR277 pKa = 11.84 CIFKK281 pKa = 10.37 YY282 pKa = 10.27 LRR284 pKa = 11.84 KK285 pKa = 9.54 IPNDD289 pKa = 2.89 ITFDD293 pKa = 3.52 QSKK296 pKa = 10.32 GLSILNKK303 pKa = 9.66 GQNDD307 pKa = 4.53 SYY309 pKa = 11.11 WSYY312 pKa = 11.6 DD313 pKa = 3.23 LKK315 pKa = 11.16 AATDD319 pKa = 3.88 RR320 pKa = 11.84 FPRR323 pKa = 11.84 STQTLLLSHH332 pKa = 7.51 LIGQEE337 pKa = 4.35 FANTWSLLISAEE349 pKa = 3.84 HH350 pKa = 7.51 DD351 pKa = 3.11 IPAIGIRR358 pKa = 11.84 AAWAVGQPIGKK369 pKa = 9.2 LSSWAVFAYY378 pKa = 7.38 THH380 pKa = 6.33 HH381 pKa = 6.61 FIIYY385 pKa = 7.55 VAHH388 pKa = 6.99 RR389 pKa = 11.84 RR390 pKa = 11.84 ACVPFAPGTYY400 pKa = 10.06 VILGDD405 pKa = 4.33 DD406 pKa = 3.66 VVIANDD412 pKa = 3.75 KK413 pKa = 10.43 VAEE416 pKa = 4.16 QYY418 pKa = 8.64 STLIRR423 pKa = 11.84 WLGVEE428 pKa = 3.41 ISMNKK433 pKa = 6.13 THH435 pKa = 7.2 RR436 pKa = 11.84 SSHH439 pKa = 5.05 SFEE442 pKa = 3.73 IGKK445 pKa = 9.53 RR446 pKa = 11.84 WIHH449 pKa = 6.41 RR450 pKa = 11.84 GQEE453 pKa = 3.6 ISPFSIVPWMQAKK466 pKa = 9.37 NWAPAITQCIYY477 pKa = 10.66 EE478 pKa = 4.19 EE479 pKa = 4.63 KK480 pKa = 10.04 KK481 pKa = 10.81 RR482 pKa = 11.84 SWSLPFHH489 pKa = 6.12 PVEE492 pKa = 4.38 GVARR496 pKa = 11.84 CILLQYY502 pKa = 9.86 HH503 pKa = 6.86 GKK505 pKa = 10.31 LSSRR509 pKa = 11.84 YY510 pKa = 8.54 IDD512 pKa = 3.6 HH513 pKa = 7.28 LFDD516 pKa = 5.22 LSQLNVAVYY525 pKa = 9.84 HH526 pKa = 6.35 SIKK529 pKa = 10.83 EE530 pKa = 4.03 MITWSDD536 pKa = 4.16 CINIIAEE543 pKa = 4.24 VFYY546 pKa = 9.4 KK547 pKa = 9.79 TKK549 pKa = 10.0 WEE551 pKa = 4.12 HH552 pKa = 6.75 LDD554 pKa = 3.49 RR555 pKa = 11.84 LHH557 pKa = 6.92 GRR559 pKa = 11.84 LVQTYY564 pKa = 9.57 VYY566 pKa = 10.86 LRR568 pKa = 11.84 LFNKK572 pKa = 9.88 NAIPHH577 pKa = 5.98 WSAIKK582 pKa = 10.36 KK583 pKa = 10.14 VILVEE588 pKa = 4.34 NGFSMASIIQIIMGGRR604 pKa = 11.84 SLSGASFSYY613 pKa = 11.39 ADD615 pKa = 4.58 LPHH618 pKa = 6.57 KK619 pKa = 10.54 KK620 pKa = 9.7 IIRR623 pKa = 11.84 RR624 pKa = 11.84 YY625 pKa = 8.3 AQEE628 pKa = 4.55 FFDD631 pKa = 4.31 KK632 pKa = 11.12 NADD635 pKa = 3.61 VVQGHH640 pKa = 5.4 YY641 pKa = 10.68 FEE643 pKa = 7.28 DD644 pKa = 3.84 GMLQRR649 pKa = 11.84 EE650 pKa = 4.6 AVRR653 pKa = 11.84 SLGIPNVEE661 pKa = 4.02 HH662 pKa = 7.27 AFTKK666 pKa = 10.17 SKK668 pKa = 10.22 VRR670 pKa = 11.84 RR671 pKa = 11.84 EE672 pKa = 4.21 VILSNNILKK681 pKa = 9.37 EE682 pKa = 3.7 LCSFVKK688 pKa = 10.36 SVPSSGFTHH697 pKa = 6.99 TSGSPVPTEE706 pKa = 3.26 INEE709 pKa = 4.34 YY710 pKa = 8.9 IRR712 pKa = 11.84 KK713 pKa = 8.31 VNYY716 pKa = 9.8 YY717 pKa = 10.05 EE718 pKa = 4.01 WDD720 pKa = 3.3 TT721 pKa = 3.76
Molecular weight: 83.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.714
IPC2_protein 8.697
IPC_protein 8.58
Toseland 9.136
ProMoST 9.048
Dawson 9.487
Bjellqvist 9.297
Wikipedia 9.677
Rodwell 9.663
Grimsley 9.575
Solomon 9.516
Lehninger 9.458
Nozaki 9.341
DTASelect 9.238
Thurlkill 9.326
EMBOSS 9.604
Sillero 9.472
Patrickios 4.507
IPC_peptide 9.502
IPC2_peptide 7.98
IPC2.peptide.svr19 7.742
Protein with the highest isoelectric point:
>tr|A0A191KCP1|A0A191KCP1_9VIRU RNA-dependent RNA polymerase OS=Cronartium ribicola mitovirus 4 OX=1816487 GN=RdRp PE=4 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.63 LIINIMKK9 pKa = 9.69 IWAQSKK15 pKa = 10.17 RR16 pKa = 11.84 FLQIAKK22 pKa = 8.67 TFYY25 pKa = 8.4 TWYY28 pKa = 9.68 SVYY31 pKa = 10.77 YY32 pKa = 6.13 PTCSPDD38 pKa = 4.25 DD39 pKa = 3.21 IAPFLEE45 pKa = 4.5 RR46 pKa = 11.84 VHH48 pKa = 6.88 HH49 pKa = 6.03 SLANRR54 pKa = 11.84 GALATLEE61 pKa = 4.13 WLKK64 pKa = 9.19 EE65 pKa = 3.82 TRR67 pKa = 11.84 LAFTRR72 pKa = 11.84 WICGDD77 pKa = 3.67 SRR79 pKa = 11.84 PDD81 pKa = 3.17 VCVKK85 pKa = 10.42 LDD87 pKa = 3.68 SSGLPVMVKK96 pKa = 8.84 STLGKK101 pKa = 10.5 FIDD104 pKa = 3.93 TARR107 pKa = 11.84 GRR109 pKa = 11.84 ALILTLLNCIRR120 pKa = 11.84 SYY122 pKa = 11.33 EE123 pKa = 4.09 GLKK126 pKa = 10.37 EE127 pKa = 3.93 PSISDD132 pKa = 2.85 ITSPYY137 pKa = 10.03 KK138 pKa = 9.87 GEE140 pKa = 3.84 KK141 pKa = 9.38 WIDD144 pKa = 3.52 SYY146 pKa = 11.9 LGLYY150 pKa = 8.75 TKK152 pKa = 10.81 DD153 pKa = 3.64 FLRR156 pKa = 11.84 KK157 pKa = 9.59 YY158 pKa = 10.92 GDD160 pKa = 2.92 IHH162 pKa = 7.43 RR163 pKa = 11.84 PRR165 pKa = 11.84 PQFKK169 pKa = 9.21 QWLYY173 pKa = 4.77 TTKK176 pKa = 10.37 RR177 pKa = 11.84 GPNGPSIATAYY188 pKa = 10.65 HH189 pKa = 7.57 DD190 pKa = 3.29 ITLLTEE196 pKa = 3.45 EE197 pKa = 5.03 HH198 pKa = 5.69 YY199 pKa = 10.7 RR200 pKa = 11.84 IYY202 pKa = 10.83 EE203 pKa = 3.98 VLAGKK208 pKa = 9.8 DD209 pKa = 3.72 YY210 pKa = 11.16 KK211 pKa = 11.18 DD212 pKa = 3.42 SLLKK216 pKa = 10.4 IKK218 pKa = 10.26 EE219 pKa = 4.18 IASDD223 pKa = 3.5 PQEE226 pKa = 3.95 FEE228 pKa = 4.9 RR229 pKa = 11.84 IRR231 pKa = 11.84 KK232 pKa = 9.23 DD233 pKa = 3.08 FSLIVDD239 pKa = 3.8 IKK241 pKa = 10.57 SRR243 pKa = 11.84 YY244 pKa = 6.84 VEE246 pKa = 4.58 KK247 pKa = 10.63 ISVIPSPEE255 pKa = 3.51 GKK257 pKa = 8.79 MRR259 pKa = 11.84 TISIASYY266 pKa = 8.65 WAQNALDD273 pKa = 4.33 PLHH276 pKa = 6.91 RR277 pKa = 11.84 CIFKK281 pKa = 10.37 YY282 pKa = 10.27 LRR284 pKa = 11.84 KK285 pKa = 9.54 IPNDD289 pKa = 2.89 ITFDD293 pKa = 3.52 QSKK296 pKa = 10.32 GLSILNKK303 pKa = 9.66 GQNDD307 pKa = 4.53 SYY309 pKa = 11.11 WSYY312 pKa = 11.6 DD313 pKa = 3.23 LKK315 pKa = 11.16 AATDD319 pKa = 3.88 RR320 pKa = 11.84 FPRR323 pKa = 11.84 STQTLLLSHH332 pKa = 7.51 LIGQEE337 pKa = 4.35 FANTWSLLISAEE349 pKa = 3.84 HH350 pKa = 7.51 DD351 pKa = 3.11 IPAIGIRR358 pKa = 11.84 AAWAVGQPIGKK369 pKa = 9.2 LSSWAVFAYY378 pKa = 7.38 THH380 pKa = 6.33 HH381 pKa = 6.61 FIIYY385 pKa = 7.55 VAHH388 pKa = 6.99 RR389 pKa = 11.84 RR390 pKa = 11.84 ACVPFAPGTYY400 pKa = 10.06 VILGDD405 pKa = 4.33 DD406 pKa = 3.66 VVIANDD412 pKa = 3.75 KK413 pKa = 10.43 VAEE416 pKa = 4.16 QYY418 pKa = 8.64 STLIRR423 pKa = 11.84 WLGVEE428 pKa = 3.41 ISMNKK433 pKa = 6.13 THH435 pKa = 7.2 RR436 pKa = 11.84 SSHH439 pKa = 5.05 SFEE442 pKa = 3.73 IGKK445 pKa = 9.53 RR446 pKa = 11.84 WIHH449 pKa = 6.41 RR450 pKa = 11.84 GQEE453 pKa = 3.6 ISPFSIVPWMQAKK466 pKa = 9.37 NWAPAITQCIYY477 pKa = 10.66 EE478 pKa = 4.19 EE479 pKa = 4.63 KK480 pKa = 10.04 KK481 pKa = 10.81 RR482 pKa = 11.84 SWSLPFHH489 pKa = 6.12 PVEE492 pKa = 4.38 GVARR496 pKa = 11.84 CILLQYY502 pKa = 9.86 HH503 pKa = 6.86 GKK505 pKa = 10.31 LSSRR509 pKa = 11.84 YY510 pKa = 8.54 IDD512 pKa = 3.6 HH513 pKa = 7.28 LFDD516 pKa = 5.22 LSQLNVAVYY525 pKa = 9.84 HH526 pKa = 6.35 SIKK529 pKa = 10.83 EE530 pKa = 4.03 MITWSDD536 pKa = 4.16 CINIIAEE543 pKa = 4.24 VFYY546 pKa = 9.4 KK547 pKa = 9.79 TKK549 pKa = 10.0 WEE551 pKa = 4.12 HH552 pKa = 6.75 LDD554 pKa = 3.49 RR555 pKa = 11.84 LHH557 pKa = 6.92 GRR559 pKa = 11.84 LVQTYY564 pKa = 9.57 VYY566 pKa = 10.86 LRR568 pKa = 11.84 LFNKK572 pKa = 9.88 NAIPHH577 pKa = 5.98 WSAIKK582 pKa = 10.36 KK583 pKa = 10.14 VILVEE588 pKa = 4.34 NGFSMASIIQIIMGGRR604 pKa = 11.84 SLSGASFSYY613 pKa = 11.39 ADD615 pKa = 4.58 LPHH618 pKa = 6.57 KK619 pKa = 10.54 KK620 pKa = 9.7 IIRR623 pKa = 11.84 RR624 pKa = 11.84 YY625 pKa = 8.3 AQEE628 pKa = 4.55 FFDD631 pKa = 4.31 KK632 pKa = 11.12 NADD635 pKa = 3.61 VVQGHH640 pKa = 5.4 YY641 pKa = 10.68 FEE643 pKa = 7.28 DD644 pKa = 3.84 GMLQRR649 pKa = 11.84 EE650 pKa = 4.6 AVRR653 pKa = 11.84 SLGIPNVEE661 pKa = 4.02 HH662 pKa = 7.27 AFTKK666 pKa = 10.17 SKK668 pKa = 10.22 VRR670 pKa = 11.84 RR671 pKa = 11.84 EE672 pKa = 4.21 VILSNNILKK681 pKa = 9.37 EE682 pKa = 3.7 LCSFVKK688 pKa = 10.36 SVPSSGFTHH697 pKa = 6.99 TSGSPVPTEE706 pKa = 3.26 INEE709 pKa = 4.34 YY710 pKa = 8.9 IRR712 pKa = 11.84 KK713 pKa = 8.31 VNYY716 pKa = 9.8 YY717 pKa = 10.05 EE718 pKa = 4.01 WDD720 pKa = 3.3 TT721 pKa = 3.76
Molecular weight: 83.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.714
IPC2_protein 8.697
IPC_protein 8.58
Toseland 9.136
ProMoST 9.048
Dawson 9.487
Bjellqvist 9.297
Wikipedia 9.677
Rodwell 9.663
Grimsley 9.575
Solomon 9.516
Lehninger 9.458
Nozaki 9.341
DTASelect 9.238
Thurlkill 9.326
EMBOSS 9.604
Sillero 9.472
Patrickios 4.507
IPC_peptide 9.502
IPC2_peptide 7.98
IPC2.peptide.svr19 7.742
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
721
721
721
721.0
83.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.38 ± 0.0
1.387 ± 0.0
4.854 ± 0.0
4.993 ± 0.0
4.161 ± 0.0
4.993 ± 0.0
3.467 ± 0.0
9.57 ± 0.0
6.935 ± 0.0
8.599 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.387 ± 0.0
3.19 ± 0.0
4.438 ± 0.0
3.051 ± 0.0
5.964 ± 0.0
8.599 ± 0.0
4.854 ± 0.0
5.409 ± 0.0
2.774 ± 0.0
4.993 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here